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The map label for this gene is benA [H]

Identifier: 73538232

GI number: 73538232

Start: 1060637

End: 1062034

Strand: Direct

Name: benA [H]

Synonym: Reut_B4403

Alternate gene names: 73538232

Gene position: 1060637-1062034 (Clockwise)

Preceding gene: 73538231

Following gene: 73538233

Centisome position: 38.91

GC content: 63.73

Gene sequence:

>1398_bases
ATGATCCCCATCCACGTGGACCGGGGCACGGCCCCCGCTCGCCCGCGCCTCTCGCTCGACCAGTTCCTCGTCGAAAACCA
TGAGACCGGCGACTACCGCCTGCATCGCAGCGCGTTCACCGACGAAGCACTGTTCGAACTCGAGATGAAGCACATCTTCG
AGGGCAACTGGATCTACCTGGCCCACGAAAGCCAGATCCCGAACAACAACGACTACTACACCACGCACATGGGCCGCCAG
CCCGTGTTCATCGCCCGCAACCGCCAGGGCGAACTGAATGCCTTCATCAACGCATGCACCCACCGCGGCGCCATGCTGTG
CCGGCACAAGCGCGGCAACAAGGCCACCTACACCTGCCCGTTCCACGGCTGGACCTTCAACAACAGCGGCAAGCTGCTCA
AGGTGAAGGACCCCGAAGGCGCCGGCTACCCCGAGTGCTTCAACAAGGAAGGCTCGCACGACCTGAAGAAGGTGGCGCGC
TTCGAGAACTACCGCGGCTTCCTGTTCGGCAGCCTCAATGACGACGTGCTGCCGCTCAAGGATTTCCTCGGCGATGCGGC
ACGCATCATCGACATGATTGCCGACCAGTCGCCCGATGGCCTGGAAGTGCTGCGCGGCTCGTCCACCTACACCTACGAAG
GCAACTGGAAGCTGCAGGCCGAAAACGGCGCCGACGGCTACCACGTATCCGCCGTGCACTGGAACTACGCGGCCACCACC
AACCACCGCAAGCAGCAGAACGAGCGCGAAGACAAGATCCGCGCGATGGATGCCGGCAAGTGGGGCCAGCAAGGCGGCGG
CTTCTACGCGTTCGATCATGGCCATATGCTGCTGTGGACGCGCTGGGCCAACCCGGAAGACCGGCCCAACTTCGCGCGCC
GCGCTGAATTCGCCGAGCGCTGCGGAGAGGAAACCGCGGACTGGATGATCCAGAACTCGCGCAACCTGTGCCTGTACCCG
AACGTGTACCTGATGGACCAGTTCGGCTCGCAGATCCGCCTGCTGCGCCCGCTGTCGGTCGACAAGACCGAAGTCACGAT
CTACTGCATCGCGCCGAAGGGAGAACCCGACGACGCCCGTGCGCGCCGCATCCGCCAGTACGAAGATTTCTTCAACGTGA
GCGGCATGGCCACGCCGGACGATCTCGAGGAATTCCGCGCCTGTCAGCAGGGCTACAACGGCAGCGCGCTGGCCTGGAAC
GACATGTGCCGCGGCTCCAAGCACTGGATCGAAGGCCCCGATGAAGCGGCGCAACGCATCGGCCTGAACCCCGTCATGAG
CGGCGTGCGGACCGAGGATGAAGGGCTCTACACCGTGCAACACCGCTACTGGCTCGACGTCATGAAGAAGGCCATGGCCG
AGCAAGACACGGAAGACTGCAACGGGAGCGCCGCATGA

Upstream 100 bases:

>100_bases
CTGAGGCTTCCAGATGACGCTGCCGGCAGGCAGCACCCACCGGGGACGGCCGCCCGGTTCCACCAACGGGCCGAAAGACA
CCGAATCAGGAGACGATTCC

Downstream 100 bases:

>100_bases
GCACCGTCGACATCGCCGCCGTCCAGGCGTTCCTGTACCGCGAAGCCCGCCTGCTTGACGACGAGCAGTGGGACGAGTGG
CTGACCTGCTACCACGCCGA

Product: benzoate 1,2-dioxygenase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MIPIHVDRGTAPARPRLSLDQFLVENHETGDYRLHRSAFTDEALFELEMKHIFEGNWIYLAHESQIPNNNDYYTTHMGRQ
PVFIARNRQGELNAFINACTHRGAMLCRHKRGNKATYTCPFHGWTFNNSGKLLKVKDPEGAGYPECFNKEGSHDLKKVAR
FENYRGFLFGSLNDDVLPLKDFLGDAARIIDMIADQSPDGLEVLRGSSTYTYEGNWKLQAENGADGYHVSAVHWNYAATT
NHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWTRWANPEDRPNFARRAEFAERCGEETADWMIQNSRNLCLYP
NVYLMDQFGSQIRLLRPLSVDKTEVTIYCIAPKGEPDDARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYNGSALAWN
DMCRGSKHWIEGPDEAAQRIGLNPVMSGVRTEDEGLYTVQHRYWLDVMKKAMAEQDTEDCNGSAA

Sequences:

>Translated_465_residues
MIPIHVDRGTAPARPRLSLDQFLVENHETGDYRLHRSAFTDEALFELEMKHIFEGNWIYLAHESQIPNNNDYYTTHMGRQ
PVFIARNRQGELNAFINACTHRGAMLCRHKRGNKATYTCPFHGWTFNNSGKLLKVKDPEGAGYPECFNKEGSHDLKKVAR
FENYRGFLFGSLNDDVLPLKDFLGDAARIIDMIADQSPDGLEVLRGSSTYTYEGNWKLQAENGADGYHVSAVHWNYAATT
NHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWTRWANPEDRPNFARRAEFAERCGEETADWMIQNSRNLCLYP
NVYLMDQFGSQIRLLRPLSVDKTEVTIYCIAPKGEPDDARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYNGSALAWN
DMCRGSKHWIEGPDEAAQRIGLNPVMSGVRTEDEGLYTVQHRYWLDVMKKAMAEQDTEDCNGSAA
>Mature_465_residues
MIPIHVDRGTAPARPRLSLDQFLVENHETGDYRLHRSAFTDEALFELEMKHIFEGNWIYLAHESQIPNNNDYYTTHMGRQ
PVFIARNRQGELNAFINACTHRGAMLCRHKRGNKATYTCPFHGWTFNNSGKLLKVKDPEGAGYPECFNKEGSHDLKKVAR
FENYRGFLFGSLNDDVLPLKDFLGDAARIIDMIADQSPDGLEVLRGSSTYTYEGNWKLQAENGADGYHVSAVHWNYAATT
NHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWTRWANPEDRPNFARRAEFAERCGEETADWMIQNSRNLCLYP
NVYLMDQFGSQIRLLRPLSVDKTEVTIYCIAPKGEPDDARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYNGSALAWN
DMCRGSKHWIEGPDEAAQRIGLNPVMSGVRTEDEGLYTVQHRYWLDVMKKAMAEQDTEDCNGSAA

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=391, Percent_Identity=29.6675191815857, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788103, Length=198, Percent_Identity=28.7878787878788, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 53236; Mature: 53236

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIHVDRGTAPARPRLSLDQFLVENHETGDYRLHRSAFTDEALFELEMKHIFEGNWIYL
CCEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEHHHCCHHHHHHHHHHHHCCCCEEEE
AHESQIPNNNDYYTTHMGRQPVFIARNRQGELNAFINACTHRGAMLCRHKRGNKATYTCP
EECCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEC
FHGWTFNNSGKLLKVKDPEGAGYPECFNKEGSHDLKKVARFENYRGFLFGSLNDDVLPLK
CCCEEECCCCCEEEEECCCCCCCHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHH
DFLGDAARIIDMIADQSPDGLEVLRGSSTYTYEGNWKLQAENGADGYHVSAVHWNYAATT
HHHHHHHHHHHHHHCCCCCHHHHEECCCEEEECCCEEEEECCCCCCEEEEEEEEEEEECC
NHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWTRWANPEDRPNFARRAEFAER
CHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
CGEETADWMIQNSRNLCLYPNVYLMDQFGSQIRLLRPLSVDKTEVTIYCIAPKGEPDDAR
HCCHHHHHHHCCCCCEEECCCEEEECCCCCCEEEEECCCCCCEEEEEEEECCCCCCCHHH
ARRIRQYEDFFNVSGMATPDDLEEFRACQQGYNGSALAWNDMCRGSKHWIEGPDEAAQRI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCCCHHHHHHHC
GLNPVMSGVRTEDEGLYTVQHRYWLDVMKKAMAEQDTEDCNGSAA
CCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIPIHVDRGTAPARPRLSLDQFLVENHETGDYRLHRSAFTDEALFELEMKHIFEGNWIYL
CCEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEHHHCCHHHHHHHHHHHHCCCCEEEE
AHESQIPNNNDYYTTHMGRQPVFIARNRQGELNAFINACTHRGAMLCRHKRGNKATYTCP
EECCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEC
FHGWTFNNSGKLLKVKDPEGAGYPECFNKEGSHDLKKVARFENYRGFLFGSLNDDVLPLK
CCCEEECCCCCEEEEECCCCCCCHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHH
DFLGDAARIIDMIADQSPDGLEVLRGSSTYTYEGNWKLQAENGADGYHVSAVHWNYAATT
HHHHHHHHHHHHHHCCCCCHHHHEECCCEEEECCCEEEEECCCCCCEEEEEEEEEEEECC
NHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWTRWANPEDRPNFARRAEFAER
CHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
CGEETADWMIQNSRNLCLYPNVYLMDQFGSQIRLLRPLSVDKTEVTIYCIAPKGEPDDAR
HCCHHHHHHHCCCCCEEECCCEEEECCCCCCEEEEECCCCCCEEEEEEEECCCCCCCHHH
ARRIRQYEDFFNVSGMATPDDLEEFRACQQGYNGSALAWNDMCRGSKHWIEGPDEAAQRI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCCCHHHHHHHC
GLNPVMSGVRTEDEGLYTVQHRYWLDVMKKAMAEQDTEDCNGSAA
CCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]