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The map label for this gene is paaF [C]

Identifier: 73538695

GI number: 73538695

Start: 1585836

End: 1586669

Strand: Reverse

Name: paaF [C]

Synonym: Reut_B4870

Alternate gene names: 73538695

Gene position: 1586669-1585836 (Counterclockwise)

Preceding gene: 73538696

Following gene: 73538694

Centisome position: 58.2

GC content: 63.79

Gene sequence:

>834_bases
ATGGCCAATTACGAAGGACGCTGGAAGACCGTAAAGGTCAGCGTGGAAGAGGGCATTGCATGGGTAATGTTTAACCGCCC
CGAAAAGCGCAACGCGATGAGCCCGACCCTGAACAGCGAGATGATCCAGGTACTCGAAGCGCTCGAACTCGACGCCGATG
CCAGGGTGGTGGTGCTGACGGGCGCCGGCGACGCATGGACCGCCGGCATGGATCTCAAGGAGTATTTCCGCGAAGTCGAC
GCCGGCCCCGAGATCCTGCAGGAGAAGATCCGCCGCGACGCCTGCCAGTGGCAATGGAAGCTGTTGCGCATGTATGCGAA
GCCGACCATCGCGATGGTCAACGGTTGGTGCTTCGGCGGCGGCTTTTCGCCTCTGGTCGCATGCGACCTCGCGATTGCCG
CCGATGAGGCCGTGTTCGGCCTGTCGGAAATCAACTGGGGCATCCCGCCGGGCAACCTGGTCAGCAAGGCGATGGCCGAT
ACGGTCGGACATCGCCAGGCGCTGCACTACATCATGACCGGCGACACCTTCACGGGCCAGCAGGCGGCCGCGATGGGCCT
GGTCAACAAGAGCGTGCCGCGCTCGCAATTGCGTGAGCACGTGCTGGAACTGGCCGGCAAGCTGCTGGAGAAGAACCCCG
TCGTGCTGCGCGCCGCCAAGCACGGCTTCAAGCGCAGCCGCGAACTGACCTGGGAGCAGAACGAGGACTACCTGTACGCC
AAGCTCGACCAGGCGCAACTGCGCGACCCCGAGCACGGACGCGAACAGGGCCTCAAGCAGTTCCTCGACGACAAGTCGAT
CAAGCCGGGCCTGCAAGCCTACAAGCGCGCCTGA

Upstream 100 bases:

>100_bases
GGTTTTCACGAGGTCGGTCGGATTTCGCTTGCAATATCAGGTAACCTGATATTAAATTGATTCCACGACGAACCGCCGAC
CAGTCATCAAGGAGACGAAC

Downstream 100 bases:

>100_bases
GGCCGCGGGGCGCCGTAGAGCGCCCCTTGACAAGCCCCCCGACAGGAAGCGGGCTTCGCAGCGTGCGGGGCCCGCCTGGA
GACATGACTTCATGAACGCG

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MANYEGRWKTVKVSVEEGIAWVMFNRPEKRNAMSPTLNSEMIQVLEALELDADARVVVLTGAGDAWTAGMDLKEYFREVD
AGPEILQEKIRRDACQWQWKLLRMYAKPTIAMVNGWCFGGGFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMAD
TVGHRQALHYIMTGDTFTGQQAAAMGLVNKSVPRSQLREHVLELAGKLLEKNPVVLRAAKHGFKRSRELTWEQNEDYLYA
KLDQAQLRDPEHGREQGLKQFLDDKSIKPGLQAYKRA

Sequences:

>Translated_277_residues
MANYEGRWKTVKVSVEEGIAWVMFNRPEKRNAMSPTLNSEMIQVLEALELDADARVVVLTGAGDAWTAGMDLKEYFREVD
AGPEILQEKIRRDACQWQWKLLRMYAKPTIAMVNGWCFGGGFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMAD
TVGHRQALHYIMTGDTFTGQQAAAMGLVNKSVPRSQLREHVLELAGKLLEKNPVVLRAAKHGFKRSRELTWEQNEDYLYA
KLDQAQLRDPEHGREQGLKQFLDDKSIKPGLQAYKRA
>Mature_276_residues
ANYEGRWKTVKVSVEEGIAWVMFNRPEKRNAMSPTLNSEMIQVLEALELDADARVVVLTGAGDAWTAGMDLKEYFREVDA
GPEILQEKIRRDACQWQWKLLRMYAKPTIAMVNGWCFGGGFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADT
VGHRQALHYIMTGDTFTGQQAAAMGLVNKSVPRSQLREHVLELAGKLLEKNPVVLRAAKHGFKRSRELTWEQNEDYLYAK
LDQAQLRDPEHGREQGLKQFLDDKSIKPGLQAYKRA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=29.5546558704453, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI70995211, Length=246, Percent_Identity=28.0487804878049, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI31542718, Length=261, Percent_Identity=29.8850574712644, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI45643119, Length=229, Percent_Identity=31.0043668122271, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI260275230, Length=229, Percent_Identity=31.0043668122271, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI260274832, Length=229, Percent_Identity=31.0043668122271, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI25453481, Length=227, Percent_Identity=23.3480176211454, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI51538573, Length=227, Percent_Identity=23.3480176211454, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI4757966, Length=227, Percent_Identity=23.3480176211454, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538581, Length=227, Percent_Identity=23.3480176211454, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4757968, Length=232, Percent_Identity=24.1379310344828, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI49355787, Length=232, Percent_Identity=24.1379310344828, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=228, Percent_Identity=23.2456140350877, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221136756, Length=228, Percent_Identity=23.2456140350877, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221136753, Length=228, Percent_Identity=23.2456140350877, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI62530384, Length=206, Percent_Identity=29.6116504854369, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=27.1317829457364, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI68989263, Length=173, Percent_Identity=26.0115606936416, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI157694516, Length=232, Percent_Identity=26.2931034482759, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI213417737, Length=232, Percent_Identity=26.2931034482759, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=31.1284046692607, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788597, Length=210, Percent_Identity=35.2380952380952, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI221142681, Length=228, Percent_Identity=30.2631578947368, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=24.1635687732342, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=29.9492385786802, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790281, Length=208, Percent_Identity=28.8461538461538, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=229, Percent_Identity=30.5676855895196, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=28.1746031746032, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=31.4009661835749, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=246, Percent_Identity=25.609756097561, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=247, Percent_Identity=23.0769230769231, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=206, Percent_Identity=26.6990291262136, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=234, Percent_Identity=23.9316239316239, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=25.3968253968254, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129971, Length=268, Percent_Identity=26.865671641791, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653477, Length=268, Percent_Identity=26.865671641791, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21357171, Length=200, Percent_Identity=29, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=28.0155642023346, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24650670, Length=184, Percent_Identity=29.3478260869565, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19920382, Length=244, Percent_Identity=26.6393442622951, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=25.7692307692308, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583165, Length=280, Percent_Identity=23.2142857142857, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 31062; Mature: 30931

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANYEGRWKTVKVSVEEGIAWVMFNRPEKRNAMSPTLNSEMIQVLEALELDADARVVVLT
CCCCCCCEEEEEEEHHHCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
GAGDAWTAGMDLKEYFREVDAGPEILQEKIRRDACQWQWKLLRMYAKPTIAMVNGWCFGG
CCCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECC
GFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADTVGHRQALHYIMTGDTFTGQ
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
QAAAMGLVNKSVPRSQLREHVLELAGKLLEKNPVVLRAAKHGFKRSRELTWEQNEDYLYA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCEEEE
KLDQAQLRDPEHGREQGLKQFLDDKSIKPGLQAYKRA
ECCHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
ANYEGRWKTVKVSVEEGIAWVMFNRPEKRNAMSPTLNSEMIQVLEALELDADARVVVLT
CCCCCCEEEEEEEHHHCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
GAGDAWTAGMDLKEYFREVDAGPEILQEKIRRDACQWQWKLLRMYAKPTIAMVNGWCFGG
CCCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECC
GFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADTVGHRQALHYIMTGDTFTGQ
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCH
QAAAMGLVNKSVPRSQLREHVLELAGKLLEKNPVVLRAAKHGFKRSRELTWEQNEDYLYA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCEEEE
KLDQAQLRDPEHGREQGLKQFLDDKSIKPGLQAYKRA
ECCHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA