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The map label for this gene is phnD [H]

Identifier: 73538008

GI number: 73538008

Start: 821797

End: 822777

Strand: Direct

Name: phnD [H]

Synonym: Reut_B4178

Alternate gene names: 73538008

Gene position: 821797-822777 (Clockwise)

Preceding gene: 73538007

Following gene: 73538009

Centisome position: 30.14

GC content: 61.57

Gene sequence:

>981_bases
ATGCTTCGCAGAACATTCCTCGCCGTTGTCGCATCGACCGTTGTTGCCATGCCGGCCTTTGCGCAGGACGCCAAGACGCT
GAACATCGGCTTCATCTCGACGGAGTCGTCGTCGAACCTGAGGTCGGCCTGGCAGCCGTTGATCGATGACCTCAGCAAGA
CGCTGGGCGCGACCGTCAAGCCGTTCTTTGCCTCGGACTATGCCGGCATCATCGAAGGCATGCGCTTCAACAAGGTCCAG
ATCGCCTGGTTCGGCAACAAGTCGGCCATGGAAGCCGTGGACCGCGCCAATGGCGAAGTGTTCGCCTCGGTGATCGACAA
GGACGGCAACCCGGGCTACTGGTCCCTGCTGATCGTCAACAAGGACAGCGATATCAAGAACGTCGAAGACGTGATCAAGC
GCGGCAAGGAACTCAGTTACGGCGCCGGCGATCCGAACTCGACGTCGGGCACGGCCGTGCCGGGCTACTACCTGTGGGCC
GCCCACAAGGTCGAGCCGAAGACGCTGTTCAAGGCCGTTCGCATCAGCAACCACGAAACCAACCTGCTTTCGGTGCTGAA
CAAGCAGGTGGACGTGGCCGTGAACAACACCGAGAACTTCGAGCGCTATCGCATCAACACGGGCAAGAATCCGTATGACC
AGGTGCGCGTGCTGTGGAAGTCGCCGCTGATCCCGGCCGACCCGATCGTTTATCGCAAGGACCTGCCGGCCGACGTGAAG
AAGAAGATCCAGACCTTCTTCGTCAACTACGGCAAGGGCCCTGACCCCGCGCGCGAGAAGCAGGTGCTGGCCACGCTGAC
GTACCAGGGCTTCCGTCCGTCGACCGATGCGCAACTCGTGCCGATCCGCCAGATCGAGCTGGCCAAGGAAAAGGCTCGCA
TCGAGACCGACACCACACTGGCCGCTGCCGACAAGCAGAAGCAGCTCGCCGACGTGAGCCGACGCCTGGCCGACCTGGAC
AAGGCTGTCAGCACACAGTAA

Upstream 100 bases:

>100_bases
GGCCTGACGGCTGTCTTGCATCGCGTTCTTTTCCAGATCCAAGGCGTACTCAAGACGTATTCAACACGTTCGCCAAACCG
AATTCCAACCGGAGTCATCC

Downstream 100 bases:

>100_bases
GTCCCACCCGCGGCTGGCGGCATGCCGCCAGCCGCGGCATTGCATTTTCTCTTCTCGCATTACCGGATTGTCCCCATGAC
CACCACTGCCAAGCACGCCC

Product: phosphonate-binding periplasmic protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLRRTFLAVVASTVVAMPAFAQDAKTLNIGFISTESSSNLRSAWQPLIDDLSKTLGATVKPFFASDYAGIIEGMRFNKVQ
IAWFGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIVNKDSDIKNVEDVIKRGKELSYGAGDPNSTSGTAVPGYYLWA
AHKVEPKTLFKAVRISNHETNLLSVLNKQVDVAVNNTENFERYRINTGKNPYDQVRVLWKSPLIPADPIVYRKDLPADVK
KKIQTFFVNYGKGPDPAREKQVLATLTYQGFRPSTDAQLVPIRQIELAKEKARIETDTTLAAADKQKQLADVSRRLADLD
KAVSTQ

Sequences:

>Translated_326_residues
MLRRTFLAVVASTVVAMPAFAQDAKTLNIGFISTESSSNLRSAWQPLIDDLSKTLGATVKPFFASDYAGIIEGMRFNKVQ
IAWFGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIVNKDSDIKNVEDVIKRGKELSYGAGDPNSTSGTAVPGYYLWA
AHKVEPKTLFKAVRISNHETNLLSVLNKQVDVAVNNTENFERYRINTGKNPYDQVRVLWKSPLIPADPIVYRKDLPADVK
KKIQTFFVNYGKGPDPAREKQVLATLTYQGFRPSTDAQLVPIRQIELAKEKARIETDTTLAAADKQKQLADVSRRLADLD
KAVSTQ
>Mature_326_residues
MLRRTFLAVVASTVVAMPAFAQDAKTLNIGFISTESSSNLRSAWQPLIDDLSKTLGATVKPFFASDYAGIIEGMRFNKVQ
IAWFGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIVNKDSDIKNVEDVIKRGKELSYGAGDPNSTSGTAVPGYYLWA
AHKVEPKTLFKAVRISNHETNLLSVLNKQVDVAVNNTENFERYRINTGKNPYDQVRVLWKSPLIPADPIVYRKDLPADVK
KKIQTFFVNYGKGPDPAREKQVLATLTYQGFRPSTDAQLVPIRQIELAKEKARIETDTTLAAADKQKQLADVSRRLADLD
KAVSTQ

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=317, Percent_Identity=51.1041009463722, Blast_Score=321, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36129; Mature: 36129

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRTFLAVVASTVVAMPAFAQDAKTLNIGFISTESSSNLRSAWQPLIDDLSKTLGATVK
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
PFFASDYAGIIEGMRFNKVQIAWFGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIVN
CHHHCCHHHHHCCCEECEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEE
KDSDIKNVEDVIKRGKELSYGAGDPNSTSGTAVPGYYLWAAHKVEPKTLFKAVRISNHET
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCH
NLLSVLNKQVDVAVNNTENFERYRINTGKNPYDQVRVLWKSPLIPADPIVYRKDLPADVK
HHHHHHHHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHH
KKIQTFFVNYGKGPDPAREKQVLATLTYQGFRPSTDAQLVPIRQIELAKEKARIETDTTL
HHHHHHHHCCCCCCCCHHHHHHHHEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCHH
AAADKQKQLADVSRRLADLDKAVSTQ
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRRTFLAVVASTVVAMPAFAQDAKTLNIGFISTESSSNLRSAWQPLIDDLSKTLGATVK
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
PFFASDYAGIIEGMRFNKVQIAWFGNKSAMEAVDRANGEVFASVIDKDGNPGYWSLLIVN
CHHHCCHHHHHCCCEECEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEE
KDSDIKNVEDVIKRGKELSYGAGDPNSTSGTAVPGYYLWAAHKVEPKTLFKAVRISNHET
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCH
NLLSVLNKQVDVAVNNTENFERYRINTGKNPYDQVRVLWKSPLIPADPIVYRKDLPADVK
HHHHHHHHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHH
KKIQTFFVNYGKGPDPAREKQVLATLTYQGFRPSTDAQLVPIRQIELAKEKARIETDTTL
HHHHHHHHCCCCCCCCHHHHHHHHEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCHH
AAADKQKQLADVSRRLADLDKAVSTQ
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]