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The map label for this gene is garD [H]

Identifier: 73537364

GI number: 73537364

Start: 75051

End: 76226

Strand: Direct

Name: garD [H]

Synonym: Reut_B3529

Alternate gene names: 73537364

Gene position: 75051-76226 (Clockwise)

Preceding gene: 73537363

Following gene: 73537365

Centisome position: 2.75

GC content: 64.63

Gene sequence:

>1176_bases
ATGTCCGTGATCGACCAAAACACCACCTTCTGGGGCTACCGCCGCGACAATGGCCGCGTTGGTGTGCGCAACCACGTCAT
CATCCTGCCGCTGGACGACCTGTCCAATGCCGCCGCCGAAGCCGTGGCCGCCTCCATCAAAGGCACTATGGCGATTCCGC
ACCCGTATGGCCGCCTGCAGTTTGGGCCGGACCTGGACCTGCACTTCCGCACGCTGATTGGTGCGGGCAGCAATCCGAAC
GTCGCCGCGGTGGTGGTGATCGGCATCGAAGACGGCTGGACCAAGCGTGTCGTCGACGGCATCGCGAAGACTGGCAAGCC
CGTGGTCGGTTTCGGCATCGAAGGCCACGGCGATCACCAGACCATCATGCGCGCCTCGAAGGCGGCCAAGGAATACGTGC
AGTACGCGACCGCGCTGCATCGCGTCGAATGCCCGATCAGCGAACTGTGGATATCGACCAAGTGCGGCGAGTCCGATACC
ACGTCCGGCTGCGGCGCCAACCCGACGGTCGGCAATGCGTTCGACAAGCTCCATCCGCTCGGCACGACGCTGGTGTTCGG
CGAGACGTCGGAACTGACCGGCGGCGAGCACATCGTCGCGGCGCGCTGCGTCAATGACACGGTGCGCCACGAGTTCATGG
CGATGTTCGAGCGCTACCAGGGCATGATTGACCGCTGGAAGACCTCGGACCTCTCGGAATCCCAGCCGACCAAGGGCAAT
ATCGCGGGCGGCCTGACCACGATCGAAGAGAAGGCGCTCGGCAATATCCAGAAGATCGGCAAGAAGTGCACGGTCGACGG
TGTGCTGGACAAGGCGGAGGAACCGACCCATCCGGGCCTGTGGTTCATGGATTCGTCGTCCGCCGCGGCTGAAATGGTGA
CGCTGTGCGCCGCCGCCGGCTACGTCGTGCACTTCTTCCCGACGGGGCAGGGCAACGTGATCGGCAACCCGATCGTCCCG
GTCATCAAGCTCTGCGCGAATCCGCGTACGGTGCGGCTGATGAGCGAGCACATGGACGTCGATTGCTCAGGCTTGCTGCA
ACGCGAACAGACGCTCGACCAGACCGGCGACAAGCTGCTCGAGTGCATGCTGGCCACGGCCAACGGGCGCTGGACCGCTG
CCGAGGCGCTCGGCCACCGCGAATTCGTGCTGACGCGCATCCACGAGTCCGCATGA

Upstream 100 bases:

>100_bases
GCACGCCCACGTCCACAACATCAAGACCAAGCGCTGGTAATTCGCCGCGCCGCTGTCACCCCATCCATCTGATCCCCATT
CCAAATCCGAGGGCCACGTC

Downstream 100 bases:

>100_bases
TGAAGCGCATCTGCATCAGCGAGTTCATGGACCCGGCCGCGGTGCAGGCGCTCACGCCCGGCTTCGACGTGCGCTACGAG
CCGGGCTGGGTGGACCAGCG

Product: Altronate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSVIDQNTTFWGYRRDNGRVGVRNHVIILPLDDLSNAAAEAVAASIKGTMAIPHPYGRLQFGPDLDLHFRTLIGAGSNPN
VAAVVVIGIEDGWTKRVVDGIAKTGKPVVGFGIEGHGDHQTIMRASKAAKEYVQYATALHRVECPISELWISTKCGESDT
TSGCGANPTVGNAFDKLHPLGTTLVFGETSELTGGEHIVAARCVNDTVRHEFMAMFERYQGMIDRWKTSDLSESQPTKGN
IAGGLTTIEEKALGNIQKIGKKCTVDGVLDKAEEPTHPGLWFMDSSSAAAEMVTLCAAAGYVVHFFPTGQGNVIGNPIVP
VIKLCANPRTVRLMSEHMDVDCSGLLQREQTLDQTGDKLLECMLATANGRWTAAEALGHREFVLTRIHESA

Sequences:

>Translated_391_residues
MSVIDQNTTFWGYRRDNGRVGVRNHVIILPLDDLSNAAAEAVAASIKGTMAIPHPYGRLQFGPDLDLHFRTLIGAGSNPN
VAAVVVIGIEDGWTKRVVDGIAKTGKPVVGFGIEGHGDHQTIMRASKAAKEYVQYATALHRVECPISELWISTKCGESDT
TSGCGANPTVGNAFDKLHPLGTTLVFGETSELTGGEHIVAARCVNDTVRHEFMAMFERYQGMIDRWKTSDLSESQPTKGN
IAGGLTTIEEKALGNIQKIGKKCTVDGVLDKAEEPTHPGLWFMDSSSAAAEMVTLCAAAGYVVHFFPTGQGNVIGNPIVP
VIKLCANPRTVRLMSEHMDVDCSGLLQREQTLDQTGDKLLECMLATANGRWTAAEALGHREFVLTRIHESA
>Mature_390_residues
SVIDQNTTFWGYRRDNGRVGVRNHVIILPLDDLSNAAAEAVAASIKGTMAIPHPYGRLQFGPDLDLHFRTLIGAGSNPNV
AAVVVIGIEDGWTKRVVDGIAKTGKPVVGFGIEGHGDHQTIMRASKAAKEYVQYATALHRVECPISELWISTKCGESDTT
SGCGANPTVGNAFDKLHPLGTTLVFGETSELTGGEHIVAARCVNDTVRHEFMAMFERYQGMIDRWKTSDLSESQPTKGNI
AGGLTTIEEKALGNIQKIGKKCTVDGVLDKAEEPTHPGLWFMDSSSAAAEMVTLCAAAGYVVHFFPTGQGNVIGNPIVPV
IKLCANPRTVRLMSEHMDVDCSGLLQREQTLDQTGDKLLECMLATANGRWTAAEALGHREFVLTRIHESA

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=385, Percent_Identity=29.8701298701299, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789475, Length=404, Percent_Identity=29.4554455445545, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 42167; Mature: 42036

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIDQNTTFWGYRRDNGRVGVRNHVIILPLDDLSNAAAEAVAASIKGTMAIPHPYGRLQ
CCCCCCCCEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCEECCCCCCCCC
FGPDLDLHFRTLIGAGSNPNVAAVVVIGIEDGWTKRVVDGIAKTGKPVVGFGIEGHGDHQ
CCCCCCEEEHEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCHH
TIMRASKAAKEYVQYATALHRVECPISELWISTKCGESDTTSGCGANPTVGNAFDKLHPL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHCCC
GTTLVFGETSELTGGEHIVAARCVNDTVRHEFMAMFERYQGMIDRWKTSDLSESQPTKGN
CCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
IAGGLTTIEEKALGNIQKIGKKCTVDGVLDKAEEPTHPGLWFMDSSSAAAEMVTLCAAAG
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHCC
YVVHFFPTGQGNVIGNPIVPVIKLCANPRTVRLMSEHMDVDCSGLLQREQTLDQTGDKLL
EEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHH
ECMLATANGRWTAAEALGHREFVLTRIHESA
HHHHHCCCCCEEHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SVIDQNTTFWGYRRDNGRVGVRNHVIILPLDDLSNAAAEAVAASIKGTMAIPHPYGRLQ
CCCCCCCEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCEECCCCCCCCC
FGPDLDLHFRTLIGAGSNPNVAAVVVIGIEDGWTKRVVDGIAKTGKPVVGFGIEGHGDHQ
CCCCCCEEEHEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCHH
TIMRASKAAKEYVQYATALHRVECPISELWISTKCGESDTTSGCGANPTVGNAFDKLHPL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHCCC
GTTLVFGETSELTGGEHIVAARCVNDTVRHEFMAMFERYQGMIDRWKTSDLSESQPTKGN
CCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
IAGGLTTIEEKALGNIQKIGKKCTVDGVLDKAEEPTHPGLWFMDSSSAAAEMVTLCAAAG
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHCC
YVVHFFPTGQGNVIGNPIVPVIKLCANPRTVRLMSEHMDVDCSGLLQREQTLDQTGDKLL
EEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHH
ECMLATANGRWTAAEALGHREFVLTRIHESA
HHHHHCCCCCEEHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]