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The map label for this gene is mlaA [H]

Identifier: 73542803

GI number: 73542803

Start: 3425258

End: 3426169

Strand: Reverse

Name: mlaA [H]

Synonym: Reut_A3119

Alternate gene names: 73542803

Gene position: 3426169-3425258 (Counterclockwise)

Preceding gene: 73542804

Following gene: 73542802

Centisome position: 90.01

GC content: 65.35

Gene sequence:

>912_bases
ATGACCGTTCCTTCGTGCAAGCCGGCGGCCAGGCACGCTGTCCTGGCGTTGGCAGCTGGTGTCCTGATGGGGCTGGCGGG
TTGCGCCACCGGCCCGACCGCCAATCCGAAGGATCCGCTCGAGCCGTTCAATCGCGAGGTCTCGCGCATCAATGAAGACT
TCGACAAGGGCGTGCTGAGGCCTGTTGCCCAGCTTTATGCGGACTACATTCCGTCGCCCGTCCAGACCGCGGTGGATAAC
TTCTTCTCGAATGTCAGCGACGTCTATTCGGCGGTGAACAACCTGCTGCAGGGCAAGCCCAGCCGTGCGGCCGAGGACAC
CATGCGAGTGGCGATCAACACCGTGCTAGGTATTGGCGGCCTGATCGACATTGCCACGCCGGGCGGCCTGCCCAAGTACA
AGGAAGATTTCGGCCAGACGCTTGGTGTCTGGGGCGTGCCGGCGGGGCCGTACCTGGTGCTGCCGCTGTTTGGGCCGAGC
ACCGTGCGCGACACCGCGGGCATGCTGGTGGACCGTCAGATTGACCCTTCGGCCTATTTCTACCCCGTTTCGCTGCGCAA
CTCGTTGGTCGGGGTGCGCATTGTTGCGGGCCGCGCGCAACTGCTTGGCGCGACCAACCTGCTCGAGCAGGCCGCGCTGG
ACAAGTATGCGTTCCTGCGCGATTCCTATCTGCAGCGGCGTGAGTATCTGATCTACGACGGCAATCCTCCGAGTCGGGAC
AACGATGAATCGGAGAGCGATGGTCAGCCGGCGCAGCCTTCCTCATCGCCTTCGCAGCCGTCGTCCGAACAGCCGTCGCA
GCCGGCCGCGCCCGCCGAAGGCGCCACGCAGCCGGAGGGCCAGAACCTGCCTGTGCCGCCGGCTTCACCGATGCAGCGCA
TCGTTCCCGGCGGTGGCCTGCGCATCCGCTGA

Upstream 100 bases:

>100_bases
TGATCGGGCAGTTCCTGTACAACAAGGCTGCCGACGCCGGGGCCGGCGGGGCCAGCAGCGGTGGTGCGGCGAGCGCGCCG
GCTGTGGGCGGAGCCGGCAA

Downstream 100 bases:

>100_bases
GGTATGGGGGGAGCCTGTAAATGCGCTGTAAGCCGGCTGTGAGTGTGCTGTGAGCGCTGAAACAATTTGTTTTGAAGTGG
CGGGGGATGCACGAGGGCCG

Product: VacJ-like lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTVPSCKPAARHAVLALAAGVLMGLAGCATGPTANPKDPLEPFNREVSRINEDFDKGVLRPVAQLYADYIPSPVQTAVDN
FFSNVSDVYSAVNNLLQGKPSRAAEDTMRVAINTVLGIGGLIDIATPGGLPKYKEDFGQTLGVWGVPAGPYLVLPLFGPS
TVRDTAGMLVDRQIDPSAYFYPVSLRNSLVGVRIVAGRAQLLGATNLLEQAALDKYAFLRDSYLQRREYLIYDGNPPSRD
NDESESDGQPAQPSSSPSQPSSEQPSQPAAPAEGATQPEGQNLPVPPASPMQRIVPGGGLRIR

Sequences:

>Translated_303_residues
MTVPSCKPAARHAVLALAAGVLMGLAGCATGPTANPKDPLEPFNREVSRINEDFDKGVLRPVAQLYADYIPSPVQTAVDN
FFSNVSDVYSAVNNLLQGKPSRAAEDTMRVAINTVLGIGGLIDIATPGGLPKYKEDFGQTLGVWGVPAGPYLVLPLFGPS
TVRDTAGMLVDRQIDPSAYFYPVSLRNSLVGVRIVAGRAQLLGATNLLEQAALDKYAFLRDSYLQRREYLIYDGNPPSRD
NDESESDGQPAQPSSSPSQPSSEQPSQPAAPAEGATQPEGQNLPVPPASPMQRIVPGGGLRIR
>Mature_302_residues
TVPSCKPAARHAVLALAAGVLMGLAGCATGPTANPKDPLEPFNREVSRINEDFDKGVLRPVAQLYADYIPSPVQTAVDNF
FSNVSDVYSAVNNLLQGKPSRAAEDTMRVAINTVLGIGGLIDIATPGGLPKYKEDFGQTLGVWGVPAGPYLVLPLFGPST
VRDTAGMLVDRQIDPSAYFYPVSLRNSLVGVRIVAGRAQLLGATNLLEQAALDKYAFLRDSYLQRREYLIYDGNPPSRDN
DESESDGQPAQPSSSPSQPSSEQPSQPAAPAEGATQPEGQNLPVPPASPMQRIVPGGGLRIR

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=238, Percent_Identity=35.2941176470588, Blast_Score=127, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 32181; Mature: 32049

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPSCKPAARHAVLALAAGVLMGLAGCATGPTANPKDPLEPFNREVSRINEDFDKGVLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
PVAQLYADYIPSPVQTAVDNFFSNVSDVYSAVNNLLQGKPSRAAEDTMRVAINTVLGIGG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
LIDIATPGGLPKYKEDFGQTLGVWGVPAGPYLVLPLFGPSTVRDTAGMLVDRQIDPSAYF
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEE
YPVSLRNSLVGVRIVAGRAQLLGATNLLEQAALDKYAFLRDSYLQRREYLIYDGNPPSRD
EEHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCC
NDESESDGQPAQPSSSPSQPSSEQPSQPAAPAEGATQPEGQNLPVPPASPMQRIVPGGGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
RIR
EEC
>Mature Secondary Structure 
TVPSCKPAARHAVLALAAGVLMGLAGCATGPTANPKDPLEPFNREVSRINEDFDKGVLR
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
PVAQLYADYIPSPVQTAVDNFFSNVSDVYSAVNNLLQGKPSRAAEDTMRVAINTVLGIGG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
LIDIATPGGLPKYKEDFGQTLGVWGVPAGPYLVLPLFGPSTVRDTAGMLVDRQIDPSAYF
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEE
YPVSLRNSLVGVRIVAGRAQLLGATNLLEQAALDKYAFLRDSYLQRREYLIYDGNPPSRD
EEHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCC
NDESESDGQPAQPSSSPSQPSSEQPSQPAAPAEGATQPEGQNLPVPPASPMQRIVPGGGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
RIR
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]