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The map label for this gene is pnp [H]

Identifier: 75674227

GI number: 75674227

Start: 29730

End: 31886

Strand: Direct

Name: pnp [H]

Synonym: Nwi_0028

Alternate gene names: 75674227

Gene position: 29730-31886 (Clockwise)

Preceding gene: 75674226

Following gene: 75674229

Centisome position: 0.87

GC content: 65.69

Gene sequence:

>2157_bases
ATGTTCAATCAACACTCCGTTGAAATCGATTGGGGCGGACGTCCCCTCAAGCTTGAAACCGGCAAGATCGCGCGCCAGGC
CGACGGCGCCGTCGTCGCCACCTACGGCGAGACCGTCGTGCTCGCCACCGTGGTCGCCGCCAAGTCGCCGCGCGAGGGCG
TCGACTTCCTGCCGCTGACCGTCGACTACCAGGAAAAGACCTATGCGGCCGGCCGCATCCCCGGCGGTTACTTCAAACGC
GAAGGCCGCCCGACCGAGAAGGAGACGCTGGTCTCCCGCCTGATCGACCGCCCGGTCCGCCCGCTGTTCGCCGATGGCTG
GCGCAACGAGACCCAGGTGATTGTGACCGTGCTGTCGCACGACATGGAGAACGACCCGGACATCCTGGCGATGGTCGCCG
CCTCGGCGGCGCTGACGCTATCCGGCGCGCCGTTCAAGGGCCCGATCGGCGCCGCCCGCGTCGGCTTCATCAATGATGAA
TACGTGCTCAACCCGACGCTCGACGAAATCGTCGACACCCAGCTCGATCTGGTCGTCGCCGGCACGGCGGACGCGGTGCT
GATGGTCGAGTCCGAGGCCAAGGAGCTTTCGGAAGAGATCATGCTCAAGGCGGTGATGTTCGGCCACCGCCACTTCCAGC
CGGTGATCGACGCGATCATCGAACTCGCCGAGAAGGCCGCCAAGGAACCGCGCGAACTCAAGGTCATCGACGATACCGAG
ATCGAGAAGGAGATGCTCGGCCTGGTCGAGCAGGATCTGCGCGCGGCCTACGCCATTCCCGTCAAGCAGGACCGCTACGC
GGCGGTCGGCAAGGTCAAGGAGAAGGCGCTTGCGCACTTCTTCCCGGAAGGCCAGGAGCCGAAATACGACAAGCTGCGCG
TCGCCGGCGTGTTCAAGGAACTCGAGGCCAAGATCGTTCGCTGGAACATCCTCGACACCGGCAAGCGCATCGACGGCCGC
GACGTCAAGACGGTGCGCAATATCGTCGCCGAGGCCGGCGTGCTGCCGCGCGCCCACGGTTCGGCGCTGTTCACCCGCGG
CGAGACCCAGGCGATGGTGGTGACCACGCTCGGCACCGGCGAGGACGAGCAGTATATCGATTCGCTGGCCGGGACGTACA
AGGAGACGTTCCTGCTGCACTACAACTTCCCGCCCTATTCGGTCGGCGAGACCGGCCGCCTCGGCGGCGCCAAGCGCCGC
GAGATCGGCCACGGCAAGCTGGCGTGGCGCGCGATCCGCCCAGTGCTGCCGCCGCATCACGAGTTTCCCTACACCATCCG
CGTCGTCTCGGAGATCACCGAGTCGAACGGCTCGTCCTCGATGGCGTCGGTGTGCGGCGCCTCGCTGTCGCTGATGGACG
CCGGCGTCCCCCTGAAGCGGCCGACCGCGGGCATCGCGATGGGACTGATCCTGGAAGGCGAGCGCTACGCCGTGCTGTCC
GACATCCTCGGCGACGAGGACCATCTCGGCGACATGGACTTCAAGGTGGCGGGCACGGAAGCGGGCATCACCTCGCTGCA
GATGGACATCAAGATCGCCGGCATCACCGAGGAGATCATGAAGGTCGCGCTCGGCCAGGCCAAGGACGGCCGCATCCATA
TTCTGGGCGAGATGTCCAAGGCGCTCGATCGCGCCCGCGCCGAACTCGGCGAACACGCGCCGCGGATCGAGACCTTCAAG
ATCCCGACCGACAAGATCCGGGAAGTGATCGGCACCGGCGGCAAGGTGATCCGCGAGATCGTCGAGAAAACCGGCGCCAA
GGTCAACATCGAGGACGACGGCACCGTCAAGGTCGCCTCCAGCGACGGCGAATCGATCAAGGCCGCGATCAAATGGATCA
AGTCGATCGCCTCCGATCCCGAGGTCGGCGAGATCTACGAAGGAACGGTGGTCAAGGTGATGGAGTTCGGCGCCTTCGTG
AATTTCTTCGGCGCGAAGGACGGCCTCGTTCACATCAGCCAGCTCGCCGCCGGCCGCGTGCAGAAGACCTCCGACGTCGT
CAAGGAAGGCGCCAAGGTCAAGGTCAAGCTGCTCGGCTTCGACGACCGCGGCAAGACCCGGCTGTCGATGAAGGTGGTCG
ACCAGGAAACCGGCGAGGACCTCGAAGCCAAGCAGAAGGCCGAAGGCGACGCGCCGCGGGAAGCCGCAGGCGAGTAG

Upstream 100 bases:

>100_bases
ATCGCCGGACGCTGCGCGAACCGCCAGCGTCCCGCAATCTTGGCATGGTCTTATGGCATCTGGCGTCCGCCATTCCGGGA
TCCGATGAAAGAAGGATCCG

Downstream 100 bases:

>100_bases
GCATGTAGCGTTTTCGAGCGAAGCATGTCCCCGGGCTTGACCCGGGGATGGAATCCGGTTCGCGTGAAGAAAACGCATCA
CTCAATGTCATGGAGCCTCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MFNQHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLTVDYQEKTYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPVRPLFADGWRNETQVIVTVLSHDMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDE
YVLNPTLDEIVDTQLDLVVAGTADAVLMVESEAKELSEEIMLKAVMFGHRHFQPVIDAIIELAEKAAKEPRELKVIDDTE
IEKEMLGLVEQDLRAAYAIPVKQDRYAAVGKVKEKALAHFFPEGQEPKYDKLRVAGVFKELEAKIVRWNILDTGKRIDGR
DVKTVRNIVAEAGVLPRAHGSALFTRGETQAMVVTTLGTGEDEQYIDSLAGTYKETFLLHYNFPPYSVGETGRLGGAKRR
EIGHGKLAWRAIRPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLSLMDAGVPLKRPTAGIAMGLILEGERYAVLS
DILGDEDHLGDMDFKVAGTEAGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFK
IPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFV
NFFGAKDGLVHISQLAAGRVQKTSDVVKEGAKVKVKLLGFDDRGKTRLSMKVVDQETGEDLEAKQKAEGDAPREAAGE

Sequences:

>Translated_718_residues
MFNQHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLTVDYQEKTYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPVRPLFADGWRNETQVIVTVLSHDMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDE
YVLNPTLDEIVDTQLDLVVAGTADAVLMVESEAKELSEEIMLKAVMFGHRHFQPVIDAIIELAEKAAKEPRELKVIDDTE
IEKEMLGLVEQDLRAAYAIPVKQDRYAAVGKVKEKALAHFFPEGQEPKYDKLRVAGVFKELEAKIVRWNILDTGKRIDGR
DVKTVRNIVAEAGVLPRAHGSALFTRGETQAMVVTTLGTGEDEQYIDSLAGTYKETFLLHYNFPPYSVGETGRLGGAKRR
EIGHGKLAWRAIRPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLSLMDAGVPLKRPTAGIAMGLILEGERYAVLS
DILGDEDHLGDMDFKVAGTEAGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFK
IPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFV
NFFGAKDGLVHISQLAAGRVQKTSDVVKEGAKVKVKLLGFDDRGKTRLSMKVVDQETGEDLEAKQKAEGDAPREAAGE
>Mature_718_residues
MFNQHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLTVDYQEKTYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPVRPLFADGWRNETQVIVTVLSHDMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDE
YVLNPTLDEIVDTQLDLVVAGTADAVLMVESEAKELSEEIMLKAVMFGHRHFQPVIDAIIELAEKAAKEPRELKVIDDTE
IEKEMLGLVEQDLRAAYAIPVKQDRYAAVGKVKEKALAHFFPEGQEPKYDKLRVAGVFKELEAKIVRWNILDTGKRIDGR
DVKTVRNIVAEAGVLPRAHGSALFTRGETQAMVVTTLGTGEDEQYIDSLAGTYKETFLLHYNFPPYSVGETGRLGGAKRR
EIGHGKLAWRAIRPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLSLMDAGVPLKRPTAGIAMGLILEGERYAVLS
DILGDEDHLGDMDFKVAGTEAGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFK
IPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFV
NFFGAKDGLVHISQLAAGRVQKTSDVVKEGAKVKVKLLGFDDRGKTRLSMKVVDQETGEDLEAKQKAEGDAPREAAGE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=718, Percent_Identity=38.0222841225627, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=51.0869565217391, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI21361576, Length=87, Percent_Identity=45.9770114942529, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI145693187, Length=694, Percent_Identity=56.628242074928, Blast_Score=768, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=712, Percent_Identity=33.8483146067416, Blast_Score=357, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI17535281, Length=88, Percent_Identity=47.7272727272727, Blast_Score=79, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320850, Length=109, Percent_Identity=34.8623853211009, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=38.7186629526462, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=38.7186629526462, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=38.7186629526462, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=656, Percent_Identity=38.4146341463415, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI20129977, Length=100, Percent_Identity=44, Blast_Score=79, Evalue=2e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78045; Mature: 78045

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNQHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLT
CCCCEEEEEECCCEEEEEECCCHHHHCCCCEEEECCCCEEEHHHHHCCCCCCCCCEEEEE
VDYQEKTYAAGRIPGGYFKREGRPTEKETLVSRLIDRPVRPLFADGWRNETQVIVTVLSH
EECCCCCEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCHHEEEEEECC
DMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDEYVLNPTLDEIVDTQLDLVVA
CCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCEEECCCHHHHHCCCEEEEEE
GTADAVLMVESEAKELSEEIMLKAVMFGHRHFQPVIDAIIELAEKAAKEPRELKVIDDTE
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
IEKEMLGLVEQDLRAAYAIPVKQDRYAAVGKVKEKALAHFFPEGQEPKYDKLRVAGVFKE
HHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LEAKIVRWNILDTGKRIDGRDVKTVRNIVAEAGVLPRAHGSALFTRGETQAMVVTTLGTG
HHCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYIDSLAGTYKETFLLHYNFPPYSVGETGRLGGAKRREIGHGKLAWRAIRPVLPPHH
CCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGASLSLMDAGVPLKRPTAGIAMGLILEGERYAVLS
CCCEEEHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTEAGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEHHHHH
ALDRARAELGEHAPRIETFKIPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVAS
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFVNFFGAKDGLVHISQLAAGRV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCH
QKTSDVVKEGAKVKVKLLGFDDRGKTRLSMKVVDQETGEDLEAKQKAEGDAPREAAGE
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MFNQHSVEIDWGGRPLKLETGKIARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLT
CCCCEEEEEECCCEEEEEECCCHHHHCCCCEEEECCCCEEEHHHHHCCCCCCCCCEEEEE
VDYQEKTYAAGRIPGGYFKREGRPTEKETLVSRLIDRPVRPLFADGWRNETQVIVTVLSH
EECCCCCEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCHHEEEEEECC
DMENDPDILAMVAASAALTLSGAPFKGPIGAARVGFINDEYVLNPTLDEIVDTQLDLVVA
CCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCEEECCCHHHHHCCCEEEEEE
GTADAVLMVESEAKELSEEIMLKAVMFGHRHFQPVIDAIIELAEKAAKEPRELKVIDDTE
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
IEKEMLGLVEQDLRAAYAIPVKQDRYAAVGKVKEKALAHFFPEGQEPKYDKLRVAGVFKE
HHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LEAKIVRWNILDTGKRIDGRDVKTVRNIVAEAGVLPRAHGSALFTRGETQAMVVTTLGTG
HHCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYIDSLAGTYKETFLLHYNFPPYSVGETGRLGGAKRREIGHGKLAWRAIRPVLPPHH
CCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGASLSLMDAGVPLKRPTAGIAMGLILEGERYAVLS
CCCEEEHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTEAGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEHHHHH
ALDRARAELGEHAPRIETFKIPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVAS
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGESIKAAIKWIKSIASDPEVGEIYEGTVVKVMEFGAFVNFFGAKDGLVHISQLAAGRV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCH
QKTSDVVKEGAKVKVKLLGFDDRGKTRLSMKVVDQETGEDLEAKQKAEGDAPREAAGE
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA