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The map label for this gene is asnB [H]

Identifier: 73539195

GI number: 73539195

Start: 2153546

End: 2155576

Strand: Reverse

Name: asnB [H]

Synonym: Reut_B5373

Alternate gene names: 73539195

Gene position: 2155576-2153546 (Counterclockwise)

Preceding gene: 73539196

Following gene: 73539194

Centisome position: 79.07

GC content: 64.3

Gene sequence:

>2031_bases
ATGTGTGGATTTGCAGGATTTCTTGGCGGTATACCGGGTTCCCTGGAAGATCGTGCGATCTTGCTGACCCGTATGGGACA
GCGGATCCTTCACCGGGGTCCGGACCATGGCGACCTTTGGTGCGAGCCCGGCGCGCCTTTCGGCTTTACGCACCGGCGCC
TCGCAATTGTCGATCTGTCCCCGGCCGGTCGACAGCCGATGATTTCGGCGAGCGGCCGCTACGTCATTGTGTTCAATGGC
GAAATTTACAACCATCCCGAAATACGCGCCGAACTCGAAAGCGCAGGGGCCGCGCCGCGTTGGCGAGGGCACTCCGACAC
CGAAACGCTGCTTGCCGGCATCGACGCATGGGGAGCCGATGCCACGGTTCGGCGAACCATCGGGATGTTTGCCTTTGCCG
TGTGGGACCGGCAAACGCATTCCCTGACGCTGGCGCGTGACCGGCTGGGCGAGAAGCCGCTCTACTATGGCTGGCAGGGC
TCAGGCCAGGCGCGTGCCTTTCTATTCGGATCAGAGCTCAAGGCACTGCGCCCTCACCCGGCATTCGACGGCAGCATCGA
CCGCAACGCCCTCACCTTGTACATGCGCTACAACAATGTCGGTGGCGGGCATTCGATCTACCAAGGCATCGCCAAGCTTC
CACCTGCGCACTTGCTGACCGTGTCGATGCTGGCGCGCGAGCCTGTTTTGCGTGCCTACTGGTCGGGGTCCGCGGCAGCG
CTGCAAGGTAAGCAGGACCCTTTCACGGGCGGTCCCCAAGAAGCCGTCGACAGCCTCGATGCGCTGTTGCGAGATGCTGT
CGCCCGCCAGATGATGGCTGACGTGCCGCTGGGCGCATTTCTCTCCGGTGGTATCGATTCGTCGACCGTGGTCGCACTGA
TGCAGTCCCAGTCCACACAGCCGATCCGCACGTTCTCGATCGGCTTCCACGACGCCGGCTACAACGAGGCCGCACACGCC
AAGGCCGTCGCCCGCCACCTCGGCACCAGCCACACCGAGCTGTATGTGTCCCCCGAGCATGCCATGGCGGTGATTCCGCG
ATTGCCGGAGATCTACGACGAGCCATTCTCCGACTCTTCGCAAATCCCCACGCTGCTTGTTAGCGGACTGGCCAGGCAGC
ACGTGACGGTGTCCCTCTCCGGCGACGCGGGAGACGAGTTGTTCGGTGGATATAACCGCTACCAGATCACGGCAGAGCTT
TGGGGCCGGCTTGCCCGCATCCCCGGTCCGGTGCGCGCCGCGGCGGCCTGGGGGCTGACCCGTCTTTCTCCGCACCAGAT
CAATCGCGTCGCAGCGCTGCTGCCAATGGCATCGCGCTGGTCCCATGTGGGGGAGAAGGTCCACAAGGGCGCGGGCGTCA
TGGCCGCGCACACCGTGGATGAACTCTATCGCGGCATGGTCTCCCAGTGGCAATGTCCGGATGCAATCGTCATGGGCGGG
GCGGAGCCGGCAACCTTGCCTGAGCCCGTTGCAAGGGCGCTGGCGATGCTCGGCGACGTGGAACGCATGATGGCGGCCGA
TCTGCTGACCTACCTGCCCGACGACATCCTCACGAAGGTCGACCGCGCGGCCATGGCGCGAAGCCTTGAAACTCGCATCC
CCTTCCTCGATCACCGCGTGGTGGAGTTCGCCTGGCGGCTGCCGCTGGCCTACAAGATCCGGCAGGAGGACGCCGGGCAC
ACGACCAAATGGGTACTCAGGCGCGTGCTTGACCGCTATGTGCCACGCGAGCTGATCGAGCGGCCGAAGATGGGATTTGG
CGTCCCGATCGACGCATGGCTGCGTGGTCCGCTGCGCGACTGGGCCGACGATCTTCTGAGCGAGGAGCGCCTGCGTCGCG
AGGGCTTCCTGAATCCGCAACCCATACGCCAGCGTTGGACTGAGCATCTGTCCGGGCGGCGCAACTGGCAGCATCCCCTA
TGGTGTGTGCTCATGTTCCAGGCCTGGCTAGGGCACGAGACAACAGAGCGGACCGCACCGCCGCTGACTGATATCCCGAC
ATCCGATAGGAGGCTGGCATGCGCTTTCTGA

Upstream 100 bases:

>100_bases
CCGCATCGGTGACGGGCTGGCCGAGGCAATGCACTGGTATGTGCAGTTGCACCGGACGCAGGACGCCTGAGCAGCACCGA
AGACAAAACGGAAGAGCGCG

Downstream 100 bases:

>100_bases
TGATCGCGAGCTACGCGGAGTCGCTGATCGCGTTTCGCGGGCCGCTGATGGAAGCATTGCAGGATAGCGGACTTGAAATG
CATGTCGCGGCGCCGGGGCT

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MCGFAGFLGGIPGSLEDRAILLTRMGQRILHRGPDHGDLWCEPGAPFGFTHRRLAIVDLSPAGRQPMISASGRYVIVFNG
EIYNHPEIRAELESAGAAPRWRGHSDTETLLAGIDAWGADATVRRTIGMFAFAVWDRQTHSLTLARDRLGEKPLYYGWQG
SGQARAFLFGSELKALRPHPAFDGSIDRNALTLYMRYNNVGGGHSIYQGIAKLPPAHLLTVSMLAREPVLRAYWSGSAAA
LQGKQDPFTGGPQEAVDSLDALLRDAVARQMMADVPLGAFLSGGIDSSTVVALMQSQSTQPIRTFSIGFHDAGYNEAAHA
KAVARHLGTSHTELYVSPEHAMAVIPRLPEIYDEPFSDSSQIPTLLVSGLARQHVTVSLSGDAGDELFGGYNRYQITAEL
WGRLARIPGPVRAAAAWGLTRLSPHQINRVAALLPMASRWSHVGEKVHKGAGVMAAHTVDELYRGMVSQWQCPDAIVMGG
AEPATLPEPVARALAMLGDVERMMAADLLTYLPDDILTKVDRAAMARSLETRIPFLDHRVVEFAWRLPLAYKIRQEDAGH
TTKWVLRRVLDRYVPRELIERPKMGFGVPIDAWLRGPLRDWADDLLSEERLRREGFLNPQPIRQRWTEHLSGRRNWQHPL
WCVLMFQAWLGHETTERTAPPLTDIPTSDRRLACAF

Sequences:

>Translated_676_residues
MCGFAGFLGGIPGSLEDRAILLTRMGQRILHRGPDHGDLWCEPGAPFGFTHRRLAIVDLSPAGRQPMISASGRYVIVFNG
EIYNHPEIRAELESAGAAPRWRGHSDTETLLAGIDAWGADATVRRTIGMFAFAVWDRQTHSLTLARDRLGEKPLYYGWQG
SGQARAFLFGSELKALRPHPAFDGSIDRNALTLYMRYNNVGGGHSIYQGIAKLPPAHLLTVSMLAREPVLRAYWSGSAAA
LQGKQDPFTGGPQEAVDSLDALLRDAVARQMMADVPLGAFLSGGIDSSTVVALMQSQSTQPIRTFSIGFHDAGYNEAAHA
KAVARHLGTSHTELYVSPEHAMAVIPRLPEIYDEPFSDSSQIPTLLVSGLARQHVTVSLSGDAGDELFGGYNRYQITAEL
WGRLARIPGPVRAAAAWGLTRLSPHQINRVAALLPMASRWSHVGEKVHKGAGVMAAHTVDELYRGMVSQWQCPDAIVMGG
AEPATLPEPVARALAMLGDVERMMAADLLTYLPDDILTKVDRAAMARSLETRIPFLDHRVVEFAWRLPLAYKIRQEDAGH
TTKWVLRRVLDRYVPRELIERPKMGFGVPIDAWLRGPLRDWADDLLSEERLRREGFLNPQPIRQRWTEHLSGRRNWQHPL
WCVLMFQAWLGHETTERTAPPLTDIPTSDRRLACAF
>Mature_676_residues
MCGFAGFLGGIPGSLEDRAILLTRMGQRILHRGPDHGDLWCEPGAPFGFTHRRLAIVDLSPAGRQPMISASGRYVIVFNG
EIYNHPEIRAELESAGAAPRWRGHSDTETLLAGIDAWGADATVRRTIGMFAFAVWDRQTHSLTLARDRLGEKPLYYGWQG
SGQARAFLFGSELKALRPHPAFDGSIDRNALTLYMRYNNVGGGHSIYQGIAKLPPAHLLTVSMLAREPVLRAYWSGSAAA
LQGKQDPFTGGPQEAVDSLDALLRDAVARQMMADVPLGAFLSGGIDSSTVVALMQSQSTQPIRTFSIGFHDAGYNEAAHA
KAVARHLGTSHTELYVSPEHAMAVIPRLPEIYDEPFSDSSQIPTLLVSGLARQHVTVSLSGDAGDELFGGYNRYQITAEL
WGRLARIPGPVRAAAAWGLTRLSPHQINRVAALLPMASRWSHVGEKVHKGAGVMAAHTVDELYRGMVSQWQCPDAIVMGG
AEPATLPEPVARALAMLGDVERMMAADLLTYLPDDILTKVDRAAMARSLETRIPFLDHRVVEFAWRLPLAYKIRQEDAGH
TTKWVLRRVLDRYVPRELIERPKMGFGVPIDAWLRGPLRDWADDLLSEERLRREGFLNPQPIRQRWTEHLSGRRNWQHPL
WCVLMFQAWLGHETTERTAPPLTDIPTSDRRLACAF

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=411, Percent_Identity=28.2238442822384, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI168229252, Length=411, Percent_Identity=28.2238442822384, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI168229250, Length=411, Percent_Identity=28.2238442822384, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI296010848, Length=384, Percent_Identity=28.90625, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI296010852, Length=308, Percent_Identity=27.5974025974026, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI296010850, Length=308, Percent_Identity=27.5974025974026, Blast_Score=87, Evalue=6e-17,
Organism=Escherichia coli, GI1786889, Length=409, Percent_Identity=28.1173594132029, Blast_Score=137, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25147557, Length=380, Percent_Identity=27.3684210526316, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71993933, Length=380, Percent_Identity=27.3684210526316, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560178, Length=414, Percent_Identity=26.0869565217391, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321563, Length=428, Percent_Identity=27.3364485981308, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6325403, Length=429, Percent_Identity=27.2727272727273, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45553209, Length=373, Percent_Identity=28.9544235924933, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 74852; Mature: 74852

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFAGFLGGIPGSLEDRAILLTRMGQRILHRGPDHGDLWCEPGAPFGFTHRRLAIVDLS
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEECC
PAGRQPMISASGRYVIVFNGEIYNHPEIRAELESAGAAPRWRGHSDTETLLAGIDAWGAD
CCCCCCEEECCCCEEEEECCCEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
ATVRRTIGMFAFAVWDRQTHSLTLARDRLGEKPLYYGWQGSGQARAFLFGSELKALRPHP
HHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCEEECCCCCCCEEEEEECCHHHHCCCCC
AFDGSIDRNALTLYMRYNNVGGGHSIYQGIAKLPPAHLLTVSMLAREPVLRAYWSGSAAA
CCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHCCCHHE
LQGKQDPFTGGPQEAVDSLDALLRDAVARQMMADVPLGAFLSGGIDSSTVVALMQSQSTQ
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCC
PIRTFSIGFHDAGYNEAAHAKAVARHLGTSHTELYVSPEHAMAVIPRLPEIYDEPFSDSS
CEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHCCCCCCCC
QIPTLLVSGLARQHVTVSLSGDAGDELFGGYNRYQITAELWGRLARIPGPVRAAAAWGLT
CHHHHHHHHHHHCEEEEEECCCCCHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHCCC
RLSPHQINRVAALLPMASRWSHVGEKVHKGAGVMAAHTVDELYRGMVSQWQCPDAIVMGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCEEECC
AEPATLPEPVARALAMLGDVERMMAADLLTYLPDDILTKVDRAAMARSLETRIPFLDHRV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
VEFAWRLPLAYKIRQEDAGHTTKWVLRRVLDRYVPRELIERPKMGFGVPIDAWLRGPLRD
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHCCHHHH
WADDLLSEERLRREGFLNPQPIRQRWTEHLSGRRNWQHPLWCVLMFQAWLGHETTERTAP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
PLTDIPTSDRRLACAF
CCCCCCCCCCEEEECC
>Mature Secondary Structure
MCGFAGFLGGIPGSLEDRAILLTRMGQRILHRGPDHGDLWCEPGAPFGFTHRRLAIVDLS
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEECC
PAGRQPMISASGRYVIVFNGEIYNHPEIRAELESAGAAPRWRGHSDTETLLAGIDAWGAD
CCCCCCEEECCCCEEEEECCCEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
ATVRRTIGMFAFAVWDRQTHSLTLARDRLGEKPLYYGWQGSGQARAFLFGSELKALRPHP
HHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCEEECCCCCCCEEEEEECCHHHHCCCCC
AFDGSIDRNALTLYMRYNNVGGGHSIYQGIAKLPPAHLLTVSMLAREPVLRAYWSGSAAA
CCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHCCCHHE
LQGKQDPFTGGPQEAVDSLDALLRDAVARQMMADVPLGAFLSGGIDSSTVVALMQSQSTQ
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCC
PIRTFSIGFHDAGYNEAAHAKAVARHLGTSHTELYVSPEHAMAVIPRLPEIYDEPFSDSS
CEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHCCCCCCCC
QIPTLLVSGLARQHVTVSLSGDAGDELFGGYNRYQITAELWGRLARIPGPVRAAAAWGLT
CHHHHHHHHHHHCEEEEEECCCCCHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHCCC
RLSPHQINRVAALLPMASRWSHVGEKVHKGAGVMAAHTVDELYRGMVSQWQCPDAIVMGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCEEECC
AEPATLPEPVARALAMLGDVERMMAADLLTYLPDDILTKVDRAAMARSLETRIPFLDHRV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
VEFAWRLPLAYKIRQEDAGHTTKWVLRRVLDRYVPRELIERPKMGFGVPIDAWLRGPLRD
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHCCHHHH
WADDLLSEERLRREGFLNPQPIRQRWTEHLSGRRNWQHPLWCVLMFQAWLGHETTERTAP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
PLTDIPTSDRRLACAF
CCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]