Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 73537559

GI number: 73537559

Start: 314269

End: 315612

Strand: Direct

Name: gdhA [H]

Synonym: Reut_B3724

Alternate gene names: 73537559

Gene position: 314269-315612 (Clockwise)

Preceding gene: 73537557

Following gene: 73537560

Centisome position: 11.53

GC content: 64.21

Gene sequence:

>1344_bases
GTGAATTCTCCCTCCCTCGACGCATTCCTTGCCGGCGTCGCCCGGCGCGACCCCGGCCAACCTGAATTTCTGCAGGCCGT
GAAGGAAGTCATGATGACGCTCTGGCCGTTTGTCGAGCGTCACCCGCGCTACGCCGATCAGGCCCTGCTCGAACGCCTGG
TCGAGCCCGAGCGTGTGATCCAGTTCCGCGTGGCGTGGACCGACGACCAGAACCGCGTGCAGGTCAACCGCGCGTTCCGC
GTGCAGCACAGCTCGGCCATCGGCCCGTACAAGGGCGGCATGCGTTTCCACCCGACGGTGAACCTGTCGGTGCTCAAGTT
CCTCGGTTTCGAGCAGACCTTCAAGAACGCACTGACCACGCTGCCGATGGGCGGTGGCAAGGGCGGTTCGGATTTCGACC
CGAAGGGCAAGTCGGACGCTGAAGTGATGCGCTTCTGCCAGGCGCTGGTCACCGAGCTGTACCGCCACCTGGGGCCGGAC
ACCGACATCCCCGCCGGCGACATCGGCGTGGGCGCGCGCGAGGTCGGCTTCATGGCCGGTATGATGAAGAAGCTGTCGAA
CCAGTCGGCCTGCGTGTTCACCGGCAAGGGCCTGGCGTTCGGCGGCAGCCTGATGCGCCCCGAAGCCACGGGCTACGGCA
CCGTGTACTTCGCCCAGGAAATGCTCCACCGCCGTGGCATGGACTTCGACGGCCTGCGCGTGCTGCTGTCCGGCTCGGGC
AACGTGGCGCAGTACGCAGCCGAGAAGGCCATCGAACTGGGTGCCAAGGTGCTGACGCTGACCGACTCCGGCGGCGTGCT
GCACTACCCGGAGGGGATGACCACAGAGCAACTGGCCGAAGTGATGGCGTTCAAGAACGAAGAGCGTGGTCGACTGTCCG
ACTTTGCCGCACGCCATGGCATGAAGTTCGAAGCCGGCCGCACGCCGTGGCATGTGCCTGCCGACGTAGCGCTGCCTTGC
GCAACGCAGAACGAACTCGATGGCAACGACGCCGAGACCCTGCTTGGCAACGGTGTGATCTGCGTGGCCGAAGGTGCGAA
CATGCCCTCGACGCTCGAAGCCGTGGACCGCTTCGTCGACGCGAAAATCCTCTACGCACCCGGCAAGGCCAGCAATGCCG
GTGGCGTGGCGACCTCAGGTCTGGAAATGTCGCAGAATGCCATGCGCCTGTCCTGGCACCATGCCGAGGTCGACGAGAAG
TTGCACGCGATCATGAAGGACATCCACCAGAACTGCATTCACCATGGGCAGAAGGATGGCGGGTACGTCAACTATGTCGA
AGGGGCGAATATCGCTGGCTTTGTGAAGGTGGCGGATGCGATGTTGGCGCAAGGGGTGATTTGA

Upstream 100 bases:

>100_bases
GTGCCGCCCAGGGTTTTCCACGGGTCGCCGCCATTCTCACGGTACGCAGCCCGGCACGTATCCGGCGCGGCCGCCTTTTC
CAGCACAGCAGAGACATCCC

Downstream 100 bases:

>100_bases
CGGCTGTGACTGCGGAGTGTGTTGCCGGGATGCCGTGCCCGGCATACACCGCTGTTTCGCCGGTGTAGGCCGCGACCTGC
CTCTTGTCCGAGCGACAAGA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNSPSLDAFLAGVARRDPGQPEFLQAVKEVMMTLWPFVERHPRYADQALLERLVEPERVIQFRVAWTDDQNRVQVNRAFR
VQHSSAIGPYKGGMRFHPTVNLSVLKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQALVTELYRHLGPD
TDIPAGDIGVGAREVGFMAGMMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRRGMDFDGLRVLLSGSG
NVAQYAAEKAIELGAKVLTLTDSGGVLHYPEGMTTEQLAEVMAFKNEERGRLSDFAARHGMKFEAGRTPWHVPADVALPC
ATQNELDGNDAETLLGNGVICVAEGANMPSTLEAVDRFVDAKILYAPGKASNAGGVATSGLEMSQNAMRLSWHHAEVDEK
LHAIMKDIHQNCIHHGQKDGGYVNYVEGANIAGFVKVADAMLAQGVI

Sequences:

>Translated_447_residues
MNSPSLDAFLAGVARRDPGQPEFLQAVKEVMMTLWPFVERHPRYADQALLERLVEPERVIQFRVAWTDDQNRVQVNRAFR
VQHSSAIGPYKGGMRFHPTVNLSVLKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQALVTELYRHLGPD
TDIPAGDIGVGAREVGFMAGMMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRRGMDFDGLRVLLSGSG
NVAQYAAEKAIELGAKVLTLTDSGGVLHYPEGMTTEQLAEVMAFKNEERGRLSDFAARHGMKFEAGRTPWHVPADVALPC
ATQNELDGNDAETLLGNGVICVAEGANMPSTLEAVDRFVDAKILYAPGKASNAGGVATSGLEMSQNAMRLSWHHAEVDEK
LHAIMKDIHQNCIHHGQKDGGYVNYVEGANIAGFVKVADAMLAQGVI
>Mature_447_residues
MNSPSLDAFLAGVARRDPGQPEFLQAVKEVMMTLWPFVERHPRYADQALLERLVEPERVIQFRVAWTDDQNRVQVNRAFR
VQHSSAIGPYKGGMRFHPTVNLSVLKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQALVTELYRHLGPD
TDIPAGDIGVGAREVGFMAGMMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRRGMDFDGLRVLLSGSG
NVAQYAAEKAIELGAKVLTLTDSGGVLHYPEGMTTEQLAEVMAFKNEERGRLSDFAARHGMKFEAGRTPWHVPADVALPC
ATQNELDGNDAETLLGNGVICVAEGANMPSTLEAVDRFVDAKILYAPGKASNAGGVATSGLEMSQNAMRLSWHHAEVDEK
LHAIMKDIHQNCIHHGQKDGGYVNYVEGANIAGFVKVADAMLAQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=405, Percent_Identity=27.6543209876543, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI4885281, Length=405, Percent_Identity=27.4074074074074, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1788059, Length=443, Percent_Identity=69.7516930022573, Blast_Score=650, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=341, Percent_Identity=28.1524926686217, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=49.7787610619469, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=49.7797356828194, Blast_Score=396, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45549226, Length=356, Percent_Identity=28.0898876404494, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI45553475, Length=356, Percent_Identity=28.0898876404494, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24649283, Length=334, Percent_Identity=29.0419161676647, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24649460, Length=295, Percent_Identity=28.135593220339, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48508; Mature: 48508

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00074 GLFV_DEHYDROGENASE ; PS00436 PEROXIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPSLDAFLAGVARRDPGQPEFLQAVKEVMMTLWPFVERHPRYADQALLERLVEPERVI
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
QFRVAWTDDQNRVQVNRAFRVQHSSAIGPYKGGMRFHPTVNLSVLKFLGFEQTFKNALTT
EEEEEEECCCCEEEEEHHEEEECCCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHHH
LPMGGGKGGSDFDPKGKSDAEVMRFCQALVTELYRHLGPDTDIPAGDIGVGAREVGFMAG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
MMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRRGMDFDGLRVLLSGSG
HHHHHCCCCEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
NVAQYAAEKAIELGAKVLTLTDSGGVLHYPEGMTTEQLAEVMAFKNEERGRLSDFAARHG
CHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCC
MKFEAGRTPWHVPADVALPCATQNELDGNDAETLLGNGVICVAEGANMPSTLEAVDRFVD
CEECCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
AKILYAPGKASNAGGVATSGLEMSQNAMRLSWHHAEVDEKLHAIMKDIHQNCIHHGQKDG
CEEEECCCCCCCCCCCEECCCHHCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GYVNYVEGANIAGFVKVADAMLAQGVI
CEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSPSLDAFLAGVARRDPGQPEFLQAVKEVMMTLWPFVERHPRYADQALLERLVEPERVI
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
QFRVAWTDDQNRVQVNRAFRVQHSSAIGPYKGGMRFHPTVNLSVLKFLGFEQTFKNALTT
EEEEEEECCCCEEEEEHHEEEECCCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHHH
LPMGGGKGGSDFDPKGKSDAEVMRFCQALVTELYRHLGPDTDIPAGDIGVGAREVGFMAG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
MMKKLSNQSACVFTGKGLAFGGSLMRPEATGYGTVYFAQEMLHRRGMDFDGLRVLLSGSG
HHHHHCCCCEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
NVAQYAAEKAIELGAKVLTLTDSGGVLHYPEGMTTEQLAEVMAFKNEERGRLSDFAARHG
CHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCC
MKFEAGRTPWHVPADVALPCATQNELDGNDAETLLGNGVICVAEGANMPSTLEAVDRFVD
CEECCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
AKILYAPGKASNAGGVATSGLEMSQNAMRLSWHHAEVDEKLHAIMKDIHQNCIHHGQKDG
CEEEECCCCCCCCCCCEECCCHHCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GYVNYVEGANIAGFVKVADAMLAQGVI
CEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]