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The map label for this gene is pntA [H]

Identifier: 73538493

GI number: 73538493

Start: 1361362

End: 1362918

Strand: Reverse

Name: pntA [H]

Synonym: Reut_B4667

Alternate gene names: 73538493

Gene position: 1362918-1361362 (Counterclockwise)

Preceding gene: 73538494

Following gene: 73538492

Centisome position: 49.99

GC content: 67.44

Gene sequence:

>1557_bases
ATGCCAATGACAATTGGTGTCCCCCGGGAGGTCCATCCCGGGGAGCGGCGCGTTGCCGCGACGCCCGATTCGGTCAGGGA
GCTGACCAAGCTCGGTTATGAGGTGGCAGTGGAATCCGGCGCAGGCCAGCAGGCCTCGTTCTCGGACGAAGCGTATCGTG
CCGCCGGCGCAGCCATTGTCGATGCCGCCAGCCTGTGGGCGTCGGTCGACGTCGTCATCAAGGTGCGCCCGCCGCAGGTC
CACCCGAGCCTCGGCGTGGAAGAGGCGGCGCTGCTCAAGAAGCACGCCACGCTGATCAGCTTTGTGTGGCCCGCGCAGCA
GTTGACGCTGCTGGAGCGGCTGTCGCAACGCGGCGCCACGGTGCTCGCGATGGATTGCGTGCCGCGCATTTCACGCGCGC
AGAAGCTCGATGCGCTCAGCTCGATGGCCAATATGGCGGGCTATCGCGCAGTGATCGAAGCCGCGCATGCCTTCGGCCGA
CCGTTCGCAGGCCAGATCACGGCCGCGGGCAAGATTCCGCCCGCGCGCGTGCTGGTGATCGGCGCTGGCGTGGCTGGCCT
TGCCGCCATTGGCGCAGCGCGCAGCCTCGGTGCCGTGGTACGCGCGTTCGACACGCGACCCGTGGTGCGCCAGCAGATCG
AAAGTCTCGGCGCCGAATTCCTGACGGTGGAAATCGAGGAAGACGGCAGCGGCAGTGGCGGCTACGCGAAGGAGATGAGC
CCGGCGTTCATCGAGGCCGAGATGAAGCTGTTCGCCGAGCAGGCGCGCGAAGTGGACATCATCATCACGACCGCGCTGAT
TCCCGGCAAGCCCGCACCCCGCTTGCTGGAGGCCGACACGGTGGCGCTGATGCGTTCCGGCAGCGTGGTGGTCGATCTCG
CCGCGGAGCAGGGCGGCAATTGCGTGCTGACCGTGCCTGGTGAATCGGTGACGCACAACGGGGTCACGGTGGTCGGCTAC
ACCGACCTGCCCAGCCGCATGGCCGCACAGTCGAGCCAGCTCTACGCCACCAACATCCGGCACCTGCTGACGGAGCTGAC
GCCCGCCAAGGACGGCCAGCTCGTTGTCGATATGGATGACGAGATGATCCGTGGCGCCACGGTGCTGCACCAGGGCGACG
TGACCTGGCCGCCGCCACCGCTGAAGACCTTGCCGCCACCGGCACCTGCAGCGGAAGTGCAGGCGCCCTCCGAACCCGTC
GCGCCACCGAGCCGCACGGGCACGACGCTGGCGGCACTGGCTATCGCCGTCATTGCGTTGCTGGCGCTCGGTGCCGTGGC
ACCACCAGCGTTCATGGCGCACTTCACGGTGTTCGTGCTCGCCATTTTTGTCGGCTACCAGGTCGTGTGGAACGTGACGG
CAGCCCTGCACACGCCGCTGATGAGCGTGACCAATGCGATCAGCGGGATCATCGTGGTCGGCGCGCTCGTGCAGCTCGGC
AAGCCGTCGCTCGTTACCGCCATCGTCGCCGGCGCGGCGGTGCTGATCGCCACCATCAACATCGCAGGCGGCTTCCTCGT
GACGCAGCGCATGCTGAAGATGTTCCAGCGGGACTGA

Upstream 100 bases:

>100_bases
TCGATGAAGGATGGCGCGGTGGTGGAGGTGAGCATCGAAGGCATCGGCTCGCTGGTGAACCACGTTCGCGGGTAATCGCG
CAATAATCAAAGGGAAAGCC

Downstream 100 bases:

>100_bases
GGAGACAGACATGCCTTCGGGAATCAGCAGTATTGCTTACCTGGGCGCGAGCGCGCTCTTCATCCTCAGCCTGAGCGGGC
TGAGCCATCCGGCCAGCGCG

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MPMTIGVPREVHPGERRVAATPDSVRELTKLGYEVAVESGAGQQASFSDEAYRAAGAAIVDAASLWASVDVVIKVRPPQV
HPSLGVEEAALLKKHATLISFVWPAQQLTLLERLSQRGATVLAMDCVPRISRAQKLDALSSMANMAGYRAVIEAAHAFGR
PFAGQITAAGKIPPARVLVIGAGVAGLAAIGAARSLGAVVRAFDTRPVVRQQIESLGAEFLTVEIEEDGSGSGGYAKEMS
PAFIEAEMKLFAEQAREVDIIITTALIPGKPAPRLLEADTVALMRSGSVVVDLAAEQGGNCVLTVPGESVTHNGVTVVGY
TDLPSRMAAQSSQLYATNIRHLLTELTPAKDGQLVVDMDDEMIRGATVLHQGDVTWPPPPLKTLPPPAPAAEVQAPSEPV
APPSRTGTTLAALAIAVIALLALGAVAPPAFMAHFTVFVLAIFVGYQVVWNVTAALHTPLMSVTNAISGIIVVGALVQLG
KPSLVTAIVAGAAVLIATINIAGGFLVTQRMLKMFQRD

Sequences:

>Translated_518_residues
MPMTIGVPREVHPGERRVAATPDSVRELTKLGYEVAVESGAGQQASFSDEAYRAAGAAIVDAASLWASVDVVIKVRPPQV
HPSLGVEEAALLKKHATLISFVWPAQQLTLLERLSQRGATVLAMDCVPRISRAQKLDALSSMANMAGYRAVIEAAHAFGR
PFAGQITAAGKIPPARVLVIGAGVAGLAAIGAARSLGAVVRAFDTRPVVRQQIESLGAEFLTVEIEEDGSGSGGYAKEMS
PAFIEAEMKLFAEQAREVDIIITTALIPGKPAPRLLEADTVALMRSGSVVVDLAAEQGGNCVLTVPGESVTHNGVTVVGY
TDLPSRMAAQSSQLYATNIRHLLTELTPAKDGQLVVDMDDEMIRGATVLHQGDVTWPPPPLKTLPPPAPAAEVQAPSEPV
APPSRTGTTLAALAIAVIALLALGAVAPPAFMAHFTVFVLAIFVGYQVVWNVTAALHTPLMSVTNAISGIIVVGALVQLG
KPSLVTAIVAGAAVLIATINIAGGFLVTQRMLKMFQRD
>Mature_517_residues
PMTIGVPREVHPGERRVAATPDSVRELTKLGYEVAVESGAGQQASFSDEAYRAAGAAIVDAASLWASVDVVIKVRPPQVH
PSLGVEEAALLKKHATLISFVWPAQQLTLLERLSQRGATVLAMDCVPRISRAQKLDALSSMANMAGYRAVIEAAHAFGRP
FAGQITAAGKIPPARVLVIGAGVAGLAAIGAARSLGAVVRAFDTRPVVRQQIESLGAEFLTVEIEEDGSGSGGYAKEMSP
AFIEAEMKLFAEQAREVDIIITTALIPGKPAPRLLEADTVALMRSGSVVVDLAAEQGGNCVLTVPGESVTHNGVTVVGYT
DLPSRMAAQSSQLYATNIRHLLTELTPAKDGQLVVDMDDEMIRGATVLHQGDVTWPPPPLKTLPPPAPAAEVQAPSEPVA
PPSRTGTTLAALAIAVIALLALGAVAPPAFMAHFTVFVLAIFVGYQVVWNVTAALHTPLMSVTNAISGIIVVGALVQLGK
PSLVTAIVAGAAVLIATINIAGGFLVTQRMLKMFQRD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=533, Percent_Identity=54.2213883677298, Blast_Score=542, Evalue=1e-154,
Organism=Homo sapiens, GI122939153, Length=533, Percent_Identity=54.2213883677298, Blast_Score=542, Evalue=1e-154,
Organism=Escherichia coli, GI1787887, Length=521, Percent_Identity=59.1170825335892, Blast_Score=600, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI17550456, Length=525, Percent_Identity=52.3809523809524, Blast_Score=493, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54062; Mature: 53931

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMTIGVPREVHPGERRVAATPDSVRELTKLGYEVAVESGAGQQASFSDEAYRAAGAAIV
CCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHHCHHHH
DAASLWASVDVVIKVRPPQVHPSLGVEEAALLKKHATLISFVWPAQQLTLLERLSQRGAT
HHHHHHEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCE
VLAMDCVPRISRAQKLDALSSMANMAGYRAVIEAAHAFGRPFAGQITAAGKIPPARVLVI
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEE
GAGVAGLAAIGAARSLGAVVRAFDTRPVVRQQIESLGAEFLTVEIEEDGSGSGGYAKEMS
ECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHCCC
PAFIEAEMKLFAEQAREVDIIITTALIPGKPAPRLLEADTVALMRSGSVVVDLAAEQGGN
HHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
CVLTVPGESVTHNGVTVVGYTDLPSRMAAQSSQLYATNIRHLLTELTPAKDGQLVVDMDD
EEEECCCCCEECCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCH
EMIRGATVLHQGDVTWPPPPLKTLPPPAPAAEVQAPSEPVAPPSRTGTTLAALAIAVIAL
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LALGAVAPPAFMAHFTVFVLAIFVGYQVVWNVTAALHTPLMSVTNAISGIIVVGALVQLG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KPSLVTAIVAGAAVLIATINIAGGFLVTQRMLKMFQRD
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PMTIGVPREVHPGERRVAATPDSVRELTKLGYEVAVESGAGQQASFSDEAYRAAGAAIV
CCCCCCCCCCCCCCCEEECCCHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHHCHHHH
DAASLWASVDVVIKVRPPQVHPSLGVEEAALLKKHATLISFVWPAQQLTLLERLSQRGAT
HHHHHHEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCE
VLAMDCVPRISRAQKLDALSSMANMAGYRAVIEAAHAFGRPFAGQITAAGKIPPARVLVI
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEE
GAGVAGLAAIGAARSLGAVVRAFDTRPVVRQQIESLGAEFLTVEIEEDGSGSGGYAKEMS
ECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHCCC
PAFIEAEMKLFAEQAREVDIIITTALIPGKPAPRLLEADTVALMRSGSVVVDLAAEQGGN
HHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
CVLTVPGESVTHNGVTVVGYTDLPSRMAAQSSQLYATNIRHLLTELTPAKDGQLVVDMDD
EEEECCCCCEECCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCH
EMIRGATVLHQGDVTWPPPPLKTLPPPAPAAEVQAPSEPVAPPSRTGTTLAALAIAVIAL
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LALGAVAPPAFMAHFTVFVLAIFVGYQVVWNVTAALHTPLMSVTNAISGIIVVGALVQLG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KPSLVTAIVAGAAVLIATINIAGGFLVTQRMLKMFQRD
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]