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The map label for this gene is cysJ [H]

Identifier: 73539034

GI number: 73539034

Start: 1957978

End: 1959513

Strand: Reverse

Name: cysJ [H]

Synonym: Reut_B5211

Alternate gene names: 73539034

Gene position: 1959513-1957978 (Counterclockwise)

Preceding gene: 73539035

Following gene: 73539032

Centisome position: 71.88

GC content: 68.1

Gene sequence:

>1536_bases
ATGCTGAGCCCGTCGCGCGCCGGCATGGCAGCCGGCGTGTTGCTGGCCTATGCGGGCTTCTGCGGCGCGGTGTTCGACCA
CCACCGCCGCCGGCGCGCGCGCGTTGCGTCGCTTGCCGTCAGCGGCGCGCATCAGGAAGGGGCGACGCTGGTGGCTTACG
CAAGCCAGACCGGCTTCGCCGAGCAACTGGCCATGCAGACCGCCACGGCGCTGCAGGGCGCTGGCATGCCGGTGCATCTG
CTCTCGTTCGAAGAGCTCGATGCGCGCCGCCTCGCCCGCGTCCGGCAGGCGCTGTTCGTGGTCAGCACGACCGGCGAAGG
CGATGCACCGGACAGTGCCTCCGGCTTTGCGCGCCGCCTGCTGGCTGGTGGCGGCCACGATGCACTGCGCCAGCTTCGCT
ATGGCGTGCTGGCGCTCGGCGATAGCAGCTATGCGCGCTTTTGCGCGTTTGGCCATGCGTTGTCGGATTGGCTGCAGCGC
CACCATGCAAGGCCTTTATTCGATCTGATCGAGGTCGACAACGGTGATGCAGGCGCCCTGCGGCACTGGCAGAACCACTT
GTCCGCGCTCAGCGGCGGCGCGGAGATCGCCGACTGGGAGCGTCCGCGTTATGAGCATTGGCAGCTCGCTGCGCGCGAAC
ACTTGAACCCCGGAAGCGATGGCGCACCGGCGTTTCACCTCGTGCTCAGGCCCGAGGGCGCCGTGCCGGGCTGGCAGGCC
GGCGATATTGCTGAAGTTGGTCCTTGCCATGATCCGGCCGATATCGGCCGCTTGCTGAGCCACCTGAGCATGGACGGCGC
CGCGCCGGTGCGCTGCGATGCGCAGACGGTGACGTTGGCCGAAGCGCTGGCCACGCGGCTGTTGCTGCCCGAAGCGCATC
TGGCCACGATGCGGGCGTTGCCGCCGCAGCAGCTTGTCGATGCACTGTCACCGTTGCCGCATCGGGAGTATTCGATTGCC
TCGCTGCCGCAGGATGGTCAGCTCGAACTGCTGGTGCGACAGACCCGGCATGCCGACGGCAGGCTGGGTCTCGCCTCAGG
CTGGCTGACGAGTCATGCGCCGGTGGGCGCGCGCATTGCGCTGCGTATTCGCACCAATCGCAGCTTCCATTCGCCGGCTG
ACGGCCGGCCGCAGGTACTGATTGGCAATGGCACCGGCCTGGCCGGGCTGCGCGGCCACCTGAAGGCGCGTGCGCTGGCG
GGGCGCCATCGCAACTGGCTGTTCTTCGGTGAGCGGTCGGCGCGGCATGATGGATTCTTTGCCGATGAACTCGCGGCCTG
GCAGGCACAAGGTGTGCTGGAGCGGGTCGACCTTGTGTATTCTCGCGACGGCAATGCCTTGCGATATGTGCAGGACGCGC
TCCGGGAGCGCGCACCGGTGCTGCGGGAATGGGTTGAGGCGGGTGCGGCGATTTACGTTTGCGGCAGCCTGCAAGGCATG
GCCGGTGGCGTCAATGACGCGCTGGCCGATGTGCTTGGCGAGGCGGGTTTGCGCCGGCTGACCGAGCAGGGTCGCTATCG
CCGCGACGTCTACTAG

Upstream 100 bases:

>100_bases
GCGGCTTTGACGAACATCTTTCCCCGGCCTTTGCCGCGATGCTGGCGTGAACCTATTCACCGCATGCTGGCTGGCCGCTG
TGGCAGGTGTCTGCGTGGCA

Downstream 100 bases:

>100_bases
ATCCGCCAGCTCCCGTTTATCCGACGTACCCGAGGATGCTGACGAACTGGCATGCACTGCCGGCCAGCACGAACAGGTGC
CAGATGCCATGGAAGTGCTT

Product: flavodoxin/nitric oxide synthase

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MLSPSRAGMAAGVLLAYAGFCGAVFDHHRRRRARVASLAVSGAHQEGATLVAYASQTGFAEQLAMQTATALQGAGMPVHL
LSFEELDARRLARVRQALFVVSTTGEGDAPDSASGFARRLLAGGGHDALRQLRYGVLALGDSSYARFCAFGHALSDWLQR
HHARPLFDLIEVDNGDAGALRHWQNHLSALSGGAEIADWERPRYEHWQLAAREHLNPGSDGAPAFHLVLRPEGAVPGWQA
GDIAEVGPCHDPADIGRLLSHLSMDGAAPVRCDAQTVTLAEALATRLLLPEAHLATMRALPPQQLVDALSPLPHREYSIA
SLPQDGQLELLVRQTRHADGRLGLASGWLTSHAPVGARIALRIRTNRSFHSPADGRPQVLIGNGTGLAGLRGHLKARALA
GRHRNWLFFGERSARHDGFFADELAAWQAQGVLERVDLVYSRDGNALRYVQDALRERAPVLREWVEAGAAIYVCGSLQGM
AGGVNDALADVLGEAGLRRLTEQGRYRRDVY

Sequences:

>Translated_511_residues
MLSPSRAGMAAGVLLAYAGFCGAVFDHHRRRRARVASLAVSGAHQEGATLVAYASQTGFAEQLAMQTATALQGAGMPVHL
LSFEELDARRLARVRQALFVVSTTGEGDAPDSASGFARRLLAGGGHDALRQLRYGVLALGDSSYARFCAFGHALSDWLQR
HHARPLFDLIEVDNGDAGALRHWQNHLSALSGGAEIADWERPRYEHWQLAAREHLNPGSDGAPAFHLVLRPEGAVPGWQA
GDIAEVGPCHDPADIGRLLSHLSMDGAAPVRCDAQTVTLAEALATRLLLPEAHLATMRALPPQQLVDALSPLPHREYSIA
SLPQDGQLELLVRQTRHADGRLGLASGWLTSHAPVGARIALRIRTNRSFHSPADGRPQVLIGNGTGLAGLRGHLKARALA
GRHRNWLFFGERSARHDGFFADELAAWQAQGVLERVDLVYSRDGNALRYVQDALRERAPVLREWVEAGAAIYVCGSLQGM
AGGVNDALADVLGEAGLRRLTEQGRYRRDVY
>Mature_511_residues
MLSPSRAGMAAGVLLAYAGFCGAVFDHHRRRRARVASLAVSGAHQEGATLVAYASQTGFAEQLAMQTATALQGAGMPVHL
LSFEELDARRLARVRQALFVVSTTGEGDAPDSASGFARRLLAGGGHDALRQLRYGVLALGDSSYARFCAFGHALSDWLQR
HHARPLFDLIEVDNGDAGALRHWQNHLSALSGGAEIADWERPRYEHWQLAAREHLNPGSDGAPAFHLVLRPEGAVPGWQA
GDIAEVGPCHDPADIGRLLSHLSMDGAAPVRCDAQTVTLAEALATRLLLPEAHLATMRALPPQQLVDALSPLPHREYSIA
SLPQDGQLELLVRQTRHADGRLGLASGWLTSHAPVGARIALRIRTNRSFHSPADGRPQVLIGNGTGLAGLRGHLKARALA
GRHRNWLFFGERSARHDGFFADELAAWQAQGVLERVDLVYSRDGNALRYVQDALRERAPVLREWVEAGAAIYVCGSLQGM
AGGVNDALADVLGEAGLRRLTEQGRYRRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=238, Percent_Identity=28.9915966386555, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI169790956, Length=261, Percent_Identity=30.2681992337165, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI7657393, Length=240, Percent_Identity=30.8333333333333, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI169790958, Length=261, Percent_Identity=30.2681992337165, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI221316709, Length=238, Percent_Identity=30.672268907563, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI221316705, Length=249, Percent_Identity=30.5220883534137, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI24041029, Length=235, Percent_Identity=31.9148936170213, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI10835173, Length=225, Percent_Identity=28.4444444444444, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI221316707, Length=192, Percent_Identity=32.2916666666667, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI40254422, Length=225, Percent_Identity=31.1111111111111, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1789123, Length=542, Percent_Identity=29.3357933579336, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17566446, Length=224, Percent_Identity=33.0357142857143, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554134, Length=224, Percent_Identity=30.3571428571429, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531441, Length=127, Percent_Identity=33.0708661417323, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32563863, Length=127, Percent_Identity=33.0708661417323, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6321832, Length=223, Percent_Identity=29.5964125560538, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321143, Length=233, Percent_Identity=24.4635193133047, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17137192, Length=238, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583543, Length=241, Percent_Identity=31.5352697095436, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78706876, Length=241, Percent_Identity=31.5352697095436, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582192, Length=238, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78706872, Length=241, Percent_Identity=31.5352697095436, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 55177; Mature: 55177

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPSRAGMAAGVLLAYAGFCGAVFDHHRRRRARVASLAVSGAHQEGATLVAYASQTGFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHH
EQLAMQTATALQGAGMPVHLLSFEELDARRLARVRQALFVVSTTGEGDAPDSASGFARRL
HHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHH
LAGGGHDALRQLRYGVLALGDSSYARFCAFGHALSDWLQRHHARPLFDLIEVDNGDAGAL
HCCCCHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHH
RHWQNHLSALSGGAEIADWERPRYEHWQLAAREHLNPGSDGAPAFHLVLRPEGAVPGWQA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCC
GDIAEVGPCHDPADIGRLLSHLSMDGAAPVRCDAQTVTLAEALATRLLLPEAHLATMRAL
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHCC
PPQQLVDALSPLPHREYSIASLPQDGQLELLVRQTRHADGRLGLASGWLTSHAPVGARIA
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEE
LRIRTNRSFHSPADGRPQVLIGNGTGLAGLRGHLKARALAGRHRNWLFFGERSARHDGFF
EEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCH
ADELAAWQAQGVLERVDLVYSRDGNALRYVQDALRERAPVLREWVEAGAAIYVCGSLQGM
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
AGGVNDALADVLGEAGLRRLTEQGRYRRDVY
CCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MLSPSRAGMAAGVLLAYAGFCGAVFDHHRRRRARVASLAVSGAHQEGATLVAYASQTGFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHH
EQLAMQTATALQGAGMPVHLLSFEELDARRLARVRQALFVVSTTGEGDAPDSASGFARRL
HHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHH
LAGGGHDALRQLRYGVLALGDSSYARFCAFGHALSDWLQRHHARPLFDLIEVDNGDAGAL
HCCCCHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHH
RHWQNHLSALSGGAEIADWERPRYEHWQLAAREHLNPGSDGAPAFHLVLRPEGAVPGWQA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCC
GDIAEVGPCHDPADIGRLLSHLSMDGAAPVRCDAQTVTLAEALATRLLLPEAHLATMRAL
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHCC
PPQQLVDALSPLPHREYSIASLPQDGQLELLVRQTRHADGRLGLASGWLTSHAPVGARIA
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEE
LRIRTNRSFHSPADGRPQVLIGNGTGLAGLRGHLKARALAGRHRNWLFFGERSARHDGFF
EEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCH
ADELAAWQAQGVLERVDLVYSRDGNALRYVQDALRERAPVLREWVEAGAAIYVCGSLQGM
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
AGGVNDALADVLGEAGLRRLTEQGRYRRDVY
CCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA