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The map label for this gene is amnC [H]

Identifier: 73537575

GI number: 73537575

Start: 332597

End: 334261

Strand: Direct

Name: amnC [H]

Synonym: Reut_B3740

Alternate gene names: 73537575

Gene position: 332597-334261 (Clockwise)

Preceding gene: 73537574

Following gene: 73537576

Centisome position: 12.2

GC content: 65.89

Gene sequence:

>1665_bases
GTGTCCCATCCGTTTTTCACGCGTCATCAGGCGCTGCTGGACCAAGCCACCCAGGCCATCGCCGAGCGCGGCTACTGGAG
CCCGTTCCCCGAAATGCCGAGCCCCAGGGCGTACGGCGAGACCGCCAACGCCGACGGCAAGGCCGCATTCGAAGCGCGGC
TGAACAAGCCCTTCGCGCTGACACAGCCGGGCACGGTCGGCCAGGCCGGCAAGGAAGTCTCGCCGTATGGCACGGAACTG
GGCGTTACCTATCCGAAGCCCGATCTCGACCAGCTTTTCGCCGGCGTGGCGGCAGGCATGCCCGCATGGCGCAAGGCTGG
CCCCGAGGCTTGGGTTGGCGTCTCGCTCGAGATCCTGCATCGCCTGAACCGCCGCAGCTTCGAGATTGCGTACGCAGTCA
TGCACACCACCGGCCAGGCCTTCATGATGGCCTTCCAGGCCGGCGGCCCGCACGCGCAGGACCGCGGCCTGGAAGCCGTG
GCCTATGCGTGGGACGAGATGCGTCGCATCCCGAAGCGTGCAGTGTGGGAGAAGCCGCAAGGCAAGAACGAGCCGCTGCG
CATGGAGAAGCAGTACACCGTGGTGCCGCGTGGTGTCGGCCTGGTGATCGGCTGCTGCACGTTCCCGACGTGGAACGGAT
ATCCGGGCCTGTTCGCGAGCCTTGCCACCGGCAACGCCGTGGTGGTCAAGCCGCACCCGGGCGCGATCCTGCCGCTCGCC
ATCACCGTGGAAGTCGCGCGTGAAGTGCTGCAGGAAGCCGGCTTCGACCCGAACCTCGTCACGCTGGTCGCCAACGACCC
GACCGAGCGCATGGCATCGACGCTCGCGCTGCGTCCGGAAGTGCGCCTGATCGATTTCACCGGCAGCACCGCCAACGGTG
ACTGGCTCGAGCGCAATGCGCACCAGGCGCAGGTGTACACCGAGAAGGCTGGCGTGAACCAGATCGTCATCGACTCGACC
AGCGACTTCAAGGGCATGGTGCGCAATATCGCGTTCTCGCTCGCGCTGTACTCTGGCCAGATGTGCACGGCGCCGCAGAA
CATCTATGTGCCAAAGGGCGGCATCGACACGCCGGAAGGGCACCTGAGCTTCGATCAGGTCGCCAGCGCGATTGGCGAAG
TCGTGGGCAAGCTGACGGGCGATGCTGCCAAGGCCGTGGAACTGATCGGCGCGATCCAGAATGATGGCGTGGTGCAGCGT
ATCGAGGCATCTCGGTCGCTGGGCGAGATCGTCATCGACAGCCGCGCGCTGCAGCATCCCGAGTTCCCCGGCGCCCGCGT
GCACACGCCGCTGGTGCTCAAAGTCGATGCAAGCGACGAAGGCAAGATCATGCAGGAGCTGTTCGGTCCGATCTCGTTCG
TGGTCGCCACTGAAGACACCGCACACAGCATCGATCTGGCTCGCCGCGGCGCAAAGGAACACGGCGCGCTGACGCTGTCT
GCCTACACCACCAACGACGGCGTGGCCGATGCGATCAGCGATGCAGCGGAAGAGGCCGGCGTGGCGCTGTCGTTCAACCT
GACCGGCGCCGTGTTCGTCAATCAGTCGGCGGCATTCAGCGATTTCCATGCCACCGGCGCCAATCCTGCCGCCAATGCGT
CGCTGTCAGACGCGGCGTTCGTCGCCAACCGCTTCCGCGTGGTGCAGAGCCGCAAGCACATCTGA

Upstream 100 bases:

>100_bases
GACAAGCTGGTGATCGATTTCGACCAGGAAACCTGAGCGGTTCCCGGCGTCCATTCAAGTCATTCAAGCCATTCAAGCAA
ACATTTCCCCGGAGCAAGCT

Downstream 100 bases:

>100_bases
CCCTTACCGAGCTTCGCTCGCAGTACCGTAGCCCCTCGCGCCGGCCCCCCAAAGCATGCGGCGCCACGATAACGATGGAG
TAGACAATGGTTCAACGCAC

Product: phenylacetic acid degradation protein paaN2

Products: NA

Alternate protein names: Aminomuconate-semialdehyde dehydrogenase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSHPFFTRHQALLDQATQAIAERGYWSPFPEMPSPRAYGETANADGKAAFEARLNKPFALTQPGTVGQAGKEVSPYGTEL
GVTYPKPDLDQLFAGVAAGMPAWRKAGPEAWVGVSLEILHRLNRRSFEIAYAVMHTTGQAFMMAFQAGGPHAQDRGLEAV
AYAWDEMRRIPKRAVWEKPQGKNEPLRMEKQYTVVPRGVGLVIGCCTFPTWNGYPGLFASLATGNAVVVKPHPGAILPLA
ITVEVAREVLQEAGFDPNLVTLVANDPTERMASTLALRPEVRLIDFTGSTANGDWLERNAHQAQVYTEKAGVNQIVIDST
SDFKGMVRNIAFSLALYSGQMCTAPQNIYVPKGGIDTPEGHLSFDQVASAIGEVVGKLTGDAAKAVELIGAIQNDGVVQR
IEASRSLGEIVIDSRALQHPEFPGARVHTPLVLKVDASDEGKIMQELFGPISFVVATEDTAHSIDLARRGAKEHGALTLS
AYTTNDGVADAISDAAEEAGVALSFNLTGAVFVNQSAAFSDFHATGANPAANASLSDAAFVANRFRVVQSRKHI

Sequences:

>Translated_554_residues
MSHPFFTRHQALLDQATQAIAERGYWSPFPEMPSPRAYGETANADGKAAFEARLNKPFALTQPGTVGQAGKEVSPYGTEL
GVTYPKPDLDQLFAGVAAGMPAWRKAGPEAWVGVSLEILHRLNRRSFEIAYAVMHTTGQAFMMAFQAGGPHAQDRGLEAV
AYAWDEMRRIPKRAVWEKPQGKNEPLRMEKQYTVVPRGVGLVIGCCTFPTWNGYPGLFASLATGNAVVVKPHPGAILPLA
ITVEVAREVLQEAGFDPNLVTLVANDPTERMASTLALRPEVRLIDFTGSTANGDWLERNAHQAQVYTEKAGVNQIVIDST
SDFKGMVRNIAFSLALYSGQMCTAPQNIYVPKGGIDTPEGHLSFDQVASAIGEVVGKLTGDAAKAVELIGAIQNDGVVQR
IEASRSLGEIVIDSRALQHPEFPGARVHTPLVLKVDASDEGKIMQELFGPISFVVATEDTAHSIDLARRGAKEHGALTLS
AYTTNDGVADAISDAAEEAGVALSFNLTGAVFVNQSAAFSDFHATGANPAANASLSDAAFVANRFRVVQSRKHI
>Mature_553_residues
SHPFFTRHQALLDQATQAIAERGYWSPFPEMPSPRAYGETANADGKAAFEARLNKPFALTQPGTVGQAGKEVSPYGTELG
VTYPKPDLDQLFAGVAAGMPAWRKAGPEAWVGVSLEILHRLNRRSFEIAYAVMHTTGQAFMMAFQAGGPHAQDRGLEAVA
YAWDEMRRIPKRAVWEKPQGKNEPLRMEKQYTVVPRGVGLVIGCCTFPTWNGYPGLFASLATGNAVVVKPHPGAILPLAI
TVEVAREVLQEAGFDPNLVTLVANDPTERMASTLALRPEVRLIDFTGSTANGDWLERNAHQAQVYTEKAGVNQIVIDSTS
DFKGMVRNIAFSLALYSGQMCTAPQNIYVPKGGIDTPEGHLSFDQVASAIGEVVGKLTGDAAKAVELIGAIQNDGVVQRI
EASRSLGEIVIDSRALQHPEFPGARVHTPLVLKVDASDEGKIMQELFGPISFVVATEDTAHSIDLARRGAKEHGALTLSA
YTTNDGVADAISDAAEEAGVALSFNLTGAVFVNQSAAFSDFHATGANPAANASLSDAAFVANRFRVVQSRKHI

Specific function: Involved in the modified meta-cleavage pathway for the 2-aminophenol catabolism. The enzyme is also active toward 2- hydroxymuconic 6-semialdehyde, acetaldehyde, propionaldehyde, and butyraldehyde [H]

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldehyde dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4502043, Length=343, Percent_Identity=24.7813411078717, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI238814371, Length=343, Percent_Identity=24.7813411078717, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1787684, Length=345, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081896, Length=300, Percent_Identity=28.3333333333333, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787250, Length=369, Percent_Identity=26.5582655826558, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71995606, Length=274, Percent_Identity=28.4671532846715, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71995613, Length=274, Percent_Identity=28.4671532846715, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321828, Length=288, Percent_Identity=28.125, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323822, Length=300, Percent_Identity=28, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323821, Length=314, Percent_Identity=26.1146496815287, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016160
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR017628 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.32 [H]

Molecular weight: Translated: 59389; Mature: 59258

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPFFTRHQALLDQATQAIAERGYWSPFPEMPSPRAYGETANADGKAAFEARLNKPFAL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEE
TQPGTVGQAGKEVSPYGTELGVTYPKPDLDQLFAGVAAGMPAWRKAGPEAWVGVSLEILH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCEECCHHHHHH
RLNRRSFEIAYAVMHTTGQAFMMAFQAGGPHAQDRGLEAVAYAWDEMRRIPKRAVWEKPQ
HHCCCCCEEEEEEEHHCCHHHEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCC
GKNEPLRMEKQYTVVPRGVGLVIGCCTFPTWNGYPGLFASLATGNAVVVKPHPGAILPLA
CCCCCEEECCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHCCCCEEEECCCCCCEEEHH
ITVEVAREVLQEAGFDPNLVTLVANDPTERMASTLALRPEVRLIDFTGSTANGDWLERNA
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCC
HQAQVYTEKAGVNQIVIDSTSDFKGMVRNIAFSLALYSGQMCTAPQNIYVPKGGIDTPEG
CHHEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCC
HLSFDQVASAIGEVVGKLTGDAAKAVELIGAIQNDGVVQRIEASRSLGEIVIDSRALQHP
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHCCC
EFPGARVHTPLVLKVDASDEGKIMQELFGPISFVVATEDTAHSIDLARRGAKEHGALTLS
CCCCCCCCCCEEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEEE
AYTTNDGVADAISDAAEEAGVALSFNLTGAVFVNQSAAFSDFHATGANPAANASLSDAAF
EEECCCCHHHHHHHHHHHCCEEEEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
VANRFRVVQSRKHI
HHHHHHHHHHCCCC
>Mature Secondary Structure 
SHPFFTRHQALLDQATQAIAERGYWSPFPEMPSPRAYGETANADGKAAFEARLNKPFAL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEE
TQPGTVGQAGKEVSPYGTELGVTYPKPDLDQLFAGVAAGMPAWRKAGPEAWVGVSLEILH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCEECCHHHHHH
RLNRRSFEIAYAVMHTTGQAFMMAFQAGGPHAQDRGLEAVAYAWDEMRRIPKRAVWEKPQ
HHCCCCCEEEEEEEHHCCHHHEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCC
GKNEPLRMEKQYTVVPRGVGLVIGCCTFPTWNGYPGLFASLATGNAVVVKPHPGAILPLA
CCCCCEEECCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHCCCCEEEECCCCCCEEEHH
ITVEVAREVLQEAGFDPNLVTLVANDPTERMASTLALRPEVRLIDFTGSTANGDWLERNA
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCC
HQAQVYTEKAGVNQIVIDSTSDFKGMVRNIAFSLALYSGQMCTAPQNIYVPKGGIDTPEG
CHHEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCC
HLSFDQVASAIGEVVGKLTGDAAKAVELIGAIQNDGVVQRIEASRSLGEIVIDSRALQHP
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHCCC
EFPGARVHTPLVLKVDASDEGKIMQELFGPISFVVATEDTAHSIDLARRGAKEHGALTLS
CCCCCCCCCCEEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEEE
AYTTNDGVADAISDAAEEAGVALSFNLTGAVFVNQSAAFSDFHATGANPAANASLSDAAF
EEECCCCHHHHHHHHHHHCCEEEEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
VANRFRVVQSRKHI
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9169437; 11081795 [H]