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The map label for this gene is gbpR [H]

Identifier: 73539319

GI number: 73539319

Start: 2294074

End: 2295021

Strand: Reverse

Name: gbpR [H]

Synonym: Reut_B5497

Alternate gene names: 73539319

Gene position: 2295021-2294074 (Counterclockwise)

Preceding gene: 73539326

Following gene: 73539317

Centisome position: 84.19

GC content: 68.14

Gene sequence:

>948_bases
ATGTCCCTGAATCCCACCTCGCTTGGCCGGAAATTGCGCTTCGCGCAGATCCTCGTGTTCGAACAGGTGCTGCGCAGCGG
CTCGATCCTGCGCGCAGCGCAGGCGCTCAACATGACGCAGCCGGCGGTCACGAAGGTGATCCACGAACTGGAAAGCCACC
TCGGCGCGCCACTGCTGATCCGCGGCAACCGCGGCGTGACCACGACCGAGTTCGGCGAGCTCGTCGCGCGGCGCGCCAAG
ACCCTGCTGGCCGAGCTGCGCCACCTGACTGACGAGATCAGCGCGTTCCGCGAAGGCACGTCGGGCCAGGTGCTGATCGG
CACGCTGATCTCGGCGTCGGCGGTGCTGCTGCCACGCGCCATCCAATTGCTGAAGGAACAGGCACCGGCGGTGTCGGTGA
CCTTGCGCGTCGGCCAGATGGACCAGCTGCTGCCCGCGCTGGCGGTCGGCGACCTCGACGTGGTTGTCGGGCGCATTCCG
GAAGATGTGTCGTGGCGGGCCACCGCACCCGGGCTTGAAGCCGAAATCCTGCACCGGGAGCCGCTGTGCGTGGTCGGCGG
CGTGCATCATCCGATGGCATCGCGCGCGTCGACCACCCTGCAGGCGCTGAGCGACTATCCGTGGATCCTGCCCACGCGCG
ACTCGTCGCTGCGGCAGACGGCCGAACGCCTGTTCTACGACACGGGCCTGGCGCTGCCCGCGAACGTCGTGGAGTCGCTG
TCGGTCATGACCAACGTCGCGCTGATGTTCGACCAGAAGACCCTTGGGCTGATGCCGCGCTCGGCCGCGCGGCAATTCGT
CGATACCGGCAGCCTGGCGATGATCCCGCTGGAGCATGCGCCGTCATTCGGCGATGTCGGCTTCTTCGCGGTACCGGGCA
CGCTGGCCGTGCGTGCGGTCGAACTGTTCAAGGATTGCCTGCGGGCGGCCGCGCGCGAAGTCGGCTAG

Upstream 100 bases:

>100_bases
TATTCCTGAAAAATGAAACTTTCGGCTCCTGATATTCCAGTCTAGAATACCGGCCACGCCCGCATTGCGCCGAACCCAGC
AGCGCGCCTCGCCCTTGCCC

Downstream 100 bases:

>100_bases
CACCCCGTCATTCGCACCGCTCGCGCGCCAGCGACTCCCGCGTAAAGCCATGCCGCACGCGCAGCTGGTGCTCGACCGCG
TCGGCCTGGTCGAGGCAGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Galactose-binding protein regulator; GBP regulator [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSLNPTSLGRKLRFAQILVFEQVLRSGSILRAAQALNMTQPAVTKVIHELESHLGAPLLIRGNRGVTTTEFGELVARRAK
TLLAELRHLTDEISAFREGTSGQVLIGTLISASAVLLPRAIQLLKEQAPAVSVTLRVGQMDQLLPALAVGDLDVVVGRIP
EDVSWRATAPGLEAEILHREPLCVVGGVHHPMASRASTTLQALSDYPWILPTRDSSLRQTAERLFYDTGLALPANVVESL
SVMTNVALMFDQKTLGLMPRSAARQFVDTGSLAMIPLEHAPSFGDVGFFAVPGTLAVRAVELFKDCLRAAAREVG

Sequences:

>Translated_315_residues
MSLNPTSLGRKLRFAQILVFEQVLRSGSILRAAQALNMTQPAVTKVIHELESHLGAPLLIRGNRGVTTTEFGELVARRAK
TLLAELRHLTDEISAFREGTSGQVLIGTLISASAVLLPRAIQLLKEQAPAVSVTLRVGQMDQLLPALAVGDLDVVVGRIP
EDVSWRATAPGLEAEILHREPLCVVGGVHHPMASRASTTLQALSDYPWILPTRDSSLRQTAERLFYDTGLALPANVVESL
SVMTNVALMFDQKTLGLMPRSAARQFVDTGSLAMIPLEHAPSFGDVGFFAVPGTLAVRAVELFKDCLRAAAREVG
>Mature_314_residues
SLNPTSLGRKLRFAQILVFEQVLRSGSILRAAQALNMTQPAVTKVIHELESHLGAPLLIRGNRGVTTTEFGELVARRAKT
LLAELRHLTDEISAFREGTSGQVLIGTLISASAVLLPRAIQLLKEQAPAVSVTLRVGQMDQLLPALAVGDLDVVVGRIPE
DVSWRATAPGLEAEILHREPLCVVGGVHHPMASRASTTLQALSDYPWILPTRDSSLRQTAERLFYDTGLALPANVVESLS
VMTNVALMFDQKTLGLMPRSAARQFVDTGSLAMIPLEHAPSFGDVGFFAVPGTLAVRAVELFKDCLRAAAREVG

Specific function: Activator of the expression of chvE when bound to its inducer and represses its expression in the absence of inducer (L- arabinose, D-fucose or D-galactose). Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789506, Length=271, Percent_Identity=29.8892988929889, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787601, Length=271, Percent_Identity=29.520295202952, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87081904, Length=273, Percent_Identity=27.1062271062271, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI157672245, Length=261, Percent_Identity=26.4367816091954, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790399, Length=199, Percent_Identity=29.1457286432161, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788297, Length=256, Percent_Identity=26.5625, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=212, Percent_Identity=30.188679245283, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788887, Length=190, Percent_Identity=28.9473684210526, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33937; Mature: 33806

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNPTSLGRKLRFAQILVFEQVLRSGSILRAAQALNMTQPAVTKVIHELESHLGAPLLI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEE
RGNRGVTTTEFGELVARRAKTLLAELRHLTDEISAFREGTSGQVLIGTLISASAVLLPRA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IQLLKEQAPAVSVTLRVGQMDQLLPALAVGDLDVVVGRIPEDVSWRATAPGLEAEILHRE
HHHHHHCCCEEEEEEEECCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHCCC
PLCVVGGVHHPMASRASTTLQALSDYPWILPTRDSSLRQTAERLFYDTGLALPANVVESL
CEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHH
SVMTNVALMFDQKTLGLMPRSAARQFVDTGSLAMIPLEHAPSFGDVGFFAVPGTLAVRAV
HHHHHHHHHCCCHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHH
ELFKDCLRAAAREVG
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLNPTSLGRKLRFAQILVFEQVLRSGSILRAAQALNMTQPAVTKVIHELESHLGAPLLI
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEE
RGNRGVTTTEFGELVARRAKTLLAELRHLTDEISAFREGTSGQVLIGTLISASAVLLPRA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IQLLKEQAPAVSVTLRVGQMDQLLPALAVGDLDVVVGRIPEDVSWRATAPGLEAEILHRE
HHHHHHCCCEEEEEEEECCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHCCC
PLCVVGGVHHPMASRASTTLQALSDYPWILPTRDSSLRQTAERLFYDTGLALPANVVESL
CEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHH
SVMTNVALMFDQKTLGLMPRSAARQFVDTGSLAMIPLEHAPSFGDVGFFAVPGTLAVRAV
HHHHHHHHHCCCHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHH
ELFKDCLRAAAREVG
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253677; 2156804 [H]