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The map label for this gene is livK [C]

Identifier: 73539487

GI number: 73539487

Start: 2495383

End: 2496525

Strand: Direct

Name: livK [C]

Synonym: Reut_B5665

Alternate gene names: 73539487

Gene position: 2495383-2496525 (Clockwise)

Preceding gene: 73539486

Following gene: 73539494

Centisome position: 91.53

GC content: 61.77

Gene sequence:

>1143_bases
ATGAAGAGACACCTGCTTGGCATGGCCGCGTTGGCGGCACTTGGCCTGGCTTTCGCCGATTCGGCCATCGCGCAGGTCCA
GGAGGTCAGGCTCGGCTTTGCGGCGCCGATGACCGGCAACCAGGCCCAGTATGGAAAGGATATGCAGAATGGCGTCACCC
TGGCACTCGAGGACTTCAATGCCACCCGACCGCGCATCAACGGGCAGGAGGTCAGGTTCGTACTGGTCTCGGAAGATGAT
CAGGCGGATCCGAAGGCGGGGTCGGTGGTCGCCCAGAAGTTGGTAGACGGCGGTATCAAGGGAATGCTCGGACACTTCAA
TTCGGGGACGAGCATTCCGGCATCCGGCGTGTATAGCCGCGCCGGCATCCCGCAGATCGCCATGGCGACTGCCCCGGAGT
ACACCCGGCAGGGGTTCAAGACGACCTTCCGGATGATGACGTCCGACACGCAGCAGGGAAGCGAGGTCGGTGCGTATGTG
GTCAATAAGCTCGGGGCAAAGCGCATCGCGATTGTCGATGACCGAACGGCCTATGGCCAGGGCCTGGCTGACGAGTTCGA
GAAGGCTGCGAAAGCCGCCGGCGGGACCATCGTTCGACGCGAGTTTACCAACGACAAGGCCGTGGACTTCAAGGCCGTGC
TGACCAATCTGAAACACCAGTCTCCCGATGTGGTGTTCTATGGCGGCGCTGAAGCGCAATCTGCGCCGCTGGCCAGGCAG
ATGCGGGAACTAGGCATGAAATCCGTCCTCGTGTCGGGCGAGATGTCGAAGACGGAGAACTTCATCAAGCTCGCCGGGAA
CGCCGCAGATGGCACCTTGTGCTCGCTGGCGGGTCTGCCGCTCGATCAGATGCCCGGCGGTGCGGGATACAAGCAGCGCT
ACGAGAAGCGCTTCTCGGGCCCGGTGCAGACCTACTCGCCCTATGCATACGATGGTGCCATGGCGATGATGAAGGCAATG
GTGCAGGCGAACTCGTCTGACCCGCAGAAGTATCTGGCTTCGCTCGCTGCAAGCGCCACGCCAGGCGTGACAGCCAGGCA
GATCGCCTATGATGCGCGCGGCGATCTGAAGGAAGGCGGGATTACGATCTACAAGGTCGTATCCGGAAAGTGGCAGCCTG
TCGAAAGCCTTGCCGGGAAATGA

Upstream 100 bases:

>100_bases
TATGTCGTTGCCGCGGCCGCAGTGAGCTTCACGGGTGTGACGCTCGGCTTCCGCGATCGCAGCAAACCAGGCCTGTAACA
ACAACATCGTGAGGATACCT

Downstream 100 bases:

>100_bases
CGACACGCAGATGACAAAAGCCGGACATAGTCCGGCTTTCTTTCCTTATGGACGGTGGAGTTGGCAATCAGTCGTATCCG
GGCCGAGCCAGCCTGATGGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKRHLLGMAALAALGLAFADSAIAQVQEVRLGFAAPMTGNQAQYGKDMQNGVTLALEDFNATRPRINGQEVRFVLVSEDD
QADPKAGSVVAQKLVDGGIKGMLGHFNSGTSIPASGVYSRAGIPQIAMATAPEYTRQGFKTTFRMMTSDTQQGSEVGAYV
VNKLGAKRIAIVDDRTAYGQGLADEFEKAAKAAGGTIVRREFTNDKAVDFKAVLTNLKHQSPDVVFYGGAEAQSAPLARQ
MRELGMKSVLVSGEMSKTENFIKLAGNAADGTLCSLAGLPLDQMPGGAGYKQRYEKRFSGPVQTYSPYAYDGAMAMMKAM
VQANSSDPQKYLASLAASATPGVTARQIAYDARGDLKEGGITIYKVVSGKWQPVESLAGK

Sequences:

>Translated_380_residues
MKRHLLGMAALAALGLAFADSAIAQVQEVRLGFAAPMTGNQAQYGKDMQNGVTLALEDFNATRPRINGQEVRFVLVSEDD
QADPKAGSVVAQKLVDGGIKGMLGHFNSGTSIPASGVYSRAGIPQIAMATAPEYTRQGFKTTFRMMTSDTQQGSEVGAYV
VNKLGAKRIAIVDDRTAYGQGLADEFEKAAKAAGGTIVRREFTNDKAVDFKAVLTNLKHQSPDVVFYGGAEAQSAPLARQ
MRELGMKSVLVSGEMSKTENFIKLAGNAADGTLCSLAGLPLDQMPGGAGYKQRYEKRFSGPVQTYSPYAYDGAMAMMKAM
VQANSSDPQKYLASLAASATPGVTARQIAYDARGDLKEGGITIYKVVSGKWQPVESLAGK
>Mature_380_residues
MKRHLLGMAALAALGLAFADSAIAQVQEVRLGFAAPMTGNQAQYGKDMQNGVTLALEDFNATRPRINGQEVRFVLVSEDD
QADPKAGSVVAQKLVDGGIKGMLGHFNSGTSIPASGVYSRAGIPQIAMATAPEYTRQGFKTTFRMMTSDTQQGSEVGAYV
VNKLGAKRIAIVDDRTAYGQGLADEFEKAAKAAGGTIVRREFTNDKAVDFKAVLTNLKHQSPDVVFYGGAEAQSAPLARQ
MRELGMKSVLVSGEMSKTENFIKLAGNAADGTLCSLAGLPLDQMPGGAGYKQRYEKRFSGPVQTYSPYAYDGAMAMMKAM
VQANSSDPQKYLASLAASATPGVTARQIAYDARGDLKEGGITIYKVVSGKWQPVESLAGK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=362, Percent_Identity=31.2154696132597, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI48994941, Length=356, Percent_Identity=30.3370786516854, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40335; Mature: 40335

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHLLGMAALAALGLAFADSAIAQVQEVRLGFAAPMTGNQAQYGKDMQNGVTLALEDFN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCHHHCCEEEEEECCC
ATRPRINGQEVRFVLVSEDDQADPKAGSVVAQKLVDGGIKGMLGHFNSGTSIPASGVYSR
CCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHH
AGIPQIAMATAPEYTRQGFKTTFRMMTSDTQQGSEVGAYVVNKLGAKRIAIVDDRTAYGQ
CCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHCC
GLADEFEKAAKAAGGTIVRREFTNDKAVDFKAVLTNLKHQSPDVVFYGGAEAQSAPLARQ
CHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHH
MRELGMKSVLVSGEMSKTENFIKLAGNAADGTLCSLAGLPLDQMPGGAGYKQRYEKRFSG
HHHCCHHHEEECCCHHHCCCHHEEECCCCCCCHHHHCCCCHHHCCCCCCHHHHHHHHHCC
PVQTYSPYAYDGAMAMMKAMVQANSSDPQKYLASLAASATPGVTARQIAYDARGDLKEGG
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCC
ITIYKVVSGKWQPVESLAGK
EEEEEEECCCCCCHHHHCCC
>Mature Secondary Structure
MKRHLLGMAALAALGLAFADSAIAQVQEVRLGFAAPMTGNQAQYGKDMQNGVTLALEDFN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCHHHCCEEEEEECCC
ATRPRINGQEVRFVLVSEDDQADPKAGSVVAQKLVDGGIKGMLGHFNSGTSIPASGVYSR
CCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHH
AGIPQIAMATAPEYTRQGFKTTFRMMTSDTQQGSEVGAYVVNKLGAKRIAIVDDRTAYGQ
CCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHCC
GLADEFEKAAKAAGGTIVRREFTNDKAVDFKAVLTNLKHQSPDVVFYGGAEAQSAPLARQ
CHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHH
MRELGMKSVLVSGEMSKTENFIKLAGNAADGTLCSLAGLPLDQMPGGAGYKQRYEKRFSG
HHHCCHHHEEECCCHHHCCCHHEEECCCCCCCHHHHCCCCHHHCCCCCCHHHHHHHHHCC
PVQTYSPYAYDGAMAMMKAMVQANSSDPQKYLASLAASATPGVTARQIAYDARGDLKEGG
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCC
ITIYKVVSGKWQPVESLAGK
EEEEEEECCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA