Click here to switch to the map view.

The map label for this gene is dmlR [H]

Identifier: 73539475

GI number: 73539475

Start: 2478233

End: 2479156

Strand: Direct

Name: dmlR [H]

Synonym: Reut_B5653

Alternate gene names: 73539475

Gene position: 2478233-2479156 (Clockwise)

Preceding gene: 73539470

Following gene: 73539476

Centisome position: 90.91

GC content: 61.8

Gene sequence:

>924_bases
ATGGATTCGGACTCGCAGCTCGCCTTTTTCTGCCTGCTCGTGAAGCAGGGGAGCCTTGCCGCCACAGCGCGTGAGCTGAA
TCTGACGCCGCCCGCTGTATCGCGGCGGCTGTCACAACTGGAAGCGCGTCTGGGAGTGCGGCTGCTGAACCGCACGACGC
GACGGATCAGTCTCACTAACGAAGGGGAGGTCTATTTCGCGAACGCGATGCGCATCCTCGGCGATATCGACGAAATGGAG
AGGCTGGTATCGAGCAGTCGCGAGGCGCCGAAAGGCCTGTTGCGTGTCAATGCGCCGCTGGGTTTCGGGCGCTCTTATAT
CGGTCCGGCCATTTCGGGGTTCTCGAAGACCTATCCGGACGTCGAAGTCCAGCTCCAGCTGACGGATCGCCCGATCAGCT
TGCCCGATGAAGCGATCGATGTATCGATCCGCTTCGGCGACCTGCCTGATTCCCGGCTCATCGCCAAGCGGATTGCGCCG
AACCGGCGTCTGCTTTGCGCGTCACCGGCTTATCTGCGCTCTGCGGGCGAACCGCTTTTGCCGCACGACCTGACGCAGCA
TGCATGCATCGTGCTGCGGCAAAACGAGACCGCCTATGGCAACTGGCGGCTGACCCGTGGGAAGCAGACCGAGACCATCA
AGGTTCACGGCAAACTCAGCACCAACGACGGAGAAGTGGCCCTCAACTGGGCGCTGGAAGGACACGGCATCCTGATGCGC
GCCGAGTGGGATGTCGCCAAGTTCTTGCGCAGCGGGCGGTTGGTCCAGGTCCTCACTGACTATGCCACGCCTCCCGCGGA
CATCTACGCTGTCTATCTGGAACGCCTGAACCAGTCTGCCAAGGTCTCTTACTTCGTCGAGCACCTCCGGAGCTTTCTGA
GGCATCACGCCGACAGCCCGAGCAAGGACGGGTCGAATTGGTAG

Upstream 100 bases:

>100_bases
GCTGCTGGTAGCGCGCTTGCTTGGTGTGATCGAAGGTTATTCGCTTCAGTGCGGCGATGAATCGATGTAAAGTTAAGACA
TCCTTGCAAAATTTTTACTA

Downstream 100 bases:

>100_bases
AAATTTAGCAACCGCGAAGGCCGGCTTTTGGGGGGCGGCAATCATGGGTGCACAAGCCGGCCGGGCGCTCGGCACTTGTT
GAATTACAATGCGTGCATGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MDSDSQLAFFCLLVKQGSLAATARELNLTPPAVSRRLSQLEARLGVRLLNRTTRRISLTNEGEVYFANAMRILGDIDEME
RLVSSSREAPKGLLRVNAPLGFGRSYIGPAISGFSKTYPDVEVQLQLTDRPISLPDEAIDVSIRFGDLPDSRLIAKRIAP
NRRLLCASPAYLRSAGEPLLPHDLTQHACIVLRQNETAYGNWRLTRGKQTETIKVHGKLSTNDGEVALNWALEGHGILMR
AEWDVAKFLRSGRLVQVLTDYATPPADIYAVYLERLNQSAKVSYFVEHLRSFLRHHADSPSKDGSNW

Sequences:

>Translated_307_residues
MDSDSQLAFFCLLVKQGSLAATARELNLTPPAVSRRLSQLEARLGVRLLNRTTRRISLTNEGEVYFANAMRILGDIDEME
RLVSSSREAPKGLLRVNAPLGFGRSYIGPAISGFSKTYPDVEVQLQLTDRPISLPDEAIDVSIRFGDLPDSRLIAKRIAP
NRRLLCASPAYLRSAGEPLLPHDLTQHACIVLRQNETAYGNWRLTRGKQTETIKVHGKLSTNDGEVALNWALEGHGILMR
AEWDVAKFLRSGRLVQVLTDYATPPADIYAVYLERLNQSAKVSYFVEHLRSFLRHHADSPSKDGSNW
>Mature_307_residues
MDSDSQLAFFCLLVKQGSLAATARELNLTPPAVSRRLSQLEARLGVRLLNRTTRRISLTNEGEVYFANAMRILGDIDEME
RLVSSSREAPKGLLRVNAPLGFGRSYIGPAISGFSKTYPDVEVQLQLTDRPISLPDEAIDVSIRFGDLPDSRLIAKRIAP
NRRLLCASPAYLRSAGEPLLPHDLTQHACIVLRQNETAYGNWRLTRGKQTETIKVHGKLSTNDGEVALNWALEGHGILMR
AEWDVAKFLRSGRLVQVLTDYATPPADIYAVYLERLNQSAKVSYFVEHLRSFLRHHADSPSKDGSNW

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=41.0958904109589, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=39.3103448275862, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=33.2214765100671, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=31.438127090301, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=29.5138888888889, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1789639, Length=310, Percent_Identity=28.3870967741935, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=26.3888888888889, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=24.3986254295533, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789173, Length=269, Percent_Identity=25.6505576208178, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=178, Percent_Identity=31.4606741573034, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790011, Length=302, Percent_Identity=22.1854304635762, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786448, Length=246, Percent_Identity=23.9837398373984, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790262, Length=244, Percent_Identity=25, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=120, Percent_Identity=26.6666666666667, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34294; Mature: 34294

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSDSQLAFFCLLVKQGSLAATARELNLTPPAVSRRLSQLEARLGVRLLNRTTRRISLTN
CCCCCCEEEEEEEECCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEECC
EGEVYFANAMRILGDIDEMERLVSSSREAPKGLLRVNAPLGFGRSYIGPAISGFSKTYPD
CCCEEEHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHCHHHHCHHCCCCC
VEVQLQLTDRPISLPDEAIDVSIRFGDLPDSRLIAKRIAPNRRLLCASPAYLRSAGEPLL
EEEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEECCCHHHHCCCCCCC
PHDLTQHACIVLRQNETAYGNWRLTRGKQTETIKVHGKLSTNDGEVALNWALEGHGILMR
CCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEEEEECCCCCEEEEEEECCCEEEEE
AEWDVAKFLRSGRLVQVLTDYATPPADIYAVYLERLNQSAKVSYFVEHLRSFLRHHADSP
ECHHHHHHHHCCCEEEEHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
SKDGSNW
CCCCCCC
>Mature Secondary Structure
MDSDSQLAFFCLLVKQGSLAATARELNLTPPAVSRRLSQLEARLGVRLLNRTTRRISLTN
CCCCCCEEEEEEEECCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEECC
EGEVYFANAMRILGDIDEMERLVSSSREAPKGLLRVNAPLGFGRSYIGPAISGFSKTYPD
CCCEEEHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHCHHHHCHHCCCCC
VEVQLQLTDRPISLPDEAIDVSIRFGDLPDSRLIAKRIAPNRRLLCASPAYLRSAGEPLL
EEEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEECCCHHHHCCCCCCC
PHDLTQHACIVLRQNETAYGNWRLTRGKQTETIKVHGKLSTNDGEVALNWALEGHGILMR
CCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEEEEECCCCCEEEEEEECCCEEEEE
AEWDVAKFLRSGRLVQVLTDYATPPADIYAVYLERLNQSAKVSYFVEHLRSFLRHHADSP
ECHHHHHHHHCCCEEEEHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
SKDGSNW
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]