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The map label for this gene is tuf [H]

Identifier: 73542866

GI number: 73542866

Start: 3481476

End: 3482666

Strand: Reverse

Name: tuf [H]

Synonym: Reut_A3182

Alternate gene names: 73542866

Gene position: 3482666-3481476 (Counterclockwise)

Preceding gene: 73542867

Following gene: 73542865

Centisome position: 91.49

GC content: 62.05

Gene sequence:

>1191_bases
ATGGCAAAGGAAAAGTTCGAGCGGACTAAGCCGCACGTGAACGTTGGTACGATTGGTCACGTTGACCATGGCAAGACCAC
GCTGACCGCAGCGATCGCCACGGTGCTGGCAGCGAAGTTCGGCGGTGCCGCCAAGAAGTACGACGAAATCGACGCAGCGC
CGGAAGAGAAGGCACGCGGTATTACCATCAATACCGCCCACGTCGAATACGAAACGGCCAACCGCCACTACGCGCACGTT
GACTGCCCGGGCCACGCCGACTACGTGAAGAACATGATCACGGGTGCCGCCCAGATGGACGGCGCGATCCTGGTTTGCTC
GGCCGCTGACGGCCCGATGCCGCAAACGCGTGAGCACATCCTGCTGGCCCGCCAGGTTGGCGTGCCGTACATCATCGTGT
TCCTGAACAAGTGCGACATGGTGGACGACGCTGAACTGCTCGAGCTGGTCGAGATGGAAGTTCGCGAACTGCTGTCGAAG
TACGAATTCCCCGGCGACGACACCCCGATCATCAAGGGTTCGGCCAAGCTGGCGCTGGAAGGCGACAAGGGCGAGCTCGG
CGAAGTGGCCATCATGAACCTGGCCGACGCGCTGGACACCTACATCCCGACGCCGGAGCGTGCCGTTGACGGTACCTTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCGGGTCGTGGCACCGTGGTGACCGGCCGTATCGAGCGCGGCGTGATCAAG
GTCGGCGAAGAAATCGAAATCGTCGGTATCCGCCCGACCGTGAAGACCACCTGCACCGGCGTGGAAATGTTCCGCAAGCT
GCTGGACCAGGGCCAAGCTGGCGACAACGTCGGCCTGCTGCTGCGTGGTACCAAGCGTGAAGACGTCGAGCGCGGCCAAG
TGCTGTGCAAGCCGGGTTCGATCAAGCCGCACACCCACTTCACCGGTGAGGTGTACATCCTGTCGAAGGACGAAGGCGGC
CGTCACACCCCGTTCTTCAACAACTACCGCCCGCAGTTCTACTTCCGTACCACCGACGTGACCGGCTCGATCGAGCTGCC
GGCGGACAAGGAAATGGTGATGCCGGGTGATAACGTGTCGATCACCGTCAAGCTGATCGCCCCGATCGCCATGGAAGAAG
GTCTGCGCTTCGCTATCCGTGAAGGCGGCCGTACCGTCGGCGCCGGCGTCGTGGCCAAGATCCTCGACTAA

Upstream 100 bases:

>100_bases
GATGGCGGCCAAGGGCAAGTAATTGGAAGTTTGCCGCCGCGCCAGCCGGTGCGGCGGCGCGATGAAACAACAAGACTGCA
TTCCTATCAGGAGCTAAGAA

Downstream 100 bases:

>100_bases
GTAACTTCCTGGGGCATGCCTTCAGGCATGTCCCCTTTGATCCGGGCACATTGCCCATCGCTCTTTTAAGGAAATATCAT
GCAGAACCAGAAGATCCGTA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLAAKFGGAAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YEFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVIK
VGEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKILD

Sequences:

>Translated_396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLAAKFGGAAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YEFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVIK
VGEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKILD
>Mature_395_residues
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLAAKFGGAAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKY
EFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVIKV
GEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGGR
HTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKILD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.1218274111675, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=30.8045977011494, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=30.8045977011494, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.125, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.3482142857143, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI46094014, Length=439, Percent_Identity=27.5626423690205, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=28.3410138248848, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI194018520, Length=324, Percent_Identity=30.8641975308642, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI194097354, Length=324, Percent_Identity=30.8641975308642, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=27.9874213836478, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=28.6245353159851, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=31.8518518518519, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI53729339, Length=230, Percent_Identity=31.7391304347826, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI53729337, Length=230, Percent_Identity=31.7391304347826, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=80.5063291139241, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=80.5063291139241, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=341, Percent_Identity=30.2052785923754, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI48994988, Length=239, Percent_Identity=31.3807531380753, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789108, Length=324, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788922, Length=331, Percent_Identity=25.6797583081571, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.8535980148883, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=389, Percent_Identity=45.5012853470437, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=457, Percent_Identity=30.1969365426696, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=457, Percent_Identity=30.1969365426696, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=28.310502283105, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=297, Percent_Identity=31.3131313131313, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=26.8518518518519, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=26.8518518518519, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=353, Percent_Identity=28.328611898017, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=37.012987012987, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=305, Percent_Identity=25.5737704918033, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.4261603375527, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.6565656565657, Blast_Score=531, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=30.3370786516854, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=30.3370786516854, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=29.4642857142857, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322937, Length=338, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=274, Percent_Identity=27.7372262773723, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=197, Percent_Identity=30.9644670050761, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=28.1512605042017, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.2875318066158, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.2875318066158, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=51.8041237113402, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=28.9473684210526, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=28.9473684210526, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=28.7912087912088, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=28.7912087912088, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=28.7912087912088, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=28.7912087912088, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI17137380, Length=346, Percent_Identity=30.9248554913295, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45550900, Length=349, Percent_Identity=28.3667621776504, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=29.9674267100977, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647046, Length=278, Percent_Identity=28.4172661870504, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=278, Percent_Identity=28.4172661870504, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572034, Length=232, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43032; Mature: 42901

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLAAKFGGAAKKYDEIDAAPEEKARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYEFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVIK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEEEEECCCEEE
VGEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVS
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKILD
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLAAKFGGAAKKYDEIDAAPEEKARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYEFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVIK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEEEEECCCEEE
VGEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVS
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKILD
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA