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The map label for this gene is benB [H]

Identifier: 73538233

GI number: 73538233

Start: 1062031

End: 1062522

Strand: Direct

Name: benB [H]

Synonym: Reut_B4404

Alternate gene names: 73538233

Gene position: 1062031-1062522 (Clockwise)

Preceding gene: 73538232

Following gene: 73538234

Centisome position: 38.96

GC content: 61.59

Gene sequence:

>492_bases
ATGAGCACCGTCGACATCGCCGCCGTCCAGGCGTTCCTGTACCGCGAAGCCCGCCTGCTTGACGACGAGCAGTGGGACGA
GTGGCTGACCTGCTACCACGCCGAGGCGCGCTTCTGGATGCCGTGCTGGGACGACGACGGCCAGCTCATCACCGATCCGG
AGCGCGAGATCTCGCTGATCTTCTACCCGAGCCGCCAGGGCCTGGAAGACCGCATCTTTCGCATCAAGACCGAGCGTTCG
AGCGCGACGATTCCGGACACGCGCACGTGCCACAACCTCAGCAACGTGGAGATCGTGGAACGCGACGGCGACAAGCTGAC
CGTACGCTTCAACTGGCACACGCTCGCGCACCGGTACCAGACCAACTACAGCTACTTCGGCATGTCGCGCTACGTGATCG
ACTTTGCCGGCGCGCACCCGCAGATCCTGGACAAGTATGTCGTGCTCAAGAACGACTACATCAACCAGGTTATCGACATC
TATCACATCTGA

Upstream 100 bases:

>100_bases
AGGATGAAGGGCTCTACACCGTGCAACACCGCTACTGGCTCGACGTCATGAAGAAGGCCATGGCCGAGCAAGACACGGAA
GACTGCAACGGGAGCGCCGC

Downstream 100 bases:

>100_bases
GTAGGGTTCGCCGGCCCGCGGAGATTCCGTTCGGGCCAGCGGACACGGCTGGAGGACAAGGCCATGGAACACACGATCGC
ACTGCAATTCGAGGACGGCG

Product: 2-chlorobenzoate 1,2-dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MSTVDIAAVQAFLYREARLLDDEQWDEWLTCYHAEARFWMPCWDDDGQLITDPEREISLIFYPSRQGLEDRIFRIKTERS
SATIPDTRTCHNLSNVEIVERDGDKLTVRFNWHTLAHRYQTNYSYFGMSRYVIDFAGAHPQILDKYVVLKNDYINQVIDI
YHI

Sequences:

>Translated_163_residues
MSTVDIAAVQAFLYREARLLDDEQWDEWLTCYHAEARFWMPCWDDDGQLITDPEREISLIFYPSRQGLEDRIFRIKTERS
SATIPDTRTCHNLSNVEIVERDGDKLTVRFNWHTLAHRYQTNYSYFGMSRYVIDFAGAHPQILDKYVVLKNDYINQVIDI
YHI
>Mature_162_residues
STVDIAAVQAFLYREARLLDDEQWDEWLTCYHAEARFWMPCWDDDGQLITDPEREISLIFYPSRQGLEDRIFRIKTERSS
ATIPDTRTCHNLSNVEIVERDGDKLTVRFNWHTLAHRYQTNYSYFGMSRYVIDFAGAHPQILDKYVVLKNDYINQVIDIY
HI

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB). The beta subunit may be responsible for the substrate specificity of the enzyme [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017641
- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 19418; Mature: 19287

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVDIAAVQAFLYREARLLDDEQWDEWLTCYHAEARFWMPCWDDDGQLITDPEREISLI
CCCHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCEEECCEECCCCCEEECCCCEEEEE
FYPSRQGLEDRIFRIKTERSSATIPDTRTCHNLSNVEIVERDGDKLTVRFNWHTLAHRYQ
EECCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCEEEEECCCCEEEEEEEHHEEEHHHC
TNYSYFGMSRYVIDFAGAHPQILDKYVVLKNDYINQVIDIYHI
CCCEEECCEEEEEEECCCCHHHHHEEEEEECHHHHHHHHHCCC
>Mature Secondary Structure 
STVDIAAVQAFLYREARLLDDEQWDEWLTCYHAEARFWMPCWDDDGQLITDPEREISLI
CCHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCEEECCEECCCCCEEECCCCEEEEE
FYPSRQGLEDRIFRIKTERSSATIPDTRTCHNLSNVEIVERDGDKLTVRFNWHTLAHRYQ
EECCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCEEEEECCCCEEEEEEEHHEEEHHHC
TNYSYFGMSRYVIDFAGAHPQILDKYVVLKNDYINQVIDIYHI
CCCEEECCEEEEEEECCCCHHHHHEEEEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]