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The map label for this gene is proS [H]

Identifier: 73542636

GI number: 73542636

Start: 3237181

End: 3238902

Strand: Reverse

Name: proS [H]

Synonym: Reut_A2952

Alternate gene names: 73542636

Gene position: 3238902-3237181 (Counterclockwise)

Preceding gene: 73542639

Following gene: 73542635

Centisome position: 85.09

GC content: 65.27

Gene sequence:

>1722_bases
ATGAAAGCCTCGCAATTTTTCATTTCCACCCTGAAGGAAGCCCCCGCCGACGCGGAAATCGTGTCGCACAAGCTCATGAT
GCGCGCGGGCATGATCAAGAAGCTCGGCGCCGGCATCTACAACTACATGCCGGTGGGCCTGCGCGTGATCCGCAAGGTGG
AAAACATCGTCCGCGAGGAAATGAACCGCGCCGGCGCCGTGGAACTGTCGATGCCCGTGATCCAGCCGGCCGAGCTGTGG
CAGGAAACCGGCCGCTGGGACAAGATGGGCCCGGAGCTGCTGCGCCTGAAGGATCGCCACGAGCGCGACTTCGCCGTGCA
ACCGACCTCCGAGGAAGTCGTGACGGACATTGCGCGCAGCGAAATCCGCAGCTACAAGCAGCTGCCGGTCAATTTCTACC
AGATCCAGACCAAGTTCCGCGACGAGCGCCGTCCTCGCTTCGGCATCATGCGCGGCCGCGAATTCACGATGAAGGACGCG
TACTCGTTCGACCGTGACGCCGAGGGCCTGAAGAAGTCGTACGAGAACATGTACGACGCGTACGTGCGCATCTTCCAGCG
CTTCGGCCTGGAATTCCGCGCCGTGGCGGCCGACAACGGCGCCATCGGCGGTTCGGGCTCGCACGAATTCCACGTGATTG
CCGACACCGGCGAAGACGCCATCGTCTACTGCCCGACGTCCGACTACGCCGCCAACATGGAAGCCGCCGAGGCGCTGCCG
CTGGTGGCCGAGCGTGCCGCGCCGACCCAGGCGCTGGAAAAGACCTTCACCCCGGAAAAGGTCAAGTGCGAGCAAGTGGC
CGAGTTCCTGGGGATCCCGCTCGAACGCAACGTCAAGTCGATCGTGCTGGCCAAGGACACCGAGGCCGGCGCAGAGATCT
GGCTGCTGCTGATCCGCGCCGACCACGAGCTGAACGAGGTCAAGGCTTCGAAGGTGCCGGGCCTGGCTGACTTCCGCTTC
GCCACCGAAAACGAGATCGTCGACGCGTTCGGTTCGCCCCCGGGCTACCTGGGCCCGATCGACATGAAGAAGCCGGTCAA
GGTCGTGGCCGACCGCACCGTCGCCAACATGAGCGACTTCTGCTGCGGCGCGAACTATCGCGACTACCACTTCACCGGCG
TCAACTGGGGCCGTGACCTGCCCGAGCCGATCGTTGCCGACCTGCGCAACGTGGTGGCCGGGGACGCCTCGCCGGACGGC
AAGGGCACGCTGGAAATCTGCCGCGGCATCGAAGTGGGCCACGTGTTCATGCTCGGCACGCGTTATTCCGAGTCGATGAA
CGCGACCTTCCTGGACGAGAACGGCAAGACCCAACCGATGCAGATGGGCTGCTACGGTATCGGCGTGACCCGTATCCTTG
GCGCCGCCATCGAGCAGAACTTCGACGAGCGCGGCATCATCTGGCCCGCCGCCATTGCCCCGTTCGAGGTCGTGGTGTGC
CCGGTCGGCTATGACCGCTCCGAAGCCGTCAAGGCCGAGGCTGACCGCATCCACGCCGAGCTGCTGGCTGCCGGCGTGGA
CGTCATCATTGACGACCGTGGCGAGCGCCCCGGCGTGATGTTCGCCGACTGGGAACTGATCGGCGTGCCGCACCGCGTGG
TGGTGGGCGACCGTGGCCTGAAGGAAGGCAAGGTCGAATACCAGGGCCGCCGCGACGCGCAGGCGACGCAAGTGGCCGTG
GCCGACGTGGTCGGCCACGTACGCGGCCAGATCGGCAAGTAA

Upstream 100 bases:

>100_bases
GGGCAGTTTCAGGCAGTTGCCGGCGCGTTCCAGTCGATTATGCGCGGCATGGCTGCCGCCAATTCACCGGGATTTACCGG
TTTCCAGAGAAGACATTCGG

Downstream 100 bases:

>100_bases
GCCCATGCGCGCGGCCCGTCTGGTTCGCACGGCCGGCAGCCTGCTGCTGGCCTTTGCCGCCACTGCCGCGCAGGCTGGCG
CGCAAAAGGAAGAAGATCTG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGIYNYMPVGLRVIRKVENIVREEMNRAGAVELSMPVIQPAELW
QETGRWDKMGPELLRLKDRHERDFAVQPTSEEVVTDIARSEIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDA
YSFDRDAEGLKKSYENMYDAYVRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYCPTSDYAANMEAAEALP
LVAERAAPTQALEKTFTPEKVKCEQVAEFLGIPLERNVKSIVLAKDTEAGAEIWLLLIRADHELNEVKASKVPGLADFRF
ATENEIVDAFGSPPGYLGPIDMKKPVKVVADRTVANMSDFCCGANYRDYHFTGVNWGRDLPEPIVADLRNVVAGDASPDG
KGTLEICRGIEVGHVFMLGTRYSESMNATFLDENGKTQPMQMGCYGIGVTRILGAAIEQNFDERGIIWPAAIAPFEVVVC
PVGYDRSEAVKAEADRIHAELLAAGVDVIIDDRGERPGVMFADWELIGVPHRVVVGDRGLKEGKVEYQGRRDAQATQVAV
ADVVGHVRGQIGK

