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The map label for this gene is ntaB [H]

Identifier: 73538660

GI number: 73538660

Start: 1543573

End: 1544133

Strand: Reverse

Name: ntaB [H]

Synonym: Reut_B4835

Alternate gene names: 73538660

Gene position: 1544133-1543573 (Counterclockwise)

Preceding gene: 73538661

Following gene: 73538656

Centisome position: 56.64

GC content: 56.68

Gene sequence:

>561_bases
ATGGCGCACCGGTCGACGCGCCCGCTGAAAGGAAAAGCGATGGAACATATTGATGGTCTGACTCTTCGCAAGGCGCTGGG
TGCTTTCCCCACCGGGGTGACCGTGATGACCACTTTGGACACCACTGGAAGGGCATGTGGCATCACCGTCAACTCGTTCA
GTTCTGTCTCGCTGACTCCGCCCCTGATCTTATGGAGCCAATCTCTTTCATCAAGGAGCTACCCCGTGTTTCGGGATGCC
GAGCGGTTCGTCGTAAATATCCTCTCTGAGCACCAAATACAGGTATCCAGGCAATTTTCGACAGCCAGCGACGATAAGTT
CCGCAATATCGTCAGCCGCCCGGGTTTGGGGGGTGTTCCAATTATCAGTTCATGCGCTGCCAACCTGGAATGCGTGAAGA
TTTCCGCTCATCCGGAAGGTGACCATGTCGTTTATATCGGACGGGTTGTCCGCCTGGAACGGTCCGCGAACAGGCCGCTC
GTCTTCGGCAATGGACGCTACCTTGTCGCACGCGCCCATCAGCAGGATGAGCTCGATGCGGTGCTCCCATTGGAGGCCTA
G

Upstream 100 bases:

>100_bases
GCAGACGATCTTCCCTGGCGCGATTGTCCTAATGGAACGACGGACTGGTTCTATCTCTCGCCTGCAGGATCTGATGTACT
AGGGACACACAACAGGCTGC

Downstream 100 bases:

>100_bases
GGCTGGTGGTGTCTGCAGCATGTCACAGGCAGTTCGGGATCGCTGGCAGTGGGCCGATGACAGTGTCCGGACTCGCAAGC
CACCCCGCAGCTGCTTGCTT

Product: flavin reductase-like protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MAHRSTRPLKGKAMEHIDGLTLRKALGAFPTGVTVMTTLDTTGRACGITVNSFSSVSLTPPLILWSQSLSSRSYPVFRDA
ERFVVNILSEHQIQVSRQFSTASDDKFRNIVSRPGLGGVPIISSCAANLECVKISAHPEGDHVVYIGRVVRLERSANRPL
VFGNGRYLVARAHQQDELDAVLPLEA

Sequences:

>Translated_186_residues
MAHRSTRPLKGKAMEHIDGLTLRKALGAFPTGVTVMTTLDTTGRACGITVNSFSSVSLTPPLILWSQSLSSRSYPVFRDA
ERFVVNILSEHQIQVSRQFSTASDDKFRNIVSRPGLGGVPIISSCAANLECVKISAHPEGDHVVYIGRVVRLERSANRPL
VFGNGRYLVARAHQQDELDAVLPLEA
>Mature_185_residues
AHRSTRPLKGKAMEHIDGLTLRKALGAFPTGVTVMTTLDTTGRACGITVNSFSSVSLTPPLILWSQSLSSRSYPVFRDAE
RFVVNILSEHQIQVSRQFSTASDDKFRNIVSRPGLGGVPIISSCAANLECVKISAHPEGDHVVYIGRVVRLERSANRPLV
FGNGRYLVARAHQQDELDAVLPLEA

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 20296; Mature: 20164

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHRSTRPLKGKAMEHIDGLTLRKALGAFPTGVTVMTTLDTTGRACGITVNSFSSVSLTP
CCCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCC
PLILWSQSLSSRSYPVFRDAERFVVNILSEHQIQVSRQFSTASDDKFRNIVSRPGLGGVP
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCCCCC
IISSCAANLECVKISAHPEGDHVVYIGRVVRLERSANRPLVFGNGRYLVARAHQQDELDA
HHHHHCCCCEEEEEECCCCCCEEEEEEEEEEEECCCCCCEEEECCEEEEEECCCCCCCCE
VLPLEA
EECCCC
>Mature Secondary Structure 
AHRSTRPLKGKAMEHIDGLTLRKALGAFPTGVTVMTTLDTTGRACGITVNSFSSVSLTP
CCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCC
PLILWSQSLSSRSYPVFRDAERFVVNILSEHQIQVSRQFSTASDDKFRNIVSRPGLGGVP
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCCCCC
IISSCAANLECVKISAHPEGDHVVYIGRVVRLERSANRPLVFGNGRYLVARAHQQDELDA
HHHHHCCCCEEEEEECCCCCCEEEEEEEEEEEECCCCCCEEEECCEEEEEECCCCCCCCE
VLPLEA
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]