Click here to switch to the map view.

The map label for this gene is opuAB [H]

Identifier: 73538635

GI number: 73538635

Start: 1510017

End: 1510913

Strand: Reverse

Name: opuAB [H]

Synonym: Reut_B4810

Alternate gene names: 73538635

Gene position: 1510913-1510017 (Counterclockwise)

Preceding gene: 73538636

Following gene: 73538634

Centisome position: 55.42

GC content: 64.21

Gene sequence:

>897_bases
ATGTCTGAATACCTTCCGCTCGGCCACTGGGTCGACCAGTCTGTCCACTATCTTCTCGATCACGACGCAGCAACGTTCGA
CACGGTCGGCCGCGCGATCGAGGGGCTCGCCGCACTGATCGAGCGCAGCCTGCAGGCAGTGCCGATGTGGCTGATGATGG
CGATCTTCATCGGCATTGGACTCTGGCGCGTCGGCTGGCGCTTCGCGCTCTTCACGGCCGCGTCGCTGTCCGTGATCTTC
GCCACGGGATTCTGGGACCAGACGGTCATTACGCTGGGCCTGACGCTGTCATCGACCATCATCAGTCTGATCCTGGGCGT
TCCGCTCGGCATCTGGGCGGCGAAGAGCAGAGTGGTGGCGGCCGTCGTGCGCCCGATCCTCGACCTGATGCAGACCATGC
CGGCGTTCGTCTACCTGATTCCCGCGGCGATGCTGTTCGGCCTCGGCCGCGTGCCGGGCATCCTGTCGACCGTGATCTTT
GCGATGCCGCCTGCTGTGCGCCTGACAAGTCTTGGGATCCGGCAGGTGAACATGGAGATCGTCGAGGCCGGCCATGCCTT
CGGCTGTACGCCATGGCAACTGCTGTACAAGGTGCAGTTCCCGAACGCGCTGCCGTCTATCATGCAAGGCGTCAACCAGA
CGATCATGATGGCGCTGTCGATGGTGATCATCGCCTCGATGGTCGGCGCTGGCGGCCTGGGCAACGAGGTGCTCGCGAGC
ATCCAGCGCCTGGACATCGGCCTGGGCTTCGAAAGCGGCCTGTCTGTCGTGCTGCTGGCAATTATCCTGGACCGCATTAC
CGAGAGCTTCGGCCGCGCGCCAGGCACCGTGAAGGCACCGCTTCTCTCGGGGCTCAGGCAACTGTTCAGCGCCAAGGCCA
CGACGGCCCGGGCCTGA

Upstream 100 bases:

>100_bases
AGCGCGAATGCGCTGCCGGTCGTCGACGAGTACGGCTGCTACTGTGGCTCGGTCGACCGCGCCGTCATCCTGAAAGCCAT
CACGCACCTGCGAGGTCACC

Downstream 100 bases:

>100_bases
TGCCGGCTCATCTGTCCGCCAGGAGCGCGCTGTGAGGTCCGTTGCTGCCGCGCTGCCTGACAGCGCTTCATCGCCGATGT
CTTCGCTCGCGCATTTCGGC

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSEYLPLGHWVDQSVHYLLDHDAATFDTVGRAIEGLAALIERSLQAVPMWLMMAIFIGIGLWRVGWRFALFTAASLSVIF
ATGFWDQTVITLGLTLSSTIISLILGVPLGIWAAKSRVVAAVVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIF
AMPPAVRLTSLGIRQVNMEIVEAGHAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNEVLAS
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGTVKAPLLSGLRQLFSAKATTARA

Sequences:

>Translated_298_residues
MSEYLPLGHWVDQSVHYLLDHDAATFDTVGRAIEGLAALIERSLQAVPMWLMMAIFIGIGLWRVGWRFALFTAASLSVIF
ATGFWDQTVITLGLTLSSTIISLILGVPLGIWAAKSRVVAAVVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIF
AMPPAVRLTSLGIRQVNMEIVEAGHAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNEVLAS
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGTVKAPLLSGLRQLFSAKATTARA
>Mature_297_residues
SEYLPLGHWVDQSVHYLLDHDAATFDTVGRAIEGLAALIERSLQAVPMWLMMAIFIGIGLWRVGWRFALFTAASLSVIFA
TGFWDQTVITLGLTLSSTIISLILGVPLGIWAAKSRVVAAVVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFA
MPPAVRLTSLGIRQVNMEIVEAGHAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNEVLASI
QRLDIGLGFESGLSVVLLAIILDRITESFGRAPGTVKAPLLSGLRQLFSAKATTARA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=268, Percent_Identity=45.8955223880597, Blast_Score=223, Evalue=9e-60,
Organism=Escherichia coli, GI1788451, Length=201, Percent_Identity=28.8557213930348, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788449, Length=180, Percent_Identity=28.3333333333333, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31979; Mature: 31847

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYLPLGHWVDQSVHYLLDHDAATFDTVGRAIEGLAALIERSLQAVPMWLMMAIFIGIG
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWRVGWRFALFTAASLSVIFATGFWDQTVITLGLTLSSTIISLILGVPLGIWAAKSRVVA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AVVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFAMPPAVRLTSLGIRQVNMEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VEAGHAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNEVLAS
HHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGTVKAPLLSGLRQLFSAKATTARA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEYLPLGHWVDQSVHYLLDHDAATFDTVGRAIEGLAALIERSLQAVPMWLMMAIFIGIG
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWRVGWRFALFTAASLSVIFATGFWDQTVITLGLTLSSTIISLILGVPLGIWAAKSRVVA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AVVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFAMPPAVRLTSLGIRQVNMEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VEAGHAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNEVLAS
HHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGTVKAPLLSGLRQLFSAKATTARA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]