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The map label for this gene is fusA1 [H]

Identifier: 73542867

GI number: 73542867

Start: 3482745

End: 3484853

Strand: Reverse

Name: fusA1 [H]

Synonym: Reut_A3183

Alternate gene names: 73542867

Gene position: 3484853-3482745 (Counterclockwise)

Preceding gene: 73542868

Following gene: 73542866

Centisome position: 91.55

GC content: 61.64

Gene sequence:

>2109_bases
GTGGCTCGTAAGACTCCTATTGAGCGCTACCGTAACATCGGTATTTCGGCTCACATTGACGCGGGTAAAACCACCACGAC
CGAGCGGATCCTGTTCTACACCGGTGTGAACCACAAGATCGGTGAAGTGCATGATGGCGCGGCCACCATGGACTGGATGG
AGCAGGAGCAGGAGCGCGGCATCACGATCACGTCTGCTGCGACCACGGCCTTCTGGAAGGGCATGGGCGGCAACTACCCC
GAGCACCGCTTCAACATCATCGACACCCCGGGACACGTGGACTTCACCATTGAGGTGGAGCGTTCCATGCGCGTGCTGGA
TGGCGCCTGCATGGTCTACTGTGCAGTGGGTGGCGTGCAGCCCCAGTCGGAAACCGTTTGGCGCCAGGCCAACAAGTACA
AGGTGCCGCGTCTGGCGTTCGTCAACAAGATGGACCGTACCGGCGCGAACTTCTTCAAGGTCTACGACCAGCTGAAGACC
CGCCTGAAGGCCAACCCGGTGCCCGTCGTAGTGCCGATCGGCGCTGAAGACGGCTTCCAGGGCGTCGTCGATCTGCTGGA
AATGAAGGCGATCATTTGGGACGAAGCCAGCCAGGGCGTGAAGTTCGAATACCAGGACATCCCGGCCGAACTGCAAGCCA
CCGCTGACGAATGGCGCGAGAAGATGGTCGAGTCCGCCGCCGAAGCCAGCGAAGAGCTGATGGAAAAGTACCTGGGCGGC
GAAGAGCTGACCCGCGCCGAGATCGTCAAGGCGCTGCGTGACCGTACCATCGCCTGCGAAATCCAGCCGATGCTGTGCGG
CACCGCGTTCAAGAACAAGGGCGTGCAGCGTATGCTCGACGCCGTGATCGACTTCCTGCCGTCGCCGGTCGACATTCCGC
CGGTTACGGGCACGGACGAGGACGACAGCGAGAAGAAGCTCGAGCGCAAGGCTGACGACACCGAAAAGTTCTCGGCACTG
GCGTTCAAGATCATGACCGACCCGTTCGTTGGTCAGCTGATCTTCTTCCGCGTCTACTCGGGCAAGATCAATTCGGGCGA
CACCGTGTACAACCCGGTGAAGCAGAAGAAGGAGCGTCTGGGCCGTATTCTGCAGATGCACGCCAACCAGCGCGAAGAAA
TCAAGGAAGTGCTGGCCGGCGACATCGCCGCTGCGGTGGGCCTGAAGGATGCCACCACGGGCGACACGCTGTGCGATCCG
GCTGCGCCGATCGTGCTCGAGCGCATGATCTTCCCTGAGCCGGTGATCTCGCAGGCTGTCGAGCCGAAGACCAAGGCTGA
CCAGGAAAAGATGGGCATCGCCCTGAACCGCCTGGCCGCTGAGGATCCGTCGTTCCGCGTGCGTACCGATGAAGAATCGG
GCCAGACCATCATTTCGGGTATGGGCGAGCTCCACCTCGAAATTCTGGTCGACCGCATGAAGCGCGAATTCGGCGTGGAA
GCCAACATCGGCGCGCCGCAGGTGGCCTACCGCGAAACCATTCGCAAGACGGCCGAAGGCGTCGAGGGCAAGTTCGTCAA
GCAGTCGGGCGGCCGCGGCCAGTACGGTCACGCCGTGATCACGCTGGAACCGCAAGAGCCGGGCAAGGGCTTCGAGTTCA
TCGACGCCATCAAGGGCGGTGTGATTCCTCGCGAATACATCCCGGCGGTCGAAAAGGGTATTGTCGACACGCTGCCGTCC
GGTATTCTGGCCGGCTTCCCGGTGGTGGACGTGAAGGTCACGCTGACGTTCGGTTCGTACCACGATGTGGACTCGAACGA
AAACGCGTTCCGCATGGCCGGCTCGATGGCTTTCAAGGAAGCCATGCGCAAGGCCAGCCCGGTTCTGCTCGAGCCGATGA
TGGCCGTGGAAGTGGAAACGCCGGAAGACTACACGGGTACCGTGATGGGCGATCTGTCGTCCCGCCGCGGCATCGTGCAG
GGCATGGACGACATGGTGGGCGGCGGCAAGATCATCAAGGCCGAAGTCCCGCTGTCGGAAATGTTCGGTTATTCCACGTC
GCTGCGCTCGGCCACGCAAGGTCGCGCCACGTACACGATGGAATTCAAGCACTACGCTGAGGCACCGAAGAACATCGCCG
AAGCCGTGATGGCGGCCAAGGGCAAGTAA

Upstream 100 bases:

>100_bases
TTGGTTGCCGGGCGGGCTGTGTTTTTACACATCTCGCCCGTTTGTGTTAAGGGCGCAGACCATGAGATTTCTGCGCCTCA
TGGACGAATAGAGGATTAAA

Downstream 100 bases:

>100_bases
TTGGAAGTTTGCCGCCGCGCCAGCCGGTGCGGCGGCGCGATGAAACAACAAGACTGCATTCCTATCAGGAGCTAAGAAAT
GGCAAAGGAAAAGTTCGAGC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 1 [H]

Number of amino acids: Translated: 702; Mature: 701

Protein sequence:

>702_residues
MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTAFWKGMGGNYP
EHRFNIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYDQLKT
RLKANPVPVVVPIGAEDGFQGVVDLLEMKAIIWDEASQGVKFEYQDIPAELQATADEWREKMVESAAEASEELMEKYLGG
EELTRAEIVKALRDRTIACEIQPMLCGTAFKNKGVQRMLDAVIDFLPSPVDIPPVTGTDEDDSEKKLERKADDTEKFSAL
AFKIMTDPFVGQLIFFRVYSGKINSGDTVYNPVKQKKERLGRILQMHANQREEIKEVLAGDIAAAVGLKDATTGDTLCDP
AAPIVLERMIFPEPVISQAVEPKTKADQEKMGIALNRLAAEDPSFRVRTDEESGQTIISGMGELHLEILVDRMKREFGVE
ANIGAPQVAYRETIRKTAEGVEGKFVKQSGGRGQYGHAVITLEPQEPGKGFEFIDAIKGGVIPREYIPAVEKGIVDTLPS
GILAGFPVVDVKVTLTFGSYHDVDSNENAFRMAGSMAFKEAMRKASPVLLEPMMAVEVETPEDYTGTVMGDLSSRRGIVQ
GMDDMVGGGKIIKAEVPLSEMFGYSTSLRSATQGRATYTMEFKHYAEAPKNIAEAVMAAKGK

Sequences:

>Translated_702_residues
MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTAFWKGMGGNYP
EHRFNIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYDQLKT
RLKANPVPVVVPIGAEDGFQGVVDLLEMKAIIWDEASQGVKFEYQDIPAELQATADEWREKMVESAAEASEELMEKYLGG
EELTRAEIVKALRDRTIACEIQPMLCGTAFKNKGVQRMLDAVIDFLPSPVDIPPVTGTDEDDSEKKLERKADDTEKFSAL
AFKIMTDPFVGQLIFFRVYSGKINSGDTVYNPVKQKKERLGRILQMHANQREEIKEVLAGDIAAAVGLKDATTGDTLCDP
AAPIVLERMIFPEPVISQAVEPKTKADQEKMGIALNRLAAEDPSFRVRTDEESGQTIISGMGELHLEILVDRMKREFGVE
ANIGAPQVAYRETIRKTAEGVEGKFVKQSGGRGQYGHAVITLEPQEPGKGFEFIDAIKGGVIPREYIPAVEKGIVDTLPS
GILAGFPVVDVKVTLTFGSYHDVDSNENAFRMAGSMAFKEAMRKASPVLLEPMMAVEVETPEDYTGTVMGDLSSRRGIVQ
GMDDMVGGGKIIKAEVPLSEMFGYSTSLRSATQGRATYTMEFKHYAEAPKNIAEAVMAAKGK
>Mature_701_residues
ARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTAFWKGMGGNYPE
HRFNIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYDQLKTR
LKANPVPVVVPIGAEDGFQGVVDLLEMKAIIWDEASQGVKFEYQDIPAELQATADEWREKMVESAAEASEELMEKYLGGE
ELTRAEIVKALRDRTIACEIQPMLCGTAFKNKGVQRMLDAVIDFLPSPVDIPPVTGTDEDDSEKKLERKADDTEKFSALA
FKIMTDPFVGQLIFFRVYSGKINSGDTVYNPVKQKKERLGRILQMHANQREEIKEVLAGDIAAAVGLKDATTGDTLCDPA
APIVLERMIFPEPVISQAVEPKTKADQEKMGIALNRLAAEDPSFRVRTDEESGQTIISGMGELHLEILVDRMKREFGVEA
NIGAPQVAYRETIRKTAEGVEGKFVKQSGGRGQYGHAVITLEPQEPGKGFEFIDAIKGGVIPREYIPAVEKGIVDTLPSG
ILAGFPVVDVKVTLTFGSYHDVDSNENAFRMAGSMAFKEAMRKASPVLLEPMMAVEVETPEDYTGTVMGDLSSRRGIVQG
MDDMVGGGKIIKAEVPLSEMFGYSTSLRSATQGRATYTMEFKHYAEAPKNIAEAVMAAKGK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=703, Percent_Identity=44.6657183499289, Blast_Score=570, Evalue=1e-162,
Organism=Homo sapiens, GI19923640, Length=719, Percent_Identity=39.0820584144645, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI25306287, Length=719, Percent_Identity=36.8567454798331, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI25306283, Length=457, Percent_Identity=40.7002188183807, Blast_Score=306, Evalue=5e-83,
Organism=Homo sapiens, GI157426893, Length=154, Percent_Identity=34.4155844155844, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=39.7058823529412, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=38.3561643835616, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI310132016, Length=120, Percent_Identity=40, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI310110807, Length=120, Percent_Identity=40, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI310123363, Length=120, Percent_Identity=40, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1789738, Length=705, Percent_Identity=74.468085106383, Blast_Score=1073, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=495, Percent_Identity=27.2727272727273, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI48994988, Length=182, Percent_Identity=36.8131868131868, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788922, Length=184, Percent_Identity=33.695652173913, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=691, Percent_Identity=41.8234442836469, Blast_Score=510, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17556745, Length=722, Percent_Identity=30.4709141274238, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17506493, Length=425, Percent_Identity=28.7058823529412, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=163, Percent_Identity=38.6503067484663, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=146, Percent_Identity=31.5068493150685, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=146, Percent_Identity=31.5068493150685, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=698, Percent_Identity=42.6934097421203, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322359, Length=798, Percent_Identity=31.328320802005, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324707, Length=496, Percent_Identity=27.2177419354839, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320593, Length=496, Percent_Identity=27.2177419354839, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323320, Length=191, Percent_Identity=34.0314136125654, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=37.5, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24582462, Length=704, Percent_Identity=43.1818181818182, Blast_Score=568, Evalue=1e-162,
Organism=Drosophila melanogaster, GI221458488, Length=746, Percent_Identity=32.9758713136729, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24585711, Length=423, Percent_Identity=26.4775413711584, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585713, Length=423, Percent_Identity=26.4775413711584, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585709, Length=423, Percent_Identity=26.4775413711584, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI78706572, Length=182, Percent_Identity=36.2637362637363, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574573, Length=143, Percent_Identity=37.7622377622378, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77371; Mature: 77240

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERG
CCCCCHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTAFWKGMGGNYPEHRFNIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ
EEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECHHHHHHHHHHEEHHECCCCC
PQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYDQLKTRLKANPVPVVVPIGAEDGFQ
CCHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHH
GVVDLLEMKAIIWDEASQGVKFEYQDIPAELQATADEWREKMVESAAEASEELMEKYLGG
HHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EELTRAEIVKALRDRTIACEIQPMLCGTAFKNKGVQRMLDAVIDFLPSPVDIPPVTGTDE
HHHHHHHHHHHHHCCEEEEEEEHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DDSEKKLERKADDTEKFSALAFKIMTDPFVGQLIFFRVYSGKINSGDTVYNPVKQKKERL
CCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHH
GRILQMHANQREEIKEVLAGDIAAAVGLKDATTGDTLCDPAAPIVLERMIFPEPVISQAV
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHC
EPKTKADQEKMGIALNRLAAEDPSFRVRTDEESGQTIISGMGELHLEILVDRMKREFGVE
CCCHHCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ANIGAPQVAYRETIRKTAEGVEGKFVKQSGGRGQYGHAVITLEPQEPGKGFEFIDAIKGG
CCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCC
VIPREYIPAVEKGIVDTLPSGILAGFPVVDVKVTLTFGSYHDVDSNENAFRMAGSMAFKE
CCCHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AMRKASPVLLEPMMAVEVETPEDYTGTVMGDLSSRRGIVQGMDDMVGGGKIIKAEVPLSE
HHHHCCCCCCCCEEEEEECCCCCCCCCEECCCHHCCCHHHCHHHHCCCCEEEEECCCHHH
MFGYSTSLRSATQGRATYTMEFKHYAEAPKNIAEAVMAAKGK
HHCCCHHHHHHCCCCEEEEEEHHHHHHCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERG
CCCCHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTAFWKGMGGNYPEHRFNIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ
EEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEECHHHHHHHHHHEEHHECCCCC
PQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYDQLKTRLKANPVPVVVPIGAEDGFQ
CCHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHH
GVVDLLEMKAIIWDEASQGVKFEYQDIPAELQATADEWREKMVESAAEASEELMEKYLGG
HHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EELTRAEIVKALRDRTIACEIQPMLCGTAFKNKGVQRMLDAVIDFLPSPVDIPPVTGTDE
HHHHHHHHHHHHHCCEEEEEEEHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DDSEKKLERKADDTEKFSALAFKIMTDPFVGQLIFFRVYSGKINSGDTVYNPVKQKKERL
CCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHH
GRILQMHANQREEIKEVLAGDIAAAVGLKDATTGDTLCDPAAPIVLERMIFPEPVISQAV
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHC
EPKTKADQEKMGIALNRLAAEDPSFRVRTDEESGQTIISGMGELHLEILVDRMKREFGVE
CCCHHCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ANIGAPQVAYRETIRKTAEGVEGKFVKQSGGRGQYGHAVITLEPQEPGKGFEFIDAIKGG
CCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCC
VIPREYIPAVEKGIVDTLPSGILAGFPVVDVKVTLTFGSYHDVDSNENAFRMAGSMAFKE
CCCHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AMRKASPVLLEPMMAVEVETPEDYTGTVMGDLSSRRGIVQGMDDMVGGGKIIKAEVPLSE
HHHHCCCCCCCCEEEEEECCCCCCCCCEECCCHHCCCHHHCHHHHCCCCEEEEECCCHHH
MFGYSTSLRSATQGRATYTMEFKHYAEAPKNIAEAVMAAKGK
HHCCCHHHHHHCCCCEEEEEEHHHHHHCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA