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The map label for this gene is paaF [C]

Identifier: 73538288

GI number: 73538288

Start: 1127065

End: 1127910

Strand: Reverse

Name: paaF [C]

Synonym: Reut_B4461

Alternate gene names: 73538288

Gene position: 1127910-1127065 (Counterclockwise)

Preceding gene: 73538289

Following gene: 73538287

Centisome position: 41.37

GC content: 69.74

Gene sequence:

>846_bases
ATGCCCGACACCTCGCAGAACGACGGCGTAATCGTTCGCCGCTCCGGCGGCGTGCTGTTCGCCACGCTGAATATTCCCGC
CACGCGCAATGCGCTGGCACCTGAAGTCGTGGCGGCGCTGGCCGCGGCGGTGGAACAGGCGGAGACCGATCCCGAAGTCC
GCGCGCTGGTGTTGCGCGGCGCGGGCGGCATCTTCAGCGCCGGCGGCAACGTCGGTAATTTCCAGGCGCGCCTGCAGGCC
GACGCTAGCCAGGAAGACCCGGTCGCCACGCGCAACCGCCAGTTCGGCTATTTCATGGAGCGCCTGTCGGTGCTGCCGGT
GCCGGTGATTGCTGCCGTGGATGGTGCTGCGATGGGCGGAGGCATGGGCCTGGCGTGCGCGGCGGACATTGTGCTGGCCA
CGCGCGATGCGCGCTTTGCGCTGTCGGAAACCACGCTGGGCATCATTGCCGCGCAGATCGCGCCGTTCGTTGTGCAGCGC
CTTGGTGCGGTGGCGACGCGCCGTCTCGGGCTGACCGGTGAGCGCGTCAGCGGCCAGTCGACGGTGGCCATCGGGCTCGT
CGATCAGCTTGCCGAAGACAGCGCCGAGCTTGACGCGCTGATCGCCGAATGGCTGACGCGCGTCGGCCGCTGCGGGCCGA
ACGCGAACCGCGTGTTCAAGACGCTGGTGGGCCGCTGCGGTCGCGAGCCTGTATCGCCGCTGCTCGACGAGGCCTCGCAC
CTGTTTGCTCAATGCATGCGGCAAGAAGGCGCCGAGGGCGTTGCTGCCTTCCGCGAGAAGCGCGACGCCAGCTGGGTCGC
GCGCTTCGATGCCGACACCGTCCGCGCGGCGCAGGCCGTCGCCTGA

Upstream 100 bases:

>100_bases
CGGCGCGGACGAGGTGATGCTGCAGGTCATCGCCAAGCAGATGGGCCTGATGTCGCGCGGCTGAGCGCGTGTCCTTCCAC
ATTTGACGAGACACACTGCC

Downstream 100 bases:

>100_bases
GATTTCAAAGACTCAAAGAGATTCACGGGATTCCCTCATGTCCGCTACCCGCCGACCCTTCCATACGCTGCTCGTCGCCA
ACCGCGGCGAGATTGCGGTG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MPDTSQNDGVIVRRSGGVLFATLNIPATRNALAPEVVAALAAAVEQAETDPEVRALVLRGAGGIFSAGGNVGNFQARLQA
DASQEDPVATRNRQFGYFMERLSVLPVPVIAAVDGAAMGGGMGLACAADIVLATRDARFALSETTLGIIAAQIAPFVVQR
LGAVATRRLGLTGERVSGQSTVAIGLVDQLAEDSAELDALIAEWLTRVGRCGPNANRVFKTLVGRCGREPVSPLLDEASH
LFAQCMRQEGAEGVAAFREKRDASWVARFDADTVRAAQAVA

Sequences:

>Translated_281_residues
MPDTSQNDGVIVRRSGGVLFATLNIPATRNALAPEVVAALAAAVEQAETDPEVRALVLRGAGGIFSAGGNVGNFQARLQA
DASQEDPVATRNRQFGYFMERLSVLPVPVIAAVDGAAMGGGMGLACAADIVLATRDARFALSETTLGIIAAQIAPFVVQR
LGAVATRRLGLTGERVSGQSTVAIGLVDQLAEDSAELDALIAEWLTRVGRCGPNANRVFKTLVGRCGREPVSPLLDEASH
LFAQCMRQEGAEGVAAFREKRDASWVARFDADTVRAAQAVA
>Mature_280_residues
PDTSQNDGVIVRRSGGVLFATLNIPATRNALAPEVVAALAAAVEQAETDPEVRALVLRGAGGIFSAGGNVGNFQARLQAD
ASQEDPVATRNRQFGYFMERLSVLPVPVIAAVDGAAMGGGMGLACAADIVLATRDARFALSETTLGIIAAQIAPFVVQRL
GAVATRRLGLTGERVSGQSTVAIGLVDQLAEDSAELDALIAEWLTRVGRCGPNANRVFKTLVGRCGREPVSPLLDEASHL
FAQCMRQEGAEGVAAFREKRDASWVARFDADTVRAAQAVA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=262, Percent_Identity=29.0076335877863, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI37594471, Length=197, Percent_Identity=28.4263959390863, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=197, Percent_Identity=28.4263959390863, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=29.3436293436293, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=29.8850574712644, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI221142681, Length=273, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=25.9842519685039, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=32.2033898305085, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787660, Length=190, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082183, Length=248, Percent_Identity=24.5967741935484, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=29.2553191489362, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=272, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=263, Percent_Identity=28.5171102661597, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=191, Percent_Identity=28.7958115183246, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=264, Percent_Identity=24.6212121212121, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=199, Percent_Identity=28.643216080402, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=199, Percent_Identity=28.643216080402, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922422, Length=266, Percent_Identity=25.5639097744361, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=27.5193798449612, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19920382, Length=191, Percent_Identity=30.3664921465969, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=26.3565891472868, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=26.3565891472868, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29369; Mature: 29238

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTSQNDGVIVRRSGGVLFATLNIPATRNALAPEVVAALAAAVEQAETDPEVRALVLRG
CCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEEEC
AGGIFSAGGNVGNFQARLQADASQEDPVATRNRQFGYFMERLSVLPVPVIAAVDGAAMGG
CCCEEECCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCC
GMGLACAADIVLATRDARFALSETTLGIIAAQIAPFVVQRLGAVATRRLGLTGERVSGQS
CCHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TVAIGLVDQLAEDSAELDALIAEWLTRVGRCGPNANRVFKTLVGRCGREPVSPLLDEASH
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
LFAQCMRQEGAEGVAAFREKRDASWVARFDADTVRAAQAVA
HHHHHHHHCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
PDTSQNDGVIVRRSGGVLFATLNIPATRNALAPEVVAALAAAVEQAETDPEVRALVLRG
CCCCCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEEEC
AGGIFSAGGNVGNFQARLQADASQEDPVATRNRQFGYFMERLSVLPVPVIAAVDGAAMGG
CCCEEECCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCC
GMGLACAADIVLATRDARFALSETTLGIIAAQIAPFVVQRLGAVATRRLGLTGERVSGQS
CCHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TVAIGLVDQLAEDSAELDALIAEWLTRVGRCGPNANRVFKTLVGRCGREPVSPLLDEASH
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
LFAQCMRQEGAEGVAAFREKRDASWVARFDADTVRAAQAVA
HHHHHHHHCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA