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The map label for this gene is yfcG [H]

Identifier: 73537328

GI number: 73537328

Start: 29819

End: 30553

Strand: Direct

Name: yfcG [H]

Synonym: Reut_B3493

Alternate gene names: 73537328

Gene position: 29819-30553 (Clockwise)

Preceding gene: 73537326

Following gene: 73537329

Centisome position: 1.09

GC content: 62.18

Gene sequence:

>735_bases
ATGCCGTGCCGCCAGTTGCCAGGAGTTTTTGTGTCCGACCTGTCCGCCTATCCCATCGCCAGCAAATGGCCAGCGCAGCA
TCCCGACCGCATCCAGCTTTATTCGCTGCCCACGCCGAACGGCGTGAAAGTATCCACGATGCTGGAAGAGACCGGGCTGC
CCTACGAGCCGCACCTTGTGCGCTTCGACCACAACGACCAGTTGTCGCCCGAGTTCCTTTCGCTGAATCCCAATAACAAG
ATCCCGGCCATCATCGATCCCAACGGACCCGGCGCAAAGCCGCTGCCGCTGTGGGAATCCGGTGCCATCCTGCTGTACCT
GGCCGACAAGTCGGGGCAGCTGATTCCTGCCGATCCCGCGGAACGTTACGAGACCATCCAGTGGCTCATGTTCCAGATGG
GCGGCATCGGTCCCATGTTTGGCCAGGTGGGATTCTTCAACCGTTTTGCGGGCAAGGAGTACGAGGACAAGCGTCCGCGC
GACCGTTATGCGGCCGAGAGCCGCCGCTTGCTCAACGTGCTGAACCAGCGCCTGGCCGACCGGTCGTGGATCATGGGCGA
GCAATACACGATCGCCGACATCGCGACGTTCCCTTGGGTGCGCAATCTGGTGGGTTTCTATGAAGCGGGCGAACTCGTCG
GCATCGGCGATTTCCCGCATGTGACGCGCGCACTCGAAGCCTTCGTGGCCCGTCCCGCAGTAATCAAGGGCCTCGACACG
CCGCATCGCGGCTGA

Upstream 100 bases:

>100_bases
GATTTTCTTTTTGCTTTGATTCCGGTGTATCGCGCAATAAGGCCCCGGCCTGCAGCGCCTGCACGGGCGGTTTATCATCG
GACATTCAGGCGCGGCATTG

Downstream 100 bases:

>100_bases
GATACCGCCGCGTAGGCATCGCGGGGCTCGGCACATGTGTCGGGCAACGTCCTACAGCGCGCTACTTGCGCGCTGACAGA
GACGGGCAAGGCGCGCACCT

Product: glutathione S-transferase

Products: NA

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MPCRQLPGVFVSDLSAYPIASKWPAQHPDRIQLYSLPTPNGVKVSTMLEETGLPYEPHLVRFDHNDQLSPEFLSLNPNNK
IPAIIDPNGPGAKPLPLWESGAILLYLADKSGQLIPADPAERYETIQWLMFQMGGIGPMFGQVGFFNRFAGKEYEDKRPR
DRYAAESRRLLNVLNQRLADRSWIMGEQYTIADIATFPWVRNLVGFYEAGELVGIGDFPHVTRALEAFVARPAVIKGLDT
PHRG

Sequences:

>Translated_244_residues
MPCRQLPGVFVSDLSAYPIASKWPAQHPDRIQLYSLPTPNGVKVSTMLEETGLPYEPHLVRFDHNDQLSPEFLSLNPNNK
IPAIIDPNGPGAKPLPLWESGAILLYLADKSGQLIPADPAERYETIQWLMFQMGGIGPMFGQVGFFNRFAGKEYEDKRPR
DRYAAESRRLLNVLNQRLADRSWIMGEQYTIADIATFPWVRNLVGFYEAGELVGIGDFPHVTRALEAFVARPAVIKGLDT
PHRG
>Mature_243_residues
PCRQLPGVFVSDLSAYPIASKWPAQHPDRIQLYSLPTPNGVKVSTMLEETGLPYEPHLVRFDHNDQLSPEFLSLNPNNKI
PAIIDPNGPGAKPLPLWESGAILLYLADKSGQLIPADPAERYETIQWLMFQMGGIGPMFGQVGFFNRFAGKEYEDKRPRD
RYAAESRRLLNVLNQRLADRSWIMGEQYTIADIATFPWVRNLVGFYEAGELVGIGDFPHVTRALEAFVARPAVIKGLDTP
HRG

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=209, Percent_Identity=43.5406698564593, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI87082195, Length=222, Percent_Identity=42.3423423423423, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081789, Length=208, Percent_Identity=29.8076923076923, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324100, Length=282, Percent_Identity=32.2695035460993, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17933730, Length=201, Percent_Identity=29.8507462686567, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571670, Length=177, Percent_Identity=28.8135593220339, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17864592, Length=197, Percent_Identity=29.4416243654822, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549270, Length=197, Percent_Identity=29.9492385786802, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17864594, Length=198, Percent_Identity=30.8080808080808, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17864598, Length=179, Percent_Identity=29.608938547486, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24654983, Length=192, Percent_Identity=30.7291666666667, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI85725204, Length=170, Percent_Identity=31.1764705882353, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737923, Length=170, Percent_Identity=31.1764705882353, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 27373; Mature: 27241

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCRQLPGVFVSDLSAYPIASKWPAQHPDRIQLYSLPTPNGVKVSTMLEETGLPYEPHLV
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHCCCCCCCEEE
RFDHNDQLSPEFLSLNPNNKIPAIIDPNGPGAKPLPLWESGAILLYLADKSGQLIPADPA
EECCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCHH
ERYETIQWLMFQMGGIGPMFGQVGFFNRFAGKEYEDKRPRDRYAAESRRLLNVLNQRLAD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
RSWIMGEQYTIADIATFPWVRNLVGFYEAGELVGIGDFPHVTRALEAFVARPAVIKGLDT
CHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCHHHCCCCC
PHRG
CCCC
>Mature Secondary Structure 
PCRQLPGVFVSDLSAYPIASKWPAQHPDRIQLYSLPTPNGVKVSTMLEETGLPYEPHLV
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHCCCCCCCEEE
RFDHNDQLSPEFLSLNPNNKIPAIIDPNGPGAKPLPLWESGAILLYLADKSGQLIPADPA
EECCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCHH
ERYETIQWLMFQMGGIGPMFGQVGFFNRFAGKEYEDKRPRDRYAAESRRLLNVLNQRLAD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
RSWIMGEQYTIADIATFPWVRNLVGFYEAGELVGIGDFPHVTRALEAFVARPAVIKGLDT
CHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCHHHCCCCC
PHRG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]