Sequences:

>Translated_573_residues
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGIYNYMPVGLRVIRKVENIVREEMNRAGAVELSMPVIQPAELW
QETGRWDKMGPELLRLKDRHERDFAVQPTSEEVVTDIARSEIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDA
YSFDRDAEGLKKSYENMYDAYVRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYCPTSDYAANMEAAEALP
LVAERAAPTQALEKTFTPEKVKCEQVAEFLGIPLERNVKSIVLAKDTEAGAEIWLLLIRADHELNEVKASKVPGLADFRF
ATENEIVDAFGSPPGYLGPIDMKKPVKVVADRTVANMSDFCCGANYRDYHFTGVNWGRDLPEPIVADLRNVVAGDASPDG
KGTLEICRGIEVGHVFMLGTRYSESMNATFLDENGKTQPMQMGCYGIGVTRILGAAIEQNFDERGIIWPAAIAPFEVVVC
PVGYDRSEAVKAEADRIHAELLAAGVDVIIDDRGERPGVMFADWELIGVPHRVVVGDRGLKEGKVEYQGRRDAQATQVAV
ADVVGHVRGQIGK
>Mature_573_residues
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGIYNYMPVGLRVIRKVENIVREEMNRAGAVELSMPVIQPAELW
QETGRWDKMGPELLRLKDRHERDFAVQPTSEEVVTDIARSEIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDA
YSFDRDAEGLKKSYENMYDAYVRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYCPTSDYAANMEAAEALP
LVAERAAPTQALEKTFTPEKVKCEQVAEFLGIPLERNVKSIVLAKDTEAGAEIWLLLIRADHELNEVKASKVPGLADFRF
ATENEIVDAFGSPPGYLGPIDMKKPVKVVADRTVANMSDFCCGANYRDYHFTGVNWGRDLPEPIVADLRNVVAGDASPDG
KGTLEICRGIEVGHVFMLGTRYSESMNATFLDENGKTQPMQMGCYGIGVTRILGAAIEQNFDERGIIWPAAIAPFEVVVC
PVGYDRSEAVKAEADRIHAELLAAGVDVIIDDRGERPGVMFADWELIGVPHRVVVGDRGLKEGKVEYQGRRDAQATQVAV
ADVVGHVRGQIGK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=231, Percent_Identity=44.1558441558442, Blast_Score=226, Evalue=4e-59,
Organism=Escherichia coli, GI1786392, Length=572, Percent_Identity=56.4685314685315, Blast_Score=686, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=208, Percent_Identity=40.3846153846154, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=87, Percent_Identity=35.632183908046, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=547, Percent_Identity=29.0676416819013, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=37.5510204081633, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63589; Mature: 63589

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGIYNYMPVGLRVIRKVENIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVELSMPVIQPAELWQETGRWDKMGPELLRLKDRHERDFAVQPTSEEVVTDIARS
HHCCCCEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHH
EIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDAYSFDRDAEGLKKSYENMYDA
HHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHH
YVRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYCPTSDYAANMEAAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHH
LVAERAAPTQALEKTFTPEKVKCEQVAEFLGIPLERNVKSIVLAKDTEAGAEIWLLLIRA
HHHHHCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEEEC
DHELNEVKASKVPGLADFRFATENEIVDAFGSPPGYLGPIDMKKPVKVVADRTVANMSDF
CCHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHH
CCGANYRDYHFTGVNWGRDLPEPIVADLRNVVAGDASPDGKGTLEICRGIEVGHVFMLGT
HCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEEC
RYSESMNATFLDENGKTQPMQMGCYGIGVTRILGAAIEQNFDERGIIWPAAIAPFEVVVC
CCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEE
PVGYDRSEAVKAEADRIHAELLAAGVDVIIDDRGERPGVMFADWELIGVPHRVVVGDRGL
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECEEEECCCCEEEECCCCC
KEGKVEYQGRRDAQATQVAVADVVGHVRGQIGK
CCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGIYNYMPVGLRVIRKVENIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVELSMPVIQPAELWQETGRWDKMGPELLRLKDRHERDFAVQPTSEEVVTDIARS
HHCCCCEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHH
EIRSYKQLPVNFYQIQTKFRDERRPRFGIMRGREFTMKDAYSFDRDAEGLKKSYENMYDA
HHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHH
YVRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYCPTSDYAANMEAAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHH
LVAERAAPTQALEKTFTPEKVKCEQVAEFLGIPLERNVKSIVLAKDTEAGAEIWLLLIRA
HHHHHCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEEEC
DHELNEVKASKVPGLADFRFATENEIVDAFGSPPGYLGPIDMKKPVKVVADRTVANMSDF
CCHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHH
CCGANYRDYHFTGVNWGRDLPEPIVADLRNVVAGDASPDGKGTLEICRGIEVGHVFMLGT
HCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEEC
RYSESMNATFLDENGKTQPMQMGCYGIGVTRILGAAIEQNFDERGIIWPAAIAPFEVVVC
CCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEE
PVGYDRSEAVKAEADRIHAELLAAGVDVIIDDRGERPGVMFADWELIGVPHRVVVGDRGL
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECEEEECCCCEEEECCCCC
KEGKVEYQGRRDAQATQVAVADVVGHVRGQIGK
CCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA