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The map label for this gene is argG [H]

Identifier: 73539065

GI number: 73539065

Start: 1991953

End: 1993278

Strand: Reverse

Name: argG [H]

Synonym: Reut_B5242

Alternate gene names: 73539065

Gene position: 1993278-1991953 (Counterclockwise)

Preceding gene: 73539069

Following gene: 73539064

Centisome position: 73.12

GC content: 62.82

Gene sequence:

>1326_bases
ATGACTACCATCCTTCAGCACATTCCCACCGGCGAGCGCGTCGGCATTGCGTTCTCTGGCGGCCTGGACACCAGCGCGGC
GCTTCTCTGGATGCGCCAGAAGGGCGCCATCCCTTACGCCTACACGGCCAATCTGGGCCAGCCCGACGAGCCTGACTACG
ACGACATCCCGCGCCGCGCCAAGGCCTACGGCGCCGAGGAAGCCCGCCTGGTGGACTGCCGCACGCAGCTCGTGGCCGAG
GGCATCGCTGCATTGCAGTGCGGTGCGTTCCACATTTCCACCGCCGGCGTCACGTACTTCAACACCACCCCGATCGGCCG
CGCCGTCACTGGCACCATGTTGGTTGCCGCGATGAAGGAAGATGGCGTCAATATCTGGGGTGATGGCAGCACGTTCAAAG
GCAATGATATCGAGCGTTTCTACCGCTACGGCCTCCTGACCAACCCGGGCCTGCAGATCTACAAGCCGTGGCTGGACCAG
CAGTTCATCGACGAACTTGGCGGCCGCGCCGAGATGTCCGAGTTCATGCGCCAGAACGGCTTCGACTACAAGATGTCGGC
TGAAAAGGCGTACTCGACGGATTCGAACATGCTGGGCGCCACCCACGAGGCCAAGGATCTGGAGCACCTGGATTCGGGCA
TCCGCATCGTGCAGCCGATCATGGGCGTGCAGTTCTGGCGTGACGAAGTCGAAGTCAAGCGCGAGGAAGTCACCGTGCGC
TTCGAGCAGGGCCAGCCCGTTGCGCTGAACGGCAAGACCTTCGCCAACAGCGTCGAGCTGTTCGCCGAAGCCAACGTCAT
CGGCGGCCGCCATGGCCTGGGCATGAGCGACCAGATCGAGAACCGTATCATCGAAGCCAAGAGCCGCGGCATCTACGAAG
CCCCGGGCCTGGCACTGCTGTTCATCGCGTACGAGCGCCTGATTACCGGCATCCATAACGAAGACACCATCGAGCAGTAC
CGCGACAACGGCCGCCGCCTTGGCCGCCTTCTGTACCAGGGCCGCTGGTTCGACCCGCAAGCCATCATGCTGCGCGAAAC
CGCCCAGCGCTGGGTGGCGGGTGCCGTGACCGGCGAAGTGACCATTGAACTGCGCCGCGGCAATGATTACTCGATCCTGA
ACACCACCTCGCCGAACCTGACCTACAAGCCGGAACGGCTGACCATGGAGAAGGGCGAGTCGATGTTCACGCCGCTGGAC
CGTATTGGCCAGCTGACCATGCGTACGCTGGATATCGTGGATACTCGCGAGAAGCTGCAGACGTATGCGAAGACGGGGCT
GCTGTCGTCGGCGGTTAGTGCTTCGTTGCCGAAGCTGGAAGACTGA

Upstream 100 bases:

>100_bases
ACCTTGATTCGGCCCGTGACCCGGCCAGCCGAGTCCCAGCGCTTCGACCGCGGCCGAAAGCTGCTTCAAGAGCCGACAAA
TTCACATAGATTTTCTGGCG

Downstream 100 bases:

>100_bases
GCGTACTCGCGGTGACAACGCTGCGTGAATGCGCGGCGCATCATAAAAAACCGGCACTTCGTGGTGCCGGTTTTTTCTTT
GTGATCTGTCCCAAACGACG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTTILQHIPTGERVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRAKAYGAEEARLVDCRTQLVAE
GIAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKEDGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQ
QFIDELGGRAEMSEFMRQNGFDYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDEVEVKREEVTVR
FEQGQPVALNGKTFANSVELFAEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLFIAYERLITGIHNEDTIEQY
RDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEVTIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLD
RIGQLTMRTLDIVDTREKLQTYAKTGLLSSAVSASLPKLED

Sequences:

>Translated_441_residues
MTTILQHIPTGERVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRAKAYGAEEARLVDCRTQLVAE
GIAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKEDGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQ
QFIDELGGRAEMSEFMRQNGFDYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDEVEVKREEVTVR
FEQGQPVALNGKTFANSVELFAEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLFIAYERLITGIHNEDTIEQY
RDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEVTIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLD
RIGQLTMRTLDIVDTREKLQTYAKTGLLSSAVSASLPKLED
>Mature_440_residues
TTILQHIPTGERVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRAKAYGAEEARLVDCRTQLVAEG
IAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKEDGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQQ
FIDELGGRAEMSEFMRQNGFDYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDEVEVKREEVTVRF
EQGQPVALNGKTFANSVELFAEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLFIAYERLITGIHNEDTIEQYR
DNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEVTIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLDR
IGQLTMRTLDIVDTREKLQTYAKTGLLSSAVSASLPKLED

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=406, Percent_Identity=28.0788177339901, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI16950633, Length=406, Percent_Identity=28.0788177339901, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1789563, Length=441, Percent_Identity=80.4988662131519, Blast_Score=753, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=28.537170263789, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21358151, Length=421, Percent_Identity=27.3159144893112, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 49219; Mature: 49088

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTILQHIPTGERVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRA
CCCHHHHCCCCCEEEEEEECCCCCHHHHEEEHHCCCCCEEEECCCCCCCCCCHHHCCHHH
KAYGAEEARLVDCRTQLVAEGIAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKE
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCHHHHHHHHHEEHHC
DGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQQFIDELGGRAEMSEFMRQNG
CCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCEECHHCCHHHHHHHCCHHHHHHHHHHCC
FDYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDEVEVKREEVTVR
CCEEECCCHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEEEEE
FEQGQPVALNGKTFANSVELFAEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALL
EECCCEEEECCCHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FIAYERLITGIHNEDTIEQYRDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEE
TIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLDRIGQLTMRTLDIVDTREKLQ
EEEEECCCCEEEEECCCCCCEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TYAKTGLLSSAVSASLPKLED
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTILQHIPTGERVGIAFSGGLDTSAALLWMRQKGAIPYAYTANLGQPDEPDYDDIPRRA
CCHHHHCCCCCEEEEEEECCCCCHHHHEEEHHCCCCCEEEECCCCCCCCCCHHHCCHHH
KAYGAEEARLVDCRTQLVAEGIAALQCGAFHISTAGVTYFNTTPIGRAVTGTMLVAAMKE
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCHHHHHHHHHEEHHC
DGVNIWGDGSTFKGNDIERFYRYGLLTNPGLQIYKPWLDQQFIDELGGRAEMSEFMRQNG
CCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCEECHHCCHHHHHHHCCHHHHHHHHHHCC
FDYKMSAEKAYSTDSNMLGATHEAKDLEHLDSGIRIVQPIMGVQFWRDEVEVKREEVTVR
CCEEECCCHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEEEEE
FEQGQPVALNGKTFANSVELFAEANVIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALL
EECCCEEEECCCHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FIAYERLITGIHNEDTIEQYRDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVAGAVTGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEE
TIELRRGNDYSILNTTSPNLTYKPERLTMEKGESMFTPLDRIGQLTMRTLDIVDTREKLQ
EEEEECCCCEEEEECCCCCCEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TYAKTGLLSSAVSASLPKLED
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]