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The map label for this gene is proP [H]

Identifier: 73539486

GI number: 73539486

Start: 2494065

End: 2495360

Strand: Direct

Name: proP [H]

Synonym: Reut_B5664

Alternate gene names: 73539486

Gene position: 2494065-2495360 (Clockwise)

Preceding gene: 73539482

Following gene: 73539487

Centisome position: 91.49

GC content: 59.57

Gene sequence:

>1296_bases
ATGGAGATCGCAGCAACAATGGAGGCTTCGCAGGATGAAGCCAAGCGGCGGCGGCGAGCAATTACTGCCACCATTCTGGG
AAACGGATTCGAATGGTTTGACTTCATGGTGTATGGCTTCTTTGCCATCACTATCGGGCAACTGTTCTTTCCGACTGGCA
ACGAACTGACCTCGTTGCTGCTGTCTGTGGCTACCTTCGGCGTGGGTTTTGTGGTGCGCCCGGTCGGCGGCATCCTGATC
GGGATGTACTCGGATCGGGTCGGACGCAAGGCCGCCTTGTCTTTGACCATTCTGCTGATGGCTGGCGGAACGGCATTGAT
CGGCATCGCGCCCACTTATGAACAGATCGGCATTGGGGCGCCGATCCTGATCATCATCGCGCGACTGATGCAGGGGTTTT
CGGCCGGTGGCGAAATGGGAAGTGCGACCGCATTCCTGACAGAGCATGCGCCTGCAAACCAGAAGGGCTACTACGCGAGC
TGGATCCAGAGCAGCATCGGCTTTGCCGTCGTGCTTGGCGCTGCAGCCGGCACCGTGCTTACCCTGTGGCTCGCGCCGGA
AGAGGTCAAGGCATGGGGATGGCGCATTCCATTCCTGCTCGGCATCCTCATCGGGCCCATTGGCTTCTACATCCGAAGCA
ATGTCGACGAGACGCCCGCGTTCCTGGCTACGACGGACAAAGTAAAGAAGCCGTTCAGCGAGGTGCTCTCGAACTTTCCG
CGCGAGACCTTCACGACGTTCTCCATGGTCATCCTGTGGACGGTTTGCATCTATGTGCTGCTGTTCTACATGCCGACGTA
CGCCGTCAAGACGCTGAAGATGCCGCAATCGATGTCGTTCATCGCCGGTGTGGTGGGGGGCCTCATCATCACGGTGCTGT
CGCCGTTTGTGGGCGCACTGTCTGACCGGATGGGTCGCAAGAAGATTCTGGCGCTGAGTTCCGGCGGCATCCTGCTGCTT
GCGTACCCAATGTTTGCGCTCGTGGTATCCGCTCCTACGCAAACCAGCCTGATCATCTTCCAGTCGGTTTTCGGCATTCT
CATTGCCGGCTACACCGGTCCCATCCTGGCCGCGTTCTCCGAGATCTTCCCGACGCGGGTGCTGTCGACGGGGCTCTCGG
TTGCCTACAACGTCGCGGTCATGACTTTTGGCGGGTTCGCCTCGTTGATCCTGACATGGCTGATCGCCACCACAGGTAGT
GCCATGGCGCCCGCCTTGTATGTCGTTGCCGCGGCCGCAGTGAGCTTCACGGGTGTGACGCTCGGCTTCCGCGATCGCAG
CAAACCAGGCCTGTAA

Upstream 100 bases:

>100_bases
CCTGGGATTGAATGGCCAGTAGCTCGAGTTGAGCAAGAACGAGTGTGCCGACACAAGCATCGGATTACACACTTACCCGG
CCAAGACAAAGGAGTGCGAG

Downstream 100 bases:

>100_bases
CAACAACATCGTGAGGATACCTATGAAGAGACACCTGCTTGGCATGGCCGCGTTGGCGGCACTTGGCCTGGCTTTCGCCG
ATTCGGCCATCGCGCAGGTC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MEIAATMEASQDEAKRRRRAITATILGNGFEWFDFMVYGFFAITIGQLFFPTGNELTSLLLSVATFGVGFVVRPVGGILI
GMYSDRVGRKAALSLTILLMAGGTALIGIAPTYEQIGIGAPILIIIARLMQGFSAGGEMGSATAFLTEHAPANQKGYYAS
WIQSSIGFAVVLGAAAGTVLTLWLAPEEVKAWGWRIPFLLGILIGPIGFYIRSNVDETPAFLATTDKVKKPFSEVLSNFP
RETFTTFSMVILWTVCIYVLLFYMPTYAVKTLKMPQSMSFIAGVVGGLIITVLSPFVGALSDRMGRKKILALSSGGILLL
AYPMFALVVSAPTQTSLIIFQSVFGILIAGYTGPILAAFSEIFPTRVLSTGLSVAYNVAVMTFGGFASLILTWLIATTGS
AMAPALYVVAAAAVSFTGVTLGFRDRSKPGL

Sequences:

>Translated_431_residues
MEIAATMEASQDEAKRRRRAITATILGNGFEWFDFMVYGFFAITIGQLFFPTGNELTSLLLSVATFGVGFVVRPVGGILI
GMYSDRVGRKAALSLTILLMAGGTALIGIAPTYEQIGIGAPILIIIARLMQGFSAGGEMGSATAFLTEHAPANQKGYYAS
WIQSSIGFAVVLGAAAGTVLTLWLAPEEVKAWGWRIPFLLGILIGPIGFYIRSNVDETPAFLATTDKVKKPFSEVLSNFP
RETFTTFSMVILWTVCIYVLLFYMPTYAVKTLKMPQSMSFIAGVVGGLIITVLSPFVGALSDRMGRKKILALSSGGILLL
AYPMFALVVSAPTQTSLIIFQSVFGILIAGYTGPILAAFSEIFPTRVLSTGLSVAYNVAVMTFGGFASLILTWLIATTGS
AMAPALYVVAAAAVSFTGVTLGFRDRSKPGL
>Mature_431_residues
MEIAATMEASQDEAKRRRRAITATILGNGFEWFDFMVYGFFAITIGQLFFPTGNELTSLLLSVATFGVGFVVRPVGGILI
GMYSDRVGRKAALSLTILLMAGGTALIGIAPTYEQIGIGAPILIIIARLMQGFSAGGEMGSATAFLTEHAPANQKGYYAS
WIQSSIGFAVVLGAAAGTVLTLWLAPEEVKAWGWRIPFLLGILIGPIGFYIRSNVDETPAFLATTDKVKKPFSEVLSNFP
RETFTTFSMVILWTVCIYVLLFYMPTYAVKTLKMPQSMSFIAGVVGGLIITVLSPFVGALSDRMGRKKILALSSGGILLL
AYPMFALVVSAPTQTSLIIFQSVFGILIAGYTGPILAAFSEIFPTRVLSTGLSVAYNVAVMTFGGFASLILTWLIATTGS
AMAPALYVVAAAAVSFTGVTLGFRDRSKPGL

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=35.8139534883721, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1788292, Length=413, Percent_Identity=30.0242130750605, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788942, Length=441, Percent_Identity=31.7460317460317, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789941, Length=440, Percent_Identity=32.7272727272727, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87082404, Length=383, Percent_Identity=26.1096605744125, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082231, Length=203, Percent_Identity=29.5566502463054, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45920; Mature: 45920

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIAATMEASQDEAKRRRRAITATILGNGFEWFDFMVYGFFAITIGQLFFPTGNELTSLL
CCCCHHHCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LSVATFGVGFVVRPVGGILIGMYSDRVGRKAALSLTILLMAGGTALIGIAPTYEQIGIGA
HHHHHHHHHHHHHHHHHEEEEHHHHCCCCHHHHHHEEEEEECCCEEEEECCCHHHHCCCC
PILIIIARLMQGFSAGGEMGSATAFLTEHAPANQKGYYASWIQSSIGFAVVLGAAAGTVL
HHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCEE
TLWLAPEEVKAWGWRIPFLLGILIGPIGFYIRSNVDETPAFLATTDKVKKPFSEVLSNFP
EEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHHHHHHHHHHHHCC
RETFTTFSMVILWTVCIYVLLFYMPTYAVKTLKMPQSMSFIAGVVGGLIITVLSPFVGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SDRMGRKKILALSSGGILLLAYPMFALVVSAPTQTSLIIFQSVFGILIAGYTGPILAAFS
HHHCCCCEEEEEECCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
EIFPTRVLSTGLSVAYNVAVMTFGGFASLILTWLIATTGSAMAPALYVVAAAAVSFTGVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCEE
LGFRDRSKPGL
EECCCCCCCCC
>Mature Secondary Structure
MEIAATMEASQDEAKRRRRAITATILGNGFEWFDFMVYGFFAITIGQLFFPTGNELTSLL
CCCCHHHCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LSVATFGVGFVVRPVGGILIGMYSDRVGRKAALSLTILLMAGGTALIGIAPTYEQIGIGA
HHHHHHHHHHHHHHHHHEEEEHHHHCCCCHHHHHHEEEEEECCCEEEEECCCHHHHCCCC
PILIIIARLMQGFSAGGEMGSATAFLTEHAPANQKGYYASWIQSSIGFAVVLGAAAGTVL
HHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCEE
TLWLAPEEVKAWGWRIPFLLGILIGPIGFYIRSNVDETPAFLATTDKVKKPFSEVLSNFP
EEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHHHHHHHHHHHHCC
RETFTTFSMVILWTVCIYVLLFYMPTYAVKTLKMPQSMSFIAGVVGGLIITVLSPFVGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SDRMGRKKILALSSGGILLLAYPMFALVVSAPTQTSLIIFQSVFGILIAGYTGPILAAFS
HHHCCCCEEEEEECCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
EIFPTRVLSTGLSVAYNVAVMTFGGFASLILTWLIATTGSAMAPALYVVAAAAVSFTGVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCEE
LGFRDRSKPGL
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]