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The map label for this gene is pepQ [H]

Identifier: 73538325

GI number: 73538325

Start: 1170084

End: 1171274

Strand: Direct

Name: pepQ [H]

Synonym: Reut_B4498

Alternate gene names: 73538325

Gene position: 1170084-1171274 (Clockwise)

Preceding gene: 73538324

Following gene: 73538328

Centisome position: 42.92

GC content: 65.49

Gene sequence:

>1191_bases
ATGGCACACCTTAGCAAGGCAGTACACGACTATCGCCTGTCGGCCATGCACCGGCTGATGGACCGCCTGAGCGTCGATGC
GCTGGTCTTCGGCACGCCCGACTTCTTCCAGTACGCGACTAATTACCAGCTCGATGTCTGGCCGTGGGAGCGGCCTGTAT
TCATTATCGTGCCGCGCAACGGCGAAACCTTCGCGGTCATGAACGAGCTGTCGACGAACCACCTGCGCTTCGCCACCAGC
AAGGACACTGTGTGGATGACCGACGTCACGTTCTACGCCGAGCACCCGCGCGTGTCGAATCGGCTGCCGTTGCCCGCGCA
ACTGCCCGAGGTCGTCGCCGCGCTGCTCAGCGCCAAGGGGCTGGGCATGTCGCGCATCGCGGTCGACACGCAGCACCCGA
TTCTGGTCGGCGCGCAGCGCTACCTGCCCGAACTGACCTTGCGCCCGTCGCTTGCGGAAATGCGTAGCCTGCGCTGGGTC
AAGCATGCCGAGGAGCATCAGGTCATGCGCGACCTCGCGTCGCTGACCGACTGGGTGCAGGACCGCTACCGCGAAAACAT
CCGCCCCGGCCGGCTCGTGCAGGAACTCGATCACGCGATGGCCGCGCTGATGGTGGAAGAAGCAGCGAACCGCTTCCCCG
GCGAGGACTTCGACATCCTGAAATGCTGGACGCTGGCGGGCGCCGCGTCAGCCTCGCCGCACGGCGACGGTGCCGCCTGC
GGGTCGCGCATTGCGCGCGGCGATACCATCGTCAACCTGATCCTGCCGCGCCTCAATGGCCTCCATGTCGAGAACGAACG
TACATGGTTCTGCGGCCGGCCCGACAGCGAGCAGTCGCGGCTTTACCAGGTCGCGGCACAAGCGACCGACGCTGCGGTGG
CCGCTGCAGTTGTTGGCAAGCGCGTGTGCGACATGGACGCCGCGGCGCAGGCCGTGATCGAAGCCGCCGGCTGCGGGCAG
TATGTCTTCCACCGGACCGGCCACGCGGTCGGGCTGATGCTGCACGAGTATCCCGAGGACATGGCCTTCAATACCCGGCC
GCTGCTGGCGGGCGAAGTCTACTCATCGGAACCGGGGCTGTATGTCTACGGGCTAGGTGGATTCCGGCTCGATGACACCG
TCATCGTCGGAGAGAGGCCGGAAGTGATCGTCAAGACGCCGAAGACTCTCGAATACGCCACCGTGGCATAG

Upstream 100 bases:

>100_bases
GGCCGCCGACCGAAGTCGAGCGCTACATGAAGCGCGATGCCGACGCCTGGGGCCCGATCATCCGGCGCCTGAACATTTCC
CTCTGAGTATCGAGATTGAT

Downstream 100 bases:

>100_bases
GCACGTTGTGGCCGCCCCCTGCATGGCGGAAGCGCTATGCCAGCGTCAGTCCGCCATGCGCCAGCACTTGCCGAAACACT
TCCAGCCGCGCATCGCCCGT

Product: peptidase M24

Products: NA

Alternate protein names: X-Pro dipeptidase; Imidodipeptidase; Proline dipeptidase; Prolidase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAHLSKAVHDYRLSAMHRLMDRLSVDALVFGTPDFFQYATNYQLDVWPWERPVFIIVPRNGETFAVMNELSTNHLRFATS
KDTVWMTDVTFYAEHPRVSNRLPLPAQLPEVVAALLSAKGLGMSRIAVDTQHPILVGAQRYLPELTLRPSLAEMRSLRWV
KHAEEHQVMRDLASLTDWVQDRYRENIRPGRLVQELDHAMAALMVEEAANRFPGEDFDILKCWTLAGAASASPHGDGAAC
GSRIARGDTIVNLILPRLNGLHVENERTWFCGRPDSEQSRLYQVAAQATDAAVAAAVVGKRVCDMDAAAQAVIEAAGCGQ
YVFHRTGHAVGLMLHEYPEDMAFNTRPLLAGEVYSSEPGLYVYGLGGFRLDDTVIVGERPEVIVKTPKTLEYATVA

Sequences:

>Translated_396_residues
MAHLSKAVHDYRLSAMHRLMDRLSVDALVFGTPDFFQYATNYQLDVWPWERPVFIIVPRNGETFAVMNELSTNHLRFATS
KDTVWMTDVTFYAEHPRVSNRLPLPAQLPEVVAALLSAKGLGMSRIAVDTQHPILVGAQRYLPELTLRPSLAEMRSLRWV
KHAEEHQVMRDLASLTDWVQDRYRENIRPGRLVQELDHAMAALMVEEAANRFPGEDFDILKCWTLAGAASASPHGDGAAC
GSRIARGDTIVNLILPRLNGLHVENERTWFCGRPDSEQSRLYQVAAQATDAAVAAAVVGKRVCDMDAAAQAVIEAAGCGQ
YVFHRTGHAVGLMLHEYPEDMAFNTRPLLAGEVYSSEPGLYVYGLGGFRLDDTVIVGERPEVIVKTPKTLEYATVA
>Mature_395_residues
AHLSKAVHDYRLSAMHRLMDRLSVDALVFGTPDFFQYATNYQLDVWPWERPVFIIVPRNGETFAVMNELSTNHLRFATSK
DTVWMTDVTFYAEHPRVSNRLPLPAQLPEVVAALLSAKGLGMSRIAVDTQHPILVGAQRYLPELTLRPSLAEMRSLRWVK
HAEEHQVMRDLASLTDWVQDRYRENIRPGRLVQELDHAMAALMVEEAANRFPGEDFDILKCWTLAGAASASPHGDGAACG
SRIARGDTIVNLILPRLNGLHVENERTWFCGRPDSEQSRLYQVAAQATDAAVAAAVVGKRVCDMDAAAQAVIEAAGCGQY
VFHRTGHAVGLMLHEYPEDMAFNTRPLLAGEVYSSEPGLYVYGLGGFRLDDTVIVGERPEVIVKTPKTLEYATVA

Specific function: Splits dipeptides with a prolyl in the C-terminal position and a nonpolar amino acid at the N-terminal position [H]

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24B family. Archaeal-type prolidase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788728, Length=259, Percent_Identity=31.2741312741313, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR001131 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: =3.4.13.9 [H]

Molecular weight: Translated: 44026; Mature: 43895

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00491 PROLINE_PEPTIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHLSKAVHDYRLSAMHRLMDRLSVDALVFGTPDFFQYATNYQLDVWPWERPVFIIVPRN
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCEEEEECCCCCEEEEEECC
GETFAVMNELSTNHLRFATSKDTVWMTDVTFYAEHPRVSNRLPLPAQLPEVVAALLSAKG
CCEEEEEECCCCCCEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
LGMSRIAVDTQHPILVGAQRYLPELTLRPSLAEMRSLRWVKHAEEHQVMRDLASLTDWVQ
CCCEEEEECCCCCEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRYRENIRPGRLVQELDHAMAALMVEEAANRFPGEDFDILKCWTLAGAASASPHGDGAAC
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHEECCCCCCCCCCCCCHHH
GSRIARGDTIVNLILPRLNGLHVENERTWFCGRPDSEQSRLYQVAAQATDAAVAAAVVGK
HHHHHCCCHHHHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVCDMDAAAQAVIEAAGCGQYVFHRTGHAVGLMLHEYPEDMAFNTRPLLAGEVYSSEPGL
HHHCHHHHHHHHHHHCCCCHHHHHHCCCCEEHHHHHCCHHCCCCCCCCEECCCCCCCCCE
YVYGLGGFRLDDTVIVGERPEVIVKTPKTLEYATVA
EEEECCCEEECCEEEECCCCCEEEECCCCCCEEECC
>Mature Secondary Structure 
AHLSKAVHDYRLSAMHRLMDRLSVDALVFGTPDFFQYATNYQLDVWPWERPVFIIVPRN
CHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCEEEEECCCCCEEEEEECC
GETFAVMNELSTNHLRFATSKDTVWMTDVTFYAEHPRVSNRLPLPAQLPEVVAALLSAKG
CCEEEEEECCCCCCEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
LGMSRIAVDTQHPILVGAQRYLPELTLRPSLAEMRSLRWVKHAEEHQVMRDLASLTDWVQ
CCCEEEEECCCCCEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRYRENIRPGRLVQELDHAMAALMVEEAANRFPGEDFDILKCWTLAGAASASPHGDGAAC
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHEECCCCCCCCCCCCCHHH
GSRIARGDTIVNLILPRLNGLHVENERTWFCGRPDSEQSRLYQVAAQATDAAVAAAVVGK
HHHHHCCCHHHHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVCDMDAAAQAVIEAAGCGQYVFHRTGHAVGLMLHEYPEDMAFNTRPLLAGEVYSSEPGL
HHHCHHHHHHHHHHHCCCCHHHHHHCCCCEEHHHHHCCHHCCCCCCCCEECCCCCCCCCE
YVYGLGGFRLDDTVIVGERPEVIVKTPKTLEYATVA
EEEECCCEEECCEEEECCCCCEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9733678; 11223522; 11210522 [H]