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The map label for this gene is aroK

Identifier: 73542814

GI number: 73542814

Start: 3440214

End: 3440792

Strand: Reverse

Name: aroK

Synonym: Reut_A3130

Alternate gene names: 73542814

Gene position: 3440792-3440214 (Counterclockwise)

Preceding gene: 73542815

Following gene: 73542813

Centisome position: 90.39

GC content: 65.46

Gene sequence:

>579_bases
ATGGAGACCGGCCGTCCCAACCTCTTCTTCGTCGGCTTGATGGGCGCCGGCAAGACTACCGTGGGCCGGACGGTCGCGCG
GCGCCTGCACTATCCGTTTTTTGATTCCGACCACGAGCTGGAAGCGCACTGCGGCGTGCGCATCCCGGTGATTTTCGAGC
ACGAGGGCGAAGAAGGTTTCCGCGACCGCGAGGCCGCCATGATCCGTGAACTCACCGGCCGCAGCGGCATTGTGCTTGCC
ACAGGCGGCGGCGCGGTGCTGCGGCCCGAAAACCGCGAACTGCTCAAATCACGCGGCACTGTGGTCTACCTGCGCGCCAG
CCCGCACGACCTGTGGCTGCGCACCCGTCACGACCGCAACCGCCCGCTGCTGCAGACCGAGGATCCGAAGGGCAAGCTCG
AAGCGCTCTATGGCGCGCGCGACCCGCTTTACCGCGAGGTGGCCGATTTCATCATCGAGACCGGCAAGCCCTCGGTGGCA
CAGCTTGCTAATATGGTGCTGATGCAGCTGGAAATGGCCGGTTTCCGCATCGACACCGCCGCCGCGTCGGACCAGCCGGC
CAACGATACGCATTCATGA

Upstream 100 bases:

>100_bases
TGCGGCCCAACATAAGAAGATGATGCAGTTTCCAACGGGGGAGGGCGGACCGGAATCCGCATTCCCGAGCGACAATCCAG
TGACAGCCTTGCCGATGGCC

Downstream 100 bases:

>100_bases
TCACGCTACAAGTCGACCTGGGAGAGCGCAGCTATCCCATCCATATCGGTACCGGCCTGCTCGGCAACGCCGAGCTGCTG
CGCCCGCATGTGCGCGGCCA

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
METGRPNLFFVGLMGAGKTTVGRTVARRLHYPFFDSDHELEAHCGVRIPVIFEHEGEEGFRDREAAMIRELTGRSGIVLA
TGGGAVLRPENRELLKSRGTVVYLRASPHDLWLRTRHDRNRPLLQTEDPKGKLEALYGARDPLYREVADFIIETGKPSVA
QLANMVLMQLEMAGFRIDTAAASDQPANDTHS

Sequences:

>Translated_192_residues
METGRPNLFFVGLMGAGKTTVGRTVARRLHYPFFDSDHELEAHCGVRIPVIFEHEGEEGFRDREAAMIRELTGRSGIVLA
TGGGAVLRPENRELLKSRGTVVYLRASPHDLWLRTRHDRNRPLLQTEDPKGKLEALYGARDPLYREVADFIIETGKPSVA
QLANMVLMQLEMAGFRIDTAAASDQPANDTHS
>Mature_192_residues
METGRPNLFFVGLMGAGKTTVGRTVARRLHYPFFDSDHELEAHCGVRIPVIFEHEGEEGFRDREAAMIRELTGRSGIVLA
TGGGAVLRPENRELLKSRGTVVYLRASPHDLWLRTRHDRNRPLLQTEDPKGKLEALYGARDPLYREVADFIIETGKPSVA
QLANMVLMQLEMAGFRIDTAAASDQPANDTHS

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=166, Percent_Identity=49.3975903614458, Blast_Score=160, Evalue=4e-41,
Organism=Escherichia coli, GI1786587, Length=155, Percent_Identity=38.7096774193548, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_CUPPJ (Q46WJ3)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_297334.1
- ProteinModelPortal:   Q46WJ3
- SMR:   Q46WJ3
- GeneID:   3611956
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A3130
- NMPDR:   fig|264198.3.peg.3566
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- ProtClustDB:   PRK00131
- BioCyc:   REUT264198:REUT_A3130-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 21410; Mature: 21410

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01128 SHIKIMATE_KINASE; PS00267 TACHYKININ

Important sites: BINDING 37-37 BINDING 61-61 BINDING 83-83 BINDING 121-121 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGRPNLFFVGLMGAGKTTVGRTVARRLHYPFFDSDHELEAHCGVRIPVIFEHEGEEGF
CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCC
RDREAAMIRELTGRSGIVLATGGGAVLRPENRELLKSRGTVVYLRASPHDLWLRTRHDRN
HHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHCCCCEEEEEECCCEEEEEECCCCC
RPLLQTEDPKGKLEALYGARDPLYREVADFIIETGKPSVAQLANMVLMQLEMAGFRIDTA
CCCEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEECC
AASDQPANDTHS
CCCCCCCCCCCC
>Mature Secondary Structure
METGRPNLFFVGLMGAGKTTVGRTVARRLHYPFFDSDHELEAHCGVRIPVIFEHEGEEGF
CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCC
RDREAAMIRELTGRSGIVLATGGGAVLRPENRELLKSRGTVVYLRASPHDLWLRTRHDRN
HHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHCCCCEEEEEECCCEEEEEECCCCC
RPLLQTEDPKGKLEALYGARDPLYREVADFIIETGKPSVAQLANMVLMQLEMAGFRIDTA
CCCEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEECC
AASDQPANDTHS
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA