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The map label for this gene is aes [H]

Identifier: 73539284

GI number: 73539284

Start: 2253292

End: 2254227

Strand: Direct

Name: aes [H]

Synonym: Reut_B5462

Alternate gene names: 73539284

Gene position: 2253292-2254227 (Clockwise)

Preceding gene: 73539283

Following gene: 73539285

Centisome position: 82.65

GC content: 68.8

Gene sequence:

>936_bases
ATGGCGCTCGACGAGCAGGCCCGCTACATCCTTGACATGATTGCGTCGGCAGGACGGCCGCCATTGCATACCTGCACGCC
GGAGCAGGCGCGGCAGTCGTTCGATTTCCGGCCACTGGCCGGCGTGCCGGACGCCGTGGCTCAGATCGAGGAAGTCACGA
TTCCCGGCCCCGCCGGCGATATCCGCGTCCGCATCTACACCCCCGACGGTGAAGGCCCGTTTCCGGCGCTGGTGTATTGC
CACGGTGGTGGCTGGGTCGTCGGTGACCTCGACACGGTAGATGTGCCATGCCGTCGCCTCGCTACCCGCGCGTCTTGCGT
GGTCGTGTCGGTCGACTACCGGCTGGCACCCGAACACCGGTTTCCCGCCGCCACTGAGGACGCTTACGCCGCGTTCCAGT
GGCTGGTGAGCAATGCGCGCGCGCAGCAGGTCGACGCCACACGTATCGCCGTGGGTGGCGACAGCGCCGGTGGCAACCTC
GCTGCAGCCGTCGCGTTGATGGCGCGCGACCGCGCCGCGCCGCAGCCGTGCTTCCAGGTGTTGCTCTACCCGGTGACCGA
CGGCACGCTCGACACGCCGTCGTACCGCGAGAACGCCGAGGGCTACCTGCTCACGCGCGACAGCATGGTCTGGTTCTGGA
ACCACTACGTGGGCGATGCCGACCGCACGCATCCCTACGCCTCGCCATTGCGCGCCGATCACCATCGCGGCCTGCCGCCG
GCCTTCGTGGTCACGGCGGAGTTCGATCCGCTGCGCGACGAGGGCGAGGCCTACGCGCGCCGGCTTGCAGAGGCCGGCAC
GCCGGTGGAATGCAAGCGGTATGACGGCACCATCCACGGCTTCTGCTGGATGCCGGGCGTACTCGACAAGGGCCGGCAGG
CACTCGACGACACGGCAGCCGCACTCGCGGCGGCGTTCCAGAGCGACAACCGATAA

Upstream 100 bases:

>100_bases
CGTCTATCGCGAGCTGTGCGCTCGCGTGGCGGCGAACGGGTACGACGGCTTCCTGCAGGCGTAAGCCGGGCCACAACAAC
TACGACGGAGACAAGAGACC

Downstream 100 bases:

>100_bases
GAGGTACTGACATGCGACTGCAAGGCAAGGTTGCGATTCTCACCGGTGCTGCGGGCGGTATCGGCCGCGCTACCGCGCTT
GGTTTCGCGCGCGAAGGCGC

Product: esterase/lipase/thioesterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MALDEQARYILDMIASAGRPPLHTCTPEQARQSFDFRPLAGVPDAVAQIEEVTIPGPAGDIRVRIYTPDGEGPFPALVYC
HGGGWVVGDLDTVDVPCRRLATRASCVVVSVDYRLAPEHRFPAATEDAYAAFQWLVSNARAQQVDATRIAVGGDSAGGNL
AAAVALMARDRAAPQPCFQVLLYPVTDGTLDTPSYRENAEGYLLTRDSMVWFWNHYVGDADRTHPYASPLRADHHRGLPP
AFVVTAEFDPLRDEGEAYARRLAEAGTPVECKRYDGTIHGFCWMPGVLDKGRQALDDTAAALAAAFQSDNR

Sequences:

