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The map label for this gene is pcaK [H]

Identifier: 73538394

GI number: 73538394

Start: 1246050

End: 1247426

Strand: Reverse

Name: pcaK [H]

Synonym: Reut_B4567

Alternate gene names: 73538394

Gene position: 1247426-1246050 (Counterclockwise)

Preceding gene: 73538395

Following gene: 73538391

Centisome position: 45.76

GC content: 64.85

Gene sequence:

>1377_bases
ATGACAGATACGCGCCCCATTGTCGACGTCAAGCGACTGATCGACGAAGAACGATTTTCCCCCTACCAGTGGCTGATCCT
GGCGGTGTGCTTCCTGACGATCGCGGTGGATACCTACGATGCATTCGCCGTCGGCTTCGTTGTGCCGGCACTGATGCAGG
AGTGGCACGTCGGCAAGGACGTGCTGGGCCCGGTGATGAGTGCCTCCATCTTCGGGATGGCCATTGGGGCGCTGCTGGCC
GGACCGCTGTTCGATCGCACCACCCCGAAGACGGTGATGGTCTTCTCGATGCTGGTGTTCGGCATCTGCAGCCTCTGCAG
CATTACGGCCACTTCGCCGTTGTCGCTTGGCCTGTGGCGATTGTTCACTGGGATCGGCATCGGGGCGGCGGTGCCGGGGG
CGACGACGCTGGTGTTCGAATATGCGCCGGCACGGCGCAGTGCGCTGACCGTCAATGCCATGGGTTGCGGCGGCATGCTC
GGCGCCGCGCTGTGCGGTGTCACAGCGGGCGTACTGGTGCCGGGCTATGGGTGGAAAAGCCTGTTCGTGGCCGGCGCCGT
GCTGCCTATCGTTCTGGCACTGGTTGTCCAGTTTTCTCTGCCCGAGCCAATGCGCTTCATGGTGATGCGCGACTGGCCGG
CGCAGCGGATCGCGGCGGTCTTGCGGCGCATCGCGCCAGGGCGTGATTTCGACGGCGTGCGCTTCGTCTTTTCCGAGGAG
CAGGCGACGGACCGCAAGTCAGGGATGGCGGCAATGCTGTCCAGGCCGCTGCGTACCGGCACCATCATGCTGTGGATCAC
GTACTGCGTGGGAACGTTCTCCTACTATCTGCTGATGGGGTGGATGCCAACGCTGGTCCAGGAATCCGGAGCGTCACTGC
GCGAAGCGACGCTTGCTACGAGCCTGCTGTCGCTGGGCGGCATCGTGGGGGCGTTCGGTGTCGGCTGGCTGATGGACCGA
TTCGAGCGGAATGCCGCTGTGGCGGCGGCATTCGTCGTCGGTGGTGTGGCGGTGTGGATGATCGGCCAGCAGTCTGCCGG
CACGGCATTGTTGCCGGCCATTATCTTTATTGCGGGCGTGGGCCTGAACGGCGCGATCTTTTCCATGGGCGGCCTTGCCG
CGGCGTTCTACCCGACGAATGGGCGCTCCGCGGGCATTGCCTGGATGTACGGCATCGGCCGGATCGGCGGGATTCTCGGG
CCGATTGCAGGTGGATTCCTGCTCCGTGCCAATTCAGGCACCGGCTTGTTCTATCTGATCGTGACGGGAGCCGTGCTGAT
GGCGGCTGCGGCGCTGTGGATCAAGCGCCGGGCCAGTGGCGGCCCGGCCGGCGTGAGTGCCATGGCGCCTGTGCGAAGCG
GATCGGAGGCAAGGTAG

Upstream 100 bases:

>100_bases
CAAGGAGAAGCCATCGTGAGGGAACGTCCCAGGTTCGCGTGCATGTAGGACAACACCCGCGCCGCAACGAAAACAAATCC
ATACACAACGGAGGAGACAA

Downstream 100 bases:

>100_bases
TGTCATTGGGCGTTTGCCATGGCCGATGTCGCGCAAACGCCCGTCAGCGCAGTCTGGGTCAGAGCGGCCGGAACGTCGGC
ATTGGCGGCCCGTTGTCCGT

Product: 4-hydroxybenzoate transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTDTRPIVDVKRLIDEERFSPYQWLILAVCFLTIAVDTYDAFAVGFVVPALMQEWHVGKDVLGPVMSASIFGMAIGALLA
GPLFDRTTPKTVMVFSMLVFGICSLCSITATSPLSLGLWRLFTGIGIGAAVPGATTLVFEYAPARRSALTVNAMGCGGML
GAALCGVTAGVLVPGYGWKSLFVAGAVLPIVLALVVQFSLPEPMRFMVMRDWPAQRIAAVLRRIAPGRDFDGVRFVFSEE
QATDRKSGMAAMLSRPLRTGTIMLWITYCVGTFSYYLLMGWMPTLVQESGASLREATLATSLLSLGGIVGAFGVGWLMDR
FERNAAVAAAFVVGGVAVWMIGQQSAGTALLPAIIFIAGVGLNGAIFSMGGLAAAFYPTNGRSAGIAWMYGIGRIGGILG
PIAGGFLLRANSGTGLFYLIVTGAVLMAAAALWIKRRASGGPAGVSAMAPVRSGSEAR

Sequences:

>Translated_458_residues
MTDTRPIVDVKRLIDEERFSPYQWLILAVCFLTIAVDTYDAFAVGFVVPALMQEWHVGKDVLGPVMSASIFGMAIGALLA
GPLFDRTTPKTVMVFSMLVFGICSLCSITATSPLSLGLWRLFTGIGIGAAVPGATTLVFEYAPARRSALTVNAMGCGGML
GAALCGVTAGVLVPGYGWKSLFVAGAVLPIVLALVVQFSLPEPMRFMVMRDWPAQRIAAVLRRIAPGRDFDGVRFVFSEE
QATDRKSGMAAMLSRPLRTGTIMLWITYCVGTFSYYLLMGWMPTLVQESGASLREATLATSLLSLGGIVGAFGVGWLMDR
FERNAAVAAAFVVGGVAVWMIGQQSAGTALLPAIIFIAGVGLNGAIFSMGGLAAAFYPTNGRSAGIAWMYGIGRIGGILG
PIAGGFLLRANSGTGLFYLIVTGAVLMAAAALWIKRRASGGPAGVSAMAPVRSGSEAR
>Mature_457_residues
TDTRPIVDVKRLIDEERFSPYQWLILAVCFLTIAVDTYDAFAVGFVVPALMQEWHVGKDVLGPVMSASIFGMAIGALLAG
PLFDRTTPKTVMVFSMLVFGICSLCSITATSPLSLGLWRLFTGIGIGAAVPGATTLVFEYAPARRSALTVNAMGCGGMLG
AALCGVTAGVLVPGYGWKSLFVAGAVLPIVLALVVQFSLPEPMRFMVMRDWPAQRIAAVLRRIAPGRDFDGVRFVFSEEQ
ATDRKSGMAAMLSRPLRTGTIMLWITYCVGTFSYYLLMGWMPTLVQESGASLREATLATSLLSLGGIVGAFGVGWLMDRF
ERNAAVAAAFVVGGVAVWMIGQQSAGTALLPAIIFIAGVGLNGAIFSMGGLAAAFYPTNGRSAGIAWMYGIGRIGGILGP
IAGGFLLRANSGTGLFYLIVTGAVLMAAAALWIKRRASGGPAGVSAMAPVRSGSEAR

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=438, Percent_Identity=26.027397260274, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI87081723, Length=391, Percent_Identity=29.6675191815857, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082231, Length=439, Percent_Identity=23.2346241457859, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87082159, Length=387, Percent_Identity=20.4134366925065, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786229, Length=200, Percent_Identity=23.5, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=427, Percent_Identity=24.1217798594848, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922874, Length=428, Percent_Identity=22.8971962616822, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48113; Mature: 47981

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTRPIVDVKRLIDEERFSPYQWLILAVCFLTIAVDTYDAFAVGFVVPALMQEWHVGKD
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VLGPVMSASIFGMAIGALLAGPLFDRTTPKTVMVFSMLVFGICSLCSITATSPLSLGLWR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LFTGIGIGAAVPGATTLVFEYAPARRSALTVNAMGCGGMLGAALCGVTAGVLVPGYGWKS
HHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHH
LFVAGAVLPIVLALVVQFSLPEPMRFMVMRDWPAQRIAAVLRRIAPGRDFDGVRFVFSEE
HHHHHHHHHHHHHHHHHHCCCCCCEEHEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCH
QATDRKSGMAAMLSRPLRTGTIMLWITYCVGTFSYYLLMGWMPTLVQESGASLREATLAT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SLLSLGGIVGAFGVGWLMDRFERNAAVAAAFVVGGVAVWMIGQQSAGTALLPAIIFIAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCC
GLNGAIFSMGGLAAAFYPTNGRSAGIAWMYGIGRIGGILGPIAGGFLLRANSGTGLFYLI
CCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHH
VTGAVLMAAAALWIKRRASGGPAGVSAMAPVRSGSEAR
HHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
TDTRPIVDVKRLIDEERFSPYQWLILAVCFLTIAVDTYDAFAVGFVVPALMQEWHVGKD
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VLGPVMSASIFGMAIGALLAGPLFDRTTPKTVMVFSMLVFGICSLCSITATSPLSLGLWR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LFTGIGIGAAVPGATTLVFEYAPARRSALTVNAMGCGGMLGAALCGVTAGVLVPGYGWKS
HHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHH
LFVAGAVLPIVLALVVQFSLPEPMRFMVMRDWPAQRIAAVLRRIAPGRDFDGVRFVFSEE
HHHHHHHHHHHHHHHHHHCCCCCCEEHEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCH
QATDRKSGMAAMLSRPLRTGTIMLWITYCVGTFSYYLLMGWMPTLVQESGASLREATLAT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SLLSLGGIVGAFGVGWLMDRFERNAAVAAAFVVGGVAVWMIGQQSAGTALLPAIIFIAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCC
GLNGAIFSMGGLAAAFYPTNGRSAGIAWMYGIGRIGGILGPIAGGFLLRANSGTGLFYLI
CCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHH
VTGAVLMAAAALWIKRRASGGPAGVSAMAPVRSGSEAR
HHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]