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The map label for this gene is proB [H]

Identifier: 73542639

GI number: 73542639

Start: 3240445

End: 3241563

Strand: Reverse

Name: proB [H]

Synonym: Reut_A2955

Alternate gene names: 73542639

Gene position: 3241563-3240445 (Counterclockwise)

Preceding gene: 73542640

Following gene: 73542636

Centisome position: 85.16

GC content: 66.04

Gene sequence:

>1119_bases
ATGCAATCGGTCATTGCCCAGGCAAAACGTATCGTCGTCAAGGTTGGCTCCAGCCTGGTCACCAACGACGGCAAGGGATT
GGACTACGACGCCATTGCGCGCTGGGCGGCGCAGATCGCAAAGCTGCGTTCGATCGGCAAGGAAGTGGTGCTGGTTAGTT
CCGGCGCCATTGCCGAGGGCATGCAACGCCTGGGCTGGGTACGCCGACCGCGTGAAATCCACGAACTGCAAGCCGCCGCC
GCCGTTGGCCAGATGGGCCTGGCGCAGGTCTACGAAAGCCAGTTCGGCCGCTATGGCATCCGCACCGCGCAGGTGCTGCT
GACCCACGCCGATCTGGCCGACCGCGAACGCTACCTGAACGCACGTTCCACGCTGCTCACGCTGCTGTCTCTCGGCGTCG
TGCCGATCATCAACGAGAACGACACCGTGGTTACCGACGAAATCAAGTTCGGCGACAACGACACGCTTGGCGCGCTGGTC
ACCAACCTGATCGAAGGCGATGCGCTGGTCATCCTGACCGACCAGCGTGGGCTCTACACCGCTGATCCGCGCAAGGACCC
GAATGCCGAGTTCGTCGACGAGGCGCTGGCCGGCACGCCCGAGCTGGAGCAGATGGCGGGCGGCGCCGGTACATCGATCG
GCCGGGGCGGCATGCTGACCAAGATCCTCGCGGCCAAGCGCGCGGCCAAGTCAGGCGCGCACACGACCATTGCGTCTGGC
CGTGAGGCCAACGTGCTCGAACGCCTGGCAGCCGGTGAGGCCATTGGCACGCAACTGCTGGCGCCGACCGGCCGCCTGAC
CGCGCGCAAACAGTGGATGGCAGATCACTTGCAGCTGCGCGGCCGCGTGGTCATCGACGCCGGCGCCGTCGAAAAGCTGA
CCAGCGGCGGCAAGTCACTGCTGCCGATCGGCGTGGTCGAAGTGCAGGGCGAGTTCGCGCGCGGCGAGGTAATTGCCTGT
GTCGGCGCGGACGGGCGCGAAGTGGCGCGCGGCATCACCAACTATTCGAGCGCCGAGGCCCGCCTGATTGCGCGCAAGCC
GTCGTCGGAGATCGAGTCGGTGCTCGGCCACCTGAACGAACCCGAACTGATCCACCGGGACAACCTCGTGCTGGTCTGA

Upstream 100 bases:

>100_bases
GGTCACGAGCAGTAAGCCAGCTTTTCTCCCTCGCCCATGCGGGGCGAGGGATCACCATTGGGGATGCCAGAAAAGCCCCC
CGGAGACAGGATTTCTCGCC

Downstream 100 bases:

>100_bases
GCCGGCATCACGCCGCAAAAGAAAAGGGCGACGCCGAGGCATCGCCCTTTCTGCATTCAGCCGGCTGGCATTCAGCGACG
GTGCGGTGCGCTGTCCTTCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MQSVIAQAKRIVVKVGSSLVTNDGKGLDYDAIARWAAQIAKLRSIGKEVVLVSSGAIAEGMQRLGWVRRPREIHELQAAA
AVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALV
TNLIEGDALVILTDQRGLYTADPRKDPNAEFVDEALAGTPELEQMAGGAGTSIGRGGMLTKILAAKRAAKSGAHTTIASG
REANVLERLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVVIDAGAVEKLTSGGKSLLPIGVVEVQGEFARGEVIAC
VGADGREVARGITNYSSAEARLIARKPSSEIESVLGHLNEPELIHRDNLVLV

Sequences:

>Translated_372_residues
MQSVIAQAKRIVVKVGSSLVTNDGKGLDYDAIARWAAQIAKLRSIGKEVVLVSSGAIAEGMQRLGWVRRPREIHELQAAA
AVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALV
TNLIEGDALVILTDQRGLYTADPRKDPNAEFVDEALAGTPELEQMAGGAGTSIGRGGMLTKILAAKRAAKSGAHTTIASG
REANVLERLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVVIDAGAVEKLTSGGKSLLPIGVVEVQGEFARGEVIAC
VGADGREVARGITNYSSAEARLIARKPSSEIESVLGHLNEPELIHRDNLVLV
>Mature_372_residues
MQSVIAQAKRIVVKVGSSLVTNDGKGLDYDAIARWAAQIAKLRSIGKEVVLVSSGAIAEGMQRLGWVRRPREIHELQAAA
AVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLNARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALV
TNLIEGDALVILTDQRGLYTADPRKDPNAEFVDEALAGTPELEQMAGGAGTSIGRGGMLTKILAAKRAAKSGAHTTIASG
REANVLERLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVVIDAGAVEKLTSGGKSLLPIGVVEVQGEFARGEVIAC
VGADGREVARGITNYSSAEARLIARKPSSEIESVLGHLNEPELIHRDNLVLV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=310, Percent_Identity=32.9032258064516, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI62912457, Length=308, Percent_Identity=33.1168831168831, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=42.0765027322404, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=34.0501792114695, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=34.0501792114695, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=35.9523809523809, Blast_Score=212, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6321822, Length=420, Percent_Identity=33.5714285714286, Blast_Score=192, Evalue=7e-50,
Organism=Drosophila melanogaster, GI21357643, Length=288, Percent_Identity=31.9444444444444, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39561; Mature: 39561

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSVIAQAKRIVVKVGSSLVTNDGKGLDYDAIARWAAQIAKLRSIGKEVVLVSSGAIAEG
CCHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWVRRPREIHELQAAAAVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
ARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALVILTDQRGLYT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNAEFVDEALAGTPELEQMAGGAGTSIGRGGMLTKILAAKRAAKSGAHTTIASG
CCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
REANVLERLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVVIDAGAVEKLTSGGKSL
CHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCC
LPIGVVEVQGEFARGEVIACVGADGREVARGITNYSSAEARLIARKPSSEIESVLGHLNE
CEEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHEEEECCCHHHHHHHHHCCCC
PELIHRDNLVLV
CCEEECCCEEEC
>Mature Secondary Structure
MQSVIAQAKRIVVKVGSSLVTNDGKGLDYDAIARWAAQIAKLRSIGKEVVLVSSGAIAEG
CCHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWVRRPREIHELQAAAAVGQMGLAQVYESQFGRYGIRTAQVLLTHADLADRERYLN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
ARSTLLTLLSLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALVILTDQRGLYT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNAEFVDEALAGTPELEQMAGGAGTSIGRGGMLTKILAAKRAAKSGAHTTIASG
CCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
REANVLERLAAGEAIGTQLLAPTGRLTARKQWMADHLQLRGRVVIDAGAVEKLTSGGKSL
CHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCC
LPIGVVEVQGEFARGEVIACVGADGREVARGITNYSSAEARLIARKPSSEIESVLGHLNE
CEEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHEEEECCCHHHHHHHHHCCCC
PELIHRDNLVLV
CCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA