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The map label for this gene is ogt [H]

Identifier: 73539137

GI number: 73539137

Start: 2077591

End: 2078124

Strand: Reverse

Name: ogt [H]

Synonym: Reut_B5314

Alternate gene names: 73539137

Gene position: 2078124-2077591 (Counterclockwise)

Preceding gene: 73539138

Following gene: 73539136

Centisome position: 76.23

GC content: 67.42

Gene sequence:

>534_bases
ATGATCAGCTACCGCCAGATCGACAGCCCGCTCGGCGCCATGCTGTTGCGTGTGCAGGACAGCTTCCTGACGGGCGTTTA
TTTCGAGGGGCAGAAGTACTATCCGGCCGCGGCAGTGTCCAGCGTGGTGCCGGAAAGCGCCGCACGCATCTTCGACGAGA
CCGCCAGACAGATTGCCGCGTATTTCGATGGATTACTCGAAGCCTTCTCGGTGCCCATGCGCCTCGTTGGCACGGACTTC
CAGAGGCGCGTGTGGGAGGCGCTGCGTGCGATCCCGTTCGGCGAGACGTTGTCCTATGGCGATCTCGCCGGACAGCTTGG
GCTGCCGCCGGGGCACGCGCGCGCGGTCGGCGGCGCGGTGGGACGCAATCCCGTTTCGGTGATCGTGCCGTGCCATCGCG
TGCTTGGCGCGGCCGGCGACCTGACCGGCTATGCCGGCGGCACCGATCGCAAGCGCGCGCTGCTCCGGCTCGAGGGCCAC
GCTGCCGCAGCGCGTCTGCCGATCATGCAGCAGTCCGCTCTCGCCTTCGTGTAG

Upstream 100 bases:

>100_bases
ACTTGTGGCATCGCTATGAAGCGATGAAAACGCAAGGTGACGGCGCAGTGATACCTTTGCCCGACATAATTGCCAGCCAT
TCCGATATCGAGGTGACGAC

Downstream 100 bases:

>100_bases
GTCCGGCGTGGCACGCCTGTTCATCGCCGTCGACGCGCCGCCTGAGATAGCGGCGCGGCTGGTGAAGAGCGTGCCGACAG
CCAGCGGCATCCGCGCTGCA

Product: methylated-DNA-(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MISYRQIDSPLGAMLLRVQDSFLTGVYFEGQKYYPAAAVSSVVPESAARIFDETARQIAAYFDGLLEAFSVPMRLVGTDF
QRRVWEALRAIPFGETLSYGDLAGQLGLPPGHARAVGGAVGRNPVSVIVPCHRVLGAAGDLTGYAGGTDRKRALLRLEGH
AAAARLPIMQQSALAFV

Sequences:

>Translated_177_residues
MISYRQIDSPLGAMLLRVQDSFLTGVYFEGQKYYPAAAVSSVVPESAARIFDETARQIAAYFDGLLEAFSVPMRLVGTDF
QRRVWEALRAIPFGETLSYGDLAGQLGLPPGHARAVGGAVGRNPVSVIVPCHRVLGAAGDLTGYAGGTDRKRALLRLEGH
AAAARLPIMQQSALAFV
>Mature_177_residues
MISYRQIDSPLGAMLLRVQDSFLTGVYFEGQKYYPAAAVSSVVPESAARIFDETARQIAAYFDGLLEAFSVPMRLVGTDF
QRRVWEALRAIPFGETLSYGDLAGQLGLPPGHARAVGGAVGRNPVSVIVPCHRVLGAAGDLTGYAGGTDRKRALLRLEGH
AAAARLPIMQQSALAFV

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=36.7469879518072, Blast_Score=81, Evalue=5e-16,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=54.2857142857143, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1788542, Length=150, Percent_Identity=38, Blast_Score=97, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=92, Percent_Identity=46.7391304347826, Blast_Score=83, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=92, Percent_Identity=46.7391304347826, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=42.2222222222222, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18936; Mature: 18936

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISYRQIDSPLGAMLLRVQDSFLTGVYFEGQKYYPAAAVSSVVPESAARIFDETARQIAA
CCCHHHHCCHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHH
YFDGLLEAFSVPMRLVGTDFQRRVWEALRAIPFGETLSYGDLAGQLGLPPGHARAVGGAV
HHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCHHHHHCCCC
GRNPVSVIVPCHRVLGAAGDLTGYAGGTDRKRALLRLEGHAAAARLPIMQQSALAFV
CCCCCEEEECHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MISYRQIDSPLGAMLLRVQDSFLTGVYFEGQKYYPAAAVSSVVPESAARIFDETARQIAA
CCCHHHHCCHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHH
YFDGLLEAFSVPMRLVGTDFQRRVWEALRAIPFGETLSYGDLAGQLGLPPGHARAVGGAV
HHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCHHHHHCCCC
GRNPVSVIVPCHRVLGAAGDLTGYAGGTDRKRALLRLEGHAAAARLPIMQQSALAFV
CCCCCEEEECHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]