Click here to switch to the map view.

The map label for this gene is amtB [C]

Identifier: 73537871

GI number: 73537871

Start: 680963

End: 682165

Strand: Reverse

Name: amtB [C]

Synonym: Reut_B4038

Alternate gene names: 73537871

Gene position: 682165-680963 (Counterclockwise)

Preceding gene: 73537875

Following gene: 73537870

Centisome position: 25.02

GC content: 62.34

Gene sequence:

>1203_bases
ATGGACAATTGGAAGACTGGCACCGACGCCATGTTCATCCTGCTTGGCGCAATCATGGTGCTGGCCATGCATGCGGGTTT
CGCCTTCCTGGAGCTAGGGACGGTACGCAAGAAAAACCAGGTCAATGCCCTGGTAAAGATTCTGGTGGACTTCGCGGTTT
CCACCATCGCGTACTTCTTCATCGGTTATACCGTCGCCTACGGCGTGCAATTCATGTCCGGTGCTGACACGCTGGCACAA
AAGAGCGGCTACGAACTGGTGAAGTTCTTTTTCCTGGCAACGTTTGCCGCCGCCATTCCGGCGATTATCTCGGGTGGCAT
TGCGGAGCGGTCCCGTTTCAACCCGCAATTGTGTGCCACCTTCGTGCTGGTCGGCTTTGTCTATCCGTTTTTCGAAGGGA
TTGCATGGAACCAGCGTTTTGGCATCCAGGACTGGATCACGCATCTGACTGGCGCGCCGTTCCATGACTTTGCCGGCTCC
GTGGTCGTCCATGCGGTGGGCGGCTGGATTGCTCTGCCCGCGGTACTGCTGCTCGGCGCTCGCCGGGGTCGCTACCAGAA
GGACGGCCGCATCAGCGCGCATCCGCCGTCGAACATCCCGTTCCTGGCATTGGGCGCCTGGGTGCTGGCGGTGGGTTGGT
TCGGCTTCAATGTCATGAGCGCCCAGACCGTCGACAAGATCAGCGGCCTGGTCGCCATCAATTCGCTGATGGCCATGGCT
GGCGGCACGCTGGCGGCCTGGTGGGCGGGTCGTAACGACCCGGGATTTGCCTACAACGGTCCGCTGGCAGGCCTGGTCGC
CGTCTGCGCCGGCTCGGACCTGATGCACCCGGTGGGCGCGCTGGTGACCGGCGCCGTTGCGGGCGTGCTGTTCGTGCATT
TGTTTACGCTGGCGCAGAACAAATGGCATATCGATGATGTCCTCGGCGTCTGGCCGCTGCACGGCCTGTGCGGCGCCTGG
GGCGGCATTGCGGCGGGCATCTTCGGCAGCCATGCGCTGGGTGGGATCGGCGGGGTTTCGCTTGGCGCGCAGCTGATCGG
TACCCTGCTCGGCATCGTGATTGCCGTGGCGGGTGGCACCATTGTCTACGGCACGCTCAAGAAGCTGGTCGGCATTCGCC
TGGATGCCGAAGCCGAATTCAATGGCGCCGACCTGACGCTGCATCGCATCTCGGCTACGCCCGAGCGCGAAACCAACTGG
TAA

Upstream 100 bases:

>100_bases
GGTGCGAACTCCACCATGAAGGTGCAACTGGCACCAGGATGCAGCCGCTTCTGGAACGCTTTTTGCATACCCATGCTCCC
ATTTTGAGCATGAGGGACCT

Downstream 100 bases:

>100_bases
GTCCCGGCGGAAACAGGCTCTCGTGGGCCTGTTCCTGACAATTCTTTGCTTGAACTTCCCCTGACGGCATGGCAATAATT
GCCGTGCCCGAAGTCTTGCG

Product: Rh-like protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDNWKTGTDAMFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTIAYFFIGYTVAYGVQFMSGADTLAQ
KSGYELVKFFFLATFAAAIPAIISGGIAERSRFNPQLCATFVLVGFVYPFFEGIAWNQRFGIQDWITHLTGAPFHDFAGS
VVVHAVGGWIALPAVLLLGARRGRYQKDGRISAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTVDKISGLVAINSLMAMA
GGTLAAWWAGRNDPGFAYNGPLAGLVAVCAGSDLMHPVGALVTGAVAGVLFVHLFTLAQNKWHIDDVLGVWPLHGLCGAW
GGIAAGIFGSHALGGIGGVSLGAQLIGTLLGIVIAVAGGTIVYGTLKKLVGIRLDAEAEFNGADLTLHRISATPERETNW

Sequences:

>Translated_400_residues
MDNWKTGTDAMFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTIAYFFIGYTVAYGVQFMSGADTLAQ
KSGYELVKFFFLATFAAAIPAIISGGIAERSRFNPQLCATFVLVGFVYPFFEGIAWNQRFGIQDWITHLTGAPFHDFAGS
VVVHAVGGWIALPAVLLLGARRGRYQKDGRISAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTVDKISGLVAINSLMAMA
GGTLAAWWAGRNDPGFAYNGPLAGLVAVCAGSDLMHPVGALVTGAVAGVLFVHLFTLAQNKWHIDDVLGVWPLHGLCGAW
GGIAAGIFGSHALGGIGGVSLGAQLIGTLLGIVIAVAGGTIVYGTLKKLVGIRLDAEAEFNGADLTLHRISATPERETNW
>Mature_400_residues
MDNWKTGTDAMFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTIAYFFIGYTVAYGVQFMSGADTLAQ
KSGYELVKFFFLATFAAAIPAIISGGIAERSRFNPQLCATFVLVGFVYPFFEGIAWNQRFGIQDWITHLTGAPFHDFAGS
VVVHAVGGWIALPAVLLLGARRGRYQKDGRISAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTVDKISGLVAINSLMAMA
GGTLAAWWAGRNDPGFAYNGPLAGLVAVCAGSDLMHPVGALVTGAVAGVLFVHLFTLAQNKWHIDDVLGVWPLHGLCGAW
GGIAAGIFGSHALGGIGGVSLGAQLIGTLLGIVIAVAGGTIVYGTLKKLVGIRLDAEAEFNGADLTLHRISATPERETNW

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=404, Percent_Identity=31.4356435643564, Blast_Score=141, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17550182, Length=434, Percent_Identity=29.9539170506912, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17550180, Length=426, Percent_Identity=28.6384976525822, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32564042, Length=352, Percent_Identity=28.125, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI133931100, Length=438, Percent_Identity=26.7123287671233, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321559, Length=360, Percent_Identity=25.5555555555556, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325396, Length=355, Percent_Identity=25.3521126760563, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324187, Length=403, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=29.8795180722892, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42238; Mature: 42238

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]