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The map label for this gene is phaA [H]

Identifier: 73538388

GI number: 73538388

Start: 1239536

End: 1240717

Strand: Reverse

Name: phaA [H]

Synonym: Reut_B4561

Alternate gene names: 73538388

Gene position: 1240717-1239536 (Counterclockwise)

Preceding gene: 73538389

Following gene: 73538387

Centisome position: 45.51

GC content: 69.71

Gene sequence:

>1182_bases
ATGCAAAACCCTGTGAAGGAATCTATCGTCATCGTCGGCGCGAAGCGCACGCCGATTGGCGCGTTCAATGGCTCCCTGGC
CGCACACAGCGCGCCCGAACTCGGCGCCATCGCCATCCGCGCGGCCGTGGCGCAGGTCGGCGTCGATCCGGCGTCGGTCG
ATGAAGCGATCATGGGCCTGTGCCTGTTCGCGGGCGTCGGCCAGGCTCCGGCCCGGCAGGCTGTGCTTGGCGCCGGCCTG
CCGACCTCGACACCGGCCACGACCCTGAGCAAGATGTGCGGCTCGGGCATGAAGTCGGTGATGCTCGCGCATGATCTGCT
GCTCGCCGGCACGTGCAATGTGGCAGTGGCGGGCGGCATGGAATCGATGACCAATGCGCCGCACATCGTTCCGCGCGGCC
GTCACGGCTACCGGCTCGGCCATGGGCAGTTGCTCGATCACATGTACTGCGATGGCCTGGAGGACGCCTACGAAGGCCAC
CTGATGGGCCATTACGCGGACCTGGCCGCGGCGGAGCGCGGCATCGGCCGCGAGGCGCAGGACGCCTATGCCCGCGAAAG
CCTGCAGCGTGCGCAGGCCGCAGTGGCCGAAGGGCGCTTCAGCGACGAGATCGCGCCGGTCGCGCTGAAGCGCGGCGGCG
AGCTGGCACAGGACGAGACGCCGGGCCAGTGCGACGCCGAGAAGCTGCCTAAGCTGAAGGCAGTGTTCCGTTCCGGCGGC
ACGGTGACGGCCGGCAATGCGTCTTCGATCTCCGACGGCGCTGCGGCGCTGGTGCTGATGCGAGAAAGCGACGCTCGCCG
CCAGGGCCTGGCGCCGCTGGCCCGCATCGTTGCGCACGCTACGCACGCCAACCCGCCGCAGCAGTTCGCCGATGCGCCCG
TGCATGCGGCGCGCCGCGTGCTCGATAAGGCGGGCTGGACCGCGCGCGATGTGGACCTCTACGAGATCAACGAGGCCTTC
GCCGTGGTGACGATGATCGCGCTGGACGAACTTGGCATTGGCCATGACCGCCTCAACGTCAACGGCGGCGCCTGCGCGCT
CGGTCATCCCGTCGGCGCCACCGGTGCGCGCCTGCTGGTCACGCTGCTCCATGCGCTGAAGCAGCGCGGCGGCCAGCGCG
GTCTCGCGAGCCTGTGCCTTGGCGGCGGTGAGGCCGTGGCAGTCGGCGTGGAACTTTGCTGA

Upstream 100 bases:

>100_bases
CGCTGCTCGGCGACGCGCTGTTCGCCCAGGACAGCGCATCGAGCCAGGTGATCCTCGACTTCGCACGCAACCAGCTGGCC
GTTCCGGCCTGAGGCAAGAC

Downstream 100 bases:

>100_bases
GGGATATGCCATGAGCCTGCAATCCCGTATCGAAGGCGCCGTCGGCTGGATGACCATGGACCGCCCGGCGGCCATGAACA
GCCTGAACCGCGAGATGGCT

Product: thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MQNPVKESIVIVGAKRTPIGAFNGSLAAHSAPELGAIAIRAAVAQVGVDPASVDEAIMGLCLFAGVGQAPARQAVLGAGL
PTSTPATTLSKMCGSGMKSVMLAHDLLLAGTCNVAVAGGMESMTNAPHIVPRGRHGYRLGHGQLLDHMYCDGLEDAYEGH
LMGHYADLAAAERGIGREAQDAYARESLQRAQAAVAEGRFSDEIAPVALKRGGELAQDETPGQCDAEKLPKLKAVFRSGG
TVTAGNASSISDGAAALVLMRESDARRQGLAPLARIVAHATHANPPQQFADAPVHAARRVLDKAGWTARDVDLYEINEAF
AVVTMIALDELGIGHDRLNVNGGACALGHPVGATGARLLVTLLHALKQRGGQRGLASLCLGGGEAVAVGVELC

Sequences:

>Translated_393_residues
MQNPVKESIVIVGAKRTPIGAFNGSLAAHSAPELGAIAIRAAVAQVGVDPASVDEAIMGLCLFAGVGQAPARQAVLGAGL
PTSTPATTLSKMCGSGMKSVMLAHDLLLAGTCNVAVAGGMESMTNAPHIVPRGRHGYRLGHGQLLDHMYCDGLEDAYEGH
LMGHYADLAAAERGIGREAQDAYARESLQRAQAAVAEGRFSDEIAPVALKRGGELAQDETPGQCDAEKLPKLKAVFRSGG
TVTAGNASSISDGAAALVLMRESDARRQGLAPLARIVAHATHANPPQQFADAPVHAARRVLDKAGWTARDVDLYEINEAF
AVVTMIALDELGIGHDRLNVNGGACALGHPVGATGARLLVTLLHALKQRGGQRGLASLCLGGGEAVAVGVELC
>Mature_393_residues
MQNPVKESIVIVGAKRTPIGAFNGSLAAHSAPELGAIAIRAAVAQVGVDPASVDEAIMGLCLFAGVGQAPARQAVLGAGL
PTSTPATTLSKMCGSGMKSVMLAHDLLLAGTCNVAVAGGMESMTNAPHIVPRGRHGYRLGHGQLLDHMYCDGLEDAYEGH
LMGHYADLAAAERGIGREAQDAYARESLQRAQAAVAEGRFSDEIAPVALKRGGELAQDETPGQCDAEKLPKLKAVFRSGG
TVTAGNASSISDGAAALVLMRESDARRQGLAPLARIVAHATHANPPQQFADAPVHAARRVLDKAGWTARDVDLYEINEAF
AVVTMIALDELGIGHDRLNVNGGACALGHPVGATGARLLVTLLHALKQRGGQRGLASLCLGGGEAVAVGVELC

Specific function: Involved in the production of polyhydroxyalkonic acids (PHAs), composed primarily of 3-hydroxybutyric acid (3HB) and 3- hydroxyvaleric acid (3HV) [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=396, Percent_Identity=47.4747474747475, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI148539872, Length=395, Percent_Identity=44.8101265822785, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI167614485, Length=388, Percent_Identity=38.1443298969072, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=34.6835443037975, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4504327, Length=435, Percent_Identity=30.5747126436782, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI194353979, Length=386, Percent_Identity=27.2020725388601, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI87082165, Length=389, Percent_Identity=47.3007712082262, Blast_Score=340, Evalue=7e-95,
Organism=Escherichia coli, GI1788554, Length=390, Percent_Identity=49.2307692307692, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=37.9052369077307, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI48994986, Length=402, Percent_Identity=35.0746268656716, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1788683, Length=413, Percent_Identity=30.7506053268765, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25147385, Length=376, Percent_Identity=42.0212765957447, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17535921, Length=387, Percent_Identity=42.3772609819121, Blast_Score=273, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI133906874, Length=387, Percent_Identity=39.2764857881137, Blast_Score=250, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI17535917, Length=382, Percent_Identity=35.0785340314136, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=32.0665083135392, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=394, Percent_Identity=44.4162436548223, Blast_Score=306, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6322031, Length=388, Percent_Identity=34.5360824742268, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24655093, Length=387, Percent_Identity=46.2532299741602, Blast_Score=330, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24640423, Length=388, Percent_Identity=45.360824742268, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI17648125, Length=389, Percent_Identity=36.7609254498715, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=29.6912114014252, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40421; Mature: 40421

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPVKESIVIVGAKRTPIGAFNGSLAAHSAPELGAIAIRAAVAQVGVDPASVDEAIMGL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
CLFAGVGQAPARQAVLGAGLPTSTPATTLSKMCGSGMKSVMLAHDLLLAGTCNVAVAGGM
HHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCH
ESMTNAPHIVPRGRHGYRLGHGQLLDHMYCDGLEDAYEGHLMGHYADLAAAERGIGREAQ
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCHHH
DAYARESLQRAQAAVAEGRFSDEIAPVALKRGGELAQDETPGQCDAEKLPKLKAVFRSGG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
TVTAGNASSISDGAAALVLMRESDARRQGLAPLARIVAHATHANPPQQFADAPVHAARRV
CEECCCCCCCCCCCEEEEEEECCCHHHHCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHH
LDKAGWTARDVDLYEINEAFAVVTMIALDELGIGHDRLNVNGGACALGHPVGATGARLLV
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCHHHHHHH
TLLHALKQRGGQRGLASLCLGGGEAVAVGVELC
HHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MQNPVKESIVIVGAKRTPIGAFNGSLAAHSAPELGAIAIRAAVAQVGVDPASVDEAIMGL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
CLFAGVGQAPARQAVLGAGLPTSTPATTLSKMCGSGMKSVMLAHDLLLAGTCNVAVAGGM
HHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCH
ESMTNAPHIVPRGRHGYRLGHGQLLDHMYCDGLEDAYEGHLMGHYADLAAAERGIGREAQ
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCHHH
DAYARESLQRAQAAVAEGRFSDEIAPVALKRGGELAQDETPGQCDAEKLPKLKAVFRSGG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
TVTAGNASSISDGAAALVLMRESDARRQGLAPLARIVAHATHANPPQQFADAPVHAARRV
CEECCCCCCCCCCCEEEEEEECCCHHHHCHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHH
LDKAGWTARDVDLYEINEAFAVVTMIALDELGIGHDRLNVNGGACALGHPVGATGARLLV
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCHHHHHHH
TLLHALKQRGGQRGLASLCLGGGEAVAVGVELC
HHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782; 10427049 [H]