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The map label for this gene is trmB

Identifier: 75674219

GI number: 75674219

Start: 20396

End: 21169

Strand: Direct

Name: trmB

Synonym: Nwi_0020

Alternate gene names: 75674219

Gene position: 20396-21169 (Clockwise)

Preceding gene: 75674218

Following gene: 75674220

Centisome position: 0.6

GC content: 66.41

Gene sequence:

>774_bases
GTGACGGTTGTGGTTTCCGACCATCAGAATCCTCGTCCCCCAGGCGATGATGCTGCCCCGCTTGGCCGCACGGGGAATCG
CGATCGCCCGCCAGGCTCGTTCTTCGGGCGTCGCAAGGGACACAGGCTGCGGCCGCACCAGAACGATCTGGTCGCGCAAC
TGCTGCCGCGCCTCGGTTTCGATCCCGCGAGGATCGGACCGTCCGGCCCGGCCGCGCTGTTCGACCCGCCGGTTGATGAG
ACCCGCATCGAGATCGGTTTCGGCGGCGGCGAACATCTTGTCGCCGAGGCGCTGGCGTTTCCCCGCGCCGGCTTCATCGG
CTGCGAGCCCTACGTCAACGGCATGGCAAAGATCCTCGTGCAGATCGAGGCTCACGCCATCCGCAATATCCGCCTGTTCG
CCGGCGATGCGTCGCAACTGCTGGCATGTCTGCCGCCAGCCTCGCTGGCGCGGATCGATCTGATCCATCCCGATCCGTGG
CCCAAGCGCCGCCACTGGAAACGCCGCTTCGTTCAGGACGCGACCGTTGCGGAGATGGCGCGCGTGCTTCGGCCGGAGGG
CGAATTCCGCTTCGTCAGCGACATCGACGATTATTGCGCATGGACGCTGACGCATCTGGCGCGATCACCGGACTTCCTCT
GGCTCGCCGAACGCTCAGCCGACTGGCAGGATCCGTGGTCCGGTTACACCATGACGCGCTACGGCCGCAAAGCGATGCGC
GAAGGCCGCCGCGCGGCGTATCTGAGGTTCCGACGCGAACGCACCGCCGTATAA

Upstream 100 bases:

>100_bases
ACGGACGCCAAGCTCCGTCGCAGCATCGTCGACATGGTCGAACAGATCGCGGCGAGGGAGCCGTCCGACCAGCGCTAGCA
GCCTCCCTCCAACGTTTGCA

Downstream 100 bases:

>100_bases
AGCGGGAGCTATGGCGTTTTCGGGCGAGTGGATGCTCTGGCATCATCATTCGTCGATCGCCTGAATCGGCTTTTTGGCCT
GCGTCGGCCTTGCCGAGGAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTVVVSDHQNPRPPGDDAAPLGRTGNRDRPPGSFFGRRKGHRLRPHQNDLVAQLLPRLGFDPARIGPSGPAALFDPPVDE
TRIEIGFGGGEHLVAEALAFPRAGFIGCEPYVNGMAKILVQIEAHAIRNIRLFAGDASQLLACLPPASLARIDLIHPDPW
PKRRHWKRRFVQDATVAEMARVLRPEGEFRFVSDIDDYCAWTLTHLARSPDFLWLAERSADWQDPWSGYTMTRYGRKAMR
EGRRAAYLRFRRERTAV

Sequences:

>Translated_257_residues
MTVVVSDHQNPRPPGDDAAPLGRTGNRDRPPGSFFGRRKGHRLRPHQNDLVAQLLPRLGFDPARIGPSGPAALFDPPVDE
TRIEIGFGGGEHLVAEALAFPRAGFIGCEPYVNGMAKILVQIEAHAIRNIRLFAGDASQLLACLPPASLARIDLIHPDPW
PKRRHWKRRFVQDATVAEMARVLRPEGEFRFVSDIDDYCAWTLTHLARSPDFLWLAERSADWQDPWSGYTMTRYGRKAMR
EGRRAAYLRFRRERTAV
>Mature_256_residues
TVVVSDHQNPRPPGDDAAPLGRTGNRDRPPGSFFGRRKGHRLRPHQNDLVAQLLPRLGFDPARIGPSGPAALFDPPVDET
RIEIGFGGGEHLVAEALAFPRAGFIGCEPYVNGMAKILVQIEAHAIRNIRLFAGDASQLLACLPPASLARIDLIHPDPWP
KRRHWKRRFVQDATVAEMARVLRPEGEFRFVSDIDDYCAWTLTHLARSPDFLWLAERSADWQDPWSGYTMTRYGRKAMRE
GRRAAYLRFRRERTAV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=227, Percent_Identity=29.5154185022026, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_NITWN (Q3SWQ3)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_316640.1
- ProteinModelPortal:   Q3SWQ3
- SMR:   Q3SWQ3
- STRING:   Q3SWQ3
- GeneID:   3676460
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0020
- NMPDR:   fig|323098.3.peg.18
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   RYGRKAE
- PhylomeDB:   Q3SWQ3
- ProtClustDB:   PRK00121
- BioCyc:   NWIN323098:NWI_0020-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 29020; Mature: 28889

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: BINDING 84-84 BINDING 109-109 BINDING 136-136 BINDING 158-158 BINDING 162-162 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVVSDHQNPRPPGDDAAPLGRTGNRDRPPGSFFGRRKGHRLRPHQNDLVAQLLPRLGF
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCC
DPARIGPSGPAALFDPPVDETRIEIGFGGGEHLVAEALAFPRAGFIGCEPYVNGMAKILV
CHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHH
QIEAHAIRNIRLFAGDASQLLACLPPASLARIDLIHPDPWPKRRHWKRRFVQDATVAEMA
HHHHHHHCEEEEEECCHHHHHHHCCCCHHHEEEEECCCCCCHHHHHHHHHHCCHHHHHHH
RVLRPEGEFRFVSDIDDYCAWTLTHLARSPDFLWLAERSADWQDPWSGYTMTRYGRKAMR
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
EGRRAAYLRFRRERTAV
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVVVSDHQNPRPPGDDAAPLGRTGNRDRPPGSFFGRRKGHRLRPHQNDLVAQLLPRLGF
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCC
DPARIGPSGPAALFDPPVDETRIEIGFGGGEHLVAEALAFPRAGFIGCEPYVNGMAKILV
CHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHH
QIEAHAIRNIRLFAGDASQLLACLPPASLARIDLIHPDPWPKRRHWKRRFVQDATVAEMA
HHHHHHHCEEEEEECCHHHHHHHCCCCHHHEEEEECCCCCCHHHHHHHHHHCCHHHHHHH
RVLRPEGEFRFVSDIDDYCAWTLTHLARSPDFLWLAERSADWQDPWSGYTMTRYGRKAMR
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
EGRRAAYLRFRRERTAV
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA