Click here to switch to the map view.

The map label for this gene is ascD [H]

Identifier: 73537600

GI number: 73537600

Start: 361896

End: 362894

Strand: Direct

Name: ascD [H]

Synonym: Reut_B3765

Alternate gene names: 73537600

Gene position: 361896-362894 (Clockwise)

Preceding gene: 73537599

Following gene: 73537601

Centisome position: 13.27

GC content: 64.86

Gene sequence:

>999_bases
ATGTCGTACCGCATCCAGATCGCCGAGACGCAGCAGGTCTTTTTTGTCGAACGCGGCGAAACGCTGCTGCAGGCCGCCGG
GCGTGCGGATATCTCGCTGCCGCATGACTGCCAGCTCGGTGGCTGTGGCACTTGCCGCATACGTCTGCTCGACGGACAGG
TGCGGTATGACGAAGAACCGTTCGGGCTTGCGCCGGACGAGGCTGCTGCTGGCTATGCGCTGGCGTGCCAGGCACAGCCG
CTCGCCGACCTGGTCATCAGTACCGCGCGTGAAGACGAAGCGTGCGCCGAGCCGGCGCGGCATCAGGCCATCGTGCGCGG
CGTGCGCCCGTTGTCTTTGGACGTACTGCATGTCGAGCTTGAGGTGCCCGATGCCGGTACGCTTGCGTATCGCCCGGGCC
AGTACCTGAAGCTCGTGACGGGTGACGGCCTCGCGCGCAGCTTTTCGATGGCGTCGGTGCCAAGCGAAGGGCGGGTTGAC
CTGCATGTGCGGCGCATCCCGGGTGGCGCTTTTACCGACGGCATCCTGCCGAGGCTGAAGGCCGGTGACGCTGTCGATGT
CGAATTGCCGCTTGGCACCTTTTTCTATCGTGCGAAAGACTATCGGCCATTGTTGATGGTGGCCACGGGCACGGGGCTGG
CGCCGATCAAGGCGATCCTCGAGTCGCTGATGGACGATCCCGACTGTCCGCCCGTGTCGCTCTACTGGGGCATGCGCCAA
CCCGAAGACCTTTACCTGCACGCGGACATCCCGGCATGGGGAGAGCGGCTTTATGACTTCCAGTACGTGCCGGTGCTATC
GCGCGCCGGCGATGGCTGGCAGGGTCGGCGCGGCTACGTGCACGACGCGGCATTGGCCGACCTGGGCGATCTGAGCGAGC
ATGCGATCTACCTTTGCGGATCTCCGAACATGATCCGTGATGCCCGCGCCGCCTTCATCGCGCGGGGCGCCAGTCCCGAG
CATCTCTACGCGGACAGTTTTACCTTCCAGCGCGATTGA

Upstream 100 bases:

>100_bases
GCGCTGCAGGCGCTGGCGGAACTGCTGGCGCCGCAGGACCTGAAGGTCCTGGTGCAGGCCGATATCAAGAAGTAACGCAG
TCATCTTGCGCGGAGGTTCC

Downstream 100 bases:

>100_bases
TAAGACATTGCGGTATACAGTCGGAGGCTTGGTATACAATGAGGGCCAGTTCGTGTCCTGCTTCGCTTCCGTTCATCCAT
GGCCCTCACTTCGCCCACTG

Product: ferredoxin:oxidoreductase FAD/NAD(P)-binding:oxidoreductase FAD-binding region

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSYRIQIAETQQVFFVERGETLLQAAGRADISLPHDCQLGGCGTCRIRLLDGQVRYDEEPFGLAPDEAAAGYALACQAQP
LADLVISTAREDEACAEPARHQAIVRGVRPLSLDVLHVELEVPDAGTLAYRPGQYLKLVTGDGLARSFSMASVPSEGRVD
LHVRRIPGGAFTDGILPRLKAGDAVDVELPLGTFFYRAKDYRPLLMVATGTGLAPIKAILESLMDDPDCPPVSLYWGMRQ
PEDLYLHADIPAWGERLYDFQYVPVLSRAGDGWQGRRGYVHDAALADLGDLSEHAIYLCGSPNMIRDARAAFIARGASPE
HLYADSFTFQRD

Sequences:

>Translated_332_residues
MSYRIQIAETQQVFFVERGETLLQAAGRADISLPHDCQLGGCGTCRIRLLDGQVRYDEEPFGLAPDEAAAGYALACQAQP
LADLVISTAREDEACAEPARHQAIVRGVRPLSLDVLHVELEVPDAGTLAYRPGQYLKLVTGDGLARSFSMASVPSEGRVD
LHVRRIPGGAFTDGILPRLKAGDAVDVELPLGTFFYRAKDYRPLLMVATGTGLAPIKAILESLMDDPDCPPVSLYWGMRQ
PEDLYLHADIPAWGERLYDFQYVPVLSRAGDGWQGRRGYVHDAALADLGDLSEHAIYLCGSPNMIRDARAAFIARGASPE
HLYADSFTFQRD
>Mature_331_residues
SYRIQIAETQQVFFVERGETLLQAAGRADISLPHDCQLGGCGTCRIRLLDGQVRYDEEPFGLAPDEAAAGYALACQAQPL
ADLVISTAREDEACAEPARHQAIVRGVRPLSLDVLHVELEVPDAGTLAYRPGQYLKLVTGDGLARSFSMASVPSEGRVDL
HVRRIPGGAFTDGILPRLKAGDAVDVELPLGTFFYRAKDYRPLLMVATGTGLAPIKAILESLMDDPDCPPVSLYWGMRQP
EDLYLHADIPAWGERLYDFQYVPVLSRAGDGWQGRRGYVHDAALADLGDLSEHAIYLCGSPNMIRDARAAFIARGASPEH
LYADSFTFQRD

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=227, Percent_Identity=33.0396475770925, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788903, Length=268, Percent_Identity=25.3731343283582, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322146, Length=195, Percent_Identity=27.1794871794872, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 36319; Mature: 36187

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYRIQIAETQQVFFVERGETLLQAAGRADISLPHDCQLGGCGTCRIRLLDGQVRYDEEP
CCEEEEEECCEEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCC
FGLAPDEAAAGYALACQAQPLADLVISTAREDEACAEPARHQAIVRGVRPLSLDVLHVEL
CCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCCEEEEEEEEE
EVPDAGTLAYRPGQYLKLVTGDGLARSFSMASVPSEGRVDLHVRRIPGGAFTDGILPRLK
ECCCCCCEEECCCCEEEEEECCCCHHEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
AGDAVDVELPLGTFFYRAKDYRPLLMVATGTGLAPIKAILESLMDDPDCPPVSLYWGMRQ
CCCEEEEEECCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCC
PEDLYLHADIPAWGERLYDFQYVPVLSRAGDGWQGRRGYVHDAALADLGDLSEHAIYLCG
CCCEEEEECCCHHHHHHHCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEC
SPNMIRDARAAFIARGASPEHLYADSFTFQRD
CCCHHHHHHHHHHCCCCCCCEEEECCEEEECC
>Mature Secondary Structure 
SYRIQIAETQQVFFVERGETLLQAAGRADISLPHDCQLGGCGTCRIRLLDGQVRYDEEP
CEEEEEECCEEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCC
FGLAPDEAAAGYALACQAQPLADLVISTAREDEACAEPARHQAIVRGVRPLSLDVLHVEL
CCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCCEEEEEEEEE
EVPDAGTLAYRPGQYLKLVTGDGLARSFSMASVPSEGRVDLHVRRIPGGAFTDGILPRLK
ECCCCCCEEECCCCEEEEEECCCCHHEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
AGDAVDVELPLGTFFYRAKDYRPLLMVATGTGLAPIKAILESLMDDPDCPPVSLYWGMRQ
CCCEEEEEECCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCC
PEDLYLHADIPAWGERLYDFQYVPVLSRAGDGWQGRRGYVHDAALADLGDLSEHAIYLCG
CCCEEEEECCCHHHHHHHCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEC
SPNMIRDARAAFIARGASPEHLYADSFTFQRD
CCCHHHHHHHHHHCCCCCCCEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]