Click here to switch to the map view.

The map label for this gene is baiB [H]

Identifier: 73542741

GI number: 73542741

Start: 3360620

End: 3362119

Strand: Reverse

Name: baiB [H]

Synonym: Reut_A3057

Alternate gene names: 73542741

Gene position: 3362119-3360620 (Counterclockwise)

Preceding gene: 73542745

Following gene: 73542740

Centisome position: 88.32

GC content: 68.07

Gene sequence:

>1500_bases
ATGGCCACAGACCTCTGGTTCGAAGACATGCACCGCGACGGCGCCGAACTGCTCGACCGCGGCGCCCGCCTTGCCGGGGG
CCTGCGCGCCGCGGGACTGAAAGAAGGCGATGTCGTTGCGGTGCTGCTGCGCAACGATCCGGTCTATGTCGATGTCGTGC
ACGCTTGCCGCATCGGTGGCACCTACTACTGCCCGATCAACTGGCACTTCACCGCCGAGGAAGTGCGCTTCCTGCTGGCC
GACAGCGGCGCCAAAGCGCTGATCGTGCATGCCGGCCTGCTGGCGGCAGTGCGCGAGGCGGTGCCTGCGGGCATCCCCGT
GCTGGTGGTGGCCGATGGCGCCGAAGTGTCCGGCCGCAGCGAGTACGAAGCGTGGCTTGCGCAGCAGGCTCCGTACGATG
GCCCACGTGTATCCCCGCGCGGCCATATGGCCTACACCTCCGGCACCACGGGCCGTCCCAAGGGCGTGCTGCGCGAACCC
GTCCCGCTGGACCAGCTTGACGACCAGATGGCGCGCGCGCGTTCGCTGGTGCGCCAGACCTACGGCATCGTGCCCGGCTG
TCGGGCGCTGATGTCGGCGCCGCTGTACCACAGCGCGCCCGGTTCGTTCATCCAGAACGCGCTGCAGATGGCCGAACGCC
TCGTGCTCACGTCGCGCTTCGATGCCGAGCAGGTGCTGGCGCTGGTCGAAAAGCACCGCATCGACGTGCTTTATCTCGTC
CCGATCATGTACGTGCGCTTGCTCAAGCTGCCGCCCGAGGTGCGCGCGCGCTACGACCTGTCGTCGATCCGCTTCGTTGC
TTCGACCGGCTCGCCGTGCTCGCCCGAGGTCAAGCGCGCGATGCTCGAATGGTTCGGCCCAGTCATCCACGAAACCTACG
CGTCGAGCGAGGCCGGCATGATCACCGTGGCCACGCCTGCCGATGCCGCGGCGCGCCCCGGCACGGCCGGCAAGCCGGTG
GACGACGCGCAGCTTCGCATCCTCGATGAAGACGGCAAGCCGTGCGCAACGGGCGAGATTGGCCTGGTGTACGTGCGCCA
GCCGGCTTACCCCGACTTCACTTACCGCAACAATGAGGCCGCGCGCCGCGCCATCGACCGCGACGGGCTGGTGACGCTTG
GCGACATGGGCTATGTCGATGCCGACGGCTACCTGTTCATCTGCGACCGCGCGTCCGACATGGTGATCTCGGGCGGCGTG
AACATCTACCCGGCCGAGATCGAACATGAACTCGTGCGCTATCCGGGCGTGGCCGACTGCGTCGTGTTCGGCGTGCCCGA
CGACGAGTACGGCGAGCGCTTGCTGGCCATGGTGCAGCCGATGCCCGACGCGGCGATCCGCGAGGAAGACGTGATCGACT
GGCTGCGCCAACGCCTGTCCGGCTTCAAGGTGCCGCGCAACATCGTGATCGAAGCGCAATTGCCGCGCGACGAGACCGGC
AAACTGGCCAAGCGCCGGCTGCGCGACCGGTACTGGGAAGGGCGGCAGCGGCGCGTCTGA

Upstream 100 bases:

>100_bases
GGCGCAACATTGTGATTCCGGTGCGGCGGATTGTCGATGCCAGGCTGCGCGGCTACAGTGGAACGCTGCCAGTTCAGGAC
AAAGACAGGAGACTATCGAT

Downstream 100 bases:

>100_bases
GATACGCACGGAATACCCGGCAAAGATCAAAAGAAAGGCGCCACGAAGGCGCCGCCATCAACAGGAGACACTATGCAAGG
CAGTAAATTCGGCCGTCGGG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MATDLWFEDMHRDGAELLDRGARLAGGLRAAGLKEGDVVAVLLRNDPVYVDVVHACRIGGTYYCPINWHFTAEEVRFLLA
DSGAKALIVHAGLLAAVREAVPAGIPVLVVADGAEVSGRSEYEAWLAQQAPYDGPRVSPRGHMAYTSGTTGRPKGVLREP
VPLDQLDDQMARARSLVRQTYGIVPGCRALMSAPLYHSAPGSFIQNALQMAERLVLTSRFDAEQVLALVEKHRIDVLYLV
PIMYVRLLKLPPEVRARYDLSSIRFVASTGSPCSPEVKRAMLEWFGPVIHETYASSEAGMITVATPADAAARPGTAGKPV
DDAQLRILDEDGKPCATGEIGLVYVRQPAYPDFTYRNNEAARRAIDRDGLVTLGDMGYVDADGYLFICDRASDMVISGGV
NIYPAEIEHELVRYPGVADCVVFGVPDDEYGERLLAMVQPMPDAAIREEDVIDWLRQRLSGFKVPRNIVIEAQLPRDETG
KLAKRRLRDRYWEGRQRRV

Sequences:

>Translated_499_residues
MATDLWFEDMHRDGAELLDRGARLAGGLRAAGLKEGDVVAVLLRNDPVYVDVVHACRIGGTYYCPINWHFTAEEVRFLLA
DSGAKALIVHAGLLAAVREAVPAGIPVLVVADGAEVSGRSEYEAWLAQQAPYDGPRVSPRGHMAYTSGTTGRPKGVLREP
VPLDQLDDQMARARSLVRQTYGIVPGCRALMSAPLYHSAPGSFIQNALQMAERLVLTSRFDAEQVLALVEKHRIDVLYLV
PIMYVRLLKLPPEVRARYDLSSIRFVASTGSPCSPEVKRAMLEWFGPVIHETYASSEAGMITVATPADAAARPGTAGKPV
DDAQLRILDEDGKPCATGEIGLVYVRQPAYPDFTYRNNEAARRAIDRDGLVTLGDMGYVDADGYLFICDRASDMVISGGV
NIYPAEIEHELVRYPGVADCVVFGVPDDEYGERLLAMVQPMPDAAIREEDVIDWLRQRLSGFKVPRNIVIEAQLPRDETG
KLAKRRLRDRYWEGRQRRV
>Mature_498_residues
ATDLWFEDMHRDGAELLDRGARLAGGLRAAGLKEGDVVAVLLRNDPVYVDVVHACRIGGTYYCPINWHFTAEEVRFLLAD
SGAKALIVHAGLLAAVREAVPAGIPVLVVADGAEVSGRSEYEAWLAQQAPYDGPRVSPRGHMAYTSGTTGRPKGVLREPV
PLDQLDDQMARARSLVRQTYGIVPGCRALMSAPLYHSAPGSFIQNALQMAERLVLTSRFDAEQVLALVEKHRIDVLYLVP
IMYVRLLKLPPEVRARYDLSSIRFVASTGSPCSPEVKRAMLEWFGPVIHETYASSEAGMITVATPADAAARPGTAGKPVD
DAQLRILDEDGKPCATGEIGLVYVRQPAYPDFTYRNNEAARRAIDRDGLVTLGDMGYVDADGYLFICDRASDMVISGGVN
IYPAEIEHELVRYPGVADCVVFGVPDDEYGERLLAMVQPMPDAAIREEDVIDWLRQRLSGFKVPRNIVIEAQLPRDETGK
LAKRRLRDRYWEGRQRRV

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=520, Percent_Identity=25.3846153846154, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI42544132, Length=505, Percent_Identity=24.950495049505, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI122937307, Length=488, Percent_Identity=27.2540983606557, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI38505220, Length=498, Percent_Identity=25.9036144578313, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI187761345, Length=513, Percent_Identity=26.5107212475634, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI187761343, Length=513, Percent_Identity=26.5107212475634, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI115511026, Length=515, Percent_Identity=23.8834951456311, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI58082049, Length=513, Percent_Identity=25.5360623781676, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI157311624, Length=505, Percent_Identity=25.9405940594059, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI157311622, Length=505, Percent_Identity=25.9405940594059, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI28416953, Length=515, Percent_Identity=24.6601941747573, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI13375727, Length=518, Percent_Identity=25.4826254826255, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI8923896, Length=539, Percent_Identity=22.0779220779221, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI253970489, Length=366, Percent_Identity=23.4972677595628, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI195947378, Length=390, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=498, Percent_Identity=26.1044176706827, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI221142682, Length=488, Percent_Identity=25.2049180327869, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1790505, Length=530, Percent_Identity=23.0188679245283, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI145693145, Length=353, Percent_Identity=28.328611898017, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786810, Length=495, Percent_Identity=25.2525252525253, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786529, Length=524, Percent_Identity=25.381679389313, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=484, Percent_Identity=26.0330578512397, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17558820, Length=491, Percent_Identity=26.4765784114053, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17560308, Length=511, Percent_Identity=26.4187866927593, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=497, Percent_Identity=25.9557344064386, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=525, Percent_Identity=24.5714285714286, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=525, Percent_Identity=24.5714285714286, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=525, Percent_Identity=24.5714285714286, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=504, Percent_Identity=25.5952380952381, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=488, Percent_Identity=25.4098360655738, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=488, Percent_Identity=23.7704918032787, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=365, Percent_Identity=22.7397260273973, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71996755, Length=479, Percent_Identity=22.3382045929019, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=295, Percent_Identity=23.728813559322, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=541, Percent_Identity=23.6598890942699, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=541, Percent_Identity=23.6598890942699, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=491, Percent_Identity=24.2362525458248, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323182, Length=537, Percent_Identity=24.0223463687151, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319264, Length=377, Percent_Identity=24.9336870026525, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922652, Length=493, Percent_Identity=27.789046653144, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24648260, Length=498, Percent_Identity=25.9036144578313, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21355181, Length=492, Percent_Identity=26.0162601626016, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21358303, Length=480, Percent_Identity=23.75, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356441, Length=484, Percent_Identity=22.5206611570248, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281365686, Length=502, Percent_Identity=23.5059760956175, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24656500, Length=499, Percent_Identity=24.0480961923848, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648253, Length=494, Percent_Identity=23.0769230769231, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24653035, Length=485, Percent_Identity=23.9175257731959, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356947, Length=489, Percent_Identity=22.6993865030675, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648255, Length=495, Percent_Identity=23.2323232323232, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI18859661, Length=503, Percent_Identity=24.0556660039761, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581924, Length=514, Percent_Identity=23.1517509727626, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161076582, Length=492, Percent_Identity=23.5772357723577, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24667955, Length=532, Percent_Identity=22.3684210526316, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI62472339, Length=532, Percent_Identity=22.3684210526316, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24667959, Length=367, Percent_Identity=24.5231607629428, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21355225, Length=369, Percent_Identity=23.8482384823848, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI20130357, Length=492, Percent_Identity=21.5447154471545, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648257, Length=511, Percent_Identity=23.091976516634, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28573631, Length=533, Percent_Identity=24.953095684803, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20130353, Length=533, Percent_Identity=24.953095684803, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 55044; Mature: 54912

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDLWFEDMHRDGAELLDRGARLAGGLRAAGLKEGDVVAVLLRNDPVYVDVVHACRIGG
CCCCHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCCEEEEEEHHHHCCC
TYYCPINWHFTAEEVRFLLADSGAKALIVHAGLLAAVREAVPAGIPVLVVADGAEVSGRS
EEEEEEEEEECHHHHHHHEECCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCH
EYEAWLAQQAPYDGPRVSPRGHMAYTSGTTGRPKGVLREPVPLDQLDDQMARARSLVRQT
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGIVPGCRALMSAPLYHSAPGSFIQNALQMAERLVLTSRFDAEQVLALVEKHRIDVLYLV
HCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEHHH
PIMYVRLLKLPPEVRARYDLSSIRFVASTGSPCSPEVKRAMLEWFGPVIHETYASSEAGM
HHHHHHHHHCCHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
ITVATPADAAARPGTAGKPVDDAQLRILDEDGKPCATGEIGLVYVRQPAYPDFTYRNNEA
EEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHH
ARRAIDRDGLVTLGDMGYVDADGYLFICDRASDMVISGGVNIYPAEIEHELVRYPGVADC
HHHHHCCCCCEEECCCCEECCCCEEEEECCCCCEEEECCCEEEEHHHHHHHHHCCCCCCE
VVFGVPDDEYGERLLAMVQPMPDAAIREEDVIDWLRQRLSGFKVPRNIVIEAQLPRDETG
EEEECCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHH
KLAKRRLRDRYWEGRQRRV
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ATDLWFEDMHRDGAELLDRGARLAGGLRAAGLKEGDVVAVLLRNDPVYVDVVHACRIGG
CCCHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCCEEEEEEHHHHCCC
TYYCPINWHFTAEEVRFLLADSGAKALIVHAGLLAAVREAVPAGIPVLVVADGAEVSGRS
EEEEEEEEEECHHHHHHHEECCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCH
EYEAWLAQQAPYDGPRVSPRGHMAYTSGTTGRPKGVLREPVPLDQLDDQMARARSLVRQT
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGIVPGCRALMSAPLYHSAPGSFIQNALQMAERLVLTSRFDAEQVLALVEKHRIDVLYLV
HCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEHHH
PIMYVRLLKLPPEVRARYDLSSIRFVASTGSPCSPEVKRAMLEWFGPVIHETYASSEAGM
HHHHHHHHHCCHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
ITVATPADAAARPGTAGKPVDDAQLRILDEDGKPCATGEIGLVYVRQPAYPDFTYRNNEA
EEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHH
ARRAIDRDGLVTLGDMGYVDADGYLFICDRASDMVISGGVNIYPAEIEHELVRYPGVADC
HHHHHCCCCCEEECCCCEECCCCEEEEECCCCCEEEECCCEEEEHHHHHHHHHCCCCCCE
VVFGVPDDEYGERLLAMVQPMPDAAIREEDVIDWLRQRLSGFKVPRNIVIEAQLPRDETG
EEEECCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHH
KLAKRRLRDRYWEGRQRRV
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]