>Translated_311_residues
MALDEQARYILDMIASAGRPPLHTCTPEQARQSFDFRPLAGVPDAVAQIEEVTIPGPAGDIRVRIYTPDGEGPFPALVYC
HGGGWVVGDLDTVDVPCRRLATRASCVVVSVDYRLAPEHRFPAATEDAYAAFQWLVSNARAQQVDATRIAVGGDSAGGNL
AAAVALMARDRAAPQPCFQVLLYPVTDGTLDTPSYRENAEGYLLTRDSMVWFWNHYVGDADRTHPYASPLRADHHRGLPP
AFVVTAEFDPLRDEGEAYARRLAEAGTPVECKRYDGTIHGFCWMPGVLDKGRQALDDTAAALAAAFQSDNR
>Mature_310_residues
ALDEQARYILDMIASAGRPPLHTCTPEQARQSFDFRPLAGVPDAVAQIEEVTIPGPAGDIRVRIYTPDGEGPFPALVYCH
GGGWVVGDLDTVDVPCRRLATRASCVVVSVDYRLAPEHRFPAATEDAYAAFQWLVSNARAQQVDATRIAVGGDSAGGNLA
AAVALMARDRAAPQPCFQVLLYPVTDGTLDTPSYRENAEGYLLTRDSMVWFWNHYVGDADRTHPYASPLRADHHRGLPPA
FVVTAEFDPLRDEGEAYARRLAEAGTPVECKRYDGTIHGFCWMPGVLDKGRQALDDTAAALAAAFQSDNR

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=299, Percent_Identity=32.4414715719064, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI206597554, Length=288, Percent_Identity=32.6388888888889, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI68051721, Length=189, Percent_Identity=32.8042328042328, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI226423947, Length=197, Percent_Identity=31.4720812182741, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI157041239, Length=151, Percent_Identity=36.4238410596026, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI61966717, Length=157, Percent_Identity=34.3949044585987, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI226423952, Length=145, Percent_Identity=33.1034482758621, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI226423950, Length=145, Percent_Identity=33.1034482758621, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI157041237, Length=111, Percent_Identity=36.036036036036, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786682, Length=314, Percent_Identity=32.1656050955414, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17567059, Length=287, Percent_Identity=30.3135888501742, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17540028, Length=311, Percent_Identity=26.6881028938907, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71996133, Length=308, Percent_Identity=26.2987012987013, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=146, Percent_Identity=36.986301369863, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533412, Length=108, Percent_Identity=37.962962962963, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533410, Length=120, Percent_Identity=37.5, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24656084, Length=105, Percent_Identity=38.0952380952381, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24656076, Length=105, Percent_Identity=38.0952380952381, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI20130169, Length=105, Percent_Identity=38.0952380952381, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33830; Mature: 33699

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDEQARYILDMIASAGRPPLHTCTPEQARQSFDFRPLAGVPDAVAQIEEVTIPGPAGD
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHEECCCCCCC
IRVRIYTPDGEGPFPALVYCHGGGWVVGDLDTVDVPCRRLATRASCVVVSVDYRLAPEHR
EEEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCHHHHHCCCEEEEEEECCEECCCCC
FPAATEDAYAAFQWLVSNARAQQVDATRIAVGGDSAGGNLAAAVALMARDRAAPQPCFQV
CCCCCHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHEE
LLYPVTDGTLDTPSYRENAEGYLLTRDSMVWFWNHYVGDADRTHPYASPLRADHHRGLPP
EEEEECCCCCCCCCCCCCCCEEEEEECCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCC
AFVVTAEFDPLRDEGEAYARRLAEAGTPVECKRYDGTIHGFCWMPGVLDKGRQALDDTAA
EEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHH
ALAAAFQSDNR
HHHHHHHCCCC
>Mature Secondary Structure 
ALDEQARYILDMIASAGRPPLHTCTPEQARQSFDFRPLAGVPDAVAQIEEVTIPGPAGD
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHEECCCCCCC
IRVRIYTPDGEGPFPALVYCHGGGWVVGDLDTVDVPCRRLATRASCVVVSVDYRLAPEHR
EEEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCHHHHHCCCEEEEEEECCEECCCCC
FPAATEDAYAAFQWLVSNARAQQVDATRIAVGGDSAGGNLAAAVALMARDRAAPQPCFQV
CCCCCHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHEE
LLYPVTDGTLDTPSYRENAEGYLLTRDSMVWFWNHYVGDADRTHPYASPLRADHHRGLPP
EEEEECCCCCCCCCCCCCCCEEEEEECCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCC
AFVVTAEFDPLRDEGEAYARRLAEAGTPVECKRYDGTIHGFCWMPGVLDKGRQALDDTAA
EEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHH
ALAAAFQSDNR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA