BacMap

Definition	Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence.
Accession	NC_007348
Length	2,726,152

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The map label for this gene is paaF [C]

Identifier: 73538387

GI number: 73538387

Start: 1238755

End: 1239525

Strand: Reverse

Name: paaF [C]

Synonym: Reut_B4560

Alternate gene names: 73538387

Gene position: 1239525-1238755 (Counterclockwise)

Preceding gene: 73538388

Following gene: 73538386

Centisome position: 45.47

GC content: 67.44

Gene sequence:

>771_bases
ATGAGCCTGCAATCCCGTATCGAAGGCGCCGTCGGCTGGATGACCATGGACCGCCCGGCGGCCATGAACAGCCTGAACCG
CGAGATGGCTGCGGCCATGACCGCGCAGCTCAAGGCCTGGCGCGAGGATCCGCAAGTCCGAGCAGTGGTGCTGACCGGCA
ACGGCCGCGCGTTTTGCGCGGGCGCCGACCTCAAGGAAGTGGCCGAGCCCGTCGTGACGGGAGAACCCGATTTCCTCGAT
GTGGTCGTCGGCTTCGTCGACACGCTGCGCGTGTTTCCGAAGCCCGTCATCGCCGCCGTCAACGGGCTGGCGCTTGCTGG
CGGGCTGGAAACCGTGATGGCCTGCGACCTTGTGCTCGCCGCGGAATCCGCGAAGCTAGGCGATGCCCACTCGGACTTCG
GCGTCTTTCCCGGCGCAGGCGGCGCCGCGGTGCTGCCGCGCAAGATTCCCGCCAACGTGGCGCGCTGGCTGCTGTTCACG
GGCGCCACGTTCACCGCTGCGCAGATGGAGCGGTACGGCCTGGTCAACGAGGTGGTGGCCGATGCCGAACTGCTCGCCCG
TGCGCAAGCCGTCGCGGAACTGCTGGCCACGAAGAGCCCCTTGGTGCTGGCGCGCATGAAACGCGTGGTGGCCGAAGCCG
CCGACAAGTCGCTGGCCGATGCCCTGCGGCATGAACTGCTCGAACTGCGCAACCACCAGCGCTCCCATGACATGAAAGAA
GGCCTGACAGCCTTCGCCGAAAAGCGGCAGCCGGTCTTCAAGGGCTGCTGA

Upstream 100 bases:

>100_bases
GCGCTGAAGCAGCGCGGCGGCCAGCGCGGTCTCGCGAGCCTGTGCCTTGGCGGCGGTGAGGCCGTGGCAGTCGGCGTGGA
ACTTTGCTGAGGGATATGCC

Downstream 100 bases:

>100_bases
CGCGAGACGAGGAATCCGATGGACCTGTTCTACTCCGACGAACACAAGGCGTTCCGGCAGGAAGTGCGCGATTTCATCGC
CGCCAACCTGCCTGACGACA

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSLQSRIEGAVGWMTMDRPAAMNSLNREMAAAMTAQLKAWREDPQVRAVVLTGNGRAFCAGADLKEVAEPVVTGEPDFLD
VVVGFVDTLRVFPKPVIAAVNGLALAGGLETVMACDLVLAAESAKLGDAHSDFGVFPGAGGAAVLPRKIPANVARWLLFT
GATFTAAQMERYGLVNEVVADAELLARAQAVAELLATKSPLVLARMKRVVAEAADKSLADALRHELLELRNHQRSHDMKE
GLTAFAEKRQPVFKGC

Sequences:

>Translated_256_residues
MSLQSRIEGAVGWMTMDRPAAMNSLNREMAAAMTAQLKAWREDPQVRAVVLTGNGRAFCAGADLKEVAEPVVTGEPDFLD
VVVGFVDTLRVFPKPVIAAVNGLALAGGLETVMACDLVLAAESAKLGDAHSDFGVFPGAGGAAVLPRKIPANVARWLLFT
GATFTAAQMERYGLVNEVVADAELLARAQAVAELLATKSPLVLARMKRVVAEAADKSLADALRHELLELRNHQRSHDMKE
GLTAFAEKRQPVFKGC
>Mature_255_residues
SLQSRIEGAVGWMTMDRPAAMNSLNREMAAAMTAQLKAWREDPQVRAVVLTGNGRAFCAGADLKEVAEPVVTGEPDFLDV
VVGFVDTLRVFPKPVIAAVNGLALAGGLETVMACDLVLAAESAKLGDAHSDFGVFPGAGGAAVLPRKIPANVARWLLFTG
ATFTAAQMERYGLVNEVVADAELLARAQAVAELLATKSPLVLARMKRVVAEAADKSLADALRHELLELRNHQRSHDMKEG
LTAFAEKRQPVFKGC

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=35.655737704918, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI31542718, Length=241, Percent_Identity=27.8008298755187, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI4502327, Length=247, Percent_Identity=29.9595141700405, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594469, Length=203, Percent_Identity=30.0492610837438, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI37594471, Length=203, Percent_Identity=30.0492610837438, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI70995211, Length=248, Percent_Identity=28.2258064516129, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI150378541, Length=248, Percent_Identity=29.4354838709677, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI25453481, Length=236, Percent_Identity=26.271186440678, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI51538573, Length=236, Percent_Identity=26.271186440678, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4757966, Length=197, Percent_Identity=27.4111675126904, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538581, Length=197, Percent_Identity=27.4111675126904, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4757968, Length=236, Percent_Identity=25.8474576271186, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI49355787, Length=236, Percent_Identity=25.8474576271186, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=29.2134831460674, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI68989263, Length=223, Percent_Identity=26.9058295964126, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260274832, Length=196, Percent_Identity=31.1224489795918, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI45643119, Length=196, Percent_Identity=31.1224489795918, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI260275230, Length=196, Percent_Identity=31.1224489795918, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221307494, Length=192, Percent_Identity=27.6041666666667, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221136753, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=35.7429718875502, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=35.4961832061069, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=37.2549019607843, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=30.8, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI87082183, Length=218, Percent_Identity=29.8165137614679, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790281, Length=188, Percent_Identity=30.3191489361702, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=31.7073170731707, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=37.1428571428571, Blast_Score=149, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17554946, Length=243, Percent_Identity=35.3909465020576, Blast_Score=140, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=32.4675324675325, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17540306, Length=234, Percent_Identity=32.9059829059829, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=226, Percent_Identity=28.3185840707965, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=230, Percent_Identity=27.3913043478261, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=224, Percent_Identity=26.3392857142857, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=230, Percent_Identity=27.3913043478261, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=25.3968253968254, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=177, Percent_Identity=31.638418079096, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=186, Percent_Identity=27.9569892473118, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=186, Percent_Identity=27.9569892473118, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=194, Percent_Identity=29.8969072164948, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=194, Percent_Identity=29.8969072164948, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=194, Percent_Identity=29.8969072164948, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=26.2886597938144, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=35.9183673469388, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=35.9183673469388, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=35.632183908046, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19922422, Length=245, Percent_Identity=27.7551020408163, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=28.6821705426357, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24650670, Length=235, Percent_Identity=29.3617021276596, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357171, Length=244, Percent_Identity=27.8688524590164, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27291; Mature: 27160

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQSRIEGAVGWMTMDRPAAMNSLNREMAAAMTAQLKAWREDPQVRAVVLTGNGRAFCA
CCHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEE
GADLKEVAEPVVTGEPDFLDVVVGFVDTLRVFPKPVIAAVNGLALAGGLETVMACDLVLA
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AESAKLGDAHSDFGVFPGAGGAAVLPRKIPANVARWLLFTGATFTAAQMERYGLVNEVVA
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
DAELLARAQAVAELLATKSPLVLARMKRVVAEAADKSLADALRHELLELRNHQRSHDMKE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLTAFAEKRQPVFKGC
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLQSRIEGAVGWMTMDRPAAMNSLNREMAAAMTAQLKAWREDPQVRAVVLTGNGRAFCA
CHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEE
GADLKEVAEPVVTGEPDFLDVVVGFVDTLRVFPKPVIAAVNGLALAGGLETVMACDLVLA
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AESAKLGDAHSDFGVFPGAGGAAVLPRKIPANVARWLLFTGATFTAAQMERYGLVNEVVA
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
DAELLARAQAVAELLATKSPLVLARMKRVVAEAADKSLADALRHELLELRNHQRSHDMKE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLTAFAEKRQPVFKGC
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA