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The map label for this gene is sir1 [H]

Identifier: 73537774

GI number: 73537774

Start: 573063

End: 574769

Strand: Reverse

Name: sir1 [H]

Synonym: Reut_B3940

Alternate gene names: 73537774

Gene position: 574769-573063 (Counterclockwise)

Preceding gene: 73537775

Following gene: 73537773

Centisome position: 21.08

GC content: 64.21

Gene sequence:

>1707_bases
ATGTACCTCTATGACTCCACCGACCAGCAACTGGTCGACGAGCGCGTTACCCAGTTCACTGACCAGACCGCACGCTTCCT
GAGCGGTGATCTCACGGAAGACGAATTCCGTATCCTGCGTCTGCAGAACGGGCTCTACATCCAGCGTCATGCGCCGATGC
TGCGTGTGGCGATCCCCTACGGCATGCTGGTTTCGCGCCAACTGCGCAAGCTGGCCGAGATCTCGCGGCGCTGGGACCGC
GGCTACGGCCACTTCAGCACGCGCCAGAACATGCAGTTCAACTGGCCGCGCCTGGAAGACGCGCCTGCCATCCTGGCCGA
ACTCGCCACGGTCCAGATGCATGCGATCCAGACCAGCGGCAACTGCATCCGCAATACGACCACGGACCACTTTGCCGGCA
TCGCACCCGATGAGGTGGTGAATCCGCTGGTGTGGTGCGAAATCATCCGGCAATGGTCGATGCTGCATCCGGAATTCGCG
TTCCTTCCGCGCAAGTTCAAGATCGCCGTCAGTGGCGCCACCACCGACCGTGCCGCGGTAGGCGTGCACGACATCGGGCT
GCAGGCGGTCGAGCGCGACGGCGAAACCGGCTTCCGCGTGTGGGTGGGCGGCGGCATGGGGCGCACGCCGATGGTCGGCA
AGGAAATCAGCGGATTCGTGCACTGGTCCGACTTGCTCACCTACCTCCAGGCGATCCTGCGGGTTTACAACCTGCATGGC
CGGCGCGACAACAAGTACAAGGCGCGCATCAAGATTCTCGTGAAGGACCTTACGCCGGAAGTCTTCGCTCAGCAGGTCCA
TGAACACTGGCAGCCTATCCGCGGCGGCCCCGATACCGTCGATGCGGATTTCGTGCGCGACATCGCCACGCGCTTCACCA
CGCCGGATTACGACGCTGCCGCCGCGCAGGACCACGACCCGAACCCCGCGCTGGCCGCCGCCAACCCGCGCTTTGCGCGC
TGGCTGCGCAGCAACGTGCATGCGCACCGCGTGCCCGGCTATGCCGCCGTCACGGTATCGCTGAAGGCCACCGGCACGCC
GCCCGGCGACATCACCGCCGACCAGATGGAAGCCGCGGCAGACCTCGCGGACCACTACGGCTTTGGCGAGCTGCGCGTCT
CGCACGAACAGAACCTGATCATCGCCGACGTGCGTCGCACGCGCCTGCATGAACTGTGGCAGCAGCTGCAGACGTGGAAC
CTCGCCACGCCCAATATCGGCCTGCTCACCAACATCATCGCCTGCCCGGGCGGGGATTTCTGCTCGCTGGCCAATGCGGT
ATCGATCCCGCTGGCCGAGGCGATTCAGCAACGCTTCGACGATCTCGACTACCTCTACGAGATCGGCGAACTCGACCTGA
ACATCTCGGGCTGCATCAACTCTTGCGGACACCACCACGTCGGCCATATCGGCATCCTCGGCGTCGACAAGGCCGGCGAG
GAGTGGTACCAGGTCACCCTTGGCGGACGCCAGGGCGGGCCATCCGGTGCCGCAATCGGCCGCATCATCGGGCCATCGTT
CGCGCGCGATGAGATTCCGGAGGTGGTCGAGGAACTGATCAAGCGCTACATCGCGATGCGCGACAGTGAAGCGGAGCGCT
TCATCGATGTGGTCGAGCGTGTCGGTATCGAACCGTTCAAGGTTGCGGTCTATCAGAATCCGGTGATTGCAAAGCGCCGT
CTGGAAGCGGAGGCTGCCCGTGTCTGA

Upstream 100 bases:

>100_bases
GCAACTGCTGCTGTCCGCATGCCGGGGCCATCGCTAGCCTTGAGTCTGTGCCACGCCCGCAAGGCGTGGCTGGCCCAAGA
GCGATGCGAGACCAGCCCCC

Downstream 100 bases:

>100_bases
AGCCATGGCTGTGCTGTAGCAAGATACAAAATCGGAGAATGACCGCAGCAGTGCTCGCCGACGCGCTGCCTCTGTCACCT
TTAGGCGGGCCTGCTCCCTT

Product: nitrite/sulfite reductase, hemoprotein beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MYLYDSTDQQLVDERVTQFTDQTARFLSGDLTEDEFRILRLQNGLYIQRHAPMLRVAIPYGMLVSRQLRKLAEISRRWDR
GYGHFSTRQNMQFNWPRLEDAPAILAELATVQMHAIQTSGNCIRNTTTDHFAGIAPDEVVNPLVWCEIIRQWSMLHPEFA
FLPRKFKIAVSGATTDRAAVGVHDIGLQAVERDGETGFRVWVGGGMGRTPMVGKEISGFVHWSDLLTYLQAILRVYNLHG
RRDNKYKARIKILVKDLTPEVFAQQVHEHWQPIRGGPDTVDADFVRDIATRFTTPDYDAAAAQDHDPNPALAAANPRFAR
WLRSNVHAHRVPGYAAVTVSLKATGTPPGDITADQMEAAADLADHYGFGELRVSHEQNLIIADVRRTRLHELWQQLQTWN
LATPNIGLLTNIIACPGGDFCSLANAVSIPLAEAIQQRFDDLDYLYEIGELDLNISGCINSCGHHHVGHIGILGVDKAGE
EWYQVTLGGRQGGPSGAAIGRIIGPSFARDEIPEVVEELIKRYIAMRDSEAERFIDVVERVGIEPFKVAVYQNPVIAKRR
LEAEAARV

Sequences:

>Translated_568_residues
MYLYDSTDQQLVDERVTQFTDQTARFLSGDLTEDEFRILRLQNGLYIQRHAPMLRVAIPYGMLVSRQLRKLAEISRRWDR
GYGHFSTRQNMQFNWPRLEDAPAILAELATVQMHAIQTSGNCIRNTTTDHFAGIAPDEVVNPLVWCEIIRQWSMLHPEFA
FLPRKFKIAVSGATTDRAAVGVHDIGLQAVERDGETGFRVWVGGGMGRTPMVGKEISGFVHWSDLLTYLQAILRVYNLHG
RRDNKYKARIKILVKDLTPEVFAQQVHEHWQPIRGGPDTVDADFVRDIATRFTTPDYDAAAAQDHDPNPALAAANPRFAR
WLRSNVHAHRVPGYAAVTVSLKATGTPPGDITADQMEAAADLADHYGFGELRVSHEQNLIIADVRRTRLHELWQQLQTWN
LATPNIGLLTNIIACPGGDFCSLANAVSIPLAEAIQQRFDDLDYLYEIGELDLNISGCINSCGHHHVGHIGILGVDKAGE
EWYQVTLGGRQGGPSGAAIGRIIGPSFARDEIPEVVEELIKRYIAMRDSEAERFIDVVERVGIEPFKVAVYQNPVIAKRR
LEAEAARV
>Mature_568_residues
MYLYDSTDQQLVDERVTQFTDQTARFLSGDLTEDEFRILRLQNGLYIQRHAPMLRVAIPYGMLVSRQLRKLAEISRRWDR
GYGHFSTRQNMQFNWPRLEDAPAILAELATVQMHAIQTSGNCIRNTTTDHFAGIAPDEVVNPLVWCEIIRQWSMLHPEFA
FLPRKFKIAVSGATTDRAAVGVHDIGLQAVERDGETGFRVWVGGGMGRTPMVGKEISGFVHWSDLLTYLQAILRVYNLHG
RRDNKYKARIKILVKDLTPEVFAQQVHEHWQPIRGGPDTVDADFVRDIATRFTTPDYDAAAAQDHDPNPALAAANPRFAR
WLRSNVHAHRVPGYAAVTVSLKATGTPPGDITADQMEAAADLADHYGFGELRVSHEQNLIIADVRRTRLHELWQQLQTWN
LATPNIGLLTNIIACPGGDFCSLANAVSIPLAEAIQQRFDDLDYLYEIGELDLNISGCINSCGHHHVGHIGILGVDKAGE
EWYQVTLGGRQGGPSGAAIGRIIGPSFARDEIPEVVEELIKRYIAMRDSEAERFIDVVERVGIEPFKVAVYQNPVIAKRR
LEAEAARV

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322597, Length=399, Percent_Identity=25.062656641604, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 63622; Mature: 63622

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLYDSTDQQLVDERVTQFTDQTARFLSGDLTEDEFRILRLQNGLYIQRHAPMLRVAIPY
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCEEEEECCH
GMLVSRQLRKLAEISRRWDRGYGHFSTRQNMQFNWPRLEDAPAILAELATVQMHAIQTSG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCC
NCIRNTTTDHFAGIAPDEVVNPLVWCEIIRQWSMLHPEFAFLPRKFKIAVSGATTDRAAV
CEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCCCCCCC
GVHDIGLQAVERDGETGFRVWVGGGMGRTPMVGKEISGFVHWSDLLTYLQAILRVYNLHG
CHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHHHHHHCC
RRDNKYKARIKILVKDLTPEVFAQQVHEHWQPIRGGPDTVDADFVRDIATRFTTPDYDAA
CCCCCCEEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
AAQDHDPNPALAAANPRFARWLRSNVHAHRVPGYAAVTVSLKATGTPPGDITADQMEAAA
CCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHH
DLADHYGFGELRVSHEQNLIIADVRRTRLHELWQQLQTWNLATPNIGLLTNIIACPGGDF
HHHHHCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCH
CSLANAVSIPLAEAIQQRFDDLDYLYEIGELDLNISGCINSCGHHHVGHIGILGVDKAGE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCCCEEEEEECCCCCC
EWYQVTLGGRQGGPSGAAIGRIIGPSFARDEIPEVVEELIKRYIAMRDSEAERFIDVVER
CEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VGIEPFKVAVYQNPVIAKRRLEAEAARV
CCCCCEEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MYLYDSTDQQLVDERVTQFTDQTARFLSGDLTEDEFRILRLQNGLYIQRHAPMLRVAIPY
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCEEEEECCH
GMLVSRQLRKLAEISRRWDRGYGHFSTRQNMQFNWPRLEDAPAILAELATVQMHAIQTSG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCC
NCIRNTTTDHFAGIAPDEVVNPLVWCEIIRQWSMLHPEFAFLPRKFKIAVSGATTDRAAV
CEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCCCCCCC
GVHDIGLQAVERDGETGFRVWVGGGMGRTPMVGKEISGFVHWSDLLTYLQAILRVYNLHG
CHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHEEHHHHHHHHHHHHHHHHHCC
RRDNKYKARIKILVKDLTPEVFAQQVHEHWQPIRGGPDTVDADFVRDIATRFTTPDYDAA
CCCCCCEEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
AAQDHDPNPALAAANPRFARWLRSNVHAHRVPGYAAVTVSLKATGTPPGDITADQMEAAA
CCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHH
DLADHYGFGELRVSHEQNLIIADVRRTRLHELWQQLQTWNLATPNIGLLTNIIACPGGDF
HHHHHCCCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCH
CSLANAVSIPLAEAIQQRFDDLDYLYEIGELDLNISGCINSCGHHHVGHIGILGVDKAGE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCCCEEEEEECCCCCC
EWYQVTLGGRQGGPSGAAIGRIIGPSFARDEIPEVVEELIKRYIAMRDSEAERFIDVVER
CEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VGIEPFKVAVYQNPVIAKRRLEAEAARV
CCCCCEEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA