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The map label for this gene is pilQ [H]

Identifier: 73542815

GI number: 73542815

Start: 3441034

End: 3443154

Strand: Reverse

Name: pilQ [H]

Synonym: Reut_A3131

Alternate gene names: 73542815

Gene position: 3443154-3441034 (Counterclockwise)

Preceding gene: 73542816

Following gene: 73542814

Centisome position: 90.45

GC content: 64.21

Gene sequence:

>2121_bases
ATGACCCTGGGTCGCTGGTACCGAGCGCTTGCAGGCGCAATGACGATGACACTTGTGCTGGCCGCCCCGGCGGCGCTCGC
ACAGGGCAACCAGGTGAAGTCGGTCGACGCCAATGTGGTGGGCGAGCAGACCCTGTTCACCATCGACCTGCAGTCGCCGC
CGGCGCAGAAGCCGGCGGACTTCACAACGCAGAATCCGGCTCGCATCGCGATCGATTTCTTCGATACCGGGTTTGCCGCC
GGCCGCGCTCAGTATGACTACGCCGGCAAGCTGCTGCGCGGGGCGAGTGTCATGCAGATCGGTGACCGCACCCGCGTGGT
GCTCGACCTGACGCGCACGGCGACTTACCGGACCGAGGTCCGCGGCAACCAGTTCCTGGTGCTGCTCGACAATGTCGCGC
CTGCTGCCAGGGCGGCCGCGCCGACCTTTGCCGCGCCTGCCGCACCGACCGCGGTCGCCGCGGCACGTGCGTCCGTGCGC
AACATCGACTTCCGGCGGGGCACGGATGGCGCGGGACGCGTGGTGGTGGACCTGTCCTCGCGTGATTCGGGCATCAATAT
CGCGCAGCAAGGCCAGAACGTGGTGGTCGATTTCCTGGGCACCTCGCTGCCCGAGAACCTGCGCCGTCGTTTCGACGTGA
GCGACTTCGGCACGCCGGTGCAGGGCATGCGCGCCACGGACGCCAGCGGCAATACCCGCCTGACCATCGAGCCGCGCGGC
AACTGGGAGTACAGCTCCTACCAGACCGACACGCAGTTCGTGGTGGAAGTACGGCCGATCAAGGAAGACCCGGCCAAGCT
GATCAGCGGCCCGGGTTACCGTGGCGAGCGCCTGTCGCTCAACTTCCAGAACATCGACATCCGCTCGCTGCTGCAGGTGT
TTGCCGATTTCACGAACCTGAACATCATTACGAGCGACACCGTACAGGGCAATATCACGCTGCGTCTCAAGGACGTGCCG
TGGGACCAGGCGCTGCAGATCGTGATGGATGCCAAGGGGCTGGCGTCGCGACGCAATGGCAATGTGCTGTGGGTGGCGCC
CAAGGCTGAACTGGCCACCAAGGAGAAGCTGGAGTTGGAGGCGCAGCAGCAGATCAATGAACTCGAGCCGATCCGCAGCC
AGGTGTTCCAGCTGAACTACCAGCGCGCCGAGGATGTGCGCAAGATGCTGCTCGGCATCACCACGGGCGGTGCCGCAGGC
GCGGCGGTCCCGGCTGTCGGCATCGGCGGCGCGGCCGGCGTGGCTTCGCGCATGCTGTCCAAGCGCGGTTCGCTGACTGC
CGATGCCCGCACCAACCAGCTGTTCGTGTCGGACATCGCCAGCAAGCTGGAAGAGGTGCAGGGGTTCCTGAACAAGATCG
ACATCCCGGTGCGCCAGGTGATCATCGAGGCGCGCATCGTCGAGGCCACCGACACGTTCAGCCGTAACCTCGGCGTAAAG
CTCGGCTTTGCCGGCCAGTACAACAACGCCCGGGTCGGCAACACGTACAGCAACGTGCTGCCTGGCGCGAATTCCGATAA
CGGGCCGTTCCTGAGCCTGCCGGCCGGAGCGATCAACGGTACGAACCCGGCGAACATCGCGGTCAGCATCTTCAATAGCG
CCGCCACCCGGTTCCTGGCGCTCGAACTCTCGGCGCTGGAAGCGGATGGCAAGGGCAAGATCATTTCCAGCCCGCGCGTG
GTCACAGCTAACAACATCAAGGCGCTGATCGAGCAAGGTACGGAACTGCCTTACCAGGCAGCCACGTCCAGTGGCGCCAC
CTCGGTGCAGTTCCGCAAGGCCAACCTGAAGCTGGAAGTGACGCCCCAGATCACGCCGGAAGGCAATGTGCTGCTCGACG
TGGATGTGAACAAGGACAGCGTGGGCATCCAGACGACCTCGGGCTTCGCCATCGATACCAAGCACGTGCAGACCCAGGTG
CTGGTAGACAATGGCGGCACAGTGGTCATCGGCGGTATCTATACGCAAACGGAAAGCACGGATGTGGACAAGGTGCCGTT
CCTGGGCGATATCCCTGTGCTCGGCTATCTGTTCAAGAACAACGCGAAGGTGCGCAACCGCACCGAACTGCTGGTGTTCC
TGACGCCGCGTGTCCTGAACGAGAGCGTTACGCTGAAATGA

Upstream 100 bases:

>100_bases
TCCGCATCACCGATCAGGAGATCGTGTTGCGCGAATTGGTCCGGGAAGGGGTATCCGAGTGGAAAGAGAAAATGACCAGC
CTGAAGCTGGAGGTGTCGGC

Downstream 100 bases:

>100_bases
CGCGCCCGGCGGCCCGGAGAGGCGCAATTGGCGCCCGGAGGGCTGGATGCAGTAGACTGCAAGGCCGCATCGACCGATGC
GGCCTTTTTTTGTCCGTGGC

Product: type II and III secretion system secretin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MTLGRWYRALAGAMTMTLVLAAPAALAQGNQVKSVDANVVGEQTLFTIDLQSPPAQKPADFTTQNPARIAIDFFDTGFAA
GRAQYDYAGKLLRGASVMQIGDRTRVVLDLTRTATYRTEVRGNQFLVLLDNVAPAARAAAPTFAAPAAPTAVAAARASVR
NIDFRRGTDGAGRVVVDLSSRDSGINIAQQGQNVVVDFLGTSLPENLRRRFDVSDFGTPVQGMRATDASGNTRLTIEPRG
NWEYSSYQTDTQFVVEVRPIKEDPAKLISGPGYRGERLSLNFQNIDIRSLLQVFADFTNLNIITSDTVQGNITLRLKDVP
WDQALQIVMDAKGLASRRNGNVLWVAPKAELATKEKLELEAQQQINELEPIRSQVFQLNYQRAEDVRKMLLGITTGGAAG
AAVPAVGIGGAAGVASRMLSKRGSLTADARTNQLFVSDIASKLEEVQGFLNKIDIPVRQVIIEARIVEATDTFSRNLGVK
LGFAGQYNNARVGNTYSNVLPGANSDNGPFLSLPAGAINGTNPANIAVSIFNSAATRFLALELSALEADGKGKIISSPRV
VTANNIKALIEQGTELPYQAATSSGATSVQFRKANLKLEVTPQITPEGNVLLDVDVNKDSVGIQTTSGFAIDTKHVQTQV
LVDNGGTVVIGGIYTQTESTDVDKVPFLGDIPVLGYLFKNNAKVRNRTELLVFLTPRVLNESVTLK

Sequences:

>Translated_706_residues
MTLGRWYRALAGAMTMTLVLAAPAALAQGNQVKSVDANVVGEQTLFTIDLQSPPAQKPADFTTQNPARIAIDFFDTGFAA
GRAQYDYAGKLLRGASVMQIGDRTRVVLDLTRTATYRTEVRGNQFLVLLDNVAPAARAAAPTFAAPAAPTAVAAARASVR
NIDFRRGTDGAGRVVVDLSSRDSGINIAQQGQNVVVDFLGTSLPENLRRRFDVSDFGTPVQGMRATDASGNTRLTIEPRG
NWEYSSYQTDTQFVVEVRPIKEDPAKLISGPGYRGERLSLNFQNIDIRSLLQVFADFTNLNIITSDTVQGNITLRLKDVP
WDQALQIVMDAKGLASRRNGNVLWVAPKAELATKEKLELEAQQQINELEPIRSQVFQLNYQRAEDVRKMLLGITTGGAAG
AAVPAVGIGGAAGVASRMLSKRGSLTADARTNQLFVSDIASKLEEVQGFLNKIDIPVRQVIIEARIVEATDTFSRNLGVK
LGFAGQYNNARVGNTYSNVLPGANSDNGPFLSLPAGAINGTNPANIAVSIFNSAATRFLALELSALEADGKGKIISSPRV
VTANNIKALIEQGTELPYQAATSSGATSVQFRKANLKLEVTPQITPEGNVLLDVDVNKDSVGIQTTSGFAIDTKHVQTQV
LVDNGGTVVIGGIYTQTESTDVDKVPFLGDIPVLGYLFKNNAKVRNRTELLVFLTPRVLNESVTLK
>Mature_705_residues
TLGRWYRALAGAMTMTLVLAAPAALAQGNQVKSVDANVVGEQTLFTIDLQSPPAQKPADFTTQNPARIAIDFFDTGFAAG
RAQYDYAGKLLRGASVMQIGDRTRVVLDLTRTATYRTEVRGNQFLVLLDNVAPAARAAAPTFAAPAAPTAVAAARASVRN
IDFRRGTDGAGRVVVDLSSRDSGINIAQQGQNVVVDFLGTSLPENLRRRFDVSDFGTPVQGMRATDASGNTRLTIEPRGN
WEYSSYQTDTQFVVEVRPIKEDPAKLISGPGYRGERLSLNFQNIDIRSLLQVFADFTNLNIITSDTVQGNITLRLKDVPW
DQALQIVMDAKGLASRRNGNVLWVAPKAELATKEKLELEAQQQINELEPIRSQVFQLNYQRAEDVRKMLLGITTGGAAGA
AVPAVGIGGAAGVASRMLSKRGSLTADARTNQLFVSDIASKLEEVQGFLNKIDIPVRQVIIEARIVEATDTFSRNLGVKL
GFAGQYNNARVGNTYSNVLPGANSDNGPFLSLPAGAINGTNPANIAVSIFNSAATRFLALELSALEADGKGKIISSPRVV
TANNIKALIEQGTELPYQAATSSGATSVQFRKANLKLEVTPQITPEGNVLLDVDVNKDSVGIQTTSGFAIDTKHVQTQVL
VDNGGTVVIGGIYTQTESTDVDKVPFLGDIPVLGYLFKNNAKVRNRTELLVFLTPRVLNESVTLK

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=444, Percent_Identity=33.3333333333333, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87082242, Length=389, Percent_Identity=30.0771208226221, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 75806; Mature: 75675

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLGRWYRALAGAMTMTLVLAAPAALAQGNQVKSVDANVVGEQTLFTIDLQSPPAQKPAD
CCHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCC
FTTQNPARIAIDFFDTGFAAGRAQYDYAGKLLRGASVMQIGDRTRVVLDLTRTATYRTEV
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEEEEEECCEEEEEEC
RGNQFLVLLDNVAPAARAAAPTFAAPAAPTAVAAARASVRNIDFRRGTDGAGRVVVDLSS
CCCEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
RDSGINIAQQGQNVVVDFLGTSLPENLRRRFDVSDFGTPVQGMRATDASGNTRLTIEPRG
CCCCCCHHHCCCEEEEEECCCCCCHHHHHHCCCHHCCCCCCCCEEECCCCCEEEEEEECC
NWEYSSYQTDTQFVVEVRPIKEDPAKLISGPGYRGERLSLNFQNIDIRSLLQVFADFTNL
CCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCC
NIITSDTVQGNITLRLKDVPWDQALQIVMDAKGLASRRNGNVLWVAPKAELATKEKLELE
EEEEECCEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHCCHHHHHHH
AQQQINELEPIRSQVFQLNYQRAEDVRKMLLGITTGGAAGAAVPAVGIGGAAGVASRMLS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHH
KRGSLTADARTNQLFVSDIASKLEEVQGFLNKIDIPVRQVIIEARIVEATDTFSRNLGVK
HCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEECHHHHHCCCCEE
LGFAGQYNNARVGNTYSNVLPGANSDNGPFLSLPAGAINGTNPANIAVSIFNSAATRFLA
EEECCCCCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEHHHHHEEEE
LELSALEADGKGKIISSPRVVTANNIKALIEQGTELPYQAATSSGATSVQFRKANLKLEV
EEEEEECCCCCCCEECCCEEEECCHHHHHHHCCCCCCCHHCCCCCCCEEEEEECCEEEEE
TPQITPEGNVLLDVDVNKDSVGIQTTSGFAIDTKHVQTQVLVDNGGTVVIGGIYTQTEST
CCEECCCCCEEEEEECCCCCCCEEECCCEEEECCCEEEEEEEECCCEEEEEEEEECCCCC
DVDKVPFLGDIPVLGYLFKNNAKVRNRTELLVFLTPRVLNESVTLK
CCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECHHHHCCCCCCC
>Mature Secondary Structure 
TLGRWYRALAGAMTMTLVLAAPAALAQGNQVKSVDANVVGEQTLFTIDLQSPPAQKPAD
CHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCC
FTTQNPARIAIDFFDTGFAAGRAQYDYAGKLLRGASVMQIGDRTRVVLDLTRTATYRTEV
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEEEEEECCEEEEEEC
RGNQFLVLLDNVAPAARAAAPTFAAPAAPTAVAAARASVRNIDFRRGTDGAGRVVVDLSS
CCCEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
RDSGINIAQQGQNVVVDFLGTSLPENLRRRFDVSDFGTPVQGMRATDASGNTRLTIEPRG
CCCCCCHHHCCCEEEEEECCCCCCHHHHHHCCCHHCCCCCCCCEEECCCCCEEEEEEECC
NWEYSSYQTDTQFVVEVRPIKEDPAKLISGPGYRGERLSLNFQNIDIRSLLQVFADFTNL
CCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCC
NIITSDTVQGNITLRLKDVPWDQALQIVMDAKGLASRRNGNVLWVAPKAELATKEKLELE
EEEEECCEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHCCHHHHHHH
AQQQINELEPIRSQVFQLNYQRAEDVRKMLLGITTGGAAGAAVPAVGIGGAAGVASRMLS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHH
KRGSLTADARTNQLFVSDIASKLEEVQGFLNKIDIPVRQVIIEARIVEATDTFSRNLGVK
HCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEECHHHHHCCCCEE
LGFAGQYNNARVGNTYSNVLPGANSDNGPFLSLPAGAINGTNPANIAVSIFNSAATRFLA
EEECCCCCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEHHHHHEEEE
LELSALEADGKGKIISSPRVVTANNIKALIEQGTELPYQAATSSGATSVQFRKANLKLEV
EEEEEECCCCCCCEECCCEEEECCHHHHHHHCCCCCCCHHCCCCCCCEEEEEECCEEEEE
TPQITPEGNVLLDVDVNKDSVGIQTTSGFAIDTKHVQTQVLVDNGGTVVIGGIYTQTEST
CCEECCCCCEEEEEECCCCCCCEEECCCEEEECCCEEEEEEEECCCEEEEEEEEECCCCC
DVDKVPFLGDIPVLGYLFKNNAKVRNRTELLVFLTPRVLNESVTLK
CCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]