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The map label for this gene is ilvB [H]

Identifier: 73538317

GI number: 73538317

Start: 1161107

End: 1162972

Strand: Direct

Name: ilvB [H]

Synonym: Reut_B4490

Alternate gene names: 73538317

Gene position: 1161107-1162972 (Clockwise)

Preceding gene: 73538311

Following gene: 73538321

Centisome position: 42.59

GC content: 62.75

Gene sequence:

>1866_bases
GTGACCCGAAATCCAGAACTTGCCCCCTCACTTCCCCTGACGCCTGCGCCATCGAGCGAGCGCTTGTCGGGCGCCGATAT
CATCCTGCGCGTCCTTAGCGAACAGGGCGTCGATACCGTATTTGGCTATAGCGGAGGCGCCATCCTGCCGACGTATGACG
CCGTTTTCCGCTTCAATGCCATGCACGCGGAAGATCCCGCGCGCCAGATCAGGCTCGTTGTTCCCGCCAACGAGCAGGCT
GCCGGCTTCATGGCCGCCGGTTATGCGCGTGCCAGCGGGAAAGTCGGCGTGTTCATGGTGACATCCGGGCCCGGCGCGAC
GAATGCGGTCACGCCAATTGCCGACTGCAATGGCGATTCCGTCCCGGTGGTGCTGATATGTGGCCAGGTGCCGCGCGCAG
CCATCGGTAGTGATGCCTTTCAGGAAGCCCCCGTCTTCAACATCATGTCCGCCTGCGCCAAGCAGGTCTTCCTCGTGACG
GATCCTGAAAAGCTCGAGCACACCCTGCGGACGGCATTCGAGATTGCCCGCACGGGGCGCCCCGGTCCCGTGGTCGTCGA
TGTGCCGAAGGATGTCCAGAACTGGACCGGTACCTTTCACGGTTCCGGCACCTTGCAGTTCCGCGGCTACTCTGAACGTC
TTCGCAAGGTGGCGCACGGCGCTCGCCTGGACGAAAAGAAGCGCGCCCGCTTCTTCGATCTTCTCGGACAAAGCAGGCGG
CCACTGCTGTATGTCGGTGGTGGCGTCATAGCGGCCGGTGCCACGGCCGAATTGCGTCAGTTCAGCGAGCGCTATGGCAT
CCCCATTGTGACGACGCTCATGGGACTCGGTGCAATGCCCGCGGATCACGAACTGTATCTTGGCATGCTTGGCATGCACG
GTGCCGCGTGTGCGAACTATGCCGTTGAGGATTGCGACTTCCTGATTGCTGTCGGTGCCCGATTTGATGATCGGGTTGCC
GGTGGGCGACCTGACGCGTTTGCTCCCGGTGCGCGATACGTTGCGCATATCGACATCGATGAAGCGGAGATCAACAAGAT
CAAGCGGGCGCACTGGAGCCATGTCGGCGATGCCAGGCGCGCGCTGGCGTCGCTGGCGGAACATGGCCCGATCGAGTGGT
CGCATTCGGACTGGCTCGATCACGTCGCCGAACTGAAACGGATTTACCGCATGAATTACGACCGGACCAGTCCCGCGATC
CAGCCACAGTCTGTCGTGGAGGTGCTCGGCCGGATCACCGGAGGGCGCGCGATTGTCAGCACTGGGGTTGGCCAGCACCA
GATGTGGGCGGCACAGTACTTCCGGTTCAGCGAACCCAGAAGCTTCCTGACATCCGGCAGCATGGGGACCATGGGCTTCG
GACTGCCCGCGGCAATCGGCGCGCAACTGGCGCGGCCCGACGCGCTGGTCATCGATATCGACGGCGACGGCAGCATCCGC
ATGAACCTCGGGGAACTGGAGACCGCGACGACGTACGGGGTACCCGTCAAGGTTCTTCTGCTCAACAATCGCGGTGACGG
GATGATCCGGCAGTGGCAGCGACTGTACTTCGAGGGGCGCTTTTGTGTTAGCGACAAGGCACTCCACCGCAAGGATTTTG
TCCTCGCGGCGCAGGCGGATGGCTTCGAGTTTGCGTGCCGCGTGGAAGACGCCGACAAGGTCGAGGCGGCGCTCAGGGCC
TTCGTCGAGTTCAATGGCCCTGCCCTGCTGGAGGTGATGGTTGACGAGAACGCTGACGTATTCCCGATGGTGGGACCCGG
CCAGAGTTACGCAAGCATGATCACCGGACCATTCATCCCTTCACGATCCGGGCCGGAAGGGGCGGAACATGGGGCGCCGC
ATCTCCCTGCCGCCGATATGTTCTAG

Upstream 100 bases:

>100_bases
AGCTGGTGCCGAACATAAGCTGTTCTCGAGTTGGAAGGGAAATTGTCCGTTAAATCCTGGCTGCTGATGTTGCCAGCACC
AATTTCCATGCGATCCGGCC

Downstream 100 bases:

>100_bases
CCGCCTGCGCGGACCATGGGAAGTGGAACAGAACGGAACCCGCAGGAACTTGCCACCTGCCCGTCCGTCAGCCACTCCCC
AGAAAGCACTGGCCGTGGAC

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTRNPELAPSLPLTPAPSSERLSGADIILRVLSEQGVDTVFGYSGGAILPTYDAVFRFNAMHAEDPARQIRLVVPANEQA
AGFMAAGYARASGKVGVFMVTSGPGATNAVTPIADCNGDSVPVVLICGQVPRAAIGSDAFQEAPVFNIMSACAKQVFLVT
DPEKLEHTLRTAFEIARTGRPGPVVVDVPKDVQNWTGTFHGSGTLQFRGYSERLRKVAHGARLDEKKRARFFDLLGQSRR
PLLYVGGGVIAAGATAELRQFSERYGIPIVTTLMGLGAMPADHELYLGMLGMHGAACANYAVEDCDFLIAVGARFDDRVA
GGRPDAFAPGARYVAHIDIDEAEINKIKRAHWSHVGDARRALASLAEHGPIEWSHSDWLDHVAELKRIYRMNYDRTSPAI
QPQSVVEVLGRITGGRAIVSTGVGQHQMWAAQYFRFSEPRSFLTSGSMGTMGFGLPAAIGAQLARPDALVIDIDGDGSIR
MNLGELETATTYGVPVKVLLLNNRGDGMIRQWQRLYFEGRFCVSDKALHRKDFVLAAQADGFEFACRVEDADKVEAALRA
FVEFNGPALLEVMVDENADVFPMVGPGQSYASMITGPFIPSRSGPEGAEHGAPHLPAADMF

Sequences:

>Translated_621_residues
MTRNPELAPSLPLTPAPSSERLSGADIILRVLSEQGVDTVFGYSGGAILPTYDAVFRFNAMHAEDPARQIRLVVPANEQA
AGFMAAGYARASGKVGVFMVTSGPGATNAVTPIADCNGDSVPVVLICGQVPRAAIGSDAFQEAPVFNIMSACAKQVFLVT
DPEKLEHTLRTAFEIARTGRPGPVVVDVPKDVQNWTGTFHGSGTLQFRGYSERLRKVAHGARLDEKKRARFFDLLGQSRR
PLLYVGGGVIAAGATAELRQFSERYGIPIVTTLMGLGAMPADHELYLGMLGMHGAACANYAVEDCDFLIAVGARFDDRVA
GGRPDAFAPGARYVAHIDIDEAEINKIKRAHWSHVGDARRALASLAEHGPIEWSHSDWLDHVAELKRIYRMNYDRTSPAI
QPQSVVEVLGRITGGRAIVSTGVGQHQMWAAQYFRFSEPRSFLTSGSMGTMGFGLPAAIGAQLARPDALVIDIDGDGSIR
MNLGELETATTYGVPVKVLLLNNRGDGMIRQWQRLYFEGRFCVSDKALHRKDFVLAAQADGFEFACRVEDADKVEAALRA
FVEFNGPALLEVMVDENADVFPMVGPGQSYASMITGPFIPSRSGPEGAEHGAPHLPAADMF
>Mature_620_residues
TRNPELAPSLPLTPAPSSERLSGADIILRVLSEQGVDTVFGYSGGAILPTYDAVFRFNAMHAEDPARQIRLVVPANEQAA
GFMAAGYARASGKVGVFMVTSGPGATNAVTPIADCNGDSVPVVLICGQVPRAAIGSDAFQEAPVFNIMSACAKQVFLVTD
PEKLEHTLRTAFEIARTGRPGPVVVDVPKDVQNWTGTFHGSGTLQFRGYSERLRKVAHGARLDEKKRARFFDLLGQSRRP
LLYVGGGVIAAGATAELRQFSERYGIPIVTTLMGLGAMPADHELYLGMLGMHGAACANYAVEDCDFLIAVGARFDDRVAG
GRPDAFAPGARYVAHIDIDEAEINKIKRAHWSHVGDARRALASLAEHGPIEWSHSDWLDHVAELKRIYRMNYDRTSPAIQ
PQSVVEVLGRITGGRAIVSTGVGQHQMWAAQYFRFSEPRSFLTSGSMGTMGFGLPAAIGAQLARPDALVIDIDGDGSIRM
NLGELETATTYGVPVKVLLLNNRGDGMIRQWQRLYFEGRFCVSDKALHRKDFVLAAQADGFEFACRVEDADKVEAALRAF
VEFNGPALLEVMVDENADVFPMVGPGQSYASMITGPFIPSRSGPEGAEHGAPHLPAADMF

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=598, Percent_Identity=24.247491638796, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI21361361, Length=523, Percent_Identity=24.8565965583174, Blast_Score=116, Evalue=6e-26,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=42.0869565217391, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1790104, Length=581, Percent_Identity=39.7590361445783, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1786717, Length=581, Percent_Identity=30.2925989672978, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=28.0210157618214, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788716, Length=575, Percent_Identity=26.0869565217391, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=549, Percent_Identity=25.3187613843352, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17531299, Length=595, Percent_Identity=25.0420168067227, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=595, Percent_Identity=25.0420168067227, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=590, Percent_Identity=43.5593220338983, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=22.2222222222222, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922626, Length=505, Percent_Identity=24.3564356435644, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 66946; Mature: 66815

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNPELAPSLPLTPAPSSERLSGADIILRVLSEQGVDTVFGYSGGAILPTYDAVFRFNA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHEECC
MHAEDPARQIRLVVPANEQAAGFMAAGYARASGKVGVFMVTSGPGATNAVTPIADCNGDS
CCCCCCCCEEEEEECCCCCCCCHHHHCEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
VPVVLICGQVPRAAIGSDAFQEAPVFNIMSACAKQVFLVTDPEKLEHTLRTAFEIARTGR
CEEEEEECCCCCHHHCCCHHHHCCHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHCCC
PGPVVVDVPKDVQNWTGTFHGSGTLQFRGYSERLRKVAHGARLDEKKRARFFDLLGQSRR
CCCEEEECCCHHHHCCCEECCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
PLLYVGGGVIAAGATAELRQFSERYGIPIVTTLMGLGAMPADHELYLGMLGMHGAACANY
CEEEECCCEEECCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCC
AVEDCDFLIAVGARFDDRVAGGRPDAFAPGARYVAHIDIDEAEINKIKRAHWSHVGDARR
HHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
ALASLAEHGPIEWSHSDWLDHVAELKRIYRMNYDRTSPAIQPQSVVEVLGRITGGRAIVS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEE
TGVGQHQMWAAQYFRFSEPRSFLTSGSMGTMGFGLPAAIGAQLARPDALVIDIDGDGSIR
CCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEE
MNLGELETATTYGVPVKVLLLNNRGDGMIRQWQRLYFEGRFCVSDKALHRKDFVLAAQAD
EECCCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHCCCEEECCCHHCCCCEEEEEECC
GFEFACRVEDADKVEAALRAFVEFNGPALLEVMVDENADVFPMVGPGQSYASMITGPFIP
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCHHHHHHCCCCCC
SRSGPEGAEHGAPHLPAADMF
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRNPELAPSLPLTPAPSSERLSGADIILRVLSEQGVDTVFGYSGGAILPTYDAVFRFNA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHEECC
MHAEDPARQIRLVVPANEQAAGFMAAGYARASGKVGVFMVTSGPGATNAVTPIADCNGDS
CCCCCCCCEEEEEECCCCCCCCHHHHCEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
VPVVLICGQVPRAAIGSDAFQEAPVFNIMSACAKQVFLVTDPEKLEHTLRTAFEIARTGR
CEEEEEECCCCCHHHCCCHHHHCCHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHCCC
PGPVVVDVPKDVQNWTGTFHGSGTLQFRGYSERLRKVAHGARLDEKKRARFFDLLGQSRR
CCCEEEECCCHHHHCCCEECCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
PLLYVGGGVIAAGATAELRQFSERYGIPIVTTLMGLGAMPADHELYLGMLGMHGAACANY
CEEEECCCEEECCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCC
AVEDCDFLIAVGARFDDRVAGGRPDAFAPGARYVAHIDIDEAEINKIKRAHWSHVGDARR
HHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
ALASLAEHGPIEWSHSDWLDHVAELKRIYRMNYDRTSPAIQPQSVVEVLGRITGGRAIVS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEE
TGVGQHQMWAAQYFRFSEPRSFLTSGSMGTMGFGLPAAIGAQLARPDALVIDIDGDGSIR
CCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEE
MNLGELETATTYGVPVKVLLLNNRGDGMIRQWQRLYFEGRFCVSDKALHRKDFVLAAQAD
EECCCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHCCCEEECCCHHCCCCEEEEEECC
GFEFACRVEDADKVEAALRAFVEFNGPALLEVMVDENADVFPMVGPGQSYASMITGPFIP
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCHHHHHHCCCCCC
SRSGPEGAEHGAPHLPAADMF
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]