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The map label for this gene is 75674329

Identifier: 75674329

GI number: 75674329

Start: 150408

End: 151742

Strand: Direct

Name: 75674329

Synonym: Nwi_0130

Alternate gene names: NA

Gene position: 150408-151742 (Clockwise)

Preceding gene: 75674328

Following gene: 75674330

Centisome position: 4.42

GC content: 61.12

Gene sequence:

>1335_bases
ATGCCGATCGGTGAATTTGGACGGCCTCCGGAGTTGCCGGCCGAGGTCAGCCCGACGATGACGGCGCCGATGTACTGGAT
GTACGAAATGGCCCAGGCCTCGCTCAATCCGGCCCGCGCCATCACTGACGCGACCCGGATGCTGTTCCAAAACCCGCTCA
ATCCGTGGGCGCATACCCAGCTCGGCAAATCTGTCGCCGCGGGTTGCGAACTGTTCGAGCGCACCACCCGCCGTTACGGA
AAACCGGAATGGGGTCTCCACGAGACCAAAGTCAACGGCGTGCGTACGCCGATCGAGATCCGCTCGGTTTGGGAGAAGCC
CTTCTGTCGTCTGCTCTACTTCGATCGGATGCACCCGCGCCCGCTGCGGACGCCGCAGCCGCGCCTGTTGCTGGTGGCGC
CGATGTCGGGTCACTACGCGACCTTGCTGCGCGGAACCGTCGAAGCGTTCATGCCGACGCACGACGTCTATATCACCGAC
TGGTCCGACGCCCGCATGGTTCCGGTGGCGGAAGGTCGTTTCGATCTGGATGATTTCGTCGACTATCTGATCGAGATCCT
CCACATGCTCGGTGGAAACGTTCATGTCATCGCCGTCTGCCAGCCGTCGGTCCCGGTGCTGGCCGCGGTGTCGGCGATGG
AGGCCGCGCGCGATCCCTATGTTCCGCTGTCGATGACGTTGATGGGAGGGCCGATCGATACTCGCAAGAATCCGACCGCC
GTCAACAATCTCGCCGCCGAGAGAGGCACCGATTGGTTCCGCAATAACGTCATCACCAAGGTGCCGTTTCCGCATCCCGG
CGTAATGCGCGACGTCTATCCCGGCTTCTTGCAGCTCAACGGCTTCATCAGCATGAATTTCGATCGCCACATGAATGCGC
ACAAGAGCCTGTTCGAGCATCTCGTCAAAGGCGACGGCGACCTGGCCGACAAGCACCGCGATTTCTATGACGAGTATCTC
GCTGTCATGGACCTGACCGCGGAGTATTATCTGCAAACGGTCGATCAGGTGTTCGTCAAACACGCTTTGCCGAAGGGCGA
GATGACTCACCGCGGCCGGCTGGTCGATCCTTCGAAGATCAAGCGCGTCGCGCTGATGACGGTGGAGGGCGAGAAGGACG
ACATTTCCGGCCTCGGCCAGACCGAAGCTGCGCATGCGTTATGCACATCGATCCCGGACAACCGGCGAACCCATTACGTG
CAGAGCGGCGTCGGTCACTATGGCGTGTTTAACGGTTCGAGATTCAGGTCGGAGATCGTCCCGCGCATGAATGACTTCAT
GCAGTCGAACGCCGGGCCTCCGGAGAAAGCATCGCGTGCTTCGGCCGCGGAATGA

Upstream 100 bases:

>100_bases
CTCTAAAGAGATCAACCCACTATATGTTGGATAGCGGTAGATTGACGTCATGGACCGTGGCGGTCCATCATGCTGCGGTG
CAAAAAAGAGGAACAGGCCA

Downstream 100 bases:

>100_bases
ATCGCGCAATCCAAGCGGAAATCGATCGGGCCGCCTGATCCAGAGCGCGGCCATCGTGCTGGATAACCGGCCTGTTTCGA
CCTTTCTCAAAGAATGAACC

Product: polyhydroxyalkanoate depolymerase

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly(3-Hydroxyalkanoate)Synthetase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Polyhydroxybutanoate Depolymerase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPIGEFGRPPELPAEVSPTMTAPMYWMYEMAQASLNPARAITDATRMLFQNPLNPWAHTQLGKSVAAGCELFERTTRRYG
KPEWGLHETKVNGVRTPIEIRSVWEKPFCRLLYFDRMHPRPLRTPQPRLLLVAPMSGHYATLLRGTVEAFMPTHDVYITD
WSDARMVPVAEGRFDLDDFVDYLIEILHMLGGNVHVIAVCQPSVPVLAAVSAMEAARDPYVPLSMTLMGGPIDTRKNPTA
VNNLAAERGTDWFRNNVITKVPFPHPGVMRDVYPGFLQLNGFISMNFDRHMNAHKSLFEHLVKGDGDLADKHRDFYDEYL
AVMDLTAEYYLQTVDQVFVKHALPKGEMTHRGRLVDPSKIKRVALMTVEGEKDDISGLGQTEAAHALCTSIPDNRRTHYV
QSGVGHYGVFNGSRFRSEIVPRMNDFMQSNAGPPEKASRASAAE

Sequences:

>Translated_444_residues
MPIGEFGRPPELPAEVSPTMTAPMYWMYEMAQASLNPARAITDATRMLFQNPLNPWAHTQLGKSVAAGCELFERTTRRYG
KPEWGLHETKVNGVRTPIEIRSVWEKPFCRLLYFDRMHPRPLRTPQPRLLLVAPMSGHYATLLRGTVEAFMPTHDVYITD
WSDARMVPVAEGRFDLDDFVDYLIEILHMLGGNVHVIAVCQPSVPVLAAVSAMEAARDPYVPLSMTLMGGPIDTRKNPTA
VNNLAAERGTDWFRNNVITKVPFPHPGVMRDVYPGFLQLNGFISMNFDRHMNAHKSLFEHLVKGDGDLADKHRDFYDEYL
AVMDLTAEYYLQTVDQVFVKHALPKGEMTHRGRLVDPSKIKRVALMTVEGEKDDISGLGQTEAAHALCTSIPDNRRTHYV
QSGVGHYGVFNGSRFRSEIVPRMNDFMQSNAGPPEKASRASAAE
>Mature_443_residues
PIGEFGRPPELPAEVSPTMTAPMYWMYEMAQASLNPARAITDATRMLFQNPLNPWAHTQLGKSVAAGCELFERTTRRYGK
PEWGLHETKVNGVRTPIEIRSVWEKPFCRLLYFDRMHPRPLRTPQPRLLLVAPMSGHYATLLRGTVEAFMPTHDVYITDW
SDARMVPVAEGRFDLDDFVDYLIEILHMLGGNVHVIAVCQPSVPVLAAVSAMEAARDPYVPLSMTLMGGPIDTRKNPTAV
NNLAAERGTDWFRNNVITKVPFPHPGVMRDVYPGFLQLNGFISMNFDRHMNAHKSLFEHLVKGDGDLADKHRDFYDEYLA
VMDLTAEYYLQTVDQVFVKHALPKGEMTHRGRLVDPSKIKRVALMTVEGEKDDISGLGQTEAAHALCTSIPDNRRTHYVQ
SGVGHYGVFNGSRFRSEIVPRMNDFMQSNAGPPEKASRASAAE

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 49923; Mature: 49792

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIGEFGRPPELPAEVSPTMTAPMYWMYEMAQASLNPARAITDATRMLFQNPLNPWAHTQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
LGKSVAAGCELFERTTRRYGKPEWGLHETKVNGVRTPIEIRSVWEKPFCRLLYFDRMHPR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PLRTPQPRLLLVAPMSGHYATLLRGTVEAFMPTHDVYITDWSDARMVPVAEGRFDLDDFV
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCHHHHH
DYLIEILHMLGGNVHVIAVCQPSVPVLAAVSAMEAARDPYVPLSMTLMGGPIDTRKNPTA
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
VNNLAAERGTDWFRNNVITKVPFPHPGVMRDVYPGFLQLNGFISMNFDRHMNAHKSLFEH
HHHHHHHCCCHHHHCCCEEECCCCCCCCHHHHCCCCEEECCEEEECHHHHCHHHHHHHHH
LVKGDGDLADKHRDFYDEYLAVMDLTAEYYLQTVDQVFVKHALPKGEMTHRGRLVDPSKI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
KRVALMTVEGEKDDISGLGQTEAAHALCTSIPDNRRTHYVQSGVGHYGVFNGSRFRSEIV
CEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHH
PRMNDFMQSNAGPPEKASRASAAE
HHHHHHHHCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
PIGEFGRPPELPAEVSPTMTAPMYWMYEMAQASLNPARAITDATRMLFQNPLNPWAHTQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
LGKSVAAGCELFERTTRRYGKPEWGLHETKVNGVRTPIEIRSVWEKPFCRLLYFDRMHPR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PLRTPQPRLLLVAPMSGHYATLLRGTVEAFMPTHDVYITDWSDARMVPVAEGRFDLDDFV
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCHHHHH
DYLIEILHMLGGNVHVIAVCQPSVPVLAAVSAMEAARDPYVPLSMTLMGGPIDTRKNPTA
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCH
VNNLAAERGTDWFRNNVITKVPFPHPGVMRDVYPGFLQLNGFISMNFDRHMNAHKSLFEH
HHHHHHHCCCHHHHCCCEEECCCCCCCCHHHHCCCCEEECCEEEECHHHHCHHHHHHHHH
LVKGDGDLADKHRDFYDEYLAVMDLTAEYYLQTVDQVFVKHALPKGEMTHRGRLVDPSKI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
KRVALMTVEGEKDDISGLGQTEAAHALCTSIPDNRRTHYVQSGVGHYGVFNGSRFRSEIV
CEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHH
PRMNDFMQSNAGPPEKASRASAAE
HHHHHHHHCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA