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The map label for this gene is Dml [H]

Identifier: 73538301

GI number: 73538301

Start: 1141400

End: 1142284

Strand: Direct

Name: Dml [H]

Synonym: Reut_B4474

Alternate gene names: 73538301

Gene position: 1141400-1142284 (Clockwise)

Preceding gene: 73538299

Following gene: 73538302

Centisome position: 41.87

GC content: 65.08

Gene sequence:

>885_bases
ATGACTTCATCCGCTTCCTCCCGCAGACAGGCATTCCGGCAGCAGGCCGCGTCAAAGAATGCCCTGCTCATCCCCGGCGC
CTTCAACGCCATGAGTGCGCGGGTCATCGAAGACCTCGGCTTCAAGGCCGTGTACCTCACCGGCGCCGGTGTCACCAATA
TGTCATTCGGCCTGCCTGACCTCGGTTTCATCGGGCTCAGCGATATCGCCGAGCACACGGCGCGGGTCCGCGATGCGGTG
GAACTGCCGTTGCTCGTCGATGCGGATACCGGTTTCGGCAACGCGCTGAACGTGCGCCAGGCCGTACGCACCCTGGAGCG
CGCGGGCGCCGATGCGATCCAGCTCGAGGACCAGGTCATGCCGAAGAAATGCGGGCACTTTGCCGGCAAGGATGTCGTGA
GCACGGACGAGATGGTGGCCAAGATCCGGGCCGCGACGGACGCGCGCGACGACCCCGACTTCCAGATCGTCGCGCGCACG
GATGCCGCGGCCGTGCACGGCATCGAGGATGCGATCGATCGCGGACACCGCTTTGCCGAAGCCGGCGCCGATATCCTGTT
CTTCGAGGCGATCGAGGGTCAAAGCGAGATCGCGCGCCTGCCGGGGCTGTTCGATGTGCCGCTGCTGATCAATATCGTGA
TCGGCGGAAAAACGCCGGTGCAAGGGCTCGACGCGCTGGAGCGGCTTGGCTACGGCCTGGTGCTCTACGCGAACGCCGCA
TTGCAAGGCGCCGTGCGCGGCATGCAGCAGGCACTCGGCAACCTGCAGGCAAACGGCCAGATGGACGAGGATCCCGCCAT
CGTCGTGCCATTCAGCGAGCGCCAGCGGCTCGTCAGGAAGCCGATGTACGACGAACTCGATGCTCGCTACAAGACGGATG
AGTGA

Upstream 100 bases:

>100_bases
GTCGGGCACATCCGCATGATCGCGCCTCACCGATTCGACCTGTCCTGATTGCGCGGCTGTCAGGCTGCCGACGACACGCC
GCCTCACCGGAGAACCCCAC

Downstream 100 bases:

>100_bases
GTAGGGGACATCATCCGCCTGCGAAGCGCCCGAATGTGCGCCTCCGTCTGACAGCCGGTGCAGAGGCGATCATTTACATG
GCCCGGTAAGAATGTGCGGC

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTSSASSRRQAFRQQAASKNALLIPGAFNAMSARVIEDLGFKAVYLTGAGVTNMSFGLPDLGFIGLSDIAEHTARVRDAV
ELPLLVDADTGFGNALNVRQAVRTLERAGADAIQLEDQVMPKKCGHFAGKDVVSTDEMVAKIRAATDARDDPDFQIVART
DAAAVHGIEDAIDRGHRFAEAGADILFFEAIEGQSEIARLPGLFDVPLLINIVIGGKTPVQGLDALERLGYGLVLYANAA
LQGAVRGMQQALGNLQANGQMDEDPAIVVPFSERQRLVRKPMYDELDARYKTDE

Sequences:

>Translated_294_residues
MTSSASSRRQAFRQQAASKNALLIPGAFNAMSARVIEDLGFKAVYLTGAGVTNMSFGLPDLGFIGLSDIAEHTARVRDAV
ELPLLVDADTGFGNALNVRQAVRTLERAGADAIQLEDQVMPKKCGHFAGKDVVSTDEMVAKIRAATDARDDPDFQIVART
DAAAVHGIEDAIDRGHRFAEAGADILFFEAIEGQSEIARLPGLFDVPLLINIVIGGKTPVQGLDALERLGYGLVLYANAA
LQGAVRGMQQALGNLQANGQMDEDPAIVVPFSERQRLVRKPMYDELDARYKTDE
>Mature_293_residues
TSSASSRRQAFRQQAASKNALLIPGAFNAMSARVIEDLGFKAVYLTGAGVTNMSFGLPDLGFIGLSDIAEHTARVRDAVE
LPLLVDADTGFGNALNVRQAVRTLERAGADAIQLEDQVMPKKCGHFAGKDVVSTDEMVAKIRAATDARDDPDFQIVARTD
AAAVHGIEDAIDRGHRFAEAGADILFFEAIEGQSEIARLPGLFDVPLLINIVIGGKTPVQGLDALERLGYGLVLYANAAL
QGAVRGMQQALGNLQANGQMDEDPAIVVPFSERQRLVRKPMYDELDARYKTDE

Specific function: Catalyzes the formation of proponate and pyruvate from (2R,3S)-2,3-dimethylmalate. Has no activity toward dimethylmaleate, malate, citramalate, isocitrate and citrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=257, Percent_Identity=42.4124513618677, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1790445, Length=150, Percent_Identity=35.3333333333333, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.32 [H]

Molecular weight: Translated: 31612; Mature: 31481

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSASSRRQAFRQQAASKNALLIPGAFNAMSARVIEDLGFKAVYLTGAGVTNMSFGLPD
CCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
LGFIGLSDIAEHTARVRDAVELPLLVDADTGFGNALNVRQAVRTLERAGADAIQLEDQVM
CCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHH
PKKCGHFAGKDVVSTDEMVAKIRAATDARDDPDFQIVARTDAAAVHGIEDAIDRGHRFAE
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
AGADILFFEAIEGQSEIARLPGLFDVPLLINIVIGGKTPVQGLDALERLGYGLVLYANAA
CCCCEEEEEECCCHHHHHHCCCCCCCHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEHHH
LQGAVRGMQQALGNLQANGQMDEDPAIVVPFSERQRLVRKPMYDELDARYKTDE
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHCCCHHHHCCCCCCCC
>Mature Secondary Structure 
TSSASSRRQAFRQQAASKNALLIPGAFNAMSARVIEDLGFKAVYLTGAGVTNMSFGLPD
CCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
LGFIGLSDIAEHTARVRDAVELPLLVDADTGFGNALNVRQAVRTLERAGADAIQLEDQVM
CCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHH
PKKCGHFAGKDVVSTDEMVAKIRAATDARDDPDFQIVARTDAAAVHGIEDAIDRGHRFAE
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
AGADILFFEAIEGQSEIARLPGLFDVPLLINIVIGGKTPVQGLDALERLGYGLVLYANAA
CCCCEEEEEECCCHHHHHHCCCCCCCHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEHHH
LQGAVRGMQQALGNLQANGQMDEDPAIVVPFSERQRLVRKPMYDELDARYKTDE
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA