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The map label for this gene is oprM [H]

Identifier: 73542745

GI number: 73542745

Start: 3365303

End: 3366751

Strand: Reverse

Name: oprM [H]

Synonym: Reut_A3061

Alternate gene names: 73542745

Gene position: 3366751-3365303 (Counterclockwise)

Preceding gene: 73542746

Following gene: 73542741

Centisome position: 88.45

GC content: 67.84

Gene sequence:

>1449_bases
ATGAGACCGCGTGCCTGGATGCTCGGCGCCTGCGCGGTGGCGGCGCTGGGCGGCTGCGCGATCGGTCCCAACTACCAGCG
GCCCGAAACGCCCGATGTCTCGCAATACCGCATGGACACCGGCCAGCTTGCCATCGCGGCCGACAGCGACTGGTGGAACC
AGTTCAACGATCCGGTGCTCAATGCGCTGATCGACGAGGCGCTCGCAAACAATTACGACGTGCGCATCGCCGCCGCGCGC
ATCGACGAGTTCCGCGGCCAGCTGATGGTCGCGCGTTCGGGCTTTTTCCCGCAGTTGAACCTCGGCGCGGCCGCCACCCG
TCAGCGCGCGGGTACGTTCAATGGCACGCCGTTCGCGCCGTTTGACGGTCCACGCAATTCATACCAGCTGCTCGCTAACG
CGAGCTGGGAACTCGATCTGTGGGGACGCATCCGCCGCCAGGCTGAAGCCGCGGAAGCGAGCCTGTGGAACGCCGAGTAC
GCACGGCGTGGCGTCGTGCTGTCGCTCGCCGCGTCGGTGGTGCAAGGCTATGCCACGCTGCGCGGGCTCGATGCGCAGCT
CGAGGTCGCGAAGCAGACGCTGGAGTCACGCGCGAGCGGGCTTGATATCTTCAGGCAGCGCTACGAAGGCGGCGTGATCT
CCGAGATGGAGCTGGCGCAGGCGCAGAACGACTTCTATGTCGCCGAGGCTTCCATTCCGCAGATCCGCACCGGCATTGCG
CAGACCGAGAATGCGCTGTCGGTGCTGGTCGGGCGCCAGCCGGGGCCCATCGAGCGGGGCAAGGGCATCTACGAGCTGCA
GGCGCCGTCGGTAGGCGCCGACCTGCCCGCGATGCTGCTGTCGCGCCGGCCCGACGTGCTGCAGGCCGAGCAGGTGGCGG
TCGCGGCTAACGCGCAACTGGGCGCGGCGCAGGCGCTCTACCTCCCGGCCGTCAACCTGAGCGGCTTGTTCGGCGCGGTC
GCCGCGTCACCGGCGGGGTTGTGGGACAGCGCATCGCGGGTGTGGGGGTTTGGTGCGGGACTGACCCAGCCGATCTTCCA
GGGTGGTGCCATCCGTGGGCAAGTGCAGACCGCATCGGCGCAGCGTACCGAGGCCTTGCTGGCTTACCAGGGCACCGTGC
TGGCGGCACTGGCCGACGTGAACAGCGCGCTGGCCAATGGTGTCGAGACGCGCAACCGCCTGGGCAGCCTGCGCCAGCAG
GAAAAGAGCCTGACCGTCTATGCCGATCAGGCCTACGCAAGGTATGAGGGCGGTTATTCGAGCTACCTGGAAGTCACCAA
TGCGCGCGAGAAGCTGTTCGACGCGCAGCTGGCGGCCATCCAGGGACAGGTCGATGTGCTAAACGGCACGGCGGCGCTGT
ACAAGTCGCTCGGTGGTGGCTGGCCGTCGGTGCCCGATACAGTGAAGGGCGCTGGCATGCAGGGCGATGCGCCGGTGCTA
GCGCGCTGA

Upstream 100 bases:

>100_bases
TCTGGGGGCTGGAGCGCATGTCCGAAGGCAAGCCCAAGGGCGAGGCCAAGACAGGTGGCGCGTCGCAGCCGCCTACCATT
CCTCCGGCGGGAGAACCATC

Downstream 100 bases:

>100_bases
CCATTCTCCCTCGCGCACGCCCAGCGCGGTGACCAGTCCGTAGGCGGTGATCCCCATCGCCACGCCGAGGAAATGCCCAT
CCAGCCCCATGCCAGCGACA

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MRPRAWMLGACAVAALGGCAIGPNYQRPETPDVSQYRMDTGQLAIAADSDWWNQFNDPVLNALIDEALANNYDVRIAAAR
IDEFRGQLMVARSGFFPQLNLGAAATRQRAGTFNGTPFAPFDGPRNSYQLLANASWELDLWGRIRRQAEAAEASLWNAEY
ARRGVVLSLAASVVQGYATLRGLDAQLEVAKQTLESRASGLDIFRQRYEGGVISEMELAQAQNDFYVAEASIPQIRTGIA
QTENALSVLVGRQPGPIERGKGIYELQAPSVGADLPAMLLSRRPDVLQAEQVAVAANAQLGAAQALYLPAVNLSGLFGAV
AASPAGLWDSASRVWGFGAGLTQPIFQGGAIRGQVQTASAQRTEALLAYQGTVLAALADVNSALANGVETRNRLGSLRQQ
EKSLTVYADQAYARYEGGYSSYLEVTNAREKLFDAQLAAIQGQVDVLNGTAALYKSLGGGWPSVPDTVKGAGMQGDAPVL
AR

Sequences:

>Translated_482_residues
MRPRAWMLGACAVAALGGCAIGPNYQRPETPDVSQYRMDTGQLAIAADSDWWNQFNDPVLNALIDEALANNYDVRIAAAR
IDEFRGQLMVARSGFFPQLNLGAAATRQRAGTFNGTPFAPFDGPRNSYQLLANASWELDLWGRIRRQAEAAEASLWNAEY
ARRGVVLSLAASVVQGYATLRGLDAQLEVAKQTLESRASGLDIFRQRYEGGVISEMELAQAQNDFYVAEASIPQIRTGIA
QTENALSVLVGRQPGPIERGKGIYELQAPSVGADLPAMLLSRRPDVLQAEQVAVAANAQLGAAQALYLPAVNLSGLFGAV
AASPAGLWDSASRVWGFGAGLTQPIFQGGAIRGQVQTASAQRTEALLAYQGTVLAALADVNSALANGVETRNRLGSLRQQ
EKSLTVYADQAYARYEGGYSSYLEVTNAREKLFDAQLAAIQGQVDVLNGTAALYKSLGGGWPSVPDTVKGAGMQGDAPVL
AR
>Mature_482_residues
MRPRAWMLGACAVAALGGCAIGPNYQRPETPDVSQYRMDTGQLAIAADSDWWNQFNDPVLNALIDEALANNYDVRIAAAR
IDEFRGQLMVARSGFFPQLNLGAAATRQRAGTFNGTPFAPFDGPRNSYQLLANASWELDLWGRIRRQAEAAEASLWNAEY
ARRGVVLSLAASVVQGYATLRGLDAQLEVAKQTLESRASGLDIFRQRYEGGVISEMELAQAQNDFYVAEASIPQIRTGIA
QTENALSVLVGRQPGPIERGKGIYELQAPSVGADLPAMLLSRRPDVLQAEQVAVAANAQLGAAQALYLPAVNLSGLFGAV
AASPAGLWDSASRVWGFGAGLTQPIFQGGAIRGQVQTASAQRTEALLAYQGTVLAALADVNSALANGVETRNRLGSLRQQ
EKSLTVYADQAYARYEGGYSSYLEVTNAREKLFDAQLAAIQGQVDVLNGTAALYKSLGGGWPSVPDTVKGAGMQGDAPVL
AR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=28.5407725321888, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI2367348, Length=485, Percent_Identity=27.4226804123711, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51385; Mature: 51385

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRAWMLGACAVAALGGCAIGPNYQRPETPDVSQYRMDTGQLAIAADSDWWNQFNDPVL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCHHHHHCCHHHH
NALIDEALANNYDVRIAAARIDEFRGQLMVARSGFFPQLNLGAAATRQRAGTFNGTPFAP
HHHHHHHHHCCCCEEEEHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCC
FDGPRNSYQLLANASWELDLWGRIRRQAEAAEASLWNAEYARRGVVLSLAASVVQGYATL
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHH
RGLDAQLEVAKQTLESRASGLDIFRQRYEGGVISEMELAQAQNDFYVAEASIPQIRTGIA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHH
QTENALSVLVGRQPGPIERGKGIYELQAPSVGADLPAMLLSRRPDVLQAEQVAVAANAQL
HHCCHHHEEECCCCCCHHHCCCEEEEECCCCCCCHHHHHHHCCCCCHHHHHEEEECCCCC
GAAQALYLPAVNLSGLFGAVAASPAGLWDSASRVWGFGAGLTQPIFQGGAIRGQVQTASA
CCCCEEEECCCCHHHHHHHHHCCCCCCCCCCHHHEECCCCCHHHHHCCCCCCCEEECCHH
QRTEALLAYQGTVLAALADVNSALANGVETRNRLGSLRQQEKSLTVYADQAYARYEGGYS
HHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEECCHHHHHCCCHH
SYLEVTNAREKLFDAQLAAIQGQVDVLNGTAALYKSLGGGWPSVPDTVKGAGMQGDAPVL
HHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCC
AR
CC
>Mature Secondary Structure
MRPRAWMLGACAVAALGGCAIGPNYQRPETPDVSQYRMDTGQLAIAADSDWWNQFNDPVL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCHHHHHCCHHHH
NALIDEALANNYDVRIAAARIDEFRGQLMVARSGFFPQLNLGAAATRQRAGTFNGTPFAP
HHHHHHHHHCCCCEEEEHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCC
FDGPRNSYQLLANASWELDLWGRIRRQAEAAEASLWNAEYARRGVVLSLAASVVQGYATL
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHH
RGLDAQLEVAKQTLESRASGLDIFRQRYEGGVISEMELAQAQNDFYVAEASIPQIRTGIA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHH
QTENALSVLVGRQPGPIERGKGIYELQAPSVGADLPAMLLSRRPDVLQAEQVAVAANAQL
HHCCHHHEEECCCCCCHHHCCCEEEEECCCCCCCHHHHHHHCCCCCHHHHHEEEECCCCC
GAAQALYLPAVNLSGLFGAVAASPAGLWDSASRVWGFGAGLTQPIFQGGAIRGQVQTASA
CCCCEEEECCCCHHHHHHHHHCCCCCCCCCCHHHEECCCCCHHHHHCCCCCCCEEECCHH
QRTEALLAYQGTVLAALADVNSALANGVETRNRLGSLRQQEKSLTVYADQAYARYEGGYS
HHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEECCHHHHHCCCHH
SYLEVTNAREKLFDAQLAAIQGQVDVLNGTAALYKSLGGGWPSVPDTVKGAGMQGDAPVL
HHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]