Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 73539355

GI number: 73539355

Start: 2333357

End: 2334265

Strand: Reverse

Name: gcvA [H]

Synonym: Reut_B5533

Alternate gene names: 73539355

Gene position: 2334265-2333357 (Counterclockwise)

Preceding gene: 73539357

Following gene: 73539353

Centisome position: 85.62

GC content: 68.32

Gene sequence:

>909_bases
ATGAGAAACGACATCCCCAACCTTGGCGCGCTGCAGGCCTTCGAGGCTTCGGCGCGGCTCGGCAGCTTCACGCGTGCGGC
CGACGAACTGGCGCTTACGCAAAGCGCCGTGGGCCGGCAGGTGGCGATGCTGGAGCAACGGCTGGGCGTGGCGCTGTTCT
CGCGCGTGCGCCGGCGCCTGGTGCTGACCGACACGGGCCGCGAGTATGCGGCCCGCATCCGCCGCCATCTGGATCATCTT
CAGCGCGATACGCTCGAAATCACCGCTGGCCGCGTCATGGGTTTCACGCTGGAACTCGCAGTCGTGCCCACCTTTGCCAC
GCAATGGCTGATTCCACGCCTGCCCGACTTTGCGCGGCGGCACCCTAACATCACCGTCAACCTGAGCGCCCGCTCGCAGC
CGTTCTCGTTCCAGGAGTGCGCTTACGACGCCGCGATCTACTTCGGCGACCAGTTCTGGCCCGGCACGCGCGGCGGCATG
ATTTTCCGCGAAGGCGGCGTGGTGCCGGTCTGCAGCCCGGCGTTCCTTGAGAAGAACCCGGTCGCTGATGCCGCCACGTT
CTCGCGTTGCCGCCACCTGCACCTCAGCACGCGCGCGCAGGCATGGCGCGACTGGTATGCCGTGCAGGGCTGGGACTACA
GCATCGCCGCATCGCGCGGGCCGCGCTATGAGCTGTTCACGATGATGACCGCCGCCGCCGCGGCCGGCCTCGGCATCGGC
CTGGCGCCGCGGCTGCTGGTCGAGCGCGAACTGCGCAGCGGCGAACTCGTCGTGCCGGTCGAGCGCGTGCTGGATGAACA
GCAGGGCTACTACTTTGCCTATCCCGAGGGCCGGCCGCCGTCGGAAGCACTCGAGGCGTTCCGCGAATGGGTCCTGGAGC
TGCCGCGCGAAACGACTTCGGCGACGTAA

Upstream 100 bases:

>100_bases
AAGCGCGGAATCCGCACAACATTGTGCGTGCCGCGCACGAAGTGCCGCCCCGGCAGCGGGCGCTATACTGAGCGCCCCCG
CGCCGTCCGCAGTCATTCCC

Downstream 100 bases:

>100_bases
GCGCCCTCAGCCGAGCTGCGCCGTGCGCTGCTCCAACGCGCGGATCACGGCCGCCATGTCATCCTGGCCGAGTCCCAGCG
CTTCGGTCTCGCCGTACAGC

Product: regulatory protein LysR

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRNDIPNLGALQAFEASARLGSFTRAADELALTQSAVGRQVAMLEQRLGVALFSRVRRRLVLTDTGREYAARIRRHLDHL
QRDTLEITAGRVMGFTLELAVVPTFATQWLIPRLPDFARRHPNITVNLSARSQPFSFQECAYDAAIYFGDQFWPGTRGGM
IFREGGVVPVCSPAFLEKNPVADAATFSRCRHLHLSTRAQAWRDWYAVQGWDYSIAASRGPRYELFTMMTAAAAAGLGIG
LAPRLLVERELRSGELVVPVERVLDEQQGYYFAYPEGRPPSEALEAFREWVLELPRETTSAT

Sequences:

>Translated_302_residues
MRNDIPNLGALQAFEASARLGSFTRAADELALTQSAVGRQVAMLEQRLGVALFSRVRRRLVLTDTGREYAARIRRHLDHL
QRDTLEITAGRVMGFTLELAVVPTFATQWLIPRLPDFARRHPNITVNLSARSQPFSFQECAYDAAIYFGDQFWPGTRGGM
IFREGGVVPVCSPAFLEKNPVADAATFSRCRHLHLSTRAQAWRDWYAVQGWDYSIAASRGPRYELFTMMTAAAAAGLGIG
LAPRLLVERELRSGELVVPVERVLDEQQGYYFAYPEGRPPSEALEAFREWVLELPRETTSAT
>Mature_302_residues
MRNDIPNLGALQAFEASARLGSFTRAADELALTQSAVGRQVAMLEQRLGVALFSRVRRRLVLTDTGREYAARIRRHLDHL
QRDTLEITAGRVMGFTLELAVVPTFATQWLIPRLPDFARRHPNITVNLSARSQPFSFQECAYDAAIYFGDQFWPGTRGGM
IFREGGVVPVCSPAFLEKNPVADAATFSRCRHLHLSTRAQAWRDWYAVQGWDYSIAASRGPRYELFTMMTAAAAAGLGIG
LAPRLLVERELRSGELVVPVERVLDEQQGYYFAYPEGRPPSEALEAFREWVLELPRETTSAT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=34.2372881355932, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=33.1034482758621, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788706, Length=312, Percent_Identity=31.0897435897436, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693193, Length=307, Percent_Identity=28.0130293159609, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI157672245, Length=126, Percent_Identity=41.2698412698413, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=25.3424657534247, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=24.8275862068966, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=26.4406779661017, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33878; Mature: 33878

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNDIPNLGALQAFEASARLGSFTRAADELALTQSAVGRQVAMLEQRLGVALFSRVRRRL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VLTDTGREYAARIRRHLDHLQRDTLEITAGRVMGFTLELAVVPTFATQWLIPRLPDFARR
EEECCCHHHHHHHHHHHHHHHHHHHEEECCEEEEEEEEEEEEHHHHHHHHHCCCCHHHHH
HPNITVNLSARSQPFSFQECAYDAAIYFGDQFWPGTRGGMIFREGGVVPVCSPAFLEKNP
CCCEEEEECCCCCCCCHHHHHHHHHEEECCCCCCCCCCCEEEECCCCCEECCHHHHCCCC
VADAATFSRCRHLHLSTRAQAWRDWYAVQGWDYSIAASRGPRYELFTMMTAAAAAGLGIG
CCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
LAPRLLVERELRSGELVVPVERVLDEQQGYYFAYPEGRPPSEALEAFREWVLELPRETTS
CHHHHHHHHHHCCCCEEEEHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
AT
CC
>Mature Secondary Structure
MRNDIPNLGALQAFEASARLGSFTRAADELALTQSAVGRQVAMLEQRLGVALFSRVRRRL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VLTDTGREYAARIRRHLDHLQRDTLEITAGRVMGFTLELAVVPTFATQWLIPRLPDFARR
EEECCCHHHHHHHHHHHHHHHHHHHEEECCEEEEEEEEEEEEHHHHHHHHHCCCCHHHHH
HPNITVNLSARSQPFSFQECAYDAAIYFGDQFWPGTRGGMIFREGGVVPVCSPAFLEKNP
CCCEEEEECCCCCCCCHHHHHHHHHEEECCCCCCCCCCCEEEECCCCCEECCHHHHCCCC
VADAATFSRCRHLHLSTRAQAWRDWYAVQGWDYSIAASRGPRYELFTMMTAAAAAGLGIG
CCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
LAPRLLVERELRSGELVVPVERVLDEQQGYYFAYPEGRPPSEALEAFREWVLELPRETTS
CHHHHHHHHHHCCCCEEEEHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
AT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]