Click here to switch to the map view.

The map label for this gene is pcaQ [H]

Identifier: 73539282

GI number: 73539282

Start: 2250434

End: 2251405

Strand: Reverse

Name: pcaQ [H]

Synonym: Reut_B5460

Alternate gene names: 73539282

Gene position: 2251405-2250434 (Counterclockwise)

Preceding gene: 73539289

Following gene: 73539281

Centisome position: 82.59

GC content: 62.65

Gene sequence:

>972_bases
ATGGATGCTCGTCTGAAGCAGGCCGTCGCCGTTGGCAGGTTCGGCTCCTTCTCGAAGGCAGCGGAAGCGGTCAATGTGAC
CCAGTCGGCCGTCACCAAAAGCGTCGCGGAGCTGGAGCGGCGCCTCGGCTTCCCTATTTTCATCCGCACCTCGCGCGGCG
TAGTGCCGACCAGCGAAGGGCGCGCGTTCCTGGAGCGCGCCTCACGGCTGATTGCGGATACCGATGAACTGCTGAGCGGG
GCGCTGCGCACCGACCCGTTCAGCGGGAGCCTGCGCATCGGGATCTTTCCGACGACGTTCGAGTGGATGCTGGCAAAGCC
GCTCGAGGTGCTGGTGCAGCGTCATCCGCGGCTGCTGCTGGACATTACGTCAGGAACGAAAGAACGCGGCGTGCAACTTC
TTGACCAGGGCGACGTCGATGTTGCCATCGGACTCGAGTCGGCGTTCGGGGACAAGGCCCGCTTCAAACTCGAATACATC
GCTACGATCTCGGCATCGATATTCGTGCGGCGCGGGCATCCGCTGCTGGAGCTGGAGGCGCCGGTGGCGTCCGATATCGC
CCGGTACGACATGGTCCTTCCGGGCCAGCTCTGGGATCTGTCGCTGTACCCGATTCTCAGCCAGATCTACGGTCAGGCCG
AGGCAGTCCGGTTCCACAGGATCGAAAACTTCTCACTGCAATGCAAAGTCATCGAAAACACGGATGCGATGGGCTTTGTC
GACCAGGCCATCACGAGGACAGATTACTTCCAGTCGCGCTTTGCGGTTCTGCAGGGCCTTGAAACCGCCACCACGGCCGG
CATTGTCTGTGCCGTTCGCGAACAATGGACGCCCAAAGCCGGTGTCGGGGCACTGGTCGACATCTTGCAGCGTGTGCATG
CGGAGGGAATGCTGAACAGCGACATCCAGAACCGGCTGTTCGGACATGAATGCGCGGCGCTGGCGGCCGAAGCGGTGCCG
CACCGGGGTTGA

Upstream 100 bases:

>100_bases
CCGGCCCTGCCTCGCACGGATCCGGTTGACAGGGCTGTTCACCGCGTCCGTGGCAAAATCACCAATCATTGAAGTCCGGC
CAGCATGGGAGAGGTTGCAA

Downstream 100 bases:

>100_bases
TCCCGAGTTCTTTTTCCGACAAGCGGTGCACATTGCATTAAAGTGGCACCCTTTCCGCGATGCGACTCCCGGCCGCCAGC
CGACGTTCACGCGGCTCCAA

Product: regulatory protein LysR

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDARLKQAVAVGRFGSFSKAAEAVNVTQSAVTKSVAELERRLGFPIFIRTSRGVVPTSEGRAFLERASRLIADTDELLSG
ALRTDPFSGSLRIGIFPTTFEWMLAKPLEVLVQRHPRLLLDITSGTKERGVQLLDQGDVDVAIGLESAFGDKARFKLEYI
ATISASIFVRRGHPLLELEAPVASDIARYDMVLPGQLWDLSLYPILSQIYGQAEAVRFHRIENFSLQCKVIENTDAMGFV
DQAITRTDYFQSRFAVLQGLETATTAGIVCAVREQWTPKAGVGALVDILQRVHAEGMLNSDIQNRLFGHECAALAAEAVP
HRG

Sequences:

>Translated_323_residues
MDARLKQAVAVGRFGSFSKAAEAVNVTQSAVTKSVAELERRLGFPIFIRTSRGVVPTSEGRAFLERASRLIADTDELLSG
ALRTDPFSGSLRIGIFPTTFEWMLAKPLEVLVQRHPRLLLDITSGTKERGVQLLDQGDVDVAIGLESAFGDKARFKLEYI
ATISASIFVRRGHPLLELEAPVASDIARYDMVLPGQLWDLSLYPILSQIYGQAEAVRFHRIENFSLQCKVIENTDAMGFV
DQAITRTDYFQSRFAVLQGLETATTAGIVCAVREQWTPKAGVGALVDILQRVHAEGMLNSDIQNRLFGHECAALAAEAVP
HRG
>Mature_323_residues
MDARLKQAVAVGRFGSFSKAAEAVNVTQSAVTKSVAELERRLGFPIFIRTSRGVVPTSEGRAFLERASRLIADTDELLSG
ALRTDPFSGSLRIGIFPTTFEWMLAKPLEVLVQRHPRLLLDITSGTKERGVQLLDQGDVDVAIGLESAFGDKARFKLEYI
ATISASIFVRRGHPLLELEAPVASDIARYDMVLPGQLWDLSLYPILSQIYGQAEAVRFHRIENFSLQCKVIENTDAMGFV
DQAITRTDYFQSRFAVLQGLETATTAGIVCAVREQWTPKAGVGALVDILQRVHAEGMLNSDIQNRLFGHECAALAAEAVP
HRG

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787601, Length=170, Percent_Identity=28.8235294117647, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786508, Length=302, Percent_Identity=22.5165562913907, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI145693193, Length=171, Percent_Identity=30.4093567251462, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35472; Mature: 35472

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARLKQAVAVGRFGSFSKAAEAVNVTQSAVTKSVAELERRLGFPIFIRTSRGVVPTSEG
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
RAFLERASRLIADTDELLSGALRTDPFSGSLRIGIFPTTFEWMLAKPLEVLVQRHPRLLL
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEE
DITSGTKERGVQLLDQGDVDVAIGLESAFGDKARFKLEYIATISASIFVRRGHPLLELEA
EECCCCHHHCCEEECCCCEEEEEECHHHCCCCHHEEEEHEEHHHEEEEEECCCCEEEECC
PVASDIARYDMVLPGQLWDLSLYPILSQIYGQAEAVRFHRIENFSLQCKVIENTDAMGFV
CHHHHHHHHCEECCCCCCCCHHHHHHHHHHCCHHHHHHHEECCCEEEEEEECCCCCHHHH
DQAITRTDYFQSRFAVLQGLETATTAGIVCAVREQWTPKAGVGALVDILQRVHAEGMLNS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEHHCCCCCCCHHHHHHHHHHHHHCCCCCH
DIQNRLFGHECAALAAEAVPHRG
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDARLKQAVAVGRFGSFSKAAEAVNVTQSAVTKSVAELERRLGFPIFIRTSRGVVPTSEG
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
RAFLERASRLIADTDELLSGALRTDPFSGSLRIGIFPTTFEWMLAKPLEVLVQRHPRLLL
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEE
DITSGTKERGVQLLDQGDVDVAIGLESAFGDKARFKLEYIATISASIFVRRGHPLLELEA
EECCCCHHHCCEEECCCCEEEEEECHHHCCCCHHEEEEHEEHHHEEEEEECCCCEEEECC
PVASDIARYDMVLPGQLWDLSLYPILSQIYGQAEAVRFHRIENFSLQCKVIENTDAMGFV
CHHHHHHHHCEECCCCCCCCHHHHHHHHHHCCHHHHHHHEECCCEEEEEEECCCCCHHHH
DQAITRTDYFQSRFAVLQGLETATTAGIVCAVREQWTPKAGVGALVDILQRVHAEGMLNS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEHHCCCCCCCHHHHHHHHHHHHHCCCCCH
DIQNRLFGHECAALAAEAVPHRG
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]