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The map label for this gene is paaE [H]

Identifier: 73537574

GI number: 73537574

Start: 331456

End: 332532

Strand: Direct

Name: paaE [H]

Synonym: Reut_B3739

Alternate gene names: 73537574

Gene position: 331456-332532 (Clockwise)

Preceding gene: 73537573

Following gene: 73537575

Centisome position: 12.16

GC content: 63.97

Gene sequence:

>1077_bases
ATGAGCAAATTCCATGAACTGACCGTCGCCAGCGTGACACGCGAAACGCGCGATGCCGTGGCGGTGACTTTCGCCGTGCC
GGAGGACCTGGCCGATACCTATCGCTACGTGCAGGGCCAGCATCTGACGCTGCGCGCCGGCATCGGCGGCGAGGACGTCC
GCCGCTCGTACTCGATCTGCTCCGCCGTGCAGGACCGGCGCCTGCGCGTGGCGATCAAGCGCGTGGATGGCGGCATGTTC
TCGAACTGGGCCAACGATGCGTTGCAGCCGGGCATGACGCTGGAAGTCATGCCGCCGTCGGGACATTTCCACGTGCCGCT
GTCGGACACGCACAGCAAGCACTACGTGGCGTTCGCCGCCGGCAGCGGCATCACGCCGATGCTGTCGATCATCAAGACCA
CGCTGATGACCGAGCCGGACAGCCGCTTCACGCTGTTCTACGGCAACCGCGCATCGTCGTCGGTGCTGTTCAAGGAAGAA
CTTGAAGACCTGAAGGACACCTACCTCGAGCGCTTCAACCTCGTCTTCATCCTGAGCCGCGAACAGCTCGACATCGACCT
GTTCAATGGCCGCATCGACGGCGAGAAGGTGCGCGCGCTGCTGCGCCACTGGGTGCGCCCGCAGGACATCGACGTGGCCT
TTATCTGCGGCCCGCATTCGATGATGGAAGAGGTGTCGCAGGCGCTGCAGGAAAACGGCGTCGACAAGGCACGCATCAAG
CGCGAACTGTTCGCCACCAGCATTCCCGCGGCGCGCCCCGCGGCGCACGCACACAAGCAGCTGGGGCAGCAGCAGTGCGA
GGTCACGGTGATCCAGGATGGCCGCACGCGGCAGTTCACGCTGGAGAAGAACAAGGAGACCATCCTCGACGCCGCGCTGG
CGCAGGGGATCGAGCTGCCTTACTCCTGCAAGGGCGGCGTCTGTTCCACGTGCCGCTGCAAGCGCATCGAAGGCGAGGTC
GACATGGATGTCAACTTCGCGCTCGAAGACTACGAGGTTGCGCGCGGTTTCATCCTGAGCTGCCAGAGCTACGCCGTCAC
CGACAAGCTGGTGATCGATTTCGACCAGGAAACCTGA

Upstream 100 bases:

>100_bases
CCAGTTCGGCTCGACCGCCTGCAAGGCCCTGTACCGCTGCGGCGATTGCAAGGAACCTTTCGACTATTTCAAGGCCCATT
GAGCCGCCCGGAGCCGCAGA

Downstream 100 bases:

>100_bases
GCGGTTCCCGGCGTCCATTCAAGTCATTCAAGCCATTCAAGCAAACATTTCCCCGGAGCAAGCTGTGTCCCATCCGTTTT
TCACGCGTCATCAGGCGCTG

Product: phenylacetate-CoA oxygenase/reductase subunit PaaK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSKFHELTVASVTRETRDAVAVTFAVPEDLADTYRYVQGQHLTLRAGIGGEDVRRSYSICSAVQDRRLRVAIKRVDGGMF
SNWANDALQPGMTLEVMPPSGHFHVPLSDTHSKHYVAFAAGSGITPMLSIIKTTLMTEPDSRFTLFYGNRASSSVLFKEE
LEDLKDTYLERFNLVFILSREQLDIDLFNGRIDGEKVRALLRHWVRPQDIDVAFICGPHSMMEEVSQALQENGVDKARIK
RELFATSIPAARPAAHAHKQLGQQQCEVTVIQDGRTRQFTLEKNKETILDAALAQGIELPYSCKGGVCSTCRCKRIEGEV
DMDVNFALEDYEVARGFILSCQSYAVTDKLVIDFDQET

Sequences:

>Translated_358_residues
MSKFHELTVASVTRETRDAVAVTFAVPEDLADTYRYVQGQHLTLRAGIGGEDVRRSYSICSAVQDRRLRVAIKRVDGGMF
SNWANDALQPGMTLEVMPPSGHFHVPLSDTHSKHYVAFAAGSGITPMLSIIKTTLMTEPDSRFTLFYGNRASSSVLFKEE
LEDLKDTYLERFNLVFILSREQLDIDLFNGRIDGEKVRALLRHWVRPQDIDVAFICGPHSMMEEVSQALQENGVDKARIK
RELFATSIPAARPAAHAHKQLGQQQCEVTVIQDGRTRQFTLEKNKETILDAALAQGIELPYSCKGGVCSTCRCKRIEGEV
DMDVNFALEDYEVARGFILSCQSYAVTDKLVIDFDQET
>Mature_357_residues
SKFHELTVASVTRETRDAVAVTFAVPEDLADTYRYVQGQHLTLRAGIGGEDVRRSYSICSAVQDRRLRVAIKRVDGGMFS
NWANDALQPGMTLEVMPPSGHFHVPLSDTHSKHYVAFAAGSGITPMLSIIKTTLMTEPDSRFTLFYGNRASSSVLFKEEL
EDLKDTYLERFNLVFILSREQLDIDLFNGRIDGEKVRALLRHWVRPQDIDVAFICGPHSMMEEVSQALQENGVDKARIKR
ELFATSIPAARPAAHAHKQLGQQQCEVTVIQDGRTRQFTLEKNKETILDAALAQGIELPYSCKGGVCSTCRCKRIEGEVD
MDVNFALEDYEVARGFILSCQSYAVTDKLVIDFDQET

Specific function: May be part of a multicomponent oxygenase involved in phenylacetyl-CoA hydroxylation [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI49574502, Length=210, Percent_Identity=28.0952380952381, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI284448553, Length=212, Percent_Identity=28.7735849056604, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI6552328, Length=212, Percent_Identity=28.7735849056604, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI193794826, Length=212, Percent_Identity=28.7735849056604, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI284448551, Length=212, Percent_Identity=28.7735849056604, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI4503327, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787658, Length=355, Percent_Identity=50.4225352112676, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI1787098, Length=343, Percent_Identity=25.0728862973761, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788903, Length=239, Percent_Identity=25.5230125523013, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17563926, Length=210, Percent_Identity=27.1428571428571, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322146, Length=240, Percent_Identity=25, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR011884
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40132; Mature: 40001

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFHELTVASVTRETRDAVAVTFAVPEDLADTYRYVQGQHLTLRAGIGGEDVRRSYSIC
CCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHH
SAVQDRRLRVAIKRVDGGMFSNWANDALQPGMTLEVMPPSGHFHVPLSDTHSKHYVAFAA
HHHHHHHHEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCCEEEEEEC
GSGITPMLSIIKTTLMTEPDSRFTLFYGNRASSSVLFKEELEDLKDTYLERFNLVFILSR
CCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCEEEEEEC
EQLDIDLFNGRIDGEKVRALLRHWVRPQDIDVAFICGPHSMMEEVSQALQENGVDKARIK
CCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHH
RELFATSIPAARPAAHAHKQLGQQQCEVTVIQDGRTRQFTLEKNKETILDAALAQGIELP
HHHHHHCCCCCCCHHHHHHHHCHHHCEEEEEECCCCEEEEEECCHHHHHHHHHHHCCCCC
YSCKGGVCSTCRCKRIEGEVDMDVNFALEDYEVARGFILSCQSYAVTDKLVIDFDQET
CCCCCCCCCCCCCEECCCCCCCEEEEEECHHHHHHHHEEECCCEEEEEEEEEEECCCC
>Mature Secondary Structure 
SKFHELTVASVTRETRDAVAVTFAVPEDLADTYRYVQGQHLTLRAGIGGEDVRRSYSIC
CCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHH
SAVQDRRLRVAIKRVDGGMFSNWANDALQPGMTLEVMPPSGHFHVPLSDTHSKHYVAFAA
HHHHHHHHEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCCEEEEEEC
GSGITPMLSIIKTTLMTEPDSRFTLFYGNRASSSVLFKEELEDLKDTYLERFNLVFILSR
CCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCEEEEEEC
EQLDIDLFNGRIDGEKVRALLRHWVRPQDIDVAFICGPHSMMEEVSQALQENGVDKARIK
CCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHH
RELFATSIPAARPAAHAHKQLGQQQCEVTVIQDGRTRQFTLEKNKETILDAALAQGIELP
HHHHHHCCCCCCCHHHHHHHHCHHHCEEEEEECCCCEEEEEECCHHHHHHHHHHHCCCCC
YSCKGGVCSTCRCKRIEGEVDMDVNFALEDYEVARGFILSCQSYAVTDKLVIDFDQET
CCCCCCCCCCCCCEECCCCCCCEEEEEECHHHHHHHHEEECCCEEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]