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The map label for this gene is paaK [H]

Identifier: 73537576

GI number: 73537576

Start: 334348

End: 335694

Strand: Direct

Name: paaK [H]

Synonym: Reut_B3741

Alternate gene names: 73537576

Gene position: 334348-335694 (Clockwise)

Preceding gene: 73537575

Following gene: 73537577

Centisome position: 12.26

GC content: 64.74

Gene sequence:

>1347_bases
ATGGTTCAACGCACCTCCCACCCCGACGAGCTGGAGCCGATCGAACGCGCCAGCCGCGATGAACTGCAGGCGCTGCAGCT
GGAACGGCTTAAATGGAGCGTGCGCCATGCCTATGACAACGTGCCGCATTACCGCAAGTCGTTTGACGCGGCGGGCGTGC
ATCCGGATGACCTGAAGTCGCTGTCGGACCTGTCGAAGTTCCCGTTCCTGGCCAAGCAGGACCTGCGCGACAACTACCCG
TTCGGCATGTTCGCCGTGCCGCGCGAGAAGGTGGCGCGCGTGCACGCATCGAGCGGCACCACGGGCAAGCCGACCGTGGT
GGGCTACACCGCACGCGATATCGACACGTGGGCGAGCGTGGTGGCGCGCTCGATTCGCGCCGCAGGGGGGCGTCCAGGCG
ATCTCGTGCACGTGAGCTACGGTTATGGCCTGTTCACGGGTGGCCTCGGCGCGCACTACGGTGCGGAAAAGGCGGGTTGC
ACGGTCATCCCGATGTCGGGCGGCCAGACCGAGAAACAGGTACAGCTGATCCGCGAGTTCCAGCCGAACATCATCATGGT
GACGCCGTCGTACATGCTGAACCTGATCGAGGAGATGATGCGCCAGGGTATGGATCCGGCCGAAAGCTCGCTGAAGGTTG
GCATCTTCGGCGCCGAGCCGTGGACCGATGCAATGCGTGCCGAGATCGAAGCGCGCGCGGGCATCGATGCGGTGGACATC
TACGGCCTGTCGGAAGTCATGGGACCGGGCGTGGCGTGCGAATGCATCGAAAGCAAGGACGGCCCGGTAATCTGGGAAGA
CCACTTCTATGCCGAGATCATCGATCCGGTCACCGGCGAAGTGCTGCCGGACGGCGAGGAGGGCGAACTGGTGTTCACCT
CGCTGACCAAGGAAGCGCTGCCGGTGATCCGCTACCGCACGCGCGACCTCACGCGCCTGCTGCCGCCGACGTCGCGTTCG
ATGCGCCGCATTGGCAAGATCACGGGTCGGTCGGACGACATGTTGATCATCCGCGGTGTGAACGTGTTCCCGTCGCAGAT
CGAGGAACTGATCCTCAAGGCGCCCGCGCTGGCCCCGCAATACCAGCTCGTCGTCACGCGCGACGGCCATCTGGACAAGC
TCGAGGTGCGCGTCGAAGCGCGTCCGCAATCGTCTGGCCTGAGCGGCAATGACCGTGCGGCGCTGGAGCAGCAACTCAAG
GAGCAGATCAAGACCTACGTCGGCGTGACCACGCGCGTGCAGGTGCTCGATGCCGAAGGCATCGAGCGCACGAGTGTGGG
CAAGGCGCGGCGTGTGCTGGATATGCGGCCGAAAGTTACGCATGAGCAGCCGGTCGGGGCGATCTGA

Upstream 100 bases:

>100_bases
GCAAGCACATCTGACCCTTACCGAGCTTCGCTCGCAGTACCGTAGCCCCTCGCGCCGGCCCCCCAAAGCATGCGGCGCCA
CGATAACGATGGAGTAGACA

Downstream 100 bases:

>100_bases
CCTTCCGGGCTGGCAAGTCTTGCCGGCCCGCAATTCCAATCCGATTTGACAAGGACGATGGCGCCCGCCAAGAGGCGCCA
CGGCCCCCCTCGCGCCAAAG

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MVQRTSHPDELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKSFDAAGVHPDDLKSLSDLSKFPFLAKQDLRDNYP
FGMFAVPREKVARVHASSGTTGKPTVVGYTARDIDTWASVVARSIRAAGGRPGDLVHVSYGYGLFTGGLGAHYGAEKAGC
TVIPMSGGQTEKQVQLIREFQPNIIMVTPSYMLNLIEEMMRQGMDPAESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI
YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEALPVIRYRTRDLTRLLPPTSRS
MRRIGKITGRSDDMLIIRGVNVFPSQIEELILKAPALAPQYQLVVTRDGHLDKLEVRVEARPQSSGLSGNDRAALEQQLK
EQIKTYVGVTTRVQVLDAEGIERTSVGKARRVLDMRPKVTHEQPVGAI

Sequences:

>Translated_448_residues
MVQRTSHPDELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKSFDAAGVHPDDLKSLSDLSKFPFLAKQDLRDNYP
FGMFAVPREKVARVHASSGTTGKPTVVGYTARDIDTWASVVARSIRAAGGRPGDLVHVSYGYGLFTGGLGAHYGAEKAGC
TVIPMSGGQTEKQVQLIREFQPNIIMVTPSYMLNLIEEMMRQGMDPAESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI
YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEALPVIRYRTRDLTRLLPPTSRS
MRRIGKITGRSDDMLIIRGVNVFPSQIEELILKAPALAPQYQLVVTRDGHLDKLEVRVEARPQSSGLSGNDRAALEQQLK
EQIKTYVGVTTRVQVLDAEGIERTSVGKARRVLDMRPKVTHEQPVGAI
>Mature_448_residues
MVQRTSHPDELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKSFDAAGVHPDDLKSLSDLSKFPFLAKQDLRDNYP
FGMFAVPREKVARVHASSGTTGKPTVVGYTARDIDTWASVVARSIRAAGGRPGDLVHVSYGYGLFTGGLGAHYGAEKAGC
TVIPMSGGQTEKQVQLIREFQPNIIMVTPSYMLNLIEEMMRQGMDPAESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI
YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEALPVIRYRTRDLTRLLPPTSRS
MRRIGKITGRSDDMLIIRGVNVFPSQIEELILKAPALAPQYQLVVTRDGHLDKLEVRVEARPQSSGLSGNDRAALEQQLK
EQIKTYVGVTTRVQVLDAEGIERTSVGKARRVLDMRPKVTHEQPVGAI

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=428, Percent_Identity=68.6915887850467, Blast_Score=610, Evalue=1e-176,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 49517; Mature: 49517

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQRTSHPDELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKSFDAAGVHPDDLKS
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHH
LSDLSKFPFLAKQDLRDNYPFGMFAVPREKVARVHASSGTTGKPTVVGYTARDIDTWASV
HHHHHHCCCHHHHHHCCCCCCCEEECCHHHHHHEECCCCCCCCCEEEEEEHHHHHHHHHH
VARSIRAAGGRPGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF
HHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHC
QPNIIMVTPSYMLNLIEEMMRQGMDPAESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI
CCCEEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEC
YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEAL
CCHHHHHCCCCEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHH
PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKAPALAPQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCC
YQLVVTRDGHLDKLEVRVEARPQSSGLSGNDRAALEQQLKEQIKTYVGVTTRVQVLDAEG
EEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
IERTSVGKARRVLDMRPKVTHEQPVGAI
CCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MVQRTSHPDELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKSFDAAGVHPDDLKS
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHH
LSDLSKFPFLAKQDLRDNYPFGMFAVPREKVARVHASSGTTGKPTVVGYTARDIDTWASV
HHHHHHCCCHHHHHHCCCCCCCEEECCHHHHHHEECCCCCCCCCEEEEEEHHHHHHHHHH
VARSIRAAGGRPGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF
HHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHC
QPNIIMVTPSYMLNLIEEMMRQGMDPAESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI
CCCEEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEC
YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEAL
CCHHHHHCCCCEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHH
PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKAPALAPQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCC
YQLVVTRDGHLDKLEVRVEARPQSSGLSGNDRAALEQQLKEQIKTYVGVTTRVQVLDAEG
EEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
IERTSVGKARRVLDMRPKVTHEQPVGAI
CCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]