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The map label for this gene is vipB [H]

Identifier: 73539196

GI number: 73539196

Start: 2155607

End: 2156740

Strand: Reverse

Name: vipB [H]

Synonym: Reut_B5374

Alternate gene names: 73539196

Gene position: 2156740-2155607 (Counterclockwise)

Preceding gene: 73539197

Following gene: 73539195

Centisome position: 79.11

GC content: 62.87

Gene sequence:

>1134_bases
ATGTCGACGCCCGCCTCTAGAAGCGCACCGCCACGAAGAGCTCACATGGAATCCATCACCTACGCGAATCTGAGGACCAG
TCTGGTTGCCAATCCGTGCACGTGGCTGGTCACAGGAGTGGCCGGATTCATCGGCAGCAATTTGCTGGAGGCCTTGCTGC
GCCTGAACCAGCGCGTGGTCGGCCTGGACAACTTCTTGACCGGCCACCGCCGCAACCTGCATGAAGTGCAGGCGCTGGTA
TCTCCCGCGCAGTGGGAGAACTTTCAATTCATCGAAGGCGACATCCGGGACCTTGAACAGTGCCGGAGCGCCATGCGCTT
TTGCTCCGGGGAGCGCGAAGGAACGCACGGCGCGAGCAGCCATCCGGTCGATTTCGTGCTGCACCAGGCCGCGCTGGGCT
CCGTGCCACGCAGCCTGGAGGACCCCATCGCGACCCACAGCGTCAACATCAGTGGCTTCCTGAATATGCTGGTCGCTGCG
CGCGACGCCGAGGTACGCGCTTTCGTTTACGCGGCGAGCAGCAGCACCTACGGCGATCACCCCGGCCTGCCCAAGACCGA
AGACAGGATCGGCAAGCCATTGAGCCCCTACGCGGTCACGAAATACGCGAACGAGTTGTATGCCGACGTCTTCAGGCGCT
GCTACAGTTTGCGTGCCATCGGACTACGCTACTTCAATGTATTCGGTCCACGCCAGGATCCGAACGGGGCATACGCAGCC
GTGATTCCGCGGTGGATTTCCGCGTTGCTGCGGCGCGAGACCGTCTACATCAATGGCGACGGCGAAACGAGCCGCGACTT
CTGTTACGTGGCGAATGCTGTGCAGGCCAACCTGCTTGCCGCTCTCAGCTGCAGCGGAGACGCGGAAAGCCAGATCTACA
ACGTCGCCGTCGGTGACCGCACCACGCTGAGTACCCTGTTCCAGCTTCTGCGCGACGGGCTTCGTCCTTATGGCATCCCC
GCGGACACGCAGCCGACCTATCGGGCCTTCCGTGCGGGCGACGTGCGCCACAGCCAGGCTGATATCGGCAAGGCACAACG
GTTGCTCGGATATCAGCCCACCCACCGCATCGGTGACGGGCTGGCCGAGGCAATGCACTGGTATGTGCAGTTGCACCGGA
CGCAGGACGCCTGA

Upstream 100 bases:

>100_bases
CCCCATCATCAGTTCGTCACGATGGGGGTGCGGGCGATCAGGGCCTTTGGCAAATCGCACGCCGTGCTGTTCGACGTAAA
GGGCATCTTGCCGAGGAGCG

Downstream 100 bases:

>100_bases
GCAGCACCGAAGACAAAACGGAAGAGCGCGATGTGTGGATTTGCAGGATTTCTTGGCGGTATACCGGGTTCCCTGGAAGA
TCGTGCGATCTTGCTGACCC

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSTPASRSAPPRRAHMESITYANLRTSLVANPCTWLVTGVAGFIGSNLLEALLRLNQRVVGLDNFLTGHRRNLHEVQALV
SPAQWENFQFIEGDIRDLEQCRSAMRFCSGEREGTHGASSHPVDFVLHQAALGSVPRSLEDPIATHSVNISGFLNMLVAA
RDAEVRAFVYAASSSTYGDHPGLPKTEDRIGKPLSPYAVTKYANELYADVFRRCYSLRAIGLRYFNVFGPRQDPNGAYAA
VIPRWISALLRRETVYINGDGETSRDFCYVANAVQANLLAALSCSGDAESQIYNVAVGDRTTLSTLFQLLRDGLRPYGIP
ADTQPTYRAFRAGDVRHSQADIGKAQRLLGYQPTHRIGDGLAEAMHWYVQLHRTQDA

Sequences:

>Translated_377_residues
MSTPASRSAPPRRAHMESITYANLRTSLVANPCTWLVTGVAGFIGSNLLEALLRLNQRVVGLDNFLTGHRRNLHEVQALV
SPAQWENFQFIEGDIRDLEQCRSAMRFCSGEREGTHGASSHPVDFVLHQAALGSVPRSLEDPIATHSVNISGFLNMLVAA
RDAEVRAFVYAASSSTYGDHPGLPKTEDRIGKPLSPYAVTKYANELYADVFRRCYSLRAIGLRYFNVFGPRQDPNGAYAA
VIPRWISALLRRETVYINGDGETSRDFCYVANAVQANLLAALSCSGDAESQIYNVAVGDRTTLSTLFQLLRDGLRPYGIP
ADTQPTYRAFRAGDVRHSQADIGKAQRLLGYQPTHRIGDGLAEAMHWYVQLHRTQDA
>Mature_376_residues
STPASRSAPPRRAHMESITYANLRTSLVANPCTWLVTGVAGFIGSNLLEALLRLNQRVVGLDNFLTGHRRNLHEVQALVS
PAQWENFQFIEGDIRDLEQCRSAMRFCSGEREGTHGASSHPVDFVLHQAALGSVPRSLEDPIATHSVNISGFLNMLVAAR
DAEVRAFVYAASSSTYGDHPGLPKTEDRIGKPLSPYAVTKYANELYADVFRRCYSLRAIGLRYFNVFGPRQDPNGAYAAV
IPRWISALLRRETVYINGDGETSRDFCYVANAVQANLLAALSCSGDAESQIYNVAVGDRTTLSTLFQLLRDGLRPYGIPA
DTQPTYRAFRAGDVRHSQADIGKAQRLLGYQPTHRIGDGLAEAMHWYVQLHRTQDA

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=341, Percent_Identity=28.1524926686217, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI7657641, Length=347, Percent_Identity=27.0893371757925, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI56237023, Length=344, Percent_Identity=27.0348837209302, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI56118217, Length=344, Percent_Identity=27.0348837209302, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI189083684, Length=344, Percent_Identity=27.0348837209302, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=27.8735632183908, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=28.2122905027933, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994969, Length=356, Percent_Identity=27.5280898876405, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790049, Length=344, Percent_Identity=25.5813953488372, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=351, Percent_Identity=26.4957264957265, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=365, Percent_Identity=27.1232876712329, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=367, Percent_Identity=26.9754768392371, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=343, Percent_Identity=25.3644314868805, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=362, Percent_Identity=24.3093922651934, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=26.536312849162, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=28.133704735376, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356223, Length=341, Percent_Identity=26.099706744868, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 41656; Mature: 41525

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPASRSAPPRRAHMESITYANLRTSLVANPCTWLVTGVAGFIGSNLLEALLRLNQRVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
GLDNFLTGHRRNLHEVQALVSPAQWENFQFIEGDIRDLEQCRSAMRFCSGEREGTHGASS
CHHHHHHHCCCCHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
HPVDFVLHQAALGSVPRSLEDPIATHSVNISGFLNMLVAARDAEVRAFVYAASSSTYGDH
CCHHHHHHHHHHHCCCCHHHCCHHHCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
PGLPKTEDRIGKPLSPYAVTKYANELYADVFRRCYSLRAIGLRYFNVFGPRQDPNGAYAA
CCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VIPRWISALLRRETVYINGDGETSRDFCYVANAVQANLLAALSCSGDAESQIYNVAVGDR
HHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEECCCCCCHHEEEEECCCC
TTLSTLFQLLRDGLRPYGIPADTQPTYRAFRAGDVRHSQADIGKAQRLLGYQPTHRIGDG
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LAEAMHWYVQLHRTQDA
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STPASRSAPPRRAHMESITYANLRTSLVANPCTWLVTGVAGFIGSNLLEALLRLNQRVV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
GLDNFLTGHRRNLHEVQALVSPAQWENFQFIEGDIRDLEQCRSAMRFCSGEREGTHGASS
CHHHHHHHCCCCHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
HPVDFVLHQAALGSVPRSLEDPIATHSVNISGFLNMLVAARDAEVRAFVYAASSSTYGDH
CCHHHHHHHHHHHCCCCHHHCCHHHCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
PGLPKTEDRIGKPLSPYAVTKYANELYADVFRRCYSLRAIGLRYFNVFGPRQDPNGAYAA
CCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VIPRWISALLRRETVYINGDGETSRDFCYVANAVQANLLAALSCSGDAESQIYNVAVGDR
HHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEECCCCCCHHEEEEECCCC
TTLSTLFQLLRDGLRPYGIPADTQPTYRAFRAGDVRHSQADIGKAQRLLGYQPTHRIGDG
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LAEAMHWYVQLHRTQDA
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]