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The map label for this gene is phnE [H]

Identifier: 73538009

GI number: 73538009

Start: 822799

End: 823665

Strand: Direct

Name: phnE [H]

Synonym: Reut_B4179

Alternate gene names: 73538009

Gene position: 822799-823665 (Clockwise)

Preceding gene: 73538008

Following gene: 73538010

Centisome position: 30.18

GC content: 64.24

Gene sequence:

>867_bases
ATGCCGCCAGCCGCGGCATTGCATTTTCTCTTCTCGCATTACCGGATTGTCCCCATGACCACCACTGCCAAGCACGCCCT
GCCGCCCTCTCCCAAGAGCCAGGTGCGGCCCCCGCGCACACCGCTTTCCGTGATGCTGATCTGGGCGATCGTGCTGGCGA
TGCTTGTCCTGTCCTGGAACGGCGCCGACATGCGTCCGCTGGACCTGCTGCGCGATTCCGGCAACATGGCGCAGTTCGCC
GCCGACTTCTTCCCCCCCGATTTCCGCGACTGGCGCCATTACCTGGACGAAATGCTCGTCACGGTGCAGATCGCCGTATG
GGGCACGGCCATTGCCGTGTTCATGGCCGTGCCGTTCGGCTTGCTGTGCTCGGCCAATATCGTGCCCGCCTGGATCTACC
AGCCGGCGCGCCGTGTCATGGATGCCTGCCGCGCCATCAATGAGATGGTCTTCGCCATGCTGTTCATCGTGGCCGTCGGG
CTCGGTCCCTTCGCCGGCGTGCTGGCGCTCTGGATCCATACCACCGGGGTGCTGGCCAAGCTGTTTGCCGAAGCCGTCGA
AGCGATCGACCCGCGCCCTGTCGAAGGCGTGCGCGCCACCGGCGCCGGCCCTCTTGAAGAAATCATCTATGGCGTGATTC
CGCAGGTGCTGCCGCTCTGGCTCTCGTTCGCGCTGTATCGCTTCGAATCCAACGTGCGTTCGGCGTCGGTCGTGGGCATC
GTCGGCGCGGGCGGCATCGGCACCGTGCTGTGGGAAATCATCCGCAGCTTCCAGTACGGCCAGACCTGCGCGGTGATGAT
CATTATCATCGTCTTCGTGACCGCCATTGACGTGATCTCGGCGCAGATCCGCAAGGTGCTGGTCTGA

Upstream 100 bases:

>100_bases
CGCTGCCGACAAGCAGAAGCAGCTCGCCGACGTGAGCCGACGCCTGGCCGACCTGGACAAGGCTGTCAGCACACAGTAAG
TCCCACCCGCGGCTGGCGGC

Downstream 100 bases:

>100_bases
TCGACACAAAAATTCCCCGCAAAATTTCCATGCTGACCCTGCCCCGCATTGCATCGCTGTTCGCCAGCCACGGTACTGCC
TACTACGGCGGCGAAGCCAT

Product: phosphonate ABC transporter permease

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MPPAAALHFLFSHYRIVPMTTTAKHALPPSPKSQVRPPRTPLSVMLIWAIVLAMLVLSWNGADMRPLDLLRDSGNMAQFA
ADFFPPDFRDWRHYLDEMLVTVQIAVWGTAIAVFMAVPFGLLCSANIVPAWIYQPARRVMDACRAINEMVFAMLFIVAVG
LGPFAGVLALWIHTTGVLAKLFAEAVEAIDPRPVEGVRATGAGPLEEIIYGVIPQVLPLWLSFALYRFESNVRSASVVGI
VGAGGIGTVLWEIIRSFQYGQTCAVMIIIIVFVTAIDVISAQIRKVLV

Sequences:

>Translated_288_residues
MPPAAALHFLFSHYRIVPMTTTAKHALPPSPKSQVRPPRTPLSVMLIWAIVLAMLVLSWNGADMRPLDLLRDSGNMAQFA
ADFFPPDFRDWRHYLDEMLVTVQIAVWGTAIAVFMAVPFGLLCSANIVPAWIYQPARRVMDACRAINEMVFAMLFIVAVG
LGPFAGVLALWIHTTGVLAKLFAEAVEAIDPRPVEGVRATGAGPLEEIIYGVIPQVLPLWLSFALYRFESNVRSASVVGI
VGAGGIGTVLWEIIRSFQYGQTCAVMIIIIVFVTAIDVISAQIRKVLV
>Mature_287_residues
PPAAALHFLFSHYRIVPMTTTAKHALPPSPKSQVRPPRTPLSVMLIWAIVLAMLVLSWNGADMRPLDLLRDSGNMAQFAA
DFFPPDFRDWRHYLDEMLVTVQIAVWGTAIAVFMAVPFGLLCSANIVPAWIYQPARRVMDACRAINEMVFAMLFIVAVGL
GPFAGVLALWIHTTGVLAKLFAEAVEAIDPRPVEGVRATGAGPLEEIIYGVIPQVLPLWLSFALYRFESNVRSASVVGIV
GAGGIGTVLWEIIRSFQYGQTCAVMIIIIVFVTAIDVISAQIRKVLV

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31571; Mature: 31440

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPAAALHFLFSHYRIVPMTTTAKHALPPSPKSQVRPPRTPLSVMLIWAIVLAMLVLSWN
CCHHHHHHHHHHCCEEEEECCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GADMRPLDLLRDSGNMAQFAADFFPPDFRDWRHYLDEMLVTVQIAVWGTAIAVFMAVPFG
CCCCCHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLCSANIVPAWIYQPARRVMDACRAINEMVFAMLFIVAVGLGPFAGVLALWIHTTGVLAK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LFAEAVEAIDPRPVEGVRATGAGPLEEIIYGVIPQVLPLWLSFALYRFESNVRSASVVGI
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEE
VGAGGIGTVLWEIIRSFQYGQTCAVMIIIIVFVTAIDVISAQIRKVLV
ECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PPAAALHFLFSHYRIVPMTTTAKHALPPSPKSQVRPPRTPLSVMLIWAIVLAMLVLSWN
CHHHHHHHHHHCCEEEEECCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GADMRPLDLLRDSGNMAQFAADFFPPDFRDWRHYLDEMLVTVQIAVWGTAIAVFMAVPFG
CCCCCHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLCSANIVPAWIYQPARRVMDACRAINEMVFAMLFIVAVGLGPFAGVLALWIHTTGVLAK
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LFAEAVEAIDPRPVEGVRATGAGPLEEIIYGVIPQVLPLWLSFALYRFESNVRSASVVGI
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEE
VGAGGIGTVLWEIIRSFQYGQTCAVMIIIIVFVTAIDVISAQIRKVLV
ECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]