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The map label for this gene is rfaF [H]

Identifier: 73538215

GI number: 73538215

Start: 1043163

End: 1044410

Strand: Reverse

Name: rfaF [H]

Synonym: Reut_B4386

Alternate gene names: 73538215

Gene position: 1044410-1043163 (Counterclockwise)

Preceding gene: 73538216

Following gene: 73538214

Centisome position: 38.31

GC content: 68.27

Gene sequence:

>1248_bases
ATGACGACATGGGATCGCGCACGCCGGATCCTGTGTGTCCGGCTCGACAATATGGGCGATGTCCTGATGACCACGCCCGC
GATGCAGGCCCTTGCCGAAGCAGCACCGGGGCGGCGCCTGACCTTGCTGACCTCGGCCGCATCGGCCCGGCTCGCTCCGC
ACCTGCCGATGATCGACGACGTGATCGGCTGGAATGCGCCGTGGGTACGCCATGCCGACACGCCCTCGGCAGCCGAAGCC
GCGCGCCAGTTGCGCGCGTGTGCGGCGCAACTGGCCAGCGACCGCTACGACGGCGCCGTGATCTTTACCGTGTACACCCA
GAGCGCGCTGCCAGCAGCCATGCTATGCACGCTCGCCCGGATTCCGCTGCGGCTCGCGCATTGCCGCGAAAACCCTTACG
CGCTGCTGAGCCACTGGGCCAAGGAGACCGATCCGGGCGGACAGCCGCGCCACGAGGCCCGGCGCCAGCTCGATCTTGTG
GCGCAGATTGGCGCGCACACGGCGGATTCGCGTCTGCGTTTTGCCGTGCACGATGCGGACCGCGCCGCATTGGCGGTACG
GCTCGCGGCGCTGCCGGCGCATGCGCCGGCAAAAGCGAGGATCGTGCTTCACCCGGGCGCAACGGCGCCATCGCGCCGCT
GGCCCGCCGAACACTTTGCCGCAGTCGCGGGGCAACTGGCGCGGGACACGGGCGCGCAGGTGTTCGTGACCGGATCGGAC
ACGGAACGCGATCTCGTGGACGGCGTATGCCGGGCGGCGGGACACCCCGATGTCGTGCCGCTCGCCGGCATGCTGCCGCT
GGGCGAATTCGGGGCACTGCTCGAAATCGCCGACCTGCTGGTATCGAACAACAGTGGGCCCGTGCATATCGCGGCGGCGC
TTGGCACACCGGTCGTCGACCTGTACGCGCTGACCAATCCGCAGCATACGCCGTGGCAGGTGCCGCACCGCACGCTGTTC
GAGGACGTGCCGTGCCGCTATTGCTACAAGAGCACGTGCCCGCAGGGCCATCACCGCTGCCTGCGCGGCGTCAGCCCGCC
GGTTGTGGTGGCTGCGGCGCAGGCGCTGCTCGTCGGCGAGCCGGCAACCGAATGCCGGATGCCATGGCTGCCGAAATCGG
AATCGGAATCGGAATCGGAATCGGAATCGGAATCGGAAACGGAACCCGAACGCGAACCAGCCGGCGAACCGGAAAGCAAG
CCGGTGTCGACCGTAGAGGAGAGCACGCATGTACACGCTTGGCATTAA

Upstream 100 bases:

>100_bases
GGCGCACGCCGGACTTCATCGTGCCGACGCTCGGACAGGCCGCGCAGATCGTGGCGGGCGAGCCTGTGGCCGCCTGCGCA
CCCTTCGTGGCGGGGCAAGC

Downstream 100 bases:

>100_bases
CGCCGCCTACCACGATTCCGCCGCCTGTCTCGTACGCGACGGCGAGGTGATTGCCGCCGCCGAAGACGAACGCTTTACGC
ACATCAAGCACGGCAAGCGG

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTTWDRARRILCVRLDNMGDVLMTTPAMQALAEAAPGRRLTLLTSAASARLAPHLPMIDDVIGWNAPWVRHADTPSAAEA
ARQLRACAAQLASDRYDGAVIFTVYTQSALPAAMLCTLARIPLRLAHCRENPYALLSHWAKETDPGGQPRHEARRQLDLV
AQIGAHTADSRLRFAVHDADRAALAVRLAALPAHAPAKARIVLHPGATAPSRRWPAEHFAAVAGQLARDTGAQVFVTGSD
TERDLVDGVCRAAGHPDVVPLAGMLPLGEFGALLEIADLLVSNNSGPVHIAAALGTPVVDLYALTNPQHTPWQVPHRTLF
EDVPCRYCYKSTCPQGHHRCLRGVSPPVVVAAAQALLVGEPATECRMPWLPKSESESESESESESETEPEREPAGEPESK
PVSTVEESTHVHAWH

Sequences:

>Translated_415_residues
MTTWDRARRILCVRLDNMGDVLMTTPAMQALAEAAPGRRLTLLTSAASARLAPHLPMIDDVIGWNAPWVRHADTPSAAEA
ARQLRACAAQLASDRYDGAVIFTVYTQSALPAAMLCTLARIPLRLAHCRENPYALLSHWAKETDPGGQPRHEARRQLDLV
AQIGAHTADSRLRFAVHDADRAALAVRLAALPAHAPAKARIVLHPGATAPSRRWPAEHFAAVAGQLARDTGAQVFVTGSD
TERDLVDGVCRAAGHPDVVPLAGMLPLGEFGALLEIADLLVSNNSGPVHIAAALGTPVVDLYALTNPQHTPWQVPHRTLF
EDVPCRYCYKSTCPQGHHRCLRGVSPPVVVAAAQALLVGEPATECRMPWLPKSESESESESESESETEPEREPAGEPESK
PVSTVEESTHVHAWH
>Mature_414_residues
TTWDRARRILCVRLDNMGDVLMTTPAMQALAEAAPGRRLTLLTSAASARLAPHLPMIDDVIGWNAPWVRHADTPSAAEAA
RQLRACAAQLASDRYDGAVIFTVYTQSALPAAMLCTLARIPLRLAHCRENPYALLSHWAKETDPGGQPRHEARRQLDLVA
QIGAHTADSRLRFAVHDADRAALAVRLAALPAHAPAKARIVLHPGATAPSRRWPAEHFAAVAGQLARDTGAQVFVTGSDT
ERDLVDGVCRAAGHPDVVPLAGMLPLGEFGALLEIADLLVSNNSGPVHIAAALGTPVVDLYALTNPQHTPWQVPHRTLFE
DVPCRYCYKSTCPQGHHRCLRGVSPPVVVAAAQALLVGEPATECRMPWLPKSESESESESESESETEPEREPAGEPESKP
VSTVEESTHVHAWH

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=367, Percent_Identity=24.5231607629428, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 44767; Mature: 44636

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTWDRARRILCVRLDNMGDVLMTTPAMQALAEAAPGRRLTLLTSAASARLAPHLPMIDD
CCCHHHHCEEEEEEECCCCCCEEECHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCHHHH
VIGWNAPWVRHADTPSAAEAARQLRACAAQLASDRYDGAVIFTVYTQSALPAAMLCTLAR
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHH
IPLRLAHCRENPYALLSHWAKETDPGGQPRHEARRQLDLVAQIGAHTADSRLRFAVHDAD
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
RAALAVRLAALPAHAPAKARIVLHPGATAPSRRWPAEHFAAVAGQLARDTGAQVFVTGSD
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
TERDLVDGVCRAAGHPDVVPLAGMLPLGEFGALLEIADLLVSNNSGPVHIAAALGTPVVD
CHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEE
LYALTNPQHTPWQVPHRTLFEDVPCRYCYKSTCPQGHHRCLRGVSPPVVVAAAQALLVGE
EEEECCCCCCCCCCCHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCC
PATECRMPWLPKSESESESESESESETEPEREPAGEPESKPVSTVEESTHVHAWH
CHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEECCC
>Mature Secondary Structure 
TTWDRARRILCVRLDNMGDVLMTTPAMQALAEAAPGRRLTLLTSAASARLAPHLPMIDD
CCHHHHCEEEEEEECCCCCCEEECHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCHHHH
VIGWNAPWVRHADTPSAAEAARQLRACAAQLASDRYDGAVIFTVYTQSALPAAMLCTLAR
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHH
IPLRLAHCRENPYALLSHWAKETDPGGQPRHEARRQLDLVAQIGAHTADSRLRFAVHDAD
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
RAALAVRLAALPAHAPAKARIVLHPGATAPSRRWPAEHFAAVAGQLARDTGAQVFVTGSD
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
TERDLVDGVCRAAGHPDVVPLAGMLPLGEFGALLEIADLLVSNNSGPVHIAAALGTPVVD
CHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEE
LYALTNPQHTPWQVPHRTLFEDVPCRYCYKSTCPQGHHRCLRGVSPPVVVAAAQALLVGE
EEEECCCCCCCCCCCHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCC
PATECRMPWLPKSESESESESESESETEPEREPAGEPESKPVSTVEESTHVHAWH
CHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]