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The map label for this gene is ligT [C]

Identifier: 73539136

GI number: 73539136

Start: 2077002

End: 2077583

Strand: Reverse

Name: ligT [C]

Synonym: Reut_B5313

Alternate gene names: 73539136

Gene position: 2077583-2077002 (Counterclockwise)

Preceding gene: 73539137

Following gene: 73539135

Centisome position: 76.21

GC content: 70.96

Gene sequence:

>582_bases
GTGGCACGCCTGTTCATCGCCGTCGACGCGCCGCCTGAGATAGCGGCGCGGCTGGTGAAGAGCGTGCCGACAGCCAGCGG
CATCCGCGCTGCACCGACCGGGCAGGTGCACCTGACCTTGCGCTTTCTCGGGGAGTGCGACGCGGAGCAGACCGAGCGCA
TCCGCGCCGCGCTCGACGCCGTCCGTGCGGCGGACTTCACGCTGAACGTGGCTGGCGTCGGCCGCTTTCGCGGCCGGCAG
GGCAGCGTGCTGTGGGCAGGCATGGCGCCGTGCCCTGAGCTCGATGCGCTGTATGTGGCCATCACCGCCGCGCTGCAGTC
CGTCGGTATCGCGCCGGAGCCGCGGCGGTTTCGCCCGCACCTGACTCTTGCGCGCTGTCGCCCCACGGCGCCGGAAGTGC
TGCAACGCGAATGGCTGGCCAATCATCGCGACATGGCCGTGCCGCCGTGGCACGCGCGGCGCTTCGTGCTGTACGAAAGC
CGCCTGGAGCGGCAAGGCGCGACGCATGCCGAGATCGGGTCGTGGCCGCTCATGGCACCGGATACTCCTCCTGCGACGGC
GAGCGTACCCGTTTCTGCGTGA

Upstream 100 bases:

>100_bases
ACCGATCGCAAGCGCGCGCTGCTCCGGCTCGAGGGCCACGCTGCCGCAGCGCGTCTGCCGATCATGCAGCAGTCCGCTCT
CGCCTTCGTGTAGGTCCGGC

Downstream 100 bases:

>100_bases
CGCAGCGTGACAAAAACCGCGCGCCGCTGCGCGAAAGCGGCGTGAACGCTACCCCCCGAAAGTCTGTGCTCAAGTTTTGC
CACAGATTGCCGAAGCCCAT

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MARLFIAVDAPPEIAARLVKSVPTASGIRAAPTGQVHLTLRFLGECDAEQTERIRAALDAVRAADFTLNVAGVGRFRGRQ
GSVLWAGMAPCPELDALYVAITAALQSVGIAPEPRRFRPHLTLARCRPTAPEVLQREWLANHRDMAVPPWHARRFVLYES
RLERQGATHAEIGSWPLMAPDTPPATASVPVSA

Sequences:

>Translated_193_residues
MARLFIAVDAPPEIAARLVKSVPTASGIRAAPTGQVHLTLRFLGECDAEQTERIRAALDAVRAADFTLNVAGVGRFRGRQ
GSVLWAGMAPCPELDALYVAITAALQSVGIAPEPRRFRPHLTLARCRPTAPEVLQREWLANHRDMAVPPWHARRFVLYES
RLERQGATHAEIGSWPLMAPDTPPATASVPVSA
>Mature_192_residues
ARLFIAVDAPPEIAARLVKSVPTASGIRAAPTGQVHLTLRFLGECDAEQTERIRAALDAVRAADFTLNVAGVGRFRGRQG
SVLWAGMAPCPELDALYVAITAALQSVGIAPEPRRFRPHLTLARCRPTAPEVLQREWLANHRDMAVPPWHARRFVLYESR
LERQGATHAEIGSWPLMAPDTPPATASVPVSA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20969; Mature: 20838

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLFIAVDAPPEIAARLVKSVPTASGIRAAPTGQVHLTLRFLGECDAEQTERIRAALDA
CCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
VRAADFTLNVAGVGRFRGRQGSVLWAGMAPCPELDALYVAITAALQSVGIAPEPRRFRPH
HHHCCEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
LTLARCRPTAPEVLQREWLANHRDMAVPPWHARRFVLYESRLERQGATHAEIGSWPLMAP
EEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHEEEEEHHHHHHCCCCCCCCCCCCCCCC
DTPPATASVPVSA
CCCCCCCCCCCCC
>Mature Secondary Structure 
ARLFIAVDAPPEIAARLVKSVPTASGIRAAPTGQVHLTLRFLGECDAEQTERIRAALDA
CEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
VRAADFTLNVAGVGRFRGRQGSVLWAGMAPCPELDALYVAITAALQSVGIAPEPRRFRPH
HHHCCEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
LTLARCRPTAPEVLQREWLANHRDMAVPPWHARRFVLYESRLERQGATHAEIGSWPLMAP
EEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHEEEEEHHHHHHCCCCCCCCCCCCCCCC
DTPPATASVPVSA